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Genes & Alleles

Cdkn1a;   Cdkn1atm1Tyj;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Tyler Jacks,   Massachusetts Institute of Technology Generation F?+21 (08-JAN-08)

Appearance
white-bellied agouti
Related Genotype: A^w/A^w

Strain Description
Homozygotes are viable and fertile. p21, the product of the Cdkn1a gene, belongs to a family of regulators, known as cyclin-dependent kinase inhibitors, which modulate progression through the cell cycle. Following serum restimulation, expression of p21 is superinducible by cycloheximide in wild-type but not in p53-deficient cells. p53 appears to play a critical role in p21 induction following DNA damage. p21 can be regulated independently of p53 during normal tissue development, following serum stimulation, and during cellular differentiation.

Mammalian Phenotype Terms assigned by genotype Cdkn1atm1Tyj/Cdkn1atm1Tyj         B6;129S2-Cdkn1atm1Tyj/J hearing/vestibular/ear phenotype *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:98518) contrary to expectation, homozygotes display a normal inner ear morphology relative to wild type mice at E16.5, E18.5, P2 and 2 months of age, no significant differences in the cytoarchitecture of vestibular and auditory sensory epithelia or aberrant mitoses in developing hair cells are observed Cdkn1atm1Tyj/Cdkn1atm1Tyj         involves: 129S2/SvPas * C57BL/6 tumorigenesis increased incidence of chemically-induced tumors (MGI Ref ID J:80271) treatment with the chemical carcinogen, urethane, results in accelerated tumor onset and increased tumor multiplicity compared to controls; tumors are predominantly lung tumors homeostasis/metabolism phenotype increased incidence of chemically-induced tumors (MGI Ref ID J:80271) treatment with the chemical carcinogen, urethane, results in accelerated tumor onset and increased tumor multiplicity compared to controls; tumors are predominantly lung tumors The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Cdkn1atm1Tyj/Cdkn1atm1Tyj         involves: 129S2/SvPas normal phenotype no abnormal phenotype detected (MGI Ref ID J:68068) homozygotes are viable and display no apparent phenotype by seven weeks of age

Gene & Allele Details

Allele Symbol		Cdkn1atm1Tyj
Allele Name		targeted mutation 1, Tyler Jacks
Common Name(s)		Cdkn1a-; p21-; p21cip-;
Mutation Made By		Tyler Jacks,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Cdkn1a, cyclin-dependent kinase inhibitor 1A (P21)
Chromosome		17
Gene Common Name(s)		CAP20; CDKI; CDKN1; CIP1; Cdkn1; MDA-6; P21; SDI1; WAF1; cyclin dependent kinase inhibitor; mda6; p21WAF1/CIP1; p21CIP1; p21WAF;
Molecular Note		The coding sequences were replaced by a neomycin cassette. No protein was detected by immunoprecipitation of ES cell lysates in which both alleles were disrupted. [MGI Ref ID J:68068]

Control Information

Allele	Control
Cdkn1atm1Tyj	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Genotyping Protocols

Cdkn1a^tm1Tyj

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cdkn1a^tm1Tyj related

Cancer Research
Genes Regulating Growth and Proliferation
Tumor Suppressor Genes

Cardiovascular Research
Atherosclerosis

Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation

References

Selected Reference(s)

Brugarolas J; Chandrasekaran C; Gordon JI; Beach D; Jacks T; Hannon GJ. 1995. Radiation-induced cell cycle arrest compromised by p21 deficiency. Nature 377(6549):552-7. [PubMed: 7566157]  [MGI Ref ID J:68068]

Additional References

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Price and Supply Information

Strain Name:	B6;129S2-Cdkn1atm1Tyj/J
Stock Number:	003263

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

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- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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