Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names STOCK Igf1tm2Ts/Im/J    (Changed: 10-JAN-05 ) IGF-1 Midi    (Changed: 15-DEC-04 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Timothy Stewart,   no forwarding info

Description
Mice homozygous for the Igf1^tm2Ts mutation (also referred to as the Igf1^m/m midi targeted insertion) are viable and fertile, but are small in size, reaching only approximately 60 - 65% the weight of their wild type siblings. Serum levels of IGF-1 in these mutants is 30% of wild type values. They have no gross histological abnormalities. Arterial blood pressure is higher in Igf1^m/m mice than in wild type controls

Related Strains

Strains carrying other alleles of Igf1

016831	B6.129(FVB)-Igf1tm1Dlr/J
012663	FVB.129(B6)-Igf1tm1Dlr/J
003258	STOCK Igf1tm1Ts/ImJ

View Strains carrying other alleles of Igf1     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Igf1^tm2Ts/Igf1^tm2Ts

        involves: 129S7/SvEvBrd * C57BL/6J

• growth/size phenotype
• decreased body weight
  • 36% reduction in adult body weight   (MGI Ref ID J:37969)
  • spleen, thymus, and heart are reduced proportionately to body weight, while brain, kidneys, liver and tibial length are less affected   (MGI Ref ID J:37969)
• cardiovascular system phenotype
• increased systemic arterial blood pressure
  • elevation of mean arterial pressure, however heart rate is normal   (MGI Ref ID J:37969)
• • increased systemic arterial diastolic blood pressure   (MGI Ref ID J:37969)
  • increased systemic arterial systolic blood pressure
    • increase in systolic pressure attains only borderline statistical significance   (MGI Ref ID J:37969)
• increased ventricle muscle contractility
  • measurements of left ventricular contractility are increased, however the hypertrophic response of the left ventricular myocardium in response to aortic constriction is preserved   (MGI Ref ID J:37969)
• homeostasis/metabolism phenotype
• decreased circulating glucose level
  • serum glucose is significantly lower in young adults, however insulin resistance is not present at this age   (MGI Ref ID J:37969)
• decreased circulating growth hormone level   (MGI Ref ID J:37969)
• muscle phenotype
• increased ventricle muscle contractility
  • measurements of left ventricular contractility are increased, however the hypertrophic response of the left ventricular myocardium in response to aortic constriction is preserved   (MGI Ref ID J:37969)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Igf1^tm2Ts related

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines

Endocrine Deficiency Research
Mammary Gland Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Igf1tm2Ts
Allele Name		targeted mutation 2, Timothy Stewart
Allele Type		Targeted (knock-out)
Common Name(s)		IGF-Im; Midi allele;
Mutation Made By		Timothy Stewart,   no forwarding info
Strain of Origin		129S7/SvEvBrd-Hprt<+>
ES Cell Line Name		AB1
ES Cell Line Strain		129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name		Igf1, insulin-like growth factor 1
Chromosome		10
Gene Common Name(s)		C730016P09Rik; IGF-I; IGF1A; IGFI; Igf-1; RIKEN cDNA C730016P09 gene;
Molecular Note		Hypomorph. In this allele, a site specific insertion of the entire targeting vector occurred instead of homologous recombination. The construct interrupted exon 3 with a neomycin cassette, with a thymidine kinase gene and vector sequences inserted 5' of the endogenous exon 3. RT-PCR analysis demonstrated that a reduced amount of normal transcript was produced from this allele as well as a mutant transcript, and a low, but detectable level of the protein was present in sera of homozygous mice. [MGI Ref ID J:37969]

Genotyping

Genotyping Information

Genotyping Protocols

NEOTD (Generic Neo), Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lembo G; Rockman HA; Hunter JJ; Steinmetz H; Koch WJ; Ma L; Prinz MP; Ross J Jr; Chien KR; Powell-Braxton L. 1996. Elevated blood pressure and enhanced myocardial contractility in mice with severe IGF-1 deficiency. J Clin Invest 98(11):2648-55. [PubMed: 8958230]  [MGI Ref ID J:37969]

Additional References

Beck KD; Powell-Braxton L; Widmer HR; Valverde J; Hefti F. 1995. Igf1 gene disruption results in reduced brain size, CNS hypomyelination, and loss of hippocampal granule and striatal parvalbumin-containing neurons. Neuron 14(4):717-30. [PubMed: 7718235]  [MGI Ref ID J:24953]

Powell-Braxton L; Hollingshead P; Warburton C; Dowd M; Pitts-Meek S; Dalton D; Gillett N; Stewart TA. 1993. IGF-I is required for normal embryonic growth in mice. Genes Dev 7(12B):2609-17. [PubMed: 8276243]  [MGI Ref ID J:16214]

Igf1^tm2Ts related

Palmen M; Daemen MJ; Bronsaer R; Dassen WR; Zandbergen HR; Kockx M; Smits JF; van der Zee R; Doevendans PA. 2001. Cardiac remodeling after myocardial infarction is impaired in IGF-1 deficient mice. Cardiovasc Res 50(3):516-24. [PubMed: 11376627]  [MGI Ref ID J:162752]

Ren J; Brown-Borg HM. 2002. Impaired cardiac excitation-contraction coupling in ventricular myocytes from Ames dwarf mice with IGF-I deficiency. Growth Horm IGF Res 12(2):99-105. [PubMed: 12175647]  [MGI Ref ID J:127498]

Richards RG; Klotz DM; Walker MP; Diaugustine RP. 2004. Mammary gland branching morphogenesis is diminished in mice with a deficiency of insulin-like growth factor-I (IGF-I), but not in mice with a liver-specific deletion of IGF-I. Endocrinology 145(7):3106-10. [PubMed: 15059953]  [MGI Ref ID J:105630]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.
• Genomic DNA is available for this strain from the Mouse DNA Resource.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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