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Strain Name:

STOCK Tg(tetL)1Bjd/J

Stock Number:

003275

Availability:

Repository-Cryopreserved

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Additional Licensing and Use Restrictions

Former Name      STOCK Tg(tetL)1Uh/J    (Changed: 15-DEC-04 )
Genes & Alleles   luc;   tTA;   Tg(tetL)1Bjd;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Hermann Bujard,   Universität Heidelberg
GenerationN2F8pN1

Appearance
black
Related Genotype: a/a

Strain Description
STOCK Tg(tetL)1Bjd/J transgenic mice are viable, fertile, and do not display an overt phenotype. These transgenic mice carry the luciferase gene coupled to a tetracycline-responsive promoter element (TRE). When mated to a strain expressing the tetracycline-controlled activator proteins tTA or rtTA coupled to a tissue-specific promoter, luciferase will be expressed in the appropriate tissue in a tetracycline-inducible fashion.

Strain Development
The strain originated on an NMRI (outbred) background and remains on this background.

Gene & Allele Details

Allele Symbol Tg(tetL)1Bjd
Allele Name transgene insertion 1, Hermann Bujard
Common Name(s) Tg(tetL)1Uh;
Mutation Made By Hermann Bujard,   Universität Heidelberg
Strain of OriginNMRI
Site of ExpressionWhen mated to a strain carrying the tTA gene coupled to a tissue specific promoter, luciferase will be expressed in the appropriate tissue.
Expressed Gene luc, luciferase, firefly
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
General Note Transgenic mice are viable, fertile, and do not display an overt phenotype.

In conjunction with a second transgene expressing the tetracycline-controlled activator proteins tTA or rtTA coupled to a tissue-specific promoter (examples include: Tg(Camk2a-tTA)1Mmay, Tg(Myh6-tTA)6Smbf, and Tg(Nes-rtTA)306Rvs), transgenic mice express luciferase in the appropiate tissue in a tetracycline-inducible fashion.

Molecular Note The transgene carries the luciferase gene coupled to a tetracycline-responsive promoter element (TRE). [MGI Ref ID J:93000]

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Genotyping Protocols

Luciferase: Tg(tetNZL)2Bjd, Tg(tetL)1Bjd, Tg(Fos-luc)1Rnd

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of luc     (10 strains)

View Strains carrying other alleles of tTA     (19 strains)

Additional Web Information

Tet Expression Systems

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Reproductive Biology Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Responsive Strains)

References

Selected Reference(s)

Kistner A; Gossen M; Zimmermann F; Jerecic J; Ullmer C; Lubbert H; Bujard H. 1996. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci U S A 93(20):10933-8. [PubMed: 8855286]  [MGI Ref ID J:93000]

Additional References

Price and Supply Information

Strain Name: STOCK Tg(tetL)1Bjd/J
Stock Number: 003275

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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