Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Homozygote         (Female x Male)   27-APR-10 Species laboratory mouse Background Strain C57BL/6 Donor Strain 129S7 via ABI 2.1 ES cell line Generation N8N1F5 (24-MAR-11) Generation Definitions   Donating Investigator Dr. Arthur Beaudet,   Baylor College of Medicine

Description
Mice homozygous for the Chrna7^tm1Bay mutation are viable and fertile. Neuropathological and histochemical assessment of the brain reveals no abnormalities. High-affinity nicotine binding sites are present but there is an absence of high-affinity [I-125] alpha-bungarotoxin sites. Homozygotes lack rapidly desensitizing, methyllycaconitine-sensitive, nicotinic currents that are present in hippocampal neurons.

Development
A null mutation of the alpha7 subunit was prepared by deleting the last three exons (8-10) of the Chrna7 gene. Mice deficient in the alpha 7 subunit were generated by introducing a 7 kb deletion into ES cells. The strain originated on a mixed 129/SvEv and C57BL/6 background. The donating investigator backcrossed these mice to C57BL/6 for approximately eight generations ("N8F8") prior to sending to The Jackson Laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed genetic background.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Chrna7^tm1Bay/Chrna7^tm1Bay

        B6.129S7-Chrna7^tm1Bay/J

• reproductive system phenotype
• *normal* reproductive system phenotype
  • female homozygotes have normal ovaries and enter puberty at the expected age, with no significant differences in the onset of vaginal opening or cornification relative to wild-type or heterozygous control females   (MGI Ref ID J:92540)
  • male homozygotes are fertile and exhibit an apparently normal mating behavior   (MGI Ref ID J:92540)
  • testicular weight, total sperm number, and sperm morphology appear normal   (MGI Ref ID J:115434)
• • asthenozoospermia
    • mutant sperm initially appear to swim normally but fail to maintain progressive forward motility   (MGI Ref ID J:115434)
    • only few mutant sperm retain rapid swimming velocities and display hyperactivated swimming patterns following in vitro capacitation   (MGI Ref ID J:115434)
    • many nonmotile sperm exhibit a quivering head and midpiece but no forward movement, confirming that cells are alive   (MGI Ref ID J:115434)
    • however, no significant differences in sperm viability, senescence rate or spontaneous acrosome reaction rate are observed between mutant and wild-type sperm before and after capacitation   (MGI Ref ID J:115434)
  • decreased litter size
    • litters born to homozygous mutant females are significantly smaller than those born to heterozygous females regardless of the male genotype   (MGI Ref ID J:92540)
  • prolonged estrous cycle
    • female homozygotes show asynchronous, prolonged estrous cycles, with an average cycle length of 7.1 ± 0.68 days relative to wild-type and heterozygous females (4.3 ± 0.19 and 4.3 ± 0.10 days, respectively)   (MGI Ref ID J:92540)
    • the extent of asynchrony is variable within the mutant female population, but for a given animal, the average cycle length tends to remain stable at young mature ages (4-6 months) and middle ages (10-14 months)   (MGI Ref ID J:92540)
    • all female homozygotes eventually complete an estrous cycle, and may achieve pregnancy but with a smaller number of surviving pups   (MGI Ref ID J:92540)
  • reduced female fertility
    • the number of live births is significantly less from matings of female homozygotes with either heterozygous (4.5 ± 2.12) or homozygous (3.7 ± 0.53) mutant males in comparison to matings of heterozygous females and males (7.34 ± 0.04) and matings of heterozygous females and homozygous mutant males (7.1 ± 0.40)   (MGI Ref ID J:92540)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Chrna7^tm1Bay/Chrna7^tm1Bay

        involves: 129S7/SvEvBrd * C57BL/6J

• nervous system phenotype
• *normal* nervous system phenotype
  • homozygotes display normal growth, survival, gait, and anatomy and show no developmental abnormalities in the nervous system   (MGI Ref ID J:44288)
• • abnormal brain morphology
    • homozygotes lack high-affinity [125I]alpha-bungarotoxin binding sites   (MGI Ref ID J:44288)
    • however, autoradiography with [3H]nicotine revealed normal levels of high-affinity nicotine binding   (MGI Ref ID J:44288)
  • abnormal nicotine-mediated receptor currents
    • hippocampal neurons from mutant mice do not exhibit rapidly desensitizing, methyllycaconitine-sensitive nicotinic currents   (MGI Ref ID J:44288)
• reproductive system phenotype
• reduced fertility
  • homozygotes do not breed well   (MGI Ref ID J:44288)
• hematopoietic system phenotype
• abnormal myeloblast morphology/development
  • fewer myeloblasts and metamyeloblasts in bone marrow   (MGI Ref ID J:140280)
• decreased erythroblast number
  • fewer erythroblasts and pro-normoblasts found in bone marrow   (MGI Ref ID J:140280)
• decreased neutrophil cell number
  • fewer band neutrophils in bone marrow   (MGI Ref ID J:140280)
• increased lymphocyte cell number
  • increased lymphocytes in bone marrow   (MGI Ref ID J:140280)
• immune system phenotype
• decreased neutrophil cell number
  • fewer band neutrophils in bone marrow   (MGI Ref ID J:140280)
• increased lymphocyte cell number
  • increased lymphocytes in bone marrow   (MGI Ref ID J:140280)

Chrna7^tm1Bay/Chrna7^tm1Bay

        B6.Cg-Chrna7^tm1Bay

• behavior/neurological phenotype
• *normal* behavior/neurological phenotype
  • homozygotes exhibit normal base-line learning and memory performance, and normal sensorimotor gating relative to wild-type   (MGI Ref ID J:51149)
  • also, homozygotes are behaviorally similar to wild-type mice in a wide variety of behavioral tests   (MGI Ref ID J:51149)
  • significant gender differences are noted in several tests; however, there is no evidence suggesting that these gender differences are genotype-specific   (MGI Ref ID J:51149)
• • abnormal seizure response to pharmacological agent
    • homozygotes and wild-type mice display a similar dose-response curve for nicotine-elicited clonic-tonic seizures   (MGI Ref ID J:74703)
    • neither cholinergic compensatory mechanisms nor alterations in binding levels or affinity for nicotinic ligands, such as epibatidine or nicotine, can account for the retained sensitivity to the convulsant effects of nicotine   (MGI Ref ID J:74703)
  • abnormal spatial learning
    • homozygotes locate the hidden platform in the Morris water task significantly faster than wild-type; however, this is a subtle difference and is not supported by the distance traveled data   (MGI Ref ID J:51149)
    • also, homozygotes and wild-type mice show a comparable search behavior during the probe trials   (MGI Ref ID J:51149)
  • decreased anxiety-related response
    • homozygotes spend a significantly greater proportion of their total distance traveled in the open field in the center of the arena relative to wild-type   (MGI Ref ID J:51149)
    • however, this difference in altered anxiety-related behavioral response is not corroborated by the light<->dark exploration test   (MGI Ref ID J:51149)
• immune system phenotype
• abnormal interleukin level
  • endotoxemic homozygotes produce significantly higher levels of IL-1 and IL-6   (MGI Ref ID J:89610)
• abnormal tumor necrosis factor level
  • following endotoxin administration, homozygotes show significantly higher levels of serum TNF levels as well as higher TNF production in the liver and spleen relative to wild-type   (MGI Ref ID J:89610)
  • mutant macrophages are refractory to cholinergic agonists and generate TNF normally in the presence of nicotine or acetylcholine   (MGI Ref ID J:89610)
  • electrical stimulation of the vagus nerve inhibits endotoxin-induced serum TNF levels in wild-type mice, but fails to reduce serum TNF levels in endotoxemic homozygotes   (MGI Ref ID J:89610)
• nervous system phenotype
• abnormal seizure response to pharmacological agent
  • homozygotes and wild-type mice display a similar dose-response curve for nicotine-elicited clonic-tonic seizures   (MGI Ref ID J:74703)
  • neither cholinergic compensatory mechanisms nor alterations in binding levels or affinity for nicotinic ligands, such as epibatidine or nicotine, can account for the retained sensitivity to the convulsant effects of nicotine   (MGI Ref ID J:74703)
• homeostasis/metabolism phenotype
• abnormal interleukin level
  • endotoxemic homozygotes produce significantly higher levels of IL-1 and IL-6   (MGI Ref ID J:89610)
• abnormal tumor necrosis factor level
  • following endotoxin administration, homozygotes show significantly higher levels of serum TNF levels as well as higher TNF production in the liver and spleen relative to wild-type   (MGI Ref ID J:89610)
  • mutant macrophages are refractory to cholinergic agonists and generate TNF normally in the presence of nicotine or acetylcholine   (MGI Ref ID J:89610)
  • electrical stimulation of the vagus nerve inhibits endotoxin-induced serum TNF levels in wild-type mice, but fails to reduce serum TNF levels in endotoxemic homozygotes   (MGI Ref ID J:89610)

Chrna7^tm1Bay/Chrna7^tm1Bay

        involves: 129S7/SvEvBrd * C57BL/6

• respiratory system phenotype
• abnormal airway basal cell differentiation
  • after polidocanol-induced acute injury, 8-wk old mutant mice display delayed regeneration of the tracheal epithelium with a transient hyperplasia of basal cells observed on days 4 and 6, but not on day 8, after injury   (MGI Ref ID J:154705)
• airway basal cell hyperplasia
  • at 1 year of age, the % of 1-2 layers of basal cells in the tracheal epithelium is reduced whereas the % of 3-4 layers of basal cells in increased by 3.8-fold relative to that in wild-type controls   (MGI Ref ID J:154705)
• cellular phenotype
• abnormal airway basal cell differentiation
  • after polidocanol-induced acute injury, 8-wk old mutant mice display delayed regeneration of the tracheal epithelium with a transient hyperplasia of basal cells observed on days 4 and 6, but not on day 8, after injury   (MGI Ref ID J:154705)
• immune system phenotype
• *normal* immune system phenotype
  • IgM levels of preimmune serum are little altered from control levels   (MGI Ref ID J:103908)
• • decreased immunoglobulin level
    • significantly reduced preimmune serum Ig levels   (MGI Ref ID J:103908)
  • • decreased IgG level   (MGI Ref ID J:103908)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Channel and Transporter Defects
      sodium/potassium
Receptor Defects
      cholinergic receptor

Chrna7^tm1Bay related

Neurobiology Research
Receptor Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Chrna7tm1Bay
Allele Name		targeted mutation 1, Baylor College of Medicine
Allele Type		Targeted (knock-out)
Common Name(s)		Acralpha7 -; alpha7; alpha7 NNR -;
Mutation Made By		Dr. Arthur Beaudet,   Baylor College of Medicine
Strain of Origin		129S7/SvEvBrd-Hprt
ES Cell Line Name		AB2.1
ES Cell Line Strain		129S7/SvEvBrd-Hprt
Gene Symbol and Name		Chrna7, cholinergic receptor, nicotinic, alpha polypeptide 7
Chromosome		7
Gene Common Name(s)		Acra7; BTX; CHRNA7-2; NACHRA7; NARAD; acetylcholine receptor alpha 7 neural; alpha7; alpha7 nicotinic receptor; alpha7-nAChR;
Molecular Note		A 7 kb genomic fragment containing exons 8-10 was replaced with a neomycin selection cassette. The deleted sequences encode the second through the fourth transmembrane domains and the cytoplasmic loop. Northern blot analysis on brain samples derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on brain extracts derived from homozygous mice also confirmed that no detectable protein was made from this allele. [MGI Ref ID J:122939] [MGI Ref ID J:44288]

Genotyping

Genotyping Information

Genotyping Protocols

Chrna7^tm1Bay,

Separated MCA

Chrna7^tm1Bay, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Orr-Urtreger A; Goldner FM; Saeki M; Lorenzo I; Goldberg L; De Biasi M ; Dani JA ; Patrick JW ; Beaudet AL. 1997. Mice deficient in the alpha7 neuronal nicotinic acetylcholine receptor lack alpha-bungarotoxin binding sites and hippocampal fast nicotinic currents. J Neurosci 17(23):9165-71. [PubMed: 9364063]  [MGI Ref ID J:44288]

Additional References

Wang H; Yu M; Ochani M; Amella CA; Tanovic M; Susarla S; Li JH; Wang H; Yang H; Ulloa L; Al-Abed Y; Czura CJ; Tracey KJ. 2003. Nicotinic acetylcholine receptor alpha7 subunit is an essential regulator of inflammation. Nature 421(6921):384-8. [PubMed: 12508119]  [MGI Ref ID J:89610]

Chrna7^tm1Bay related

Adams CE; Yonchek JC; Schulz KM; Graw SL; Stitzel J; Teschke PU; Stevens KE. 2012. Reduced Chrna7 expression in mice is associated with decreases in hippocampal markers of inhibitory function: implications for neuropsychiatric diseases. Neuroscience 207:274-82. [PubMed: 22314319]  [MGI Ref ID J:184625]

Adams CE; Yonchek JC; Zheng L; Collins AC; Stevens KE. 2008. Altered hippocampal circuit function in C3H alpha7 null mutant heterozygous mice. Brain Res 1194:138-45. [PubMed: 18199426]  [MGI Ref ID J:131902]

An MC; Lin W; Yang J; Dominguez B; Padgett D; Sugiura Y; Aryal P; Gould TW; Oppenheim RW; Hester ME; Kaspar BK; Ko CP; Lee KF. 2010. Acetylcholine negatively regulates development of the neuromuscular junction through distinct cellular mechanisms. Proc Natl Acad Sci U S A 107(23):10702-7. [PubMed: 20498043]  [MGI Ref ID J:161300]

Arredondo J; Nguyen VT; Chernyavsky AI; Bercovich D; Orr-Urtreger A; Kummer W; Lips K; Vetter DE; Grando SA. 2002. Central role of alpha7 nicotinic receptor in differentiation of the stratified squamous epithelium. J Cell Biol 159(2):325-36. [PubMed: 12391028]  [MGI Ref ID J:149058]

Barkis WB; Ford KJ; Feller MB. 2010. Non-cell-autonomous factor induces the transition from excitatory to inhibitory GABA signaling in retina independent of activity. Proc Natl Acad Sci U S A 107(51):22302-7. [PubMed: 21135238]  [MGI Ref ID J:167297]

Bitner RS; Nikkel AL; Markosyan S; Otte S; Puttfarcken P; Gopalakrishnan M. 2009. Selective alpha7 nicotinic acetylcholine receptor activation regulates glycogen synthase kinase3beta and decreases tau phosphorylation in vivo. Brain Res 1265:65-74. [PubMed: 19230830]  [MGI Ref ID J:148203]

Bray C; Son JH; Kumar P; Meizel S. 2005. Mice deficient in CHRNA7, a subunit of the nicotinic acetylcholine receptor, produce sperm with impaired motility. Biol Reprod 73(4):807-14. [PubMed: 15944242]  [MGI Ref ID J:115434]

Brown KL; Comalli DM; De Biasi M; Woodruff-Pak DS. 2010. Trace eyeblink conditioning is impaired in alpha7 but not in beta2 nicotinic acetylcholine receptor knockout mice. Front Behav Neurosci 4:166. [PubMed: 20976039]  [MGI Ref ID J:180417]

Caffery PM; Krishnaswamy A; Sanders T; Liu J; Hartlaub H; Klysik J; Cooper E; Hawrot E. 2009. Engineering neuronal nicotinic acetylcholine receptors with functional sensitivity to alpha-bungarotoxin: a novel alpha3-knock-in mouse. Eur J Neurosci 30(11):2064-76. [PubMed: 20128845]  [MGI Ref ID J:157611]

Campbell NR; Fernandes CC; Halff AW; Berg DK. 2010. Endogenous signaling through alpha7-containing nicotinic receptors promotes maturation and integration of adult-born neurons in the hippocampus. J Neurosci 30(26):8734-44. [PubMed: 20592195]  [MGI Ref ID J:161848]

Chi F; Wang L; Zheng X; Wu CH; Jong A; Sheard MA; Shi W; Huang SH. 2011. Meningitic Escherichia coli K1 penetration and neutrophil transmigration across the blood-brain barrier are modulated by alpha7 nicotinic receptor. PLoS One 6(9):e25016. [PubMed: 21966399]  [MGI Ref ID J:177659]

David R; Ciuraszkiewicz A; Simeone X; Orr-Urtreger A; Papke RL; McIntosh JM; Huck S; Scholze P. 2010. Biochemical and functional properties of distinct nicotinic acetylcholine receptors in the superior cervical ganglion of mice with targeted deletions of nAChR subunit genes. Eur J Neurosci 31(6):978-93. [PubMed: 20377613]  [MGI Ref ID J:159607]

Davis JA; Gould TJ. 2007. beta2 subunit-containing nicotinic receptors mediate the enhancing effect of nicotine on trace cued fear conditioning in C57BL/6 mice. Psychopharmacology (Berl) 190(3):343-52. [PubMed: 17136517]  [MGI Ref ID J:136471]

Dziewczapolski G; Glogowski CM; Masliah E; Heinemann SF. 2009. Deletion of the alpha 7 nicotinic acetylcholine receptor gene improves cognitive deficits and synaptic pathology in a mouse model of Alzheimer's disease. J Neurosci 29(27):8805-15. [PubMed: 19587288]  [MGI Ref ID J:151080]

Egea J; Rosa AO; Sobrado M; Gandia L; Lopez MG; Garcia AG. 2007. Neuroprotection afforded by nicotine against oxygen and glucose deprivation in hippocampal slices is lost in alpha7 nicotinic receptor knockout mice. Neuroscience 145(3):866-72. [PubMed: 17291692]  [MGI Ref ID J:121645]

Fernandes C; Hoyle E; Dempster E; Schalkwyk LC; Collier DA. 2006. Performance deficit of alpha7 nicotinic receptor knockout mice in a delayed matching-to-place task suggests a mild impairment of working/episodic-like memory. Genes Brain Behav 5(6):433-40. [PubMed: 16923147]  [MGI Ref ID J:123663]

Franceschini D; Orr-Urtreger A; Yu W; Mackey LY; Bond RA; Armstrong D; Patrick JW; Beaudet AL; De Biasi M. 2000. Altered baroreflex responses in alpha7 deficient mice. Behav Brain Res 113(1-2):3-10. [PubMed: 10942027]  [MGI Ref ID J:96657]

Franceschini D; Paylor R; Broide R; Salas R; Bassetto L; Gotti C; De Biasi M. 2002. Absence of alpha7-containing neuronal nicotinic acetylcholine receptors does not prevent nicotine-induced seizures. Brain Res Mol Brain Res 98(1-2):29-40. [PubMed: 11834293]  [MGI Ref ID J:74703]

Fujii YX; Fujigaya H; Moriwaki Y; Misawa H; Kasahara T; Grando SA; Kawashima K. 2007. Enhanced serum antigen-specific IgG1 and proinflammatory cytokine production in nicotinic acetylcholine receptor alpha7 subunit gene knockout mice. J Neuroimmunol 189(1-2):69-74. [PubMed: 17675251]  [MGI Ref ID J:128969]

Grabus SD; Martin BR; Imad Damaj M. 2005. Nicotine physical dependence in the mouse: involvement of the alpha7 nicotinic receptor subtype. Eur J Pharmacol 515(1-3):90-3. [PubMed: 15896732]  [MGI Ref ID J:101876]

Grady SR ; Moretti M ; Zoli M ; Marks MJ ; Zanardi A ; Pucci L ; Clementi F ; Gotti C. 2009. Rodent habenulo-interpeduncular pathway expresses a large variety of uncommon nAChR subtypes, but only the alpha3beta4* and alpha3beta3beta4* subtypes mediate acetylcholine release. J Neurosci 29(7):2272-82. [PubMed: 19228980]  [MGI Ref ID J:146586]

Griguoli M; Cellot G; Cherubini E. 2013. In hippocampal oriens interneurons anti-Hebbian long-term potentiation requires cholinergic signaling via alpha7 nicotinic acetylcholine receptors. J Neurosci 33(3):1044-9. [PubMed: 23325242]  [MGI Ref ID J:193902]

Hellier JL; Arevalo NL; Blatner MJ; Dang AK; Clevenger AC; Adams CE; Restrepo D. 2010. Olfactory discrimination varies in mice with different levels of alpha7-nicotinic acetylcholine receptor expression. Brain Res 1358:140-50. [PubMed: 20713028]  [MGI Ref ID J:166638]

Hernandez CM; Kayed R; Zheng H; Sweatt JD; Dineley KT. 2010. Loss of alpha7 nicotinic receptors enhances beta-amyloid oligomer accumulation, exacerbating early-stage cognitive decline and septohippocampal pathology in a mouse model of Alzheimer's disease. J Neurosci 30(7):2442-53. [PubMed: 20164328]  [MGI Ref ID J:157839]

Hoyle E; Genn RF; Fernandes C; Stolerman IP. 2006. Impaired performance of alpha7 nicotinic receptor knockout mice in the five-choice serial reaction time task. Psychopharmacology (Berl) 189(2):211-23. [PubMed: 17019565]  [MGI Ref ID J:135957]

Huston JM; Ochani M; Rosas-Ballina M; Liao H; Ochani K; Pavlov VA; Gallowitsch-Puerta M; Ashok M; Czura CJ; Foxwell B; Tracey KJ; Ulloa L. 2006. Splenectomy inactivates the cholinergic antiinflammatory pathway during lethal endotoxemia and polymicrobial sepsis. J Exp Med 203(7):1623-8. [PubMed: 16785311]  [MGI Ref ID J:124406]

Jo YH; Wiedl D; Role LW. 2005. Cholinergic modulation of appetite-related synapses in mouse lateral hypothalamic slice. J Neurosci 25(48):11133-44. [PubMed: 16319313]  [MGI Ref ID J:103421]

Keller JJ; Keller AB; Bowers BJ; Wehner JM. 2005. Performance of alpha7 nicotinic receptor null mutants is impaired in appetitive learning measured in a signaled nose poke task. Behav Brain Res 162(1):143-52. [PubMed: 15922075]  [MGI Ref ID J:98842]

Kelso ML; Wehner JM; Collins AC; Scheff SW; Pauly JR. 2006. The pathophysiology of traumatic brain injury in alpha7 nicotinic cholinergic receptor knockout mice. Brain Res 1083(1):204-10. [PubMed: 16545784]  [MGI Ref ID J:108188]

Klink R; de Kerchove d'Exaerde A; Zoli M; Changeux JP. 2001. Molecular and physiological diversity of nicotinic acetylcholine receptors in the midbrain dopaminergic nuclei. J Neurosci 21(5):1452-63. [PubMed: 11222635]  [MGI Ref ID J:109365]

Koval LM; Zverkova AS; Grailhe R; Utkin YN; Tsetlin VI; Komisarenko SV; Skok MV. 2008. Nicotinic acetylcholine receptors alpha4beta2 and alpha7 regulate myelo- and erythropoiesis within the bone marrow. Int J Biochem Cell Biol 40(5):980-90. [PubMed: 18083057]  [MGI Ref ID J:140280]

Laudenbach V; Medja F; Zoli M; Rossi FM; Evrard P; Changeux JP; Gressens P. 2002. Selective activation of central subtypes of the nicotinic acetylcholine receptor has opposite effects on neonatal excitotoxic brain injuries. FASEB J 16(3):423-5. [PubMed: 11790724]  [MGI Ref ID J:120462]

Levin ED; Petro A; Rezvani AH; Pollard N; Christopher NC; Strauss M; Avery J; Nicholson J; Rose JE. 2009. Nicotinic alpha7- or beta2-containing receptor knockout: effects on radial-arm maze learning and long-term nicotine consumption in mice. Behav Brain Res 196(2):207-13. [PubMed: 18831991]  [MGI Ref ID J:148632]

Liu Z; Neff RA; Berg DK. 2006. Sequential interplay of nicotinic and GABAergic signaling guides neuronal development. Science 314(5805):1610-3. [PubMed: 17158331]  [MGI Ref ID J:116224]

Lozada AF; Wang X; Gounko NV; Massey KA; Duan J; Liu Z; Berg DK. 2012. Glutamatergic Synapse Formation is Promoted by alpha7-Containing Nicotinic Acetylcholine Receptors. J Neurosci 32(22):7651-61. [PubMed: 22649244]  [MGI Ref ID J:185201]

Lozada AF; Wang X; Gounko NV; Massey KA; Duan J; Liu Z; Berg DK. 2012. Induction of Dendritic Spines by beta2-Containing Nicotinic Receptors. J Neurosci 32(24):8391-400. [PubMed: 22699919]  [MGI Ref ID J:185573]

Mameli-Engvall M; Evrard A; Pons S; Maskos U; Svensson TH; Changeux JP; Faure P. 2006. Hierarchical control of dopamine neuron-firing patterns by nicotinic receptors. Neuron 50(6):911-21. [PubMed: 16772172]  [MGI Ref ID J:122939]

Maouche K; Polette M; Jolly T; Medjber K; Cloez-Tayarani I; Changeux JP; Burlet H; Terryn C; Coraux C; Zahm JM; Birembaut P; Tournier JM. 2009. {alpha}7 nicotinic acetylcholine receptor regulates airway epithelium differentiation by controlling basal cell proliferation. Am J Pathol 175(5):1868-82. [PubMed: 19808646]  [MGI Ref ID J:154705]

Marubio LM; Paylor R. 2004. Impaired passive avoidance learning in mice lacking central neuronal nicotinic acetylcholine receptors. Neuroscience 129(3):575-82. [PubMed: 15541879]  [MGI Ref ID J:94667]

Morley BJ; Rodriguez-Sierra JF. 2004. A phenotype for the alpha7 nicotinic acetylcholine receptor null mutant. Brain Res 1023(1):41-7. [PubMed: 15364017]  [MGI Ref ID J:92540]

Nakauchi S; Sumikawa K. 2012. Endogenously released ACh and exogenous nicotine differentially facilitate long-term potentiation induction in the hippocampal CA1 region of mice. Eur J Neurosci 35(9):1381-95. [PubMed: 22462479]  [MGI Ref ID J:191620]

Naylor C; Quarta D; Fernandes C; Stolerman IP. 2005. Tolerance to nicotine in mice lacking alpha7 nicotinic receptors. Psychopharmacology (Berl) 180(3):558-63. [PubMed: 15723228]  [MGI Ref ID J:114294]

Nicolussi EM; Huck S; Lassmann H; Bradl M. 2009. The cholinergic anti-inflammatory system limits T cell infiltration into the neurodegenerative CNS, but cannot counteract complex CNS inflammation. Neurobiol Dis 35(1):24-31. [PubMed: 19344760]  [MGI Ref ID J:150473]

Nizri E; Irony-Tur-Sinai M; Lory O; Orr-Urtreger A; Lavi E; Brenner T. 2009. Activation of the cholinergic anti-inflammatory system by nicotine attenuates neuroinflammation via suppression of Th1 and Th17 responses. J Immunol 183(10):6681-8. [PubMed: 19846875]  [MGI Ref ID J:157180]

Osborne-Hereford AV; Rogers SW; Gahring LC. 2008. Neuronal nicotinic alpha7 receptors modulate inflammatory cytokine production in the skin following ultraviolet radiation. J Neuroimmunol 193(1-2):130-9. [PubMed: 18077004]  [MGI Ref ID J:130590]

Paylor R; Nguyen M; Crawley JN; Patrick J; Beaudet A; Orr-Urtreger A. 1998. Alpha7 nicotinic receptor subunits are not necessary for hippocampal-dependent learning or sensorimotor gating: a behavioral characterization of Acra7-deficient mice. Learn Mem 5(4-5):302-16. [PubMed: 10454356]  [MGI Ref ID J:51149]

Pena G; Cai B; Liu J; van der Zanden EP; Deitch EA; de Jonge WJ; Ulloa L. 2010. Unphosphorylated STAT3 modulates alpha 7 nicotinic receptor signaling and cytokine production in sepsis. Eur J Immunol 40(9):2580-9. [PubMed: 20706987]  [MGI Ref ID J:165922]

Portugal GS; Kenney JW; Gould TJ. 2008. beta2 subunit containing acetylcholine receptors mediate nicotine withdrawal deficits in the acquisition of contextual fear conditioning. Neurobiol Learn Mem 89(2):106-13. [PubMed: 17584502]  [MGI Ref ID J:129307]

Rabenstein RL; Caldarone BJ; Picciotto MR. 2006. The nicotinic antagonist mecamylamine has antidepressant-like effects in wild-type but not beta2- or alpha7-nicotinic acetylcholine receptor subunit knockout mice. Psychopharmacology (Berl) 189(3):395-401. [PubMed: 17016705]  [MGI Ref ID J:136482]

Sack R; Gochberg-Sarver A; Rozovsky U; Kedmi M; Rosner S; Orr-Urtreger A. 2005. Lower core body temperature and attenuated nicotine-induced hypothermic response in mice lacking the beta4 neuronal nicotinic acetylcholine receptor subunit. Brain Res Bull 66(1):30-6. [PubMed: 15925141]  [MGI Ref ID J:101835]

Salas R; Main A; Gangitano D; De Biasi M. 2007. Decreased withdrawal symptoms but normal tolerance to nicotine in mice null for the alpha7 nicotinic acetylcholine receptor subunit. Neuropharmacology 53(7):863-9. [PubMed: 17920082]  [MGI Ref ID J:141484]

Skok M; Grailhe R; Agenes F; Changeux JP. 2006. The role of nicotinic acetylcholine receptors in lymphocyte development. J Neuroimmunol 171(1-2):86-98. [PubMed: 16253349]  [MGI Ref ID J:112766]

Skok M; Grailhe R; Changeux JP. 2005. Nicotinic receptors regulate B lymphocyte activation and immune response. Eur J Pharmacol 517(3):246-51. [PubMed: 15963492]  [MGI Ref ID J:103908]

Stolerman IP; Chamberlain S; Bizarro L; Fernandes C; Schalkwyk L. 2004. The role of nicotinic receptor alpha 7 subunits in nicotine discrimination. Neuropharmacology 46(3):363-71. [PubMed: 14975691]  [MGI Ref ID J:97002]

Su X; Lee JW; Matthay ZA; Mednick G; Uchida T; Fang X; Gupta N; Matthay MA. 2007. Activation of the alpha7 nAChR reduces acid-induced acute lung injury in mice and rats. Am J Respir Cell Mol Biol 37(2):186-92. [PubMed: 17431097]  [MGI Ref ID J:138495]

Su X; Matthay MA; Malik AB. 2010. Requisite role of the cholinergic alpha7 nicotinic acetylcholine receptor pathway in suppressing Gram-negative sepsis-induced acute lung inflammatory injury. J Immunol 184(1):401-10. [PubMed: 19949071]  [MGI Ref ID J:159042]

Sydserff S; Sutton EJ; Song D; Quirk MC; Maciag C; Li C; Jonak G; Gurley D; Gordon JC; Christian EP; Doherty JJ; Hudzik T; Johnson E; Mrzljak L; Piser T; Smagin GN; Wang Y; Widzowski D; Smith JS. 2009. Selective alpha7 nicotinic receptor activation by AZD0328 enhances cortical dopamine release and improves learning and attentional processes. Biochem Pharmacol 78(7):880-8. [PubMed: 19615981]  [MGI Ref ID J:154876]

Vida G; Pena G; Deitch EA; Ulloa L. 2011. {alpha}7-Cholinergic Receptor Mediates Vagal Induction of Splenic Norepinephrine. J Immunol 186(7):4340-6. [PubMed: 21339364]  [MGI Ref ID J:170845]

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Wang X; Yang Z; Xue B; Shi H. 2011. Activation of the cholinergic antiinflammatory pathway ameliorates obesity-induced inflammation and insulin resistance. Endocrinology 152(3):836-46. [PubMed: 21239433]  [MGI Ref ID J:173882]

Wehner JM; Keller JJ; Keller AB; Picciotto MR; Paylor R; Booker TK; Beaudet A; Heinemann SF; Balogh SA. 2004. Role of neuronal nicotinic receptors in the effects of nicotine and ethanol on contextual fear conditioning. Neuroscience 129(1):11-24. [PubMed: 15489024]  [MGI Ref ID J:94231]

Wilund KR; Rosenblat M; Chung HR; Volkova N; Kaplan M; Woods JA; Aviram M. 2009. Macrophages from alpha 7 nicotinic acetylcholine receptor knockout mice demonstrate increased cholesterol accumulation and decreased cellular paraoxonase expression: a possible link between the nervous system and atherosclerosis development. Biochem Biophys Res Commun 390(1):148-54. [PubMed: 19785985]  [MGI Ref ID J:155229]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred to heterozygous mice. Although homozygotes are viable and fertile, the donating investigator reports that breeding is better using heterozygous breeders, and they have observed reduced fertility in homozygotes. Heterozygotes produce small litters. Expected coat color from breeding is Black.
Mating System	Heterozygote x Homozygote         (Female x Male)   27-APR-10
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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phone:	207-288-6470
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JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.