Strain Name:

STOCK Tg(Trp53A135V)L3Ber/J

Stock Number:

003262

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names STOCK Tg(Trp53A135V)2Ber/J    (Changed: 15-DEC-04 )
Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationF?+2
 
Donating Investigator Alan Bernstein,   Canadian Institutes of Health Research

Important Note
This strain may be homozygous for Gnat2cpfl3, cone photoreceptor function loss 3, which affects bright light (photopic) vision.

Description
Mice homozygous for the (Trp53A135V)2Ber transgene are viable and fertile, but show a high incidence of tumors, particularly lung adenocarcinomas, osteosarcomas, and lymphomas. This strain may serve as a model for Li-Fraumeni syndrome.

Related Strains

Strains carrying   Gnat2cpfl3 allele
003072   ALS/LtJ
006180   CD10/JlsJ
005052   PN/nBSwUmabJ
002746   SENCARA/PtJ
002747   SENCARB/PtJ
002748   SENCARC/PtJ
006135   STOCK Sgk3fz-ica/McirJ
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
004623   STOCK Tg(Fos-lacZ)34Efu/J
005667   STOCK Tg(Neurog3-cre)C1Able/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
View Strains carrying   Gnat2cpfl3     (13 strains)

View Strains carrying other alleles of Trp53     (21 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Tg(Trp53A135V)L3Ber/0

        involves: C57BL/6 * CD-1
  • life span-post-weaning/aging
  • increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:73757)
    • old mice tolerate anesthetics poorly
  • premature aging (MGI Ref ID J:73757)
    • mice exhibit some phenotypes associated with premature ageing
  • premature death (MGI Ref ID J:73757)
    • decrease in median and maximal lifespans compared to wild-type controls
  • skin/coat/nails phenotype
  • alopecia (MGI Ref ID J:73757)
    • some in older mice
  • delayed hair regrowth (MGI Ref ID J:73757)
    • by 18 to 20 months of age, hair growth is observed is diminished compared to in wild-type mice
  • ruffled hair (MGI Ref ID J:73757)
    • at 18 to 20 months of age
  • sparse hair (MGI Ref ID J:73757)
    • at 18 to 20 months of age
  • tumorigenesis
  • decreased tumor incidence (MGI Ref ID J:73757)
    • mice exhibit a 20% tumor incidence by 18 months of age with most mice remaining tumor free by 18 months of age
  • lung adenocarcinoma (MGI Ref ID J:73757)
  • homeostasis/metabolism phenotype
  • delayed wound healing (MGI Ref ID J:73757)
    • at 16 to 18 months of age
  • increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:73757)
    • old mice tolerate anesthetics poorly
  • skeleton phenotype
  • kyphosis (MGI Ref ID J:73757)
    • by 18 to 20 months of age, mice exhibit lordokyphosis
  • lordosis (MGI Ref ID J:73757)
    • by 18 to 20 months of age, mice exhibit lordokyphosis
  • osteoporosis (MGI Ref ID J:73757)
    • at 18 months of age
  • adipose tissue phenotype
  • decreased subcutaneous adipose tissue amount (MGI Ref ID J:73757)
    • at 16 to 18 months of age
  • behavior/neurological phenotype
  • lethargy (MGI Ref ID J:73757)
    • at 18 to 20 months of age
  • endocrine/exocrine gland phenotype
  • decreased testis weight (MGI Ref ID J:73757)
    • at 18 months of age
  • growth/size phenotype
  • weight loss (MGI Ref ID J:73757)
    • at 18 to 20 months of age
  • hematopoietic system phenotype
  • decreased spleen weight (MGI Ref ID J:73757)
    • at 18 months of age
  • immune system phenotype
  • decreased spleen weight (MGI Ref ID J:73757)
    • at 18 months of age
  • liver/biliary system phenotype
  • decreased liver weight (MGI Ref ID J:73757)
    • at 18 months of age
  • muscle phenotype
  • decreased skeletal muscle mass (MGI Ref ID J:73757)
    • at 18 months of age
  • renal/urinary system phenotype
  • decreased kidney weight (MGI Ref ID J:73757)
    • at 18 months of age
  • reproductive system phenotype
  • decreased testis weight (MGI Ref ID J:73757)
    • at 18 months of age
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Trp53 related

Apoptosis Research
Endogenous Regulators

Cancer Research
Increased Tumor Incidence
      Lymphomas
      Other Tissues/Organs: osteosarcoma
Toxicology
Tumor Suppressor Genes

Immunology and Inflammation Research
Intracellular Signaling Molecules

Mouse/Human Gene Homologs
Li-Fraumeni syndrome

Research Tools
Toxicology Research
      B and T cell deficiency, xenograft transplant host
      drug/compound testing

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(Trp53A135V)L3Ber
Allele Name transgene insertion L3, Alan Bernstein
Allele Type Transgenic (random, expressed)
Common Name(s) Tg(Trp53A135V)2Ber; Tg(pL53)3Ber; UL53-3; p53val135; pL53-3;
Strain of OriginCD-1
Expressed Gene Trp53, transformation related protein 53, mouse, laboratory
Promoter Trp53, transformation related protein 53, mouse, laboratory
General Note Additional lines L1 and L2 were produced.

Homozygous transgenic mice are viable and fertile, but show a high incidence of tumors, particularly lung adenocarcinomas, osteosarcomas, and lymphomas.

Molecular Note The transgene contains the mouse transformation related protein 53 promoter and an allele of the mouse Trp53 gene that carries a point mutation in codon 135 (GCG to GTG), resulting in an alanine to valine substitution. The Trp53 promoter drove high levels of transgenic expression in the spleen, thymus, and lymph nodes. Transgenic expression was also reported in the ovaries. Little or no expression was reported to have been observed in the liver and lungs of these mice. [MGI Ref ID J:19870]
 
 
Allele Symbol Gnat2cpfl3
Allele Name cone photoreceptor function loss 3
Allele Type Spontaneous
Common Name(s) Gnat2;
Strain of Originvarious
Gene Symbol and Name Gnat2, guanine nucleotide binding protein, alpha transducing 2
Chromosome 3
Gene Common Name(s) ACHM4; AW490837; GNATC; Gnat-2; Gt-2; Tcalpha; expressed sequence AW490837;
General Note This allele has been detected in the following strains either by genotyping or complementation testing: ALS/LtJ, SENCARA/PtJ, SENCARB/PtJ, SENCARC/PtJ, PN/nBSwUmabJ. (J:122428)

Phenotypic Similarity to Human Syndrome in Orthologous Human Gene: OMIM +139340 ACHROMATOPSIA 4.

Molecular Note A single nucleotide substitution of G to A at position 598 in exon 6. This mutation converts codon 200 from apartic acid to asparagine. [MGI Ref ID J:122428]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Trp53A135V)L3Ber, Restriction Enzyme Digest

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Harvey M; Vogel H; Morris D; Bradley A; Bernstein A; Donehower LA. 1995. A mutant p53 transgene accelerates tumour development in heterozygous but not nullizygous p53-deficient mice. Nat Genet 9(3):305-11. [PubMed: 7773294]  [MGI Ref ID J:23262]

Additional References

Chang B; Dacey MS; Hawes NL; Hitchcock PF; Milam AH; Atmaca-Sonmez P; Nusinowitz S; Heckenlively JR. 2006. Cone photoreceptor function loss-3, a novel mouse model of achromatopsia due to a mutation in Gnat2. Invest Ophthalmol Vis Sci 47(11):5017-21. [PubMed: 17065522]  [MGI Ref ID J:122428]

Lavigueur A; Maltby V; Mock D; Rossant J; Pawson T; Bernstein A. 1989. High incidence of lung, bone, and lymphoid tumors in transgenic mice overexpressing mutant alleles of the p53 oncogene. Mol Cell Biol 9(9):3982-91. [PubMed: 2476668]  [MGI Ref ID J:19870]

Gnat2cpfl3 related

Alexander JJ; Umino Y; Everhart D; Chang B; Min SH; Li Q; Timmers AM; Hawes NL; Pang JJ; Barlow RB; Hauswirth WW. 2007. Restoration of cone vision in a mouse model of achromatopsia. Nat Med 13(6):685-7. [PubMed: 17515894]  [MGI Ref ID J:121897]

Chang B; Dacey MS; Hawes NL; Hitchcock PF; Milam AH; Atmaca-Sonmez P; Nusinowitz S; Heckenlively JR. 2006. Cone photoreceptor function loss-3, a novel mouse model of achromatopsia due to a mutation in Gnat2. Invest Ophthalmol Vis Sci 47(11):5017-21. [PubMed: 17065522]  [MGI Ref ID J:122428]

Deng WT; Sakurai K; Liu J; Dinculescu A; Li J; Pang J; Min SH; Chiodo VA; Boye SL; Chang B; Kefalov VJ; Hauswirth WW. 2009. Functional interchangeability of rod and cone transducin alpha-subunits. Proc Natl Acad Sci U S A 106(42):17681-6. [PubMed: 19815523]  [MGI Ref ID J:153749]

Nusinowitz S; Ridder WH rd; Ramirez J. 2007. Temporal response properties of the primary and secondary rod-signaling pathways in normal and Gnat2 mutant mice. Exp Eye Res 84(6):1104-14. [PubMed: 17408617]  [MGI Ref ID J:126462]

Umino Y; Solessio E; Barlow RB. 2008. Speed, spatial, and temporal tuning of rod and cone vision in mouse. J Neurosci 28(1):189-98. [PubMed: 18171936]  [MGI Ref ID J:131050]

Tg(Trp53A135V)L3Ber related

Lavigueur A; Maltby V; Mock D; Rossant J; Pawson T; Bernstein A. 1989. High incidence of lung, bone, and lymphoid tumors in transgenic mice overexpressing mutant alleles of the p53 oncogene. Mol Cell Biol 9(9):3982-91. [PubMed: 2476668]  [MGI Ref ID J:19870]

Tyner SD; Venkatachalam S; Choi J; Jones S; Ghebranious N; Igelmann H; Lu X; Soron G; Cooper B; Brayton C; Hee Park S; Thompson T; Karsenty G; Bradley A; Donehower LA. 2002. p53 mutant mice that display early ageing-associated phenotypes. Nature 415(6867):45-53. [PubMed: 11780111]  [MGI Ref ID J:73757]

Wang Y; Zhang Z; Lubet R; You M. 2005. Tobacco smoke-induced lung tumorigenesis in mutant A/J mice with alterations in K-ras, p53, or Ink4a/Arf. Oncogene 24(18):3042-9. [PubMed: 15846305]  [MGI Ref ID J:98054]

Wang Y; Zhang Z; Lubet RA; You M. 2006. A mouse model for tumor progression of lung cancer in ras and p53 transgenic mice. Oncogene 25(8):1277-80. [PubMed: 16247444]  [MGI Ref ID J:107333]

Yao R; Wang Y; D'Agostini F; Izzotti A; Lubet RA; You M; De Flora S. 2005. K-ras mutations in lung tumors from p53 mutant mice exposed to cigarette smoke. Exp Lung Res 31(2):271-81. [PubMed: 15824025]  [MGI Ref ID J:98657]

Zhang Z; Liu Q; Lantry LE; Wang Y; Kelloff GJ; Anderson MW; Wiseman RW; Lubet RA; You M. 2000. A germ-line p53 mutation accelerates pulmonary tumorigenesis: p53-independent efficacy of chemopreventive agents green tea or dexamethasone/myo-inositol and chemotherapeutic agents taxol or adriamycin. Cancer Res 60(4):901-7. [PubMed: 10706103]  [MGI Ref ID J:61217]

Zhang Z; Wang Y; Yao R; Li J; Lubet RA; You M. 2006. p53 Transgenic mice are highly susceptible to 4-nitroquinoline-1-oxide-induced oral cancer. Mol Cancer Res 4(6):401-10. [PubMed: 16778087]  [MGI Ref ID J:112187]

Zhang Z; Wang Y; Yao R; Li J; Yan Y; La Regina M; Lemon WL; Grubbs CJ; Lubet RA; You M. 2004. Cancer chemopreventive activity of a mixture of Chinese herbs (antitumor B) in mouse lung tumor models. Oncogene 23(21):3841-50. [PubMed: 15021904]  [MGI Ref ID J:89926]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.
Important Note
This strain may be homozygous for Gnat2cpfl3, cone photoreceptor function loss 3, which affects bright light (photopic) vision.

Payment Terms and Conditions

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