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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names STOCK Tg(rtTAhCMV)4Bjd/J (Changed: 01-AUG-11 ) STOCK Tg(rtTAhCMV)4Uh/J (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 09-SEP-06 Species laboratory mouse Generation N1F8 (24-DEC-08)
Generation DefinitionsDonating Investigator Hermann Bujard, Universität Heidelberg Appearance
agouti
Related Genotype: A/+
black
Related Genotype: a/a
albino
Related Genotype:Description
Homozygous transgenic mice are viable, fertile, and display no overt phenotype. These mice carry the gene encoding the reverse tetracyline-controlled transactivator protein (rtTA) under the control of a human cytomegalovirus early promoter (PhCMV). When these mice are mated to a strain carrying a luciferase reporter gene coupled to a tetracycline-responsive promoter element (TRE; tetO), luciferase expression in the bitransgenic offspring is induced up to 105-fold by treatment with doxycycline (dox) in organs and tissues where PhCMVis known to be active, (i.e. muscle, kidney, thymus and pancreas). Little to no luciferase expression is induced in tissues where PhCMV is not active (i.e. lung, brain, liver and lymphocytes). These mice may be mated to strains containing a gene of interest coupled to a TRE to study the target gene expression effects under tissue-specific, dox-inducible regulation. Dox may be administered in the animals? water supply.Development
This strain is on a mixed NMRI (outbred), C57BL/6 and DBA/2 background.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of rtTA
View Strains carrying other alleles of rtTA (38 strains)
004644 NOD.Cg Prkdcscid-Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy/YgyJ 011066 NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy/YgyJGckRolyJ 003271 STOCK Tg(CMV-tTA)3Bjd/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CMV-rtTA)4Bjd | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Hermann Bujard | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(rtTAhCMV)4Bjd; Tg(rtTAhCMV)4Uh; | ||
| Mutation Made By | Hermann Bujard, Universität Heidelberg | ||
| Strain of Origin | NMRI | ||
| Site of Expression | Expresses rtTA in those tissue where the hCMV minimal promoter is active. rtTA expression has been detected in muscle, kidney, stomach, thymus, heart and pancreas. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | hCMV, human cytomegalovirus, human | ||
| General Note | Hemizygous transgenic mice are viable, fertile, and display no overt phenotype. | ||
| Molecular Note | The transgene contains the gene encoding the reverse tetracyline-controlled transactivator protein (rtTA) under the control of a human cytomegalovirus early promoter. [MGI Ref ID J:93000] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), Melt Curve Analysis
Tg(tTA), QPCR
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Kistner A; Gossen M; Zimmermann F; Jerecic J; Ullmer C; Lubbert H; Bujard H. 1996. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci U S A 93(20):10933-8. [PubMed: 8855286] [MGI Ref ID J:93000]
Additional References
Cheon SS; Cheah AY; Turley S; Nadesan P; Poon R; Clevers H; Alman BA. 2002. beta-Catenin stabilization dysregulates mesenchymal cell proliferation, motility, and invasiveness and causes aggressive fibromatosis and hyperplastic cutaneous wounds. Proc Natl Acad Sci U S A 99(10):6973-8. [PubMed: 11983872] [MGI Ref ID J:76273]
Tg(CMV-rtTA)4Bjd relatedAleksic T; Baumann B; Wagner M; Adler G; Wirth T; Weber CK. 2007. Cellular immune reaction in the pancreas is induced by constitutively active IkappaB kinase-2. Gut 56(2):227-36. [PubMed: 16870717] [MGI Ref ID J:130547]
Cheon SS; Cheah AY; Turley S; Nadesan P; Poon R; Clevers H; Alman BA. 2002. beta-Catenin stabilization dysregulates mesenchymal cell proliferation, motility, and invasiveness and causes aggressive fibromatosis and hyperplastic cutaneous wounds. Proc Natl Acad Sci U S A 99(10):6973-8. [PubMed: 11983872] [MGI Ref ID J:76273]
Gossen M; Freundlieb S; Bender G; Muller G; Hillen W; Bujard H. 1995. Transcriptional activation by tetracyclines in mammalian cells. Science 268(5218):1766-9. [PubMed: 7792603] [MGI Ref ID J:171809]
McJunkin K; Mazurek A; Premsrirut PK; Zuber J; Dow LE; Simon J; Stillman B; Lowe SW. 2011. Reversible suppression of an essential gene in adult mice using transgenic RNA interference. Proc Natl Acad Sci U S A 108(17):7113-8. [PubMed: 21482754] [MGI Ref ID J:171348]
Sodir NM; Swigart LB; Karnezis AN; Hanahan D; Evan GI; Soucek L. 2011. Endogenous Myc maintains the tumor microenvironment. Genes Dev 25(9):907-16. [PubMed: 21478273] [MGI Ref ID J:171447]
Soucek L; Whitfield J; Martins CP; Finch AJ; Murphy DJ; Sodir NM; Karnezis AN; Swigart LB; Nasi S; Evan GI. 2008. Modelling Myc inhibition as a cancer therapy. Nature 455(7213):679-83. [PubMed: 18716624] [MGI Ref ID J:141029]
Tilli MT; Furth PA. 2003. Conditional mouse models demonstrate oncogene-dependent differences in tumor maintenance and recurrence. Breast Cancer Res 5(4):202-5. [PubMed: 12817992] [MGI Ref ID J:84503]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice are bred together. As this strain is on a mixed genetic background, black, albino and agouti coat colors may appear. Mating System Homozygote x Homozygote (Female x Male) 09-SEP-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $225.00 Female or Male Homozygous for Tg(CMV-rtTA)4Bjd
Pairs /Price (US dollars $) Pair Genotype $450.00 Homozygous for Tg(CMV-rtTA)4Bjd x Homozygous for Tg(CMV-rtTA)4Bjd Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $292.50 Female or Male Homozygous for Tg(CMV-rtTA)4Bjd
Pairs /Price (US dollars $) Pair Genotype $585.00 Homozygous for Tg(CMV-rtTA)4Bjd x Homozygous for Tg(CMV-rtTA)4Bjd Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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