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Strain Name:

STOCK Tg(Trp53A135V)L3Ber/J

Stock Number:

003262

Availability:

Repository-Cryopreserved

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Former Name      STOCK Tg(Trp53A135V)2Ber/J    (Changed: 15-DEC-04 )
Genes & Alleles   Gnat2;   Gnat2cpfl3;   Trp53;   Tg(Trp53A135V)L3Ber;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Alan Bernstein,   Canadian Institutes of Health Research
GenerationF?+2

Important Note
This strain is homozygous for Gnat2cpfl3, cone photoreceptor function loss 3, which affects bright light (photopic) vision.

Strain Description
Mice homozygous for the (Trp53A135V)2Ber transgene are viable and fertile, but show a high incidence of tumors, particularly lung adenocarcinomas, osteosarcomas, and lymphomas. This strain may serve as a model for Li-Fraumeni syndrome.

Gene & Allele Details

Allele Symbol Tg(Trp53A135V)L3Ber
Allele Name transgene insertion L3, Alan Bernstein
Common Name(s) Tg(Trp53A135V)2Ber; Tg(pL53)3Ber; UL53-3; p53val135; pL53-3;
Strain of OriginCD-1
Expressed Gene Trp53, transformation related protein 53, mouse, laboratory
Promoter Trp53, transformation related protein 53, mouse, laboratory
General Note Additional lines L1 and L2 were produced.

Homozygous transgenic mice are viable and fertile, but show a high incidence of tumors, particularly lung adenocarcinomas, osteosarcomas, and lymphomas.

Molecular Note The transgene contains the mouse transformation related protein 53 promoter and an allele of the mouse Trp53 gene that carries a point mutation in codon 135 (GCG to GTG), resulting in an alanine to valine substitution. The Trp53 promoter drove high levels of transgenic expression in the spleen, thymus, and lymph nodes. Transgenic expression was also reported in the ovaries. Little or no expression was reported to have been observed in the liver and lungs of these mice. [MGI Ref ID J:19870]
 
Allele Symbol Gnat2cpfl3
Allele Name cone photoreceptor function loss 3
Common Name(s) Gnat2;
Strain of Originvarious
Gene Symbol and Name Gnat2, guanine nucleotide binding protein, alpha transducing 2
Chromosome 3
Gene Common Name(s) ACHM4; AW490837; GNATC; Gnat-2; Gt-2; Tcalpha; expressed sequence AW490837;
General Note This allele has been detected in the following strains either by genotyping or complementation testing: ALS/LtJ, SENCARA/PtJ, SENCARB/PtJ, SENCARC/PtJ, PN/nBSwUmabJ. (J:122428)

Phenotypic Similarity to Human Syndrome in Orthologous Human Gene: OMIM +139340 ACHROMATOPSIA 4.

Molecular Note A single nucleotide substitution of G to A at position 598 in exon 6. This mutation converts codon 200 from apartic acid to asparagine. [MGI Ref ID J:122428]

Genotyping Protocols

Tg(Trp53A135V)L3Ber

Related Strains

Strains carrying   Gnat2cpfl3 allele
002448   129S1/SvImJ
003072   ALS/LtJ
006180   CD10/JlsJ
005052   PN/nBSwUmabJ
002746   SENCARA/PtJ
002747   SENCARB/PtJ
002748   SENCARC/PtJ
006135   STOCK Sgk3fz-ica/McirJ
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
004623   STOCK Tg(Fos-lacZ)34Efu/J
005667   STOCK Tg(Neurog3-cre)C1Able/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
View Strains carrying   Gnat2cpfl3     (14 strains)

View Strains carrying other alleles of Trp53     (12 strains)

Research Applications

This mouse can be used to support research in many areas including:

Trp53 related

Apoptosis Research
Endogenous Regulators

Cancer Research
Increased Tumor Incidence (Lymphomas)
Increased Tumor Incidence (Other Tissues/Organs: osteosarcoma)
Toxicology
Tumor Suppressor Genes

Immunology and Inflammation Research
Intracellular Signaling Molecules

Mouse/Human Gene Homologs
Li-Fraumeni syndrome

Research Tools
Toxicology Research (B and T cell deficiency) (xenograft transplant host)
Toxicology Research (drug/compound testing)

References

Selected Reference(s)

Harvey M; Vogel H; Morris D; Bradley A; Bernstein A; Donehower LA. 1995. A mutant p53 transgene accelerates tumour development in heterozygous but not nullizygous p53-deficient mice. Nat Genet 9(3):305-11. [PubMed: 7773294]  [MGI Ref ID J:23262]

Additional References

Price and Supply Information

Strain Name: STOCK Tg(Trp53A135V)L3Ber/J
Stock Number: 003262

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

OncoMouse® requires a license from DuPont, see Licenses for Strains with OncoMouse® Technology.
P53 Mice are subject to U.S. 5,569,824 and corresponding license requirements.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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