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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Major Histocompatibility Congenic; Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N?+2 Donating Investigator Stephen Hedrick, University of California at San Diego Description
PCC transgenic mice are viable and fertile. Pigeon cytochrome c was engineered for expression in lymphoid tissues of transgenic mice in two forms: one in which it is expressed as a type II plasma membrane protein (mPCC), and a second in which it was targeted to the mitochondria after cytoplasmic synthesis (ePCC). This strain carries the mPCC construct. (For mice carrying the ePCC construct, see strain 003221.) PCC is expressed in thymus, spleen and LPS stimulated B cells in both strains of mice. mRNA expression levels are higher in mPCC mice than in ePCC mice. However, tolerance is induced in the thymus of both strains, regardless of antigen compartmentation.
Related Strains
Strains carrying H2a allele
000469 B10.A-H2a H2-T18a/SgSnJ View Strains carrying H2a (1 strain)
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
H2a relatedApoptosis Research
Endogenous Regulators
Developmental Biology Research
Embryonic Lethality (Homozygous)
Postnatal Mortality (Homozygous)
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Genes & Alleles
Gene & Allele Information
| Allele Symbol | H2a | ||
|---|---|---|---|
| Allele Name | a variant | ||
| Allele Type | Not Applicable | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| General Note | The a variant has been observed in the following strains: A/J, B10.A | ||
| Allele Symbol | Tg(H2KmPCC)2939Stoe | ||
| Allele Name | transgene insertion 2939, Stephan Oehan | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Stephan Oehen, Cytos Biotechnology AG | ||
| Strain of Origin | C57BL/6 x SJL | ||
| Expressed Gene | PCC, Cataract, polymorphic congenital, autosomal dominant nonnuclear, human | ||
| General Note | Transgenic mice are viable and fertile. PCC is expressed in thymus, spleen and LPS stimulated B cells; and tolerance is induced in the thymus. | ||
| Molecular Note | Pigeon cytochrome c was engineered for expression in lymphoid tissues of transgenic mice in two forms. The form of cytochrome c in this construct is expressed as a type II plasma membrane protein (mPCC). mRNA expression levels are higher in mice carryingthis transgene than in mice carrying Tg(H2KePCC)2403. [MGI Ref ID J:92997] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Oehen S; Feng L; Xia Y; Surh CD; Hedrick SM. 1996. Antigen compartmentation and T helper cell tolerance induction. J Exp Med 183(6):2617-26. [PubMed: 8676082] [MGI Ref ID J:92997]
Additional References
H2a relatedTg(H2KmPCC)2939Stoe relatedCemerski S; Das J; Locasale J; Arnold P; Giurisato E; Markiewicz MA; Fremont D; Allen PM; Chakraborty AK; Shaw AS. 2007. The stimulatory potency of T cell antigens is influenced by the formation of the immunological synapse. Immunity 26(3):345-55. [PubMed: 17346997] [MGI Ref ID J:120073]
El-Sawy T; Belperio JA; Strieter RM; Remick DG; Fairchild RL. 2005. Inhibition of polymorphonuclear leukocyte-mediated graft damage synergizes with short-term costimulatory blockade to prevent cardiac allograft rejection. Circulation 112(3):320-31. [PubMed: 15998678] [MGI Ref ID J:117219]
Klein J; Figueroa F; David CS. 1983. H-2 haplotypes, genes and antigens: second listing. II. The H-2 complex. Immunogenetics 17(6):553-96. [PubMed: 6407984] [MGI Ref ID J:7097]
Miyashita N; Migita S; Moriwaki K. 1987. Effects of H-2 complex and non-H-2 background on urethane-induced chromosomal aberrations in mice. Mutat Res 176(1):59-67. [PubMed: 3099189] [MGI Ref ID J:109945]
Peterson KE; Iwashiro M; Hasenkrug KJ; Chesebro B. 2000. Major histocompatibility complex class I gene controls the generation of gamma interferon-producing CD4(+) and CD8(+) T cells important for recovery from friend retrovirus-induced leukemia J Virol 74(11):5363-7. [PubMed: 10799615] [MGI Ref ID J:62492]
Bouzahzah F; Jung S; Craft J. 2003. CD4+ T cells from lupus-prone mice avoid antigen-specific tolerance induction in vivo. J Immunol 170(2):741-8. [PubMed: 12517936] [MGI Ref ID J:133249]
Huang CY; Golub R; Wu GE; Kanagawa O. 2002. Superantigen-induced TCR alpha locus secondary rearrangement: role in tolerance induction. J Immunol 168(7):3259-65. [PubMed: 11907080] [MGI Ref ID J:126392]
Tanchot C; Barber DL; Chiodetti L; Schwartz RH. 2001. Adaptive tolerance of CD4+ T cells in vivo: multiple thresholds in response to a constant level of antigen presentation. J Immunol 167(4):2030-9. [PubMed: 11489985] [MGI Ref ID J:120226]
Wang F; Huang CY; Kanagawa O. 1998. Rapid deletion of rearranged T cell antigen receptor (TCR) Valpha-Jalpha segment by secondary rearrangement in the thymus: role of continuous rearrangement of TCR alpha chain gene and positive selection in the T cell repertoire formation. Proc Natl Acad Sci U S A 95(20):11834-9. [PubMed: 9751751] [MGI Ref ID J:50143]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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General Terms and Conditions
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