Strain Name:

STOCK Tg(DR4)1Jae/J

Stock Number:

003208

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Availability:

Repository- Live

Other products are available, see Purchasing Information for Cryopreserved Embryos

Use Restrictions Apply, see Terms of Use
DR-4 transgenic mice harbor three independently segregating loci that collectively express four drug resistance genes, and thus provide a good source of multiple-drug resistant mouse embryonic fibroblasts.

Description

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
GenerationF?+13p+F2 (22-JAN-09)
Generation Definitions
 
Donating InvestigatorDr. Rudolf Jaenisch,   Whitehead Institute (MIT)

Description
These DR-4 mice harbor three independently segregating loci that collectively express four drug resistance genes. The Dnmt1tm3Jae targeted mutation on chromosome 9 confers resistance to both neomycin/G418 and puromycin (while retaining Dnmt1 function). The pPWL512hyg transgene (unknown genomic insertion) results in hygromycin resistance. These three resistance genes are controlled by the promoter and polyadenylation signal of the murine 3-phosphoglycerate kinase gene (Pgk1), which ensures high levels of expression in both embryonic stem (ES) cells and mouse embryonic fibroblasts (MEFs). The Hprtb-m3 mutation on the X chromosome confers resistance to 6-thioguanine (and abolishes endogenous Hprt function).

MEFs prepared from DR-4 mice display resistance to concentrations of the drugs G418, puromycin, 6-thioguanine and hygromycin well above those used normally for the selection of drug-resistant ES cells. This mouse strain represents an economical donor for the production of multiple-drug resistant MEFs.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pgk1
002354   C57BL/6J-Tg(pPWL512hyg)1Ems/J
002355   C57BL/6J-Tg(pPWL88puro)2Ems/J
View Strains carrying other alleles of Pgk1     (2 strains)

Strains carrying other alleles of hyg
002354   C57BL/6J-Tg(pPWL512hyg)1Ems/J
View Strains carrying other alleles of hyg     (1 strain)

View Strains carrying other alleles of neo     (9 strains)

Strains carrying other alleles of puro
002355   C57BL/6J-Tg(pPWL88puro)2Ems/J
View Strains carrying other alleles of puro     (1 strain)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
General Purpose
      multiple-drug resistant mouse embryonic fibroblasts
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Production of Targeted Mutations (Knockouts)
      Mutagenesis and Transgenesis: Production of Targeted Mutations (Knockouts), feeder cells for ES cell lines
Reproductive Biology Research
      multiple drug resistant embryonic fibroblasts

neo related

Research Tools
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Production of Targeted Mutations (Knockouts), feeder cells for ES cell lines

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(DR4)1Jae
Allele Name transgene insertion 1, Rudolf Jaenisch
Allele Type Not Applicable
Common Name(s) DR-4; DR4;
Mutation Made ByDr. Rudolf Jaenisch,   Whitehead Institute (MIT)
Strain of Origin129P2/OlaHsd and 129S4/SvJae and BALB/c and C57BL/6
Expressed Gene hyg, hygromycin resistance, bacteria
Expressed Gene puro, puromycin resistance,
Expressed Gene neo, neomycin resistance, bacterial
Promoter Pgk1, phosphoglycerate kinase 1, rat
Gene Symbol and Name Tg(DR4)1Jae, transgene insertion 1, Rudolf Jaenisch
Chromosome UN
General Note Mouse embryonic fibroblasts prepared from the DR4 mouse strain displayed resistance to concentrations of four drugs (G418, 6-thioguanine, puromycin, and hygromycin) well above those used normally for the selection of drug resistant ES cells.
Molecular Note The symbol Tg(DR4)1Jae represents a combination of three mutations (two transgenes and a knock-in) that together confer resistance to four drugs in STOCK Tg(DR4)1Jae/J mice. The DR4 strain was produced by the intercross of three strains: one strain carries the knock-in Dnmt1tm3Jae, which contains neomycin and puromycin resistance genes; a second strain carries Tg(pPWL512hyg)1Ems, which contains the hygromycin resistance gene; and the third strain carries Tg(Hprt1)1Jae, which is a knock-in of Hprtb-m3 and confers 6-TG resistance (6-thioguanine). A series of matings incorporated all 4 drug resistance genes into the DR4 strain. The phosphoglycerate kinase 1 promoter controls expression of each drug resistance gene. [MGI Ref ID J:92995]
 
 
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Generic Puromycin-QPCR, Separated QPCR
Generic Hygromycin, QPCR
Tg(DR4)1Jae Dnmt,

MELT


Tg(DR4)1Jae Dnmt, Standard PCR
Tg(DR4)1Jae/J HPRT,

MELT


Tg(DR4)1Jae/J HPRT, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Tucker KL; Wang Y; Dausman J; Jaenisch R. 1997. A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res 25(18):3745-6. [PubMed: 9278500]  [MGI Ref ID J:92995]

Additional References

Tucker KL; Beard C; Dausmann J; Jackson-Grusby L; Laird PW; Lei H; Li E; Jaenisch R. 1996. Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes. Genes Dev 10(8):1008-20. [PubMed: 8608936]  [MGI Ref ID J:83179]

Tg(DR4)1Jae related

Hsiao FC; Lin M; Tai A; Chen G; Huber BT. 2006. Cutting edge: Epstein-Barr virus transactivates the HERV-K18 superantigen by docking to the human complement receptor 2 (CD21) on primary B cells. J Immunol 177(4):2056-60. [PubMed: 16887963]  [MGI Ref ID J:138376]

Johnson KA; Lerner CP; Di Lacio LC; Laird PW; Sharpe AH; Simpson EM. 1995. Transgenic mice for the preparation of hygromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selections. Nucleic Acids Res 23(7):1273-5. [PubMed: 7739908]  [MGI Ref ID J:92459]

Tucker KL; Beard C; Dausmann J; Jackson-Grusby L; Laird PW; Lei H; Li E; Jaenisch R. 1996. Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes. Genes Dev 10(8):1008-20. [PubMed: 8608936]  [MGI Ref ID J:83179]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryThese DR-4 mice harbor three independently segregating loci that collectively express four drug resistance genes. When maintaining a live colony, females homozygous for the Dnmt1tm3Jae allele, homozygous for the X-linked Hprtb-m3 allele, and homozygous for the pPWL512hyg transgene may be bred with males homozygous for the Dnmt1tm3Jae allele, hemizygous for the X-linked Hprtb-m3 allele, and homozygous for the pPWL512hyg transgene. The expected coat color is albino.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHomozygous for Tg(DR4)1Jae  
Price per Pair (US dollars $)Pair Genotype
$478.00Homozygous for Tg(DR4)1Jae x Homozygous for Tg(DR4)1Jae  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Cryopreserved

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHomozygous for Tg(DR4)1Jae  
Price per Pair (US dollars $)Pair Genotype
$621.40Homozygous for Tg(DR4)1Jae x Homozygous for Tg(DR4)1Jae  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Cryopreserved

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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