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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Former Names STOCK Tg(tTAhCMV)3Uh/J (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Hemizygote (Female x Male) 23-DEC-08 Species laboratory mouse Generation N1F27 (11-MAR-09) Donating Investigator Hermann Bujard, Universität Heidelberg Appearance
albino
Related Genotype: Tyrc/Tyrc
black
Related Genotype: a/aDescription
These mice were imported to The Jackson Laboratory in 1998. In 2005-2006, we confirmed that these mice express little or no tetracycline-controlled transactivator protein (tTA), even in the absence of doxycycline (dox). A similar conclusion was reported in a recent publication (Frugier et al. 2005. Genesis 42:1-5). The phenotype described below is base on the description of the mice in the original publication (Kistner et al, Proc Natl Acad Sci USA 1996 93:10933-8):STOCK Tg(tTAhCMV)3Bjd/J mice are viable, fertile, and display no overt phenotype. These mice express a tetracycline-controlled transactivator protein (tTA) driven by the human early cytomegalovirus promoter (PhCMV. When transgenic mice are mated to a strain carrying a luciferase reporter gene coupled to a tetracycline-responsive promoter element (TRE; tetO), luciferase was expressed in multiple organs and tissues where PhCMV was known to be active (e.g. muscle, kidney, thymus, heart, pancreas) in the bitransgenic offspring. Treatment with dox reduced the luciferase activity to background levels in most organs; in some cases, the level of luciferase activity was reduced 105-fold in the presence of dox. STOCK Tg(tTAhCMV)3Bjd/J mice may be mated to strains containing a gene of interest coupled to a TRE to study the effects of expression of the target gene under tissue-specific, dox-inducible regulation. Dox may be administered in the animals' water supply.
Development
These mice were designed to express a tetracycline-controlled transactivator protein (tTA) driven by the human early cytomegalovirus promoter (PhCMV). The strain was generated on an NMRI background and was imported on the NMRI (outbred) background. The strain was mated once to C57BL/6J at The Jackson Laboratory.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of tTA
007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 005964 B6.Cg-Tg(GFAP-tTA)110Pop/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 008284 B6.Cg-Tg(Scg2-tTA)1Jt/J 006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 003563 B6.Cg-Tg(tTALap)5Bjd/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J 010573 B6;SJL-Tg(Prl-tTA)6-5Jek/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 005625 FVB-Tg(Pcp2-tTA)3Horr/J 003170 FVB.Cg-Tg(Myh6-tTA)6Smbf/J 006209 FVB.Cg-Tg(Tal1-tTA)19Dgt/J 005942 FVB/N-Tg(Pf4-tTA/VP16)42Kra/J 004937 NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J 009606 STOCK Tg(Six2-EGFP/cre)1Amc/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J View Strains carrying other alleles of tTA (22 strains)
003273 STOCK Tg(rtTAhCMV)4Bjd/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Genetics Research
Tissue/Cell Markers
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tTAhCMV)3Bjd | ||
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| Allele Name | transgene insertion 3, Hermann Bujard | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | CMV-tTA; Tg(tTAhCMV)3Uh; | ||
| Mutation Made By | Hermann Bujard, Universität Heidelberg | ||
| Strain of Origin | NMRI | ||
| Site of Expression | Expresses tTA in those tissue where the hCMV minimal promoter is active. tTA expression is detected in muscle, kidney, stomach, thymus, heart and pancreas. (NB: Studies conducted in house in 1996, suggest that these mice express little or no tTA, even in the absence of doxycycline.) | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | hCMV, human cytomegalovirus, human | ||
| General Note | Transgenic mice are viable, fertile, and display no overt phenotype. | ||
| Molecular Note | The transgenic construct contained sequence encoding a doxycycline-responsive tetracycline transactivator (tTA) driven by the human early cytomegalovirus promoter. [MGI Ref ID J:93000] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), Melt Curve Analysis
Tg(tTA), QPCR
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Kistner A; Gossen M; Zimmermann F; Jerecic J; Ullmer C; Lubbert H; Bujard H. 1996. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci U S A 93(20):10933-8. [PubMed: 8855286] [MGI Ref ID J:93000]
Additional References
Tg(tTAhCMV)3Bjd relatedEckenstein FP; McGovern T; Kern D; Deignan J. 2006. Neuronal vulnerability in transgenic mice expressing an inducible dominant-negative FGF receptor. Exp Neurol 198(2):338-49. [PubMed: 16487970] [MGI Ref ID J:107899]
Griffin KJ; Kirschner LS; Matyakhina L; Stergiopoulos S; Robinson-White A; Lenherr S; Weinberg FD; Claflin E; Meoli E; Cho-Chung YS; Stratakis CA. 2004. Down-regulation of regulatory subunit type 1A of protein kinase A leads to endocrine and other tumors. Cancer Res 64(24):8811-5. [PubMed: 15604237] [MGI Ref ID J:94953]
Griffin KJ; Kirschner LS; Matyakhina L; Stergiopoulos SG; Robinson-White A; Lenherr SM; Weinberg FD; Claflin ES; Batista D; Bourdeau I; Voutetakis A; Sandrini F; Meoli EM; Bauer AJ; Cho-Chung YS; Bornstein SR; Carney JA; Stratakis CA. 2004. A transgenic mouse bearing an antisense construct of regulatory subunit type 1A of protein kinase A develops endocrine and other tumours: comparison with Carney complex and other PRKAR1A induced lesions. J Med Genet 41(12):923-31. [PubMed: 15591278] [MGI Ref ID J:95465]
Stanger BZ; Tanaka AJ; Melton DA. 2007. Organ size is limited by the number of embryonic progenitor cells in the pancreas but not the liver. Nature 445(7130):886-91. [PubMed: 17259975] [MGI Ref ID J:118596]
Tilli MT; Furth PA. 2003. Conditional mouse models demonstrate oncogene-dependent differences in tumor maintenance and recurrence. Breast Cancer Res 5(4):202-5. [PubMed: 12817992] [MGI Ref ID J:84503]
Turnbull L; Zhou HZ; Swigart PM; Turcato S; Karliner JS; Conklin BR; Simpson PC; Baker AJ. 2006. Sustained preconditioning induced by cardiac transgenesis with the tetracycline transactivator. Am J Physiol Heart Circ Physiol 290(3):H1103-9. [PubMed: 16243914] [MGI Ref ID J:106717]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System +/+ sibling x Hemizygote (Female x Male) 23-DEC-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(tTAhCMV)3Bjd
Pairs /Price (US dollars $) Pair Genotype $286.35 Noncarrier x Hemizygous for Tg(tTAhCMV)3Bjd
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(tTAhCMV)3Bjd
Pairs /Price (US dollars $) Pair Genotype $372.30 Noncarrier x Hemizygous for Tg(tTAhCMV)3Bjd
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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