Strain Name:

C57BL/6-Tg(Crh)227.1Pbl/J

Stock Number:

003210

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Terms of Use

Description

Strain Information

Former Names C57BL/6-Tg(CRF)227.1Pbl/J    (Changed: 15-DEC-04 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
GenerationN?+2
 
Donating Investigator Wylie Vale,   Salk Institute for Biological Studies

Description
These animals serve as a model for Cushing's Syndrome. They exhibit elevated ACTH release, high circulating levels of CRF and adrenal corticosterone. They display excess fat accumulation, muscle atrophy, thin skin and alopecia. They may provide a model for clinical depression, anorexia nervosa and immune dysfunction, syndromes that result from alterations in CRF levels.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Mt1     (14 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects

Mt1 related

Metabolism Research

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Crh)227.1Pbl
Allele Name transgene insertion 227.1, Wylie W Vale
Allele Type Transgenic (random, expressed)
Common Name(s) CRF Tg; CRH-th; Tg(CRF)227.1Pbl;
Mutation Made By M.P. Stenzel-Poore,   The Salk Inst for Biological Studies
Strain of OriginC57BL/6 x SJL
Expressed Gene Crh, corticotropin releasing hormone, rat
Promoter Mt1, metallothionein 1, mouse, laboratory
General Note On a C57BL/6 background transgenic mice exhibit elevated ACTH release, high circulating levels of corticotropin releasing hormone (CRH), and adrenal corticosterone. They display excess fat accumulation, muscle atrophy, thin skin, and alopecia.
Molecular Note The transgene contains the rat corticotropin releasing hormone (Crh) coding region driven by the murine metallothionine 1 promoter (Mt1). [MGI Ref ID J:92996]

Genotyping

Genotyping Information

Genotyping Protocols

Human Growth Hormone Polyadenylation Signal Sequence, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Stenzel-Poore MP; Cameron VA; Vaughan J; Sawchenko PE; Vale W. 1992. Development of Cushing's syndrome in corticotropin-releasing factor transgenic mice. Endocrinology 130(6):3378-86. [PubMed: 1597149]  [MGI Ref ID J:92996]

Additional References

Tg(Crh)227.1Pbl related

Heinrichs SC; Min H; Tamraz S; Carmouche M; Boehme SA; Vale WW. 1997. Anti-sexual and anxiogenic behavioral consequences of corticotropin-releasing factor overexpression are centrally mediated. Psychoneuroendocrinology 22(4):215-24. [PubMed: 9226726]  [MGI Ref ID J:127677]

Murray SE; Lallman HR; Heard AD; Rittenberg MB; Stenzel-Poore MP. 2001. A genetic model of stress displays decreased lymphocytes and impaired antibody responses without altered susceptibility to Streptococcus pneumoniae. J Immunol 167(2):691-8. [PubMed: 11441072]  [MGI Ref ID J:107150]

Murray SE; Rosenzweig HL; Johnson M; Huising MO; Sawicki K; Stenzel-Poore MP. 2004. Overproduction of corticotropin-releasing hormone blocks germinal center formation: role of corticosterone and impaired follicular dendritic cell networks. J Neuroimmunol 156(1-2):31-41. [PubMed: 15465594]  [MGI Ref ID J:132461]

Palmer AA; Sharpe AL; Burkhart-Kasch S; McKinnon CS; Coste SC; Stenzel-Poore MP; Phillips TJ. 2004. Corticotropin-releasing factor overexpression decreases ethanol drinking and increases sensitivity to the sedative effects of ethanol. Psychopharmacology (Berl) 176(3-4):386-97. [PubMed: 15138758]  [MGI Ref ID J:127871]

Tovote P; Meyer M; Ronnenberg A; Ogren SO; Spiess J; Stiedl O. 2005. Heart rate dynamics and behavioral responses during acute emotional challenge in corticotropin-releasing factor receptor 1-deficient and corticotropin-releasing factor-overexpressing mice. Neuroscience 134(4):1113-22. [PubMed: 16039799]  [MGI Ref ID J:104425]

van Gaalen MM; Reul JH; Gesing A; Stenzel-Poore MP; Holsboer F; Steckler T. 2002. Mice overexpressing CRH show reduced responsiveness in plasma corticosterone after a5-HT1A receptor challenge. Genes Brain Behav 1(3):174-7. [PubMed: 12884973]  [MGI Ref ID J:130551]

van Gaalen MM; Stenzel-Poore M; Holsboer F; Steckler T. 2003. Reduced attention in mice overproducing corticotropin-releasing hormone. Behav Brain Res 142(1-2):69-79. [PubMed: 12798267]  [MGI Ref ID J:130993]

van Gaalen MM; Stenzel-Poore MP; Holsboer F; Steckler T. 2002. Effects of transgenic overproduction of CRH on anxiety-like behaviour. Eur J Neurosci 15(12):2007-15. [PubMed: 12099906]  [MGI Ref ID J:128161]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries

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phone:207-288-6470
fax:207-288-6655

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