| |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation F?+21 (08-JAN-08) Donating Investigator Rudolf Jaenisch, Whitehead Institute (MIT) Description
MEFs prepared from the DR-4 mouse strain displayed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above those used normally for the selection of drug-resistant ES cells. This mouse strain represents an economical donor for the production of multiple-drug resistant mouse embryonic fibroblasts (MEFs).Development
The Dr-4 strain of mice was prepared by the intercrossing of three different strains, one bearing resistance genes neoR and puroR a second bearing the resistance gene hygR, and a third bearing a natural deletion encompassing the Hprt gene. A series of matings incorporated all 4 drug resistance genes into the strain. See reference.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Pgk1
002354 C57BL/6J-Tg(pPWL512hyg)1Ems/J 002355 C57BL/6J-Tg(pPWL88puro)2Ems/J View Strains carrying other alleles of Pgk1 (2 strains)
002354 C57BL/6J-Tg(pPWL512hyg)1Ems/J
002356 C57BL/6J-Tg(pPGKneobpA)3Ems/J
002355 C57BL/6J-Tg(pPWL88puro)2Ems/J
Additional Web Information
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
neo relatedResearch Tools
Genetics Research (Mutagenesis and Transgenesis: Production of Targeted Mutations ("Knockouts"))
Reproductive Biology Research (multiple drug resistant embryonic fibroblasts)
Research Tools
Genetics Research (Mutagenesis and Transgenesis: Production of Targeted Mutations ("Knockouts"), feeder cells for ES cell lines)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(DR4)1Jae | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Rudolph Jaenisch | ||
| Allele Type | Not Applicable | ||
| Common Name(s) | DR-4; DR4; | ||
| Mutation Made By | Rudolf Jaenisch, Whitehead Institute (MIT) | ||
| Expressed Gene | hyg, hygromycin resistance, bacteria | ||
| Expressed Gene | neo, neomycin resistance, bacterial | ||
| Expressed Gene | puro, puromycin resistance, | ||
| Promoter | Pgk1, phosphoglycerate kinase 1, rat | ||
| General Note | Mouse embryonic fibroblasts prepared from the DR4 mouse strain displayed resistance to concentrations of four drugs (G418, 6-thioguanine, puromycin, and hygromycin) well above those used normally for the selection of drug resistant ES cells. | ||
| Molecular Note | The symbol Tg(DR4)1Jae represents a combination of three mutations (two transgenes and a knock-in) that together confer resistance to four drugs in STOCK Tg(DR4)1Jae/J mice. The DR4 strain of mice was produced by the intercross of three strains: one strain carries the knock-in Dnmt1tm3Jae, which contains the drug resistance genes neoR and puroR; a second strain carries Tg(pPWL512hyg)1Ems, which contains the resistance gene hygR; and the third strain carries Tg(Hprt1)1Jae, which is a knock-in of the natural deletion Hprt1b-m3 and confers resistance to the drug 6-thioguanine. A series of matings incorporated all 4 drug resistance genes into the DR4 strain. The phosphoglycerate kinase 1 promoter controls expression of each drug resistance gene. [MGI Ref ID J:92995] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(DR4)1Jae, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Tucker KL; Wang Y; Dausman J; Jaenisch R. 1997. A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res 25(18):3745-6. [PubMed: 9278500] [MGI Ref ID J:92995]
Additional References
Tucker KL; Beard C; Dausmann J; Jackson-Grusby L; Laird PW; Lei H; Li E; Jaenisch R. 1996. Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes. Genes Dev 10(8):1008-20. [PubMed: 8608936] [MGI Ref ID J:83179]
Tg(DR4)1Jae relatedHsiao FC; Lin M; Tai A; Chen G; Huber BT. 2006. Cutting edge: Epstein-Barr virus transactivates the HERV-K18 superantigen by docking to the human complement receptor 2 (CD21) on primary B cells. J Immunol 177(4):2056-60. [PubMed: 16887963] [MGI Ref ID J:138376]
Johnson KA; Lerner CP; Di Lacio LC; Laird PW; Sharpe AH; Simpson EM. 1995. Transgenic mice for the preparation of hygromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selections. Nucleic Acids Res 23(7):1273-5. [PubMed: 7739908] [MGI Ref ID J:92459]
Tucker KL; Beard C; Dausmann J; Jackson-Grusby L; Laird PW; Lei H; Li E; Jaenisch R. 1996. Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes. Genes Dev 10(8):1008-20. [PubMed: 8608936] [MGI Ref ID J:83179]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain was generated on a mixed stock (129/SvJae + OlaHsd + BALB/c + C57BL/6). It is currently on the same genetic background. The strain is maintained by breeding homozygotes. Expected coat color from breeding:Albino Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $109.70 Female or Male Homozygous for Tg(DR4)1Jae *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $219.40 Homozygous for Tg(DR4)1Jae x Homozygous for Tg(DR4)1Jae
Additional Supply Details
| Supply Notes |
|
|---|
| Pricing for International shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $142.70 Female or Male Homozygous for Tg(DR4)1Jae *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $285.30 Homozygous for Tg(DR4)1Jae x Homozygous for Tg(DR4)1Jae
Additional Supply Details
| Supply Notes |
|
|---|
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.
No Liability
In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.
MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.
Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.