Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation F?+21 (08-JAN-08)   Donating Investigator Tyler Jacks,   Massachusetts Institute of Technology

Appearance
white-bellied agouti
Related Genotype: A^w/A^w

Description
Homozygotes are viable and fertile. p21, the product of the Cdkn1a gene, belongs to a family of regulators, known as cyclin-dependent kinase inhibitors, which modulate progression through the cell cycle. Following serum restimulation, expression of p21 is superinducible by cycloheximide in wild-type but not in p53-deficient cells. p53 appears to play a critical role in p21 induction following DNA damage. p21 can be regulated independently of p53 during normal tissue development, following serum stimulation, and during cellular differentiation.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying   Cdkn1a^tm1Tyj allele

008184

129S/Sv-Cdkn1atm1Tyj/J

View Strains carrying   Cdkn1a^tm1Tyj     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cdkn1a^tm1Tyj/Cdkn1a^tm1Tyj

        B6;129S2-Cdkn1a^tm1Tyj/J

• hearing/vestibular/ear phenotype
• *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:98518)
  • contrary to expectation, homozygotes display a normal inner ear morphology relative to wild-type mice
  • at E16.5, E18.5, P2 and 2 months of age, no significant differences in the cytoarchitecture of vestibular and auditory sensory epithelia or aberrant mitoses in developing hair cells are observed

Cdkn1a^tm1Tyj/Cdkn1a^tm1Tyj

        involves: 129S2/SvPas * C57BL/6

• tumorigenesis
• increased incidence of chemically-induced tumors (MGI Ref ID J:80271)
  • treatment with the chemical carcinogen, urethane, results in accelerated tumor onset and increased tumor multiplicity compared to controls; tumors are predominantly lung tumors
• homeostasis/metabolism phenotype
• increased incidence of chemically-induced tumors (MGI Ref ID J:80271)
  • treatment with the chemical carcinogen, urethane, results in accelerated tumor onset and increased tumor multiplicity compared to controls; tumors are predominantly lung tumors

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cdkn1a^tm1Tyj/Cdkn1a^tm1Tyj

        involves: 129S2/SvPas

• normal phenotype
• no abnormal phenotype detected (MGI Ref ID J:68068)
  • homozygotes are viable and display no apparent phenotype by seven weeks of age

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cdkn1a^tm1Tyj related

Cancer Research
Genes Regulating Growth and Proliferation
Tumor Suppressor Genes

Cardiovascular Research
Atherosclerosis

Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cdkn1atm1Tyj
Allele Name		targeted mutation 1, Tyler Jacks
Allele Type		Targeted (knock-out)
Common Name(s)		Cdkn1a-; p21-; p21cip-;
Mutation Made By		Tyler Jacks,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Cdkn1a, cyclin-dependent kinase inhibitor 1A (P21)
Chromosome		17
Gene Common Name(s)		CAP20; CDKI; CDKN1; CIP1; Cdkn1; MDA-6; P21; SDI1; WAF1; cyclin dependent kinase inhibitor; mda6; p21WAF1/CIP1; p21CIP1; p21WAF;
Molecular Note		The coding sequences were replaced by a neomycin cassette. No protein was detected by immunoprecipitation of ES cell lysates in which both alleles were disrupted. [MGI Ref ID J:68068]

Genotyping

Genotyping Information

Genotyping Protocols

Cdkn1a^tm1Tyj, SEP PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Brugarolas J; Chandrasekaran C; Gordon JI; Beach D; Jacks T; Hannon GJ. 1995. Radiation-induced cell cycle arrest compromised by p21 deficiency. Nature 377(6549):552-7. [PubMed: 7566157]  [MGI Ref ID J:68068]

Additional References

Carstens MJ; Krempler A; Triplett AA; Van Lohuizen M; Wagner KU. 2004. Cell cycle arrest and cell death are controlled by p53-dependent and p53-independent mechanisms in Tsg101-deficient cells. J Biol Chem 279(34):35984-94. [PubMed: 15210712]  [MGI Ref ID J:92528]

Hawke TJ; Jiang N; Garry DJ. 2003. Absence of p21CIP Rescues Myogenic Progenitor Cell Proliferative and Regenerative Capacity in Foxk1 Null Mice. J Biol Chem 278(6):4015-20. [PubMed: 12446708]  [MGI Ref ID J:81688]

Hawke TJ; Meeson AP; Jiang N; Graham S; Hutcheson K; DiMaio JM; Garry DJ. 2003. p21 is essential for normal myogenic progenitor cell function in regenerating skeletal muscle. Am J Physiol Cell Physiol 285(5):C1019-27. [PubMed: 12826599]  [MGI Ref ID J:86537]

Macleod KF; Sherry N; Hannon G; Beach D; Tokino T; Kinzler K; Vogelstein B; Jacks T. 1995. p53-dependent and independent expression of p21 during cell growth, differentiation, and DNA damage. Genes Dev 9(8):935-44. [PubMed: 7774811]  [MGI Ref ID J:25096]

Shimura T; Inoue M; Taga M; Shiraishi K; Uematsu N; Takei N; Yuan ZM; Shinohara T; Niwa O. 2002. p53-dependent S-phase damage checkpoint and pronuclear cross talk in mouse zygotes with X-irradiated sperm. Mol Cell Biol 22(7):2220-8. [PubMed: 11884608]  [MGI Ref ID J:88409]

Song R; Mahidhara RS; Zhou Z; Hoffman RA; Seol DW; Flavell RA; Billiar TR; Otterbein LE; Choi AM. 2004. Carbon monoxide inhibits T lymphocyte proliferation via caspase-dependent pathway. J Immunol 172(2):1220-6. [PubMed: 14707100]  [MGI Ref ID J:87634]

Verdoodt B; Blazek T; Rauch P; Schuler G; Steinkasserer A; Lutz MB; Funk JO. 2003. The cyclin-dependent kinase inhibitors p27Kip1 and p21Cip1 are not essential in T cell anergy. Eur J Immunol 33(11):3154-63. [PubMed: 14579284]  [MGI Ref ID J:86442]

Cdkn1a^tm1Tyj related

Adnane J; Jackson RJ; Nicosia SV; Cantor AB; Pledger WJ; Sebti SM. 2000. Loss of p21WAF1/CIP1 accelerates Ras oncogenesis in a transgenic/knockout mammary cancer model Oncogene 19(47):5338-47. [PubMed: 11103935]  [MGI Ref ID J:66183]

Aikawa T; Segre GV; Lee K. 2001. Fibroblast Growth Factor Inhibits Chondrocytic Growth through Induction of p21 and Subsequent Inactivation of Cyclin E-Cdk2. J Biol Chem 276(31):29347-52. [PubMed: 11384971]  [MGI Ref ID J:70717]

Al-Douahji M; Brugarolas J; Brown PA; Stehman-Breen CO; Alpers CE; Shankland SJ. 1999. The cyclin kinase inhibitor p21WAF1/CIP1 is required for glomerular hypertrophy in experimental diabetic nephropathy. Kidney Int 56(5):1691-9. [PubMed: 10571777]  [MGI Ref ID J:59647]

Baena E; Ortiz M; Martinez-A C; de Alboran IM. 2007. c-Myc is essential for hematopoietic stem cell differentiation and regulates Lin(-)Sca-1(+)c-Kit(-) cell generation through p21. Exp Hematol 35(9):1333-43. [PubMed: 17637497]  [MGI Ref ID J:126563]

Balomenos D; Martin-Caballero J; Garcia MI; Prieto I; Flores JM; Serrano M; Martinez-A C. 2000. The cell cycle inhibitor p21 controls T-cell proliferation and sex-linked lupus development. Nat Med 6(2):171-6. [PubMed: 10655105]  [MGI Ref ID J:60292]

Barboza JA; Liu G; Ju Z; El-Naggar AK; Lozano G. 2006. p21 delays tumor onset by preservation of chromosomal stability. Proc Natl Acad Sci U S A 103(52):19842-7. [PubMed: 17170138]  [MGI Ref ID J:118255]

Bishop AJ; Kosaras B; Hollander MC; Fornace A Jr; Sidman RL; Schiestl RH. 2006. p21 controls patterning but not homologous recombination in RPE development. DNA Repair (Amst) 5(1):111-20. [PubMed: 16202662]  [MGI Ref ID J:104280]

Carstens MJ; Krempler A; Triplett AA; Van Lohuizen M; Wagner KU. 2004. Cell cycle arrest and cell death are controlled by p53-dependent and p53-independent mechanisms in Tsg101-deficient cells. J Biol Chem 279(34):35984-94. [PubMed: 15210712]  [MGI Ref ID J:92528]

Chavez-Reyes A; Parant JM; Amelse LL; de Oca Luna RM; Korsmeyer SJ; Lozano G. 2003. Switching mechanisms of cell death in mdm2- and mdm4-null mice by deletion of p53 downstream targets. Cancer Res 63(24):8664-9. [PubMed: 14695178]  [MGI Ref ID J:87065]

Cheng T; Rodrigues N; Shen H; Yang Y; Dombkowski D; Sykes M; Scadden DT. 2000. Hematopoietic stem cell quiescence maintained by p21cip1/waf1. Science 287(5459):1804-8. [PubMed: 10710306]  [MGI Ref ID J:60945]

Chesnokova V; Zonis S; Rubinek T; Yu R; Ben-Shlomo A; Kovacs K; Wawrowsky K; Melmed S. 2007. Senescence mediates pituitary hypoplasia and restrains pituitary tumor growth. Cancer Res 67(21):10564-72. [PubMed: 17975001]  [MGI Ref ID J:127141]

Cozar-Castellano I; Haught M; Stewart AF. 2006. The cell cycle inhibitory protein p21cip is not essential for maintaining beta-cell cycle arrest or beta-cell function in vivo. Diabetes 55(12):3271-8. [PubMed: 17130470]  [MGI Ref ID J:121017]

Duckers HJ; Boehm M; True AL; Yet SF; San H; Park JL; Clinton Webb R; Lee ME; Nabel GJ; Nabel EG. 2001. Heme oxygenase-1 protects against vascular constriction and proliferation. Nat Med 7(6):693-8. [PubMed: 11385506]  [MGI Ref ID J:106600]

Engel FB; Hauck L; Boehm M; Nabel EG; Dietz R; von Harsdorf R. 2003. p21(CIP1) Controls proliferating cell nuclear antigen level in adult cardiomyocytes. Mol Cell Biol 23(2):555-65. [PubMed: 12509454]  [MGI Ref ID J:127027]

Engelman RW; Jackson RJ; Coppola D; Wharton W; Cantor AB; Pledger WJ. 2007. Loss of nuclear p21(Cip1/WAF1) during neoplastic progression to metastasis in gamma-irradiated p21 hemizygous mice. Exp Mol Pathol 82(3):234-44. [PubMed: 17207793]  [MGI Ref ID J:121797]

Hakem R; de la Pompa JL; Elia A; Potter J; Mak TW. 1997. Partial rescue of Brca1 (5-6) early embryonic lethality by p53 or p21 null mutation. Nat Genet 16(3):298-302. [PubMed: 9207798]  [MGI Ref ID J:41254]

Harms C; Albrecht K; Harms U; Seidel K; Hauck L; Baldinger T; Hubner D; Kronenberg G; An J; Ruscher K; Meisel A; Dirnagl U; von Harsdorf R; Endres M; Hortnagl H. 2007. Phosphatidylinositol 3-Akt-kinase-dependent phosphorylation of p21(Waf1/Cip1) as a novel mechanism of neuroprotection by glucocorticoids. J Neurosci 27(17):4562-71. [PubMed: 17460069]  [MGI Ref ID J:121086]

Hawke TJ; Jiang N; Garry DJ. 2003. Absence of p21CIP Rescues Myogenic Progenitor Cell Proliferative and Regenerative Capacity in Foxk1 Null Mice. J Biol Chem 278(6):4015-20. [PubMed: 12446708]  [MGI Ref ID J:81688]

Hawke TJ; Meeson AP; Jiang N; Graham S; Hutcheson K; DiMaio JM; Garry DJ. 2003. p21 is essential for normal myogenic progenitor cell function in regenerating skeletal muscle. Am J Physiol Cell Physiol 285(5):C1019-27. [PubMed: 12826599]  [MGI Ref ID J:86537]

Inoue N; Yahagi N; Yamamoto T; Ishikawa M; Watanabe K; Matsuzaka T; Nakagawa Y; Takeuchi Y; Kobayashi K; Takahashi A; Suzuki H; Hasty AH; Toyoshima H; Yamada N; Shimano H. 2008. Cyclin-dependent kinase inhibitor, p21WAF1/CIP1, is involved in adipocyte differentiation and hypertrophy, linking to obesity, and insulin resistance. J Biol Chem 283(30):21220-9. [PubMed: 18445590]  [MGI Ref ID J:137950]

Jackson RJ; Adnane J; Coppola D; Cantor A; Sebti SM; Pledger WJ. 2002. Loss of the cell cycle inhibitors p21(Cip1) and p27(Kip1) enhances tumorigenesis in knockout mouse models. Oncogene 21(55):8486-97. [PubMed: 12466968]  [MGI Ref ID J:80271]

Jackson RJ; Engelman RW; Coppola D; Cantor AB; Wharton W; Pledger WJ. 2003. p21Cip1 Nullizygosity increases tumor metastasis in irradiated mice. Cancer Res 63(12):3021-5. [PubMed: 12810620]  [MGI Ref ID J:83967]

Jaime M; Pujol MJ; Serratosa J; Pantoja C; Canela N; Casanovas O; Serrano M; Agell N; Bachs O. 2002. The p21(Cip1) protein, a cyclin inhibitor, regulates the levels and the intracellular localization of CDC25A in mice regenerating livers. Hepatology 35(5):1063-71. [PubMed: 11981756]  [MGI Ref ID J:105978]

Jiang Z; Liang P; Leng R; Guo Z; Liu Y; Liu X; Bubnic S; Keating A; Murray D; Goss P; Zacksenhaus E. 2000. E2F1 and p53 are dispensable, whereas p21(Waf1/Cip1) cooperates with Rb to restrict endoreduplication and apoptosis during skeletal myogenesis Dev Biol 227(1):28-41. [PubMed: 11076674]  [MGI Ref ID J:65679]

Jirawatnotai S; Moons DS; Stocco CO; Franks R; Hales DB; Gibori G; Kiyokawa H. 2003. The cyclin-dependent kinase inhibitors p27Kip1 and p21Cip1 cooperate to restrict proliferative life span in differentiating ovarian cells. J Biol Chem 278(19):17021-7. [PubMed: 12609976]  [MGI Ref ID J:83300]

Kippin TE; Martens DJ; van der Kooy D. 2005. p21 loss compromises the relative quiescence of forebrain stem cell proliferation leading to exhaustion of their proliferation capacity. Genes Dev 19(6):756-67. [PubMed: 15769947]  [MGI Ref ID J:105510]

Komarova EA; Christov K; Faerman AI; Gudkov AV. 2000. Different impact of p53 and p21 on the radiation response of mouse tissues. Oncogene 19(33):3791-8. [PubMed: 10949934]  [MGI Ref ID J:63921]

Komarova EA; Kondratov RV; Wang K; Christov K; Golovkina TV; Goldblum JR; Gudkov AV. 2004. Dual effect of p53 on radiation sensitivity in vivo: p53 promotes hematopoietic injury, but protects from gastro-intestinal syndrome in mice. Oncogene 23(19):3265-71. [PubMed: 15064735]  [MGI Ref ID J:89739]

Kunieda T; Minamino T; Nishi J; Tateno K; Oyama T; Katsuno T; Miyauchi H; Orimo M; Okada S; Takamura M; Nagai T; Kaneko S; Komuro I. 2006. Angiotensin II induces premature senescence of vascular smooth muscle cells and accelerates the development of atherosclerosis via a p21-dependent pathway. Circulation 114(9):953-60. [PubMed: 16908765]  [MGI Ref ID J:125247]

Kwon YH; Jovanovic A; Serfas MS; Tyner AL. 2003. The Cdk inhibitor p21 is required for necrosis, but it inhibits apoptosis following toxin-induced liver injury. J Biol Chem 278(32):30348-55. [PubMed: 12759355]  [MGI Ref ID J:84930]

Laine H; Doetzlhofer A; Mantela J; Ylikoski J; Laiho M; Roussel MF; Segil N; Pirvola U. 2007. p19(Ink4d) and p21(Cip1) collaborate to maintain the postmitotic state of auditory hair cells, their codeletion leading to DNA damage and p53-mediated apoptosis. J Neurosci 27(6):1434-44. [PubMed: 17287518]  [MGI Ref ID J:118337]

Li N; Zhang Y; Naylor MJ; Schatzmann F; Maurer F; Wintermantel T; Schuetz G; Mueller U; Streuli CH; Hynes NE. 2005. Beta1 integrins regulate mammary gland proliferation and maintain the integrity of mammary alveoli. EMBO J 24(11):1942-53. [PubMed: 15889143]  [MGI Ref ID J:99405]

Li X; Plett PA; Yang Y; Hong P; Freie B; Srour EF; Orschell CM; Clapp DW; Haneline LS. 2003. Fanconi anemia type C-deficient hematopoietic stem/progenitor cells exhibit aberrant cell cycle control. Blood 102(6):2081-4. [PubMed: 12763929]  [MGI Ref ID J:115721]

Lu X; Yang C; Hill R; Yin C; Hollander MC; Fornace AJ Jr; Van Dyke T. 2008. Inactivation of gadd45a sensitizes epithelial cancer cells to ionizing radiation in vivo resulting in prolonged survival. Cancer Res 68(10):3579-83. [PubMed: 18483238]  [MGI Ref ID J:135074]

Maeda T; Espino RA; Chomey EG; Luong L; Bano A; Meakins D; Tron VA. 2005. Loss of p21WAF1/Cip1 in Gadd45-deficient keratinocytes restores DNA repair capacity. Carcinogenesis 26(10):1804-10. [PubMed: 15917306]  [MGI Ref ID J:101710]

Mantela J; Jiang Z; Ylikoski J; Fritzsch B; Zacksenhaus E; Pirvola U. 2005. The retinoblastoma gene pathway regulates the postmitotic state of hair cells of the mouse inner ear. Development 132(10):2377-88. [PubMed: 15843406]  [MGI Ref ID J:98518]

Martin A; Odajima J; Hunt SL; Dubus P; Ortega S; Malumbres M; Barbacid M. 2005. Cdk2 is dispensable for cell cycle inhibition and tumor suppression mediated by p27(Kip1) and p21(Cip1). Cancer Cell 7(6):591-8. [PubMed: 15950907]  [MGI Ref ID J:98948]

Martin-Caballero J; Flores JM; Garcia-Palencia P; Collado M; Serrano M. 2004. Different cooperating effect of p21 or p27 deficiency in combination with INK4a/ARF deletion in mice. Oncogene 23(50):8231-7. [PubMed: 15378017]  [MGI Ref ID J:93856]

Martin-Caballero J; Flores JM; Garcia-Palencia P; Serrano M. 2001. Tumor susceptibility of p21(Waf1/Cip1)-deficient mice. Cancer Res 61(16):6234-8. [PubMed: 11507077]  [MGI Ref ID J:70894]

Matheu A; Klatt P; Serrano M. 2005. Regulation of the INK4a/ARF locus by histone deacetylase inhibitors. J Biol Chem 280(51):42433-41. [PubMed: 16251190]  [MGI Ref ID J:106797]

McGrath-Morrow SA; Cho C; Soutiere S; Mitzner W; Tuder R. 2004. The effect of neonatal hyperoxia on the lung of p21Waf1/Cip1/Sdi1-deficient mice. Am J Respir Cell Mol Biol 30(5):635-40. [PubMed: 14607813]  [MGI Ref ID J:98064]

Monkawa T; Pippin J; Yo Y; Kopp JB; Alpers CE; Shankland SJ. 2006. The cyclin-dependent kinase inhibitor p21 limits murine mesangial proliferative glomerulonephritis. Nephron Exp Nephrol 102(1):e8-18. [PubMed: 16179805]  [MGI Ref ID J:127479]

Montes de Oca Luna R; Amelse LL; Chavez-Reyes A; Evans SC; Brugarolas J ; Jacks T ; Lozano G. 1997. Deletion of p21 cannot substitute for p53 loss in rescue of mdm2 null lethality [letter] Nat Genet 16(4):336-7. [PubMed: 9241268]  [MGI Ref ID J:42048]

Mukai M; Dong Q; Hardy MP; Kiyokawa H; Peterson RE; Cooke PS. 2005. Altered prostatic epithelial proliferation and apoptosis, prostatic development, and serum testosterone in mice lacking cyclin-dependent kinase inhibitors. Biol Reprod 73(5):951-8. [PubMed: 16014817]  [MGI Ref ID J:115443]

Naaz A; Holsberger DR; Iwamoto GA; Nelson A; Kiyokawa H; Cooke PS. 2004. Loss of cyclin-dependent kinase inhibitors produces adipocyte hyperplasia and obesity. FASEB J 18(15):1925-7. [PubMed: 15469964]  [MGI Ref ID J:118516]

Olive M; Mellad JA; Beltran LE; Ma M; Cimato T; Noguchi AC; San H; Childs R; Kovacic JC; Boehm M. 2008. p21Cip1 modulates arterial wound repair through the stromal cell-derived factor-1/CXCR4 axis in mice. J Clin Invest 118(6):2050-61. [PubMed: 18464929]  [MGI Ref ID J:137732]

Oskarsson T; Essers MA; Dubois N; Offner S; Dubey C; Roger C; Metzger D; Chambon P; Hummler E; Beard P; Trumpp A. 2006. Skin epidermis lacking the c-myc gene is resistant to Ras-driven tumorigenesis but can reacquire sensitivity upon additional loss of the p21Cip1 gene. Genes Dev 20(15):2024-9. [PubMed: 16882980]  [MGI Ref ID J:111139]

Panic L; Tamarut S; Sticker-Jantscheff M; Barkic M; Solter D; Uzelac M; Grabusic K; Volarevic S. 2006. Ribosomal protein S6 gene haploinsufficiency is associated with activation of a p53-dependent checkpoint during gastrulation. Mol Cell Biol 26(23):8880-91. [PubMed: 17000767]  [MGI Ref ID J:117590]

Paramio JM; Segrelles C; Ruiz S; Martin-Caballero J; Page A; Martinez J; Serrano M; Jorcano JL. 2001. The ink4a/arf Tumor Suppressors Cooperate with p21cip1/waf in the Processes of Mouse Epidermal Differentiation, Senescence, and Carcinogenesis. J Biol Chem 276(47):44203-11. [PubMed: 11551927]  [MGI Ref ID J:72780]

Pechnick RN; Zonis S; Wawrowsky K; Pourmorady J; Chesnokova V. 2008. p21Cip1 restricts neuronal proliferation in the subgranular zone of the dentate gyrus of the hippocampus. Proc Natl Acad Sci U S A 105(4):1358-63. [PubMed: 18172194]  [MGI Ref ID J:131040]

Perez-Mancera PA; Perez-Caro M; Gonzalez-Herrero I; Flores T; Orfao A; de Herreros AG; Gutierrez-Adan A; Pintado B; Sagrera A; Sanchez-Martin M; Sanchez-Garcia I. 2005. Cancer development induced by graded expression of Snail in mice. Hum Mol Genet 14(22):3449-61. [PubMed: 16207734]  [MGI Ref ID J:104124]

Poole AJ; Heap D; Carroll RE; Tyner AL. 2004. Tumor suppressor functions for the Cdk inhibitor p21 in the mouse colon. Oncogene 23(49):8128-34. [PubMed: 15377995]  [MGI Ref ID J:93857]

Qiu J; Takagi Y; Harada J; Rodrigues N; Moskowitz MA; Scadden DT; Cheng T. 2004. Regenerative response in ischemic brain restricted by p21cip1/waf1. J Exp Med 199(7):937-45. [PubMed: 15067031]  [MGI Ref ID J:90479]

Quereda V; Martinalbo J; Dubus P; Carnero A; Malumbres M. 2007. Genetic cooperation between p21Cip1 and INK4 inhibitors in cellular senescence and tumor suppression. Oncogene 26(55):7665-74. [PubMed: 17599058]  [MGI Ref ID J:129474]

Scatizzi JC; Hutcheson J; Bickel E; Woods JM; Klosowska K; Moore TL; Haines GK rd; Perlman H. 2006. p21Cip1 is required for the development of monocytes and their response to serum transfer-induced arthritis. Am J Pathol 168(5):1531-41. [PubMed: 16651620]  [MGI Ref ID J:108569]

Sharma N; Timmers C; Trikha P; Saavedra HI; Obery A; Leone G. 2006. Control of the p53-p21CIP1 Axis by E2f1, E2f2, and E2f3 is essential for G1/S progression and cellular transformation. J Biol Chem 281(47):36124-31. [PubMed: 17008321]  [MGI Ref ID J:117634]

Shimura T; Inoue M; Taga M; Shiraishi K; Uematsu N; Takei N; Yuan ZM; Shinohara T; Niwa O. 2002. p53-dependent S-phase damage checkpoint and pronuclear cross talk in mouse zygotes with X-irradiated sperm. Mol Cell Biol 22(7):2220-8. [PubMed: 11884608]  [MGI Ref ID J:88409]

Song S; Lambert PF. 1999. Different responses of epidermal and hair follicular cells to radiation correlate with distinct patterns of p53 and p21 induction. Am J Pathol 155(4):1121-7. [PubMed: 10514395]  [MGI Ref ID J:114161]

Stehr W; Bernal NP; Erwin CR; Bernabe KQ; Guo J; Warner BW. 2006. Roles for p21waf1/cip1 and p27kip1 during the adaptation response to massive intestinal resection. Am J Physiol Gastrointest Liver Physiol 290(5):G933-41. [PubMed: 16322092]  [MGI Ref ID J:111081]

Stoyanova T; Yoon T; Kopanja D; Mokyr MB; Raychaudhuri P. 2008. The xeroderma pigmentosum group E gene product DDB2 activates nucleotide excision repair by regulating the level of p21Waf1/Cip1. Mol Cell Biol 28(1):177-87. [PubMed: 17967871]  [MGI Ref ID J:128935]

TeKippe M; Harrison DE; Chen J. 2003. Expansion of hematopoietic stem cell phenotype and activity in Trp53-null mice. Exp Hematol 31(6):521-7. [PubMed: 12829028]  [MGI Ref ID J:115677]

Verdoodt B; Blazek T; Rauch P; Schuler G; Steinkasserer A; Lutz MB; Funk JO. 2003. The cyclin-dependent kinase inhibitors p27Kip1 and p21Cip1 are not essential in T cell anergy. Eur J Immunol 33(11):3154-63. [PubMed: 14579284]  [MGI Ref ID J:86442]

Wada T; Pippin JW; Terada Y; Shankland SJ. 2005. The cyclin-dependent kinase inhibitor p21 is required for TGF-beta1-induced podocyte apoptosis. Kidney Int 68(4):1618-29. [PubMed: 16164639]  [MGI Ref ID J:128639]

Watanabe K; Petro BJ; Sevandal M; Anshuman S; Jovanovic A; Tyner AL. 2004. Histochemical examination of periodontal junctional epithelium in p21/p27 double knockout mice. Eur J Oral Sci 112(3):253-8. [PubMed: 15154924]  [MGI Ref ID J:102001]

Willenbring H; Sharma AD; Vogel A; Lee AY; Rothfuss A; Wang Z; Finegold M; Grompe M. 2008. Loss of p21 permits carcinogenesis from chronically damaged liver and kidney epithelial cells despite unchecked apoptosis. Cancer Cell 14(1):59-67. [PubMed: 18598944]  [MGI Ref ID J:138701]

Woo M; Hakem R; Furlonger C; Hakem A; Duncan GS; Sasaki T; Bouchard D; Lu L; Wu GE; Paige CJ; Mak TW. 2003. Caspase-3 regulates cell cycle in B cells: a consequence of substrate specificity. Nat Immunol 4(10):1016-22. [PubMed: 12970760]  [MGI Ref ID J:85809]

Xu Y; Yang EM; Brugarolas J; Jacks T; Baltimore D. 1998. Involvement of p53 and p21 in cellular defects and tumorigenesis in Atm-/- mice. Mol Cell Biol 18(7):4385-90. [PubMed: 9632822]  [MGI Ref ID J:49262]

Yu H; Yuan Y; Shen H; Cheng T. 2006. Hematopoietic stem cell exhaustion impacted by p18 INK4C and p21 Cip1/Waf1 in opposite manners. Blood 107(3):1200-6. [PubMed: 16234365]  [MGI Ref ID J:127585]

Zhang L; Anglesio MS; O'sullivan M; Zhang F; Yang G; Sarao R; Nghiem MP; Cronin S; Hara H; Melnyk N; Li L; Wada T; Liu PP; Farrar J; Arceci RJ; Sorensen PH; Penninger JM. 2007. The E3 ligase HACE1 is a critical chromosome 6q21 tumor suppressor involved in multiple cancers. Nat Med 13(9):1060-1069. [PubMed: 17694067]  [MGI Ref ID J:125183]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.