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General Terms and Conditions

Former Name	Tnfr2    (Changed: 15-DEC-04 )
Genes & Alleles	Tnfrsf1b;   Tnfrsf1btm1Imx;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Background Strain C57BL/6 Donor Strain 129S7 via AB1 ES cell line (+Hprt-bm2) Donating Investigator Jacques Peschon,   Amgen Generation N5F?+5p (30-MAY-04)

Strain Description
Mice homozygous for a Tnfrsf1b^tm1Imx targeted mutation (formerly Tnfr2^tm1Imx, p75 deficient) are viable and fertile. T cells from homozygous mutant mice display defects in activation induced cell death in vitro and dramatically elevated levels of circulating Tnf following systemic challenge with LPS. Pulmonary neutrophil influx is exacerbated in p75 deficient mice in a model of hypersensitivity pneumonitis.

Strain Development
The p75 mutation was made by homologous recombination in the 129/SvEv derived AB1 ES cell line using a gene replacement vector in which a 1 kb Kas1-BamH1 fragment was replaced with a PGKneo cassette. The resulting null mutation results in a deletion of amino acids 3 - 26. This strain was generated on a 129/SvEv background and is currently at N5 on a C57BL/6 background.

Related Disease (OMIM) Terms NOT Hyperlipidemia, Familial Combined; FCHL NOT Hypertension, Essential

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Tnfrsf1btm1Imx/Tnfrsf1btm1Imx         involves: 129S7/SvEvBrd * C57BL/6 immune system phenotype increased circulating tumor necrosis factor level (MGI Ref ID J:45147) two hours post i.v. lipopolysaccharide (LPS) challenge serum levels are 5 fold higher than controls, however, TNF kinetics and activity are not altered increased inflammatory response (MGI Ref ID J:45147) increased pulmonary neutrophil accumulation in response to intranasal instillation of M. faeni as compared to control, however monocyte and lymphocyte levels are comparable to control homeostasis/metabolism phenotype increased circulating tumor necrosis factor level (MGI Ref ID J:45147) two hours post i.v. lipopolysaccharide (LPS) challenge serum levels are 5 fold higher than controls, however, TNF kinetics and activity are not altered

Gene & Allele Details

Allele Symbol		Tnfrsf1btm1Imx
Allele Name		targeted mutation 1, Immunex Research and Development Corporation
Common Name(s)		TNFR2-; TNFRII-; TNFbeta ko; Tnfrsf1btm1Imx; p75 KO; p75-;
Mutation Made By		Jacques Peschon,   Amgen
Strain of Origin		129S7/SvEvBrd-Hprt1<+>
ES Cell Line Name		AB1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name		Tnfrsf1b, tumor necrosis factor receptor superfamily, member 1b
Chromosome		4
Gene Common Name(s)		CD120b; TBPII; TNF-R-II; TNF-R2; TNF-R75; TNF-alphaR2; TNFBR; TNFR1B; TNFR2; TNFR80; TNFRII; Tnfr-1; Tnfr2; p75; p75 TNFR; p75TNFR; tumor necrosis factor receptor 1; tumor necrosis factor receptor 2;
Molecular Note		A PGK-neomycin resistance cassette replaced 1kb of sequence encoding amino acids 3 - 26. Lack of gene product was shown functionally. [MGI Ref ID J:45147]

Control Information

Allele	Control
Tnfrsf1btm1Imx	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Tnfrsf1b^tm1Imx

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Related Strains

Strains carrying   Tnfrsf1b^tm1Imx allele

003243

B6;129S-Tnfrsf1atm1Imx Tnfrsf1btm1Imx/J

View Strains carrying   Tnfrsf1b^tm1Imx     (1 strain)

Strains carrying other alleles of Tnfrsf1b

002620

B6.129S2-Tnfrsf1btm1Mwm/J

View Strains carrying other alleles of Tnfrsf1b     (1 strain)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Tnfrsf1b^tm1Imx related

Apoptosis Research
Death Receptors

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Inflammation

References

Selected Reference(s)

Peschon JJ; Torrance DS; Stocking KL; Glaccum MB; Otten C; Willis CR ; Charrier K ; Morrissey PJ ; Ware CB ; Mohler KM. 1998. TNF receptor-deficient mice reveal divergent roles for p55 and p75 in several models of inflammation. J Immunol 160(2):943-52. [PubMed: 9551933]  [MGI Ref ID J:45147]

Additional References

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Price and Supply Information

Strain Name:	B6.129S7-Tnfrsf1btm1Imx/J
Stock Number:	003246

Price Details

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Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below
Control Information	View Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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