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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Tg(tTAhCMV)3Bjd/J (Changed: 01-AUG-11 ) STOCK Tg(tTAhCMV)3Uh/J (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation B6N1F20 +F1p
Generation DefinitionsDonating Investigator Dr. Hermann Bujard, Universität Heidelberg Appearance
albino
Related Genotype: Tyrc/Tyrc
black
Related Genotype: a/aDescription
These mice were imported to The Jackson Laboratory in 1998. In 2005-2006, we confirmed that these mice express little or no tetracycline-controlled transactivator protein (tTA), even in the absence of doxycycline (dox). A similar conclusion was reported in a recent publication (Frugier et al. 2005. Genesis 42:1-5). The phenotype described below is base on the description of the mice in the original publication (Kistner et al, Proc Natl Acad Sci USA 1996 93:10933-8):STOCK Tg(tTAhCMV)3Bjd/J mice are viable, fertile, and display no overt phenotype. These mice express a tetracycline-controlled transactivator protein (tTA) driven by the human early cytomegalovirus promoter (PhCMV. When transgenic mice are mated to a strain carrying a luciferase reporter gene coupled to a tetracycline-responsive promoter element (TRE; tetO), luciferase was expressed in multiple organs and tissues where PhCMV was known to be active (e.g. muscle, kidney, thymus, heart, pancreas) in the bitransgenic offspring. Treatment with dox reduced the luciferase activity to background levels in most organs; in some cases, the level of luciferase activity was reduced 105-fold in the presence of dox. STOCK Tg(tTAhCMV)3Bjd/J mice may be mated to strains containing a gene of interest coupled to a TRE to study the effects of expression of the target gene under tissue-specific, dox-inducible regulation. Dox may be administered in the animals' water supply.
Development
These mice were designed to express a tetracycline-controlled transactivator protein (tTA) driven by the human early cytomegalovirus promoter (PhCMV). The strain was generated on an NMRI background and was imported on the NMRI (outbred) background. The strain was mated once to C57BL/6J at The Jackson Laboratory.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of tTA
View Strains carrying other alleles of tTA (43 strains)
004644 NOD.Cg Prkdcscid-Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy/YgyJ 011066 NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy/YgyJGckRolyJ 003273 STOCK Tg(CMV-rtTA)4Bjd/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Genetics Research
Tissue/Cell Markers
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CMV-tTA)3Bjd | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, Hermann Bujard | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | CMV-tTA; Tg(tTAhCMV)3Bjd; Tg(tTAhCMV)3Uh; | ||
| Mutation Made By | Dr. Hermann Bujard, Universität Heidelberg | ||
| Strain of Origin | NMRI | ||
| Site of Expression | Expresses tTA in those tissue where the hCMV minimal promoter is active. tTA expression is detected in muscle, kidney, stomach, thymus, heart and pancreas. (NB: Studies conducted in house in 1996, suggest that these mice express little or no tTA, even in the absence of doxycycline.) | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | hCMV, human cytomegalovirus, human | ||
| General Note | Transgenic mice are viable, fertile, and display no overt phenotype. | ||
| Molecular Note | The transgenic construct contained sequence encoding a doxycycline-responsive tetracycline transactivator (tTA) driven by the human early cytomegalovirus promoter. [MGI Ref ID J:93000] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), Melt Curve Analysis
Tg(tTA), QPCR
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Kistner A; Gossen M; Zimmermann F; Jerecic J; Ullmer C; Lubbert H; Bujard H. 1996. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci U S A 93(20):10933-8. [PubMed: 8855286] [MGI Ref ID J:93000]
Additional References
Tg(CMV-tTA)3Bjd relatedEckenstein FP; McGovern T; Kern D; Deignan J. 2006. Neuronal vulnerability in transgenic mice expressing an inducible dominant-negative FGF receptor. Exp Neurol 198(2):338-49. [PubMed: 16487970] [MGI Ref ID J:107899]
Griffin KJ; Kirschner LS; Matyakhina L; Stergiopoulos S; Robinson-White A; Lenherr S; Weinberg FD; Claflin E; Meoli E; Cho-Chung YS; Stratakis CA. 2004. Down-regulation of regulatory subunit type 1A of protein kinase A leads to endocrine and other tumors. Cancer Res 64(24):8811-5. [PubMed: 15604237] [MGI Ref ID J:94953]
Griffin KJ; Kirschner LS; Matyakhina L; Stergiopoulos SG; Robinson-White A; Lenherr SM; Weinberg FD; Claflin ES; Batista D; Bourdeau I; Voutetakis A; Sandrini F; Meoli EM; Bauer AJ; Cho-Chung YS; Bornstein SR; Carney JA; Stratakis CA. 2004. A transgenic mouse bearing an antisense construct of regulatory subunit type 1A of protein kinase A develops endocrine and other tumours: comparison with Carney complex and other PRKAR1A induced lesions. J Med Genet 41(12):923-31. [PubMed: 15591278] [MGI Ref ID J:95465]
Stanger BZ; Tanaka AJ; Melton DA. 2007. Organ size is limited by the number of embryonic progenitor cells in the pancreas but not the liver. Nature 445(7130):886-91. [PubMed: 17259975] [MGI Ref ID J:118596]
Tilli MT; Furth PA. 2003. Conditional mouse models demonstrate oncogene-dependent differences in tumor maintenance and recurrence. Breast Cancer Res 5(4):202-5. [PubMed: 12817992] [MGI Ref ID J:84503]
Turnbull L; Zhou HZ; Swigart PM; Turcato S; Karliner JS; Conklin BR; Simpson PC; Baker AJ. 2006. Sustained preconditioning induced by cardiac transgenesis with the tetracycline transactivator. Am J Physiol Heart Circ Physiol 290(3):H1103-9. [PubMed: 16243914] [MGI Ref ID J:106717]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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