Strain Name:

STOCK Igf1tm1Ts/ImJ

Stock Number:

003258

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Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names IGF-1 Mini    (Changed: 15-DEC-04 )
STOCK Igf1tm1Ts/Im/J    (Changed: 15-DEC-04 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Timothy Stewart,  

Description
The majority of mice homozygous for the Igf1tm1Tstargeted mutation die perinatally. Only ~4% of homozygotes survive beyond birth for up to 4 months of age. Heterozygotes have serum levels of IGF-1 that are 37% lower than in wild type mice. Body size is, proportionally, 10-20% smaller than that of normal wild type siblings, with no apparent histological abnormalities.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Igf1
016831   B6.129(FVB)-Igf1tm1Dlr/J
012663   FVB.129(B6)-Igf1tm1Dlr/J
003259   STOCK Igf1tm2Ts/ImJ
View Strains carrying other alleles of Igf1     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Insulin-Like Growth Factor I Deficiency   (IGF1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Igf1tm1Ts/Igf1+

        involves: 129S7/SvEvBrd * C57BL/6J
  • growth/size/body phenotype
  • abnormal postnatal growth/weight/body size   (MGI Ref ID J:16214)

Igf1tm1Ts/Igf1tm1Ts

        involves: 129S7/SvEvBrd * C57BL/6J
  • mortality/aging
  • perinatal lethality   (MGI Ref ID J:16214)
  • growth/size/body phenotype
  • abnormal embryonic growth/weight/body size   (MGI Ref ID J:16214)
  • muscle phenotype
  • abnormal muscle morphology   (MGI Ref ID J:16214)
  • respiratory system phenotype
  • abnormal respiratory system morphology   (MGI Ref ID J:16214)
  • abnormal respiratory system physiology   (MGI Ref ID J:16214)
  • nervous system phenotype
  • abnormal brain morphology   (MGI Ref ID J:24953)
  • abnormal nervous system physiology   (MGI Ref ID J:24953)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Igf1tm1Ts related

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines

Endocrine Deficiency Research
Mammary Gland Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Igf1tm1Ts
Allele Name targeted mutation 1, Timothy Stewart
Allele Type Targeted (knock-out)
Common Name(s) IGF-I-;
Mutation Made ByDr. Timothy Stewart,  
Strain of Origin129S7/SvEvBrd-Hprt<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name Igf1, insulin-like growth factor 1
Chromosome 10
Gene Common Name(s) C730016P09Rik; IGF-I; IGF1A; IGFI; Igf-1; RIKEN cDNA C730016P09 gene;
Molecular Note A neomycin selection cassette was inserted into exon 3 after codon 15. This mutation inserts multiple stop codons into all reading frames of the B chain of the encoded protein. RT-PCR analysis demonstrated that the transcript is undetectable in homozygous animals. [MGI Ref ID J:16214]

Genotyping

Genotyping Information

Genotyping Protocols

Igf1tm1Ts, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Powell-Braxton L; Hollingshead P; Warburton C; Dowd M; Pitts-Meek S; Dalton D; Gillett N; Stewart TA. 1993. IGF-I is required for normal embryonic growth in mice. Genes Dev 7(12B):2609-17. [PubMed: 8276243]  [MGI Ref ID J:16214]

Additional References

Beck KD; Powell-Braxton L; Widmer HR; Valverde J; Hefti F. 1995. Igf1 gene disruption results in reduced brain size, CNS hypomyelination, and loss of hippocampal granule and striatal parvalbumin-containing neurons. Neuron 14(4):717-30. [PubMed: 7718235]  [MGI Ref ID J:24953]

Igf1tm1Ts related

Beck KD; Powell-Braxton L; Widmer HR; Valverde J; Hefti F. 1995. Igf1 gene disruption results in reduced brain size, CNS hypomyelination, and loss of hippocampal granule and striatal parvalbumin-containing neurons. Neuron 14(4):717-30. [PubMed: 7718235]  [MGI Ref ID J:24953]

Bikle D; Majumdar S; Laib A; Powell-Braxton L; Rosen C; Beamer W; Nauman E; Leary C; Halloran B. 2001. The skeletal structure of insulin-like growth factor I-deficient mice. J Bone Miner Res 16(12):2320-9. [PubMed: 11760848]  [MGI Ref ID J:112522]

Burghardt AJ; Wang Y; Elalieh H; Thibault X; Bikle D; Peyrin F; Majumdar S. 2007. Evaluation of fetal bone structure and mineralization in IGF-I deficient mice using synchrotron radiation microtomography and Fourier transform infrared spectroscopy. Bone 40(1):160-8. [PubMed: 16905376]  [MGI Ref ID J:141513]

Cheng CM; Mervis RF; Niu SL; Salem N Jr; Witters LA; Tseng V; Reinhardt R; Bondy CA. 2003. Insulin-like growth factor 1 is essential for normal dendritic growth. J Neurosci Res 73(1):1-9. [PubMed: 12815703]  [MGI Ref ID J:104820]

Cheng CM; Reinhardt RR; Lee WH; Joncas G; Patel SC; Bondy CA. 2000. Insulin-like growth factor 1 regulates developing brain glucose metabolism. Proc Natl Acad Sci U S A 97(18):10236-41. [PubMed: 10954733]  [MGI Ref ID J:125044]

Cheng CM; Tseng V; Wang J; Wang D; Matyakhina L; Bondy CA. 2005. Tau is hyperphosphorylated in the insulin-like growth factor-I null brain. Endocrinology 146(12):5086-91. [PubMed: 16123158]  [MGI Ref ID J:129785]

Dube PE; Forse CL; Bahrami J; Brubaker PL. 2006. The essential role of insulin-like growth factor-1 in the intestinal tropic effects of glucagon-like peptide-2 in mice. Gastroenterology 131(2):589-605. [PubMed: 16890611]  [MGI Ref ID J:124914]

Fournier M; Lewis MI. 2000. Influences of IGF-I gene disruption on the cellular profile of the diaphragm. Am J Physiol Endocrinol Metab 278(4):E707-15. [PubMed: 10751206]  [MGI Ref ID J:62401]

Gao WQ; Shinsky N; Ingle G; Beck K; Elias KA; Powell-Braxton L. 1999. IGF-I deficient mice show reduced peripheral nerve conduction velocities and decreased axonal diameters and respond to exogenous IGF-I treatment. J Neurobiol 39(1):142-52. [PubMed: 10213459]  [MGI Ref ID J:54415]

He J; Rosen CJ; Adams DJ; Kream BE. 2006. Postnatal growth and bone mass in mice with IGF-I haploinsufficiency. Bone 38(6):826-35. [PubMed: 16427371]  [MGI Ref ID J:111150]

Kiryakova S; Sohnchen J; Grosheva M; Schuetz U; Marinova Ts; Dzhupanova R; Sinis N; Hubbers CU; Skouras E; Ankerne J; Fries JW; Irintchev A; Dunlop SA; Angelov DN. 2010. Recovery of whisking function promoted by manual stimulation of the vibrissal muscles after facial nerve injury requires insulin-like growth factor 1 (IGF-1). Exp Neurol 222(2):226-34. [PubMed: 20067789]  [MGI Ref ID J:158838]

Kleinberg DL; Ruan W; Yee D; Kovacs KT; Vidal S. 2007. Insulin-like growth factor (IGF)-I controls prostate fibromuscular development: IGF-I inhibition prevents both fibromuscular and glandular development in eugonadal mice. Endocrinology 148(3):1080-8. [PubMed: 17138659]  [MGI Ref ID J:129595]

Moerth C; Schneider MR; Renner-Mueller I; Blutke A; Elmlinger MW; Erben RG; Camacho-Hubner C; Hoeflich A; Wolf E. 2007. Postnatally elevated levels of insulin-like growth factor (IGF)-II fail to rescue the dwarfism of IGF-I-deficient mice except kidney weight. Endocrinology 148(1):441-51. [PubMed: 17008389]  [MGI Ref ID J:119210]

Niu T; Rosen CJ. 2005. The insulin-like growth factor-I gene and osteoporosis: a critical appraisal. Gene 361:38-56. [PubMed: 16183214]  [MGI Ref ID J:102683]

Rogers SA; Powell-Braxton L; Hammerman MR. 1999. Insulin-like growth factor I regulates renal development in rodents. Dev Genet 24(3-4):293-8. [PubMed: 10322637]  [MGI Ref ID J:54491]

Ruan W; Kleinberg DL. 1999. Insulin-like growth factor I is essential for terminal end bud formation and ductal morphogenesis during mammary development. Endocrinology 140(11):5075-81. [PubMed: 10537134]  [MGI Ref ID J:58345]

Ruan W; Powell-Braxton L; Kopchick JJ; Kleinberg DL. 1999. Evidence that insulin-like growth factor I and growth hormone are required for prostate gland development. Endocrinology 140(5):1984-9. [PubMed: 10218945]  [MGI Ref ID J:54846]

Stefaneanu L; Powell-Braxton L; Won W; Chandrashekar V; Bartke A. 1999. Somatotroph and lactotroph changes in the adenohypophyses of mice with disrupted insulin-like growth factor I gene. Endocrinology 140(9):3881-9. [PubMed: 10465256]  [MGI Ref ID J:108805]

Wang J; Zhou J; Bondy CA. 1999. Igf1 promotes longitudinal bone growth by insulin-like actions augmenting chondrocyte hypertrophy. FASEB J 13(14):1985-90. [PubMed: 10544181]  [MGI Ref ID J:58350]

Wang J; Zhou J; Cheng CM; Kopchick JJ; Bondy CA. 2004. Evidence supporting dual, IGF-I-independent and IGF-I-dependent, roles for GH in promoting longitudinal bone growth. J Endocrinol 180(2):247-55. [PubMed: 14765976]  [MGI Ref ID J:87987]

Wang J; Zhou J; Powell-Braxton L; Bondy C. 1999. Effects of Igf1 gene deletion on postnatal growth patterns. Endocrinology 140(7):3391-4. [PubMed: 10385438]  [MGI Ref ID J:56188]

Wang Y; Nishida S; Elalieh HZ; Long RK; Halloran BP; Bikle DD. 2006. Role of IGF-I signaling in regulating osteoclastogenesis. J Bone Miner Res 21(9):1350-8. [PubMed: 16939393]  [MGI Ref ID J:128079]

Wang Y; Nishida S; Sakata T; Elalieh HZ; Chang W; Halloran BP; Doty SB; Bikle DD. 2006. Insulin-like growth factor-I is essential for embryonic bone development. Endocrinology 147(10):4753-61. [PubMed: 16857753]  [MGI Ref ID J:113656]

Warburton C; Powell-Braxton L. 1995. Mouse models of IGF-I deficiency generated by gene targeting. Receptor 5(1):35-41. [PubMed: 7613482]  [MGI Ref ID J:26655]

Ye P; Li L; Richards RG; DiAugustine RP; D'Ercole AJ. 2002. Myelination is altered in insulin-like growth factor-I null mutant mice. J Neurosci 22(14):6041-51. [PubMed: 12122065]  [MGI Ref ID J:77994]

Zhou J; Bievre M; Bondy CA. 2000. Reduced GLUT1 expression in Igf1-/- null oocytes and follicles. Growth Horm IGF Res 10(3):111-7. [PubMed: 10942631]  [MGI Ref ID J:102609]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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