Strain Name:

STOCK Tg(CMV-tTA)3Bjd/J

Stock Number:

003271

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names STOCK Tg(tTAhCMV)3Bjd/J    (Changed: 01-AUG-11 )
STOCK Tg(tTAhCMV)3Uh/J    (Changed: 15-DEC-04 )
Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating InvestigatorDr. Hermann Bujard,   Universitšt Heidelberg

Appearance
albino
Related Genotype: Tyrc/Tyrc

black
Related Genotype: a/a

Description
These mice were imported to The Jackson Laboratory in 1998. In 2005-2006, we confirmed that these mice express little or no tetracycline-controlled transactivator protein (tTA), even in the absence of doxycycline (dox). A similar conclusion was reported in a recent publication (Frugier et al. 2005. Genesis 42:1-5). The phenotype described below is base on the description of the mice in the original publication (Kistner et al, Proc Natl Acad Sci USA 1996 93:10933-8):

STOCK Tg(tTAhCMV)3Bjd/J mice are viable, fertile, and display no overt phenotype. These mice express a tetracycline-controlled transactivator protein (tTA) driven by the human early cytomegalovirus promoter (PhCMV. When transgenic mice are mated to a strain carrying a luciferase reporter gene coupled to a tetracycline-responsive promoter element (TRE; tetO), luciferase was expressed in multiple organs and tissues where PhCMV was known to be active (e.g. muscle, kidney, thymus, heart, pancreas) in the bitransgenic offspring. Treatment with dox reduced the luciferase activity to background levels in most organs; in some cases, the level of luciferase activity was reduced 105-fold in the presence of dox. STOCK Tg(tTAhCMV)3Bjd/J mice may be mated to strains containing a gene of interest coupled to a TRE to study the effects of expression of the target gene under tissue-specific, dox-inducible regulation. Dox may be administered in the animals' water supply.

Development
These mice were designed to express a tetracycline-controlled transactivator protein (tTA) driven by the human early cytomegalovirus promoter (PhCMV). The strain was generated on an NMRI background and was imported on the NMRI (outbred) background. The strain was mated once to C57BL/6J at The Jackson Laboratory.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of tTA
008079   129S-Ppargtm2Yba/J
016198   129S6.Cg-Tg(Camk2a-tTA)1Mmay/JlwsJ
011008   B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J
009602   B6.129S4(Cg)-Kcnn2tm2Jpad/J
009603   B6.129S4-Kcnn3tm1Jpad/J
008227   B6.129S4-Ppargtm3Yba/J
012359   B6.Cg-Pvalbtm1.1(tTA2)Hze/J
016868   B6.Cg-Ssttm1.2(tTA2)Hze/J
007004   B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ
003563   B6.Cg-Tg(Cebpb-tTA)5Bjd/J
003767   B6.Cg-Tg(Eno2tTA)5021Nes/J
003763   B6.Cg-Tg(Eno2tTA)5030Nes/J
018306   B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J
005964   B6.Cg-Tg(GFAP-tTA)110Pop/J
002618   B6.Cg-Tg(MMTVtTA)1Mam/J
008284   B6.Cg-Tg(Scg2-tTA)1Jt/J
023970   B6.Cg-Tg(Sirpa-tTA)AUmri/J
023971   B6.Cg-Tg(Sirpa-tTA)SUmri/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
017722   B6.Cg-Tg(Tal1-tTA)19Dgt/J
017754   B6;129-Omptm1(tTA)Gogo/J
007585   B6;129S4-Npytm2Rpa/J
002709   B6;C3-Tg(TettTALuc)1Dgs/J
003010   B6;CBA-Tg(Camk2a-tTA)1Mmay/J
008344   B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
010573   B6;SJL-Tg(Prl-tTA)6-5Jek/J
008082   B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J
008603   C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J
010712   C57BL/6-Tg(Camk2a-tTA)1Stl/J
013585   FVB-Tg(Cdh5-tTA)D5Lbjn/J
005625   FVB-Tg(Pcp2-tTA)3Horr/J
003170   FVB.Cg-Tg(Myh6-tTA)6Smbf/J
006209   FVB.Cg-Tg(Tal1-tTA)19Dgt/J
005942   FVB/N-Tg(Pf4-tTA/VP16)42Kra/J
004937   NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ
006999   STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J
008335   STOCK Foxa2tm1.1(rtTa)Moon/J
008600   STOCK Gt(ROSA)26Sortm1(tTA)Roos/J
005701   STOCK Pdx1tm1Macd/J
014092   STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J
024854   STOCK Tg(Camk2a-tTA)1Mmay Tg(tetO-MAPT*P301L)#Kha/J
018124   STOCK Tg(Prnp-tTA)F959Sbp/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
003275   STOCK Tg(tetL)1Bjd/J
003274   STOCK Tg(tetNZL)2Bjd/J
016970   STOCK Tg(tetO-HCV)1Mlch/Mmjax
View Strains carrying other alleles of tTA     (46 strains)

View Strains carrying other alleles of hCMV     (3 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Genetics Research
      Tissue/Cell Markers
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(CMV-tTA)3Bjd
Allele Name transgene insertion 3, Hermann Bujard
Allele Type Transgenic (random, expressed)
Common Name(s) CMV-tTA; Tg(tTAhCMV)3Bjd; Tg(tTAhCMV)3Uh;
Mutation Made ByDr. Hermann Bujard,   Universitšt Heidelberg
Strain of OriginNMRI
Site of ExpressionExpresses tTA in those tissue where the hCMV minimal promoter is active. tTA expression is detected in muscle, kidney, stomach, thymus, heart and pancreas. (NB: Studies conducted in house in 1996, suggest that these mice express little or no tTA, even in the absence of doxycycline.)
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter hCMV, human cytomegalovirus, human
General Note Transgenic mice are viable, fertile, and display no overt phenotype.
Molecular Note The transgenic construct contained sequence encoding a doxycycline-responsive tetracycline transactivator (tTA) driven by the human early cytomegalovirus promoter. [MGI Ref ID J:93000]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(tTA), Melt Curve Analysis
Tg(tTA), QPCR
Tg(tTA), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kistner A; Gossen M; Zimmermann F; Jerecic J; Ullmer C; Lubbert H; Bujard H. 1996. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci U S A 93(20):10933-8. [PubMed: 8855286]  [MGI Ref ID J:93000]

Additional References

Tg(CMV-tTA)3Bjd related

Eckenstein FP; McGovern T; Kern D; Deignan J. 2006. Neuronal vulnerability in transgenic mice expressing an inducible dominant-negative FGF receptor. Exp Neurol 198(2):338-49. [PubMed: 16487970]  [MGI Ref ID J:107899]

Griffin KJ; Kirschner LS; Matyakhina L; Stergiopoulos S; Robinson-White A; Lenherr S; Weinberg FD; Claflin E; Meoli E; Cho-Chung YS; Stratakis CA. 2004. Down-regulation of regulatory subunit type 1A of protein kinase A leads to endocrine and other tumors. Cancer Res 64(24):8811-5. [PubMed: 15604237]  [MGI Ref ID J:94953]

Griffin KJ; Kirschner LS; Matyakhina L; Stergiopoulos SG; Robinson-White A; Lenherr SM; Weinberg FD; Claflin ES; Batista D; Bourdeau I; Voutetakis A; Sandrini F; Meoli EM; Bauer AJ; Cho-Chung YS; Bornstein SR; Carney JA; Stratakis CA. 2004. A transgenic mouse bearing an antisense construct of regulatory subunit type 1A of protein kinase A develops endocrine and other tumours: comparison with Carney complex and other PRKAR1A induced lesions. J Med Genet 41(12):923-31. [PubMed: 15591278]  [MGI Ref ID J:95465]

Stanger BZ; Tanaka AJ; Melton DA. 2007. Organ size is limited by the number of embryonic progenitor cells in the pancreas but not the liver. Nature 445(7130):886-91. [PubMed: 17259975]  [MGI Ref ID J:118596]

Tilli MT; Furth PA. 2003. Conditional mouse models demonstrate oncogene-dependent differences in tumor maintenance and recurrence. Breast Cancer Res 5(4):202-5. [PubMed: 12817992]  [MGI Ref ID J:84503]

Turnbull L; Zhou HZ; Swigart PM; Turcato S; Karliner JS; Conklin BR; Simpson PC; Baker AJ. 2006. Sustained preconditioning induced by cardiac transgenesis with the tetracycline transactivator. Am J Physiol Heart Circ Physiol 290(3):H1103-9. [PubMed: 16243914]  [MGI Ref ID J:106717]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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