Strain Name:

C57BL/6-Tg(Crh)227.1Pbl/J

Stock Number:

003210

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/6-Tg(CRF)227.1Pbl/J    (Changed: 15-DEC-04 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Wylie W. Vale,   Salk Institute for Biological Studies

Description
These animals serve as a model for Cushing's Syndrome. They exhibit elevated ACTH release, high circulating levels of CRF and adrenal corticosterone. They display excess fat accumulation, muscle atrophy, thin skin and alopecia. They may provide a model for clinical depression, anorexia nervosa and immune dysfunction, syndromes that result from alterations in CRF levels.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Mt1     (16 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.
Acth-Independent Macronodular Adrenal Hyperplasia; AIMAH
Obesity
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Crh)227.1Pbl/0

        C57BL/6
  • endocrine/exocrine gland phenotype
  • enlarged adrenal glands
    • transgenic mice exhibit increased adrenal weights   (MGI Ref ID J:92996)
  • hypersecretion of adrenocorticotropin
    • transgenic mice exhibit elevated ACTH release   (MGI Ref ID J:92996)
  • homeostasis/metabolism phenotype
  • increased circulating adrenocorticotropin level
    • transgenic mice show a 5-fold elevation in serum levels of ACTH   (MGI Ref ID J:92996)
  • increased circulating corticosterone level
    • notably, addition of zinc in the drinking water does not further increase the corticosterone levels in founder mice   (MGI Ref ID J:92996)
    • transgenic mice show up to a 10-fold increase in corticosterone levels relative to control non-transgenic littermates; the degree of endocrine dysfunction varies widely among founders   (MGI Ref ID J:92996)
  • increased circulating corticotropin-releasing hormone level   (MGI Ref ID J:92996)
  • growth/size/body phenotype
  • abnormal postnatal growth
    • transgenic mice display decreased linear growth relative to non-transgenic littermates   (MGI Ref ID J:92996)
  • obese
    • transgenic mice exhibit truncal obesity with large adipose deposits   (MGI Ref ID J:92996)
  • muscle phenotype
  • muscular atrophy   (MGI Ref ID J:92996)
  • reproductive system phenotype
  • reduced female fertility
    • transgenic females show decreased fertility probably due to increased corticosterone levels or CRF levels in the brain   (MGI Ref ID J:92996)
  • hematopoietic system phenotype
  • decreased spleen weight
    • transgenic mice show a 3-fold reduction in spleen weight   (MGI Ref ID J:92996)
  • decreased thymus weight
    • transgenic mice show a ~2-fold reduction in thymus weight   (MGI Ref ID J:92996)
  • immune system phenotype
  • decreased spleen weight
    • transgenic mice show a 3-fold reduction in spleen weight   (MGI Ref ID J:92996)
  • decreased thymus weight
    • transgenic mice show a ~2-fold reduction in thymus weight   (MGI Ref ID J:92996)
  • nervous system phenotype
  • hypersecretion of adrenocorticotropin
    • transgenic mice exhibit elevated ACTH release   (MGI Ref ID J:92996)
  • integument phenotype
  • alopecia
    • transgenic mice display bilateral symmetric hair loss   (MGI Ref ID J:92996)
  • thin skin   (MGI Ref ID J:92996)
  • translucent skin
    • transgenic mice show abnormally transparent skin   (MGI Ref ID J:92996)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Crh)227.1Pbl
Allele Name transgene insertion 227.1, Wylie W Vale
Allele Type Transgenic (random, expressed)
Common Name(s) CRF Tg; CRF-OE; CRH-th; Tg(CRF)227.1Pbl; Tg(Crh);
Mutation Made ByDr. M.P. Stenzel-Poore,   The Salk Inst for Biological Studies
Strain of OriginC57BL/6 x SJL
Expressed Gene Crh, corticotropin releasing hormone, rat
Promoter Mt1, metallothionein 1, mouse, laboratory
Molecular Note The transgene contains the rat corticotropin releasing hormone (Crh) coding region driven by the murine metallothionine 1 promoter (Mt1). [MGI Ref ID J:92996]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Crh), Melt Curve Analysis
Tg(Crh), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Stenzel-Poore MP; Cameron VA; Vaughan J; Sawchenko PE; Vale W. 1992. Development of Cushing's syndrome in corticotropin-releasing factor transgenic mice. Endocrinology 130(6):3378-86. [PubMed: 1597149]  [MGI Ref ID J:92996]

Additional References

Tg(Crh)227.1Pbl related

Carroll JC; Iba M; Bangasser DA; Valentino RJ; James MJ; Brunden KR; Lee VM; Trojanowski JQ. 2011. Chronic Stress Exacerbates Tau Pathology, Neurodegeneration, and Cognitive Performance through a Corticotropin-Releasing Factor Receptor-Dependent Mechanism in a Transgenic Mouse Model of Tauopathy. J Neurosci 31(40):14436-49. [PubMed: 21976528]  [MGI Ref ID J:177434]

Goebel M; Fleming SM; Million M; Stengel A; Tache Y; Wang L. 2010. Mice overexpressing corticotropin-releasing factor show brain atrophy and motor dysfunctions. Neurosci Lett 473(1):11-5. [PubMed: 20132869]  [MGI Ref ID J:159921]

Gourcerol G; Wang L; Adelson DW; Larauche M; Tache Y; Million M. 2009. Cholinergic giant migrating contractions in conscious mouse colon assessed by using a novel noninvasive solid-state manometry method: modulation by stressors. Am J Physiol Gastrointest Liver Physiol 296(5):G992-G1002. [PubMed: 19299579]  [MGI Ref ID J:149614]

Heinrichs SC; Min H; Tamraz S; Carmouche M; Boehme SA; Vale WW. 1997. Anti-sexual and anxiogenic behavioral consequences of corticotropin-releasing factor overexpression are centrally mediated. Psychoneuroendocrinology 22(4):215-24. [PubMed: 9226726]  [MGI Ref ID J:127677]

Murray SE; Lallman HR; Heard AD; Rittenberg MB; Stenzel-Poore MP. 2001. A genetic model of stress displays decreased lymphocytes and impaired antibody responses without altered susceptibility to Streptococcus pneumoniae. J Immunol 167(2):691-8. [PubMed: 11441072]  [MGI Ref ID J:107150]

Murray SE; Rosenzweig HL; Johnson M; Huising MO; Sawicki K; Stenzel-Poore MP. 2004. Overproduction of corticotropin-releasing hormone blocks germinal center formation: role of corticosterone and impaired follicular dendritic cell networks. J Neuroimmunol 156(1-2):31-41. [PubMed: 15465594]  [MGI Ref ID J:132461]

Palmer AA; Sharpe AL; Burkhart-Kasch S; McKinnon CS; Coste SC; Stenzel-Poore MP; Phillips TJ. 2004. Corticotropin-releasing factor overexpression decreases ethanol drinking and increases sensitivity to the sedative effects of ethanol. Psychopharmacology (Berl) 176(3-4):386-97. [PubMed: 15138758]  [MGI Ref ID J:127871]

Shinahara M; Nishiyama M; Iwasaki Y; Nakayama S; Noguchi T; Kambayashi M; Okada Y; Tsuda M; Stenzel-Poore MP; Hashimoto K; Terada Y. 2009. Plasma adiponectin levels are increased despite insulin resistance in corticotropin-releasing hormone transgenic mice, an animal model of Cushing syndrome. Endocr J 56(7):879-86. [PubMed: 19550079]  [MGI Ref ID J:156565]

Stengel A; Goebel M; Million M; Stenzel-Poore MP; Kobelt P; Monnikes H; Tache Y; Wang L. 2009. Corticotropin-releasing factor-overexpressing mice exhibit reduced neuronal activation in the arcuate nucleus and food intake in response to fasting. Endocrinology 150(1):153-60. [PubMed: 18787020]  [MGI Ref ID J:158113]

Tovote P; Meyer M; Ronnenberg A; Ogren SO; Spiess J; Stiedl O. 2005. Heart rate dynamics and behavioral responses during acute emotional challenge in corticotropin-releasing factor receptor 1-deficient and corticotropin-releasing factor-overexpressing mice. Neuroscience 134(4):1113-22. [PubMed: 16039799]  [MGI Ref ID J:104425]

Wallace K; Flecknell PA; Burt AD; Wright MC. 2010. Disrupted pancreatic exocrine differentiation and malabsorption in response to chronic elevated systemic glucocorticoid. Am J Pathol 177(3):1225-32. [PubMed: 20651242]  [MGI Ref ID J:163448]

Wallace K; Long Q; Fairhall EA; Charlton KA; Wright MC. 2011. Serine/threonine protein kinase SGK1 in glucocorticoid-dependent transdifferentiation of pancreatic acinar cells to hepatocytes. J Cell Sci 124(Pt 3):405-13. [PubMed: 21224398]  [MGI Ref ID J:182884]

Wanat MJ; Hopf FW; Stuber GD; Phillips PE; Bonci A. 2008. Corticotropin-releasing factor increases mouse ventral tegmental area dopamine neuron firing through a protein kinase C-dependent enhancement of Ih. J Physiol 586(8):2157-70. [PubMed: 18308824]  [MGI Ref ID J:176400]

Wang L; Million M; Rivier J; Rivier C; Craft N; Stenzel-Poore MP; Tache Y. 2011. CRF receptor antagonist astressin-B reverses and prevents alopecia in CRF over-expressing mice. PLoS One 6(2):e16377. [PubMed: 21359208]  [MGI Ref ID J:171079]

van Gaalen MM; Reul JH; Gesing A; Stenzel-Poore MP; Holsboer F; Steckler T. 2002. Mice overexpressing CRH show reduced responsiveness in plasma corticosterone after a5-HT1A receptor challenge. Genes Brain Behav 1(3):174-7. [PubMed: 12884973]  [MGI Ref ID J:130551]

van Gaalen MM; Stenzel-Poore M; Holsboer F; Steckler T. 2003. Reduced attention in mice overproducing corticotropin-releasing hormone. Behav Brain Res 142(1-2):69-79. [PubMed: 12798267]  [MGI Ref ID J:130993]

van Gaalen MM; Stenzel-Poore MP; Holsboer F; Steckler T. 2002. Effects of transgenic overproduction of CRH on anxiety-like behaviour. Eur J Neurosci 15(12):2007-15. [PubMed: 12099906]  [MGI Ref ID J:128161]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry3/31/00: per Debbie Mayo: Inbred X +/- only. per jjs 4/30/98: We have assigned C57BL/6 as the background based on the information that transgenic males have been backcrossed to C57BL/6 for two years. Different people have been in charge of the colony during that time and no definitive records are easily available. Assumption is N=5 at least.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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