Strain Name: |
STOCK Tg(tetNZL)2Bjd/J |
|---|---|
Stock Number: |
003274 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
STOCK Tg(tetNZL)2Uh/J (Changed: 15-DEC-04
) |
| Genes & Alleles | lacZ; luc; tTA; Tg(tetNZL)2Bjd; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Hermann Bujard, Universität Heidelberg Generation +N1 (07-NOV-04) Appearance
white bellied agouti
Related Genotype: Aw/?
black
Related Genotype: a/aStrain Description
In this strain both the lacZ gene with a nuclear localization signal (NZ) and the luciferase gene (L) are driven by a bidirectional, tetracycline-responsive promoter element (TRE). When mated to a strain expressing the tetracycline-controlled activator proteins tTA or rtTA coupled to tissue-specific promoters, lacZ and luciferase will be expressed in the appropriate tissue in a tetracycline-inducible fashion. According to the donating investigator, the expression of the two reporter genes is biased toward higher levels of lacZ than of luciferase, which, although low, is still measurable.Strain Development
In this transgenic strain, both lacZ with a nuclear localization signal (NZ) and the luciferase gene (L) are driven by a bidirectional, tetracycline-responsive promoter element (TRE).
Gene & Allele Details
| Allele Symbol | Tg(tetNZL)2Bjd | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Hermann Bujard | ||
| Common Name(s) | Tg(tetNZL)2Uh; | ||
| Mutation Made By | Hermann Bujard, Universität Heidelberg | ||
| Strain of Origin | NMRI | ||
| Site of Expression | When mated to a strain expressing the tetracycline-controlled activator proteins tTA or rtTA coupled to a tissue-specific promoter, lacZ and luciferase will be expressed in the appropriate tissue in a tetracycline-inducible fashion. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| General Note | In conjunction with a second transgene expressing the tetracycline-controlled activator proteins tTA or rtTA coupled to a tissue-specific promoter (examples include: Tg(Camk2a-tTA)1Mmay, Tg(Myh6-tTA)6Smbf, and Tg(Nes-rtTA)306Rvs), transgenic mice express lacZ and luciferase in the appropiate tissue in a tetracycline-inducible fashion. | ||
| Molecular Note | In this transgene both the lacZ gene with a nuclear localization signal (NZ) and the luciferase gene (L) are driven by a bidirectional, tetracycline-responsive promoter element (TRE). [MGI Ref ID J:93001] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Luciferase: Tg(tetNZL)2Bjd, Tg(tetL)1Bjd, Tg(Fos-luc)1Rnd
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
006102 B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J 006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 005999 B6(SJL)-Tg(SBE/Tk-luc)7Twc/J 003479 B6.C3-Tg(Fos-luc)1Rnd/J 006098 B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J 006101 B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J 002060 FVB/N-Tg(HIV-luc)326Morr/J 003275 STOCK Tg(tetL)1Bjd/J
007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 005964 B6.Cg-Tg(GFAP-tTA)110Pop/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 003563 B6.Cg-Tg(tTALap)5Bjd/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 005625 FVB-Tg(Pcp2-tTA)3Horr/J 003170 FVB.Cg-Tg(Myh6-tTA)6Smbf/J 006209 FVB.Cg-Tg(Tal1-tTA)19Dgt/J 005942 FVB/N-Tg(Pf4-tTA/VP16)42Kra/J 004937 NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J 003272 STOCK Tg(tTALap)5Bjd/J 003271 STOCK Tg(tTAhCMV)3Bjd/J 003275 STOCK Tg(tetL)1Bjd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Research Applications
This mouse can be used to support research in many areas including:
lacZ relatedResearch Tools
lacZ Expression
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Genetics Research (Tissue/Cell Markers: multiple)
Reproductive Biology Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Responsive Strains)
Tet Expression Systems
Research Tools
lacZ Expression
References
Selected Reference(s)
Additional ReferencesBaron U; Freundlieb S; Gossen M; Bujard H. 1995. Co-regulation of two gene activities by tetracycline via a bidirectional promoter. Nucleic Acids Res 23(17):3605-6. [PubMed: 7567477] [MGI Ref ID J:93001]
Price and Supply Information
| Strain Name: | STOCK Tg(tetNZL)2Bjd/J |
| Stock Number: | 003274 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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