Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N6+p

Description
Kitl^Sl-20J is a dominant allele. On the C57BL/6J background heterozygotes have a light black coat color with lighter tail and feet and a steel colored belly with a belly spot. Heterozygotes of both sexes are viable and fertile.

Related Strains

Strains carrying other alleles of Kitl

000090	129S1/Sv-Oca2+ Tyr+ KitlSl-J/J
002993	B6.Cg-KitlSl-18H/EiJ
008656	B6.Cg-KitlSl-gb/MbeJ
000124	B6.Cg-KitlSl Krt71Ca/J
000160	B6.D2-KitlSl-d/J
000291	C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J
001380	C3Sn.Cg-KitlSl-con/J
000979	STOCK KitlSl-16J/J
000161	WB.D2-KitlSl-d/J
000693	WC/ReJ KitlSl/J
100401	WCB6F1/J KitlSl KitlSl-d

View Strains carrying other alleles of Kitl     (11 strains)

Additional Web Information

JAX^® NOTES, February 2001; 481. Mgf Gene Name Changes to Kitl.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Kitl^Sl-20J/Kitl⁺

        C57BL/6J

• pigmentation phenotype
• belly spot (MGI Ref ID J:86436)
  • heterozygous mice have either a white belly spot or dilute ventral pigmentation
• skin/coat/nails phenotype
• belly spot (MGI Ref ID J:86436)
  • heterozygous mice have either a white belly spot or dilute ventral pigmentation

Kitl^Sl-20J/Kitl^Sl-20J

        C57BL/6J

• lethality-prenatal/perinatal
• lethality throughout fetal growth and development (MGI Ref ID J:86436)
  • no homozygous mice are born
  • normal ratio of genotypes at E14.5
• growth/size phenotype
• decreased fetal size (MGI Ref ID J:86436)
  • homozygous embryos were runted at E14.5
• hematopoietic system phenotype
• abnormal erythropoiesis (MGI Ref ID J:86436)
  • reduced CFU-E/BFU-E ratio indicating reduced erythroid progenitors during terminal differentiation
• reproductive system phenotype
• absent primordial germ cells (MGI Ref ID J:86436)
  • no primordial germ cells present at E13.5
• liver/biliary system phenotype
• pale liver (MGI Ref ID J:86436)
• skin/coat/nails phenotype
• pallor (MGI Ref ID J:86436)
  • fetus pale at E14.5

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Kitl^Sl-20J related

Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence
      Gonadal Tumors: ovarian and testicular

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Crest Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Hypothalamus/Pituitary Defects
Skin Defects

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency
      Mast Cell Deficiency

Neurobiology Research
Vestibular and Hearing Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient
Fertility Defects
Gonadal Tumors
      ovarian and testicular

Research Tools
Immunology and Inflammation Research
      Mast Cell Deficiency

Sensorineural Research
Vestibular and Hearing Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		KitlSl-20J
Allele Name		steel 20 Jackson
Allele Type		Spontaneous
Strain of Origin		C57BL/6J
Gene Symbol and Name		Kitl, kit ligand
Chromosome		10
Gene Common Name(s)		Clo; Con; DKFZp686F2250; FPH2; Gb; KL-1; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor;
General Note		This spontaneous mutation arose in a C57BL/6J breeding colony at The Jackson Laboratory.
Molecular Note		Long-range PCR demonstrated that this mutation is an in-frame tandem duplication of exon 3 which also involves duplication of introns 3 and 2. At the junction between intron 3 and intron 2 there is an inverted 141 bp duplication of a sequence found later in intron 2. The duplication of exon 3 results in a loss of function in the expressed protein due either to defective binding to its receptor or failure to activate the receptor once bound. [MGI Ref ID J:86436]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

Kitl^Sl-20J related

Chandra S; Kapur R; Chuzhanova N; Summey V; Prentice D; Barker J; Cooper DN; Williams DA. 2003. A rare complex DNA rearrangement in the murine Steel gene results in exon duplication and a lethal phenotype. Blood 102(10):3548-55. [PubMed: 12881302]  [MGI Ref ID J:86436]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). Genomic DNA is available for this strain from the Mouse DNA Resource.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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