Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Dr. T. R. Kumar,   University of Kansas

Appearance
white-bellied agouti
Related Genotype: A^w/?

black
Related Genotype: a/a

Description
Males carrying the targeted mutation are fertile, have small testes, reduced sperm number and sperm motility. Homozygous mutant females are infertile due to a preantral stage block in ovarian folliculogenesis. Heterozygotes are fertile.

Control Information

	Control
	Wild-type from the colony
	100903 B6129PF2/J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Follicle-Stimulating Hormone Deficiency, Isolated   (FSHB)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Fshb^tm1Zuk/Fshb^tm1Zuk

        either: 129S/SvEv or (involves: 129S7/SvEvBrd * C57BL/6)

• endocrine/exocrine gland phenotype
• absent corpus luteum   (MGI Ref ID J:38099)
• absent mature ovarian follicles   (MGI Ref ID J:38099)
• impaired ovarian folliculogenesis
  • blocked prior to antral follicle formation   (MGI Ref ID J:38099)
• small ovary   (MGI Ref ID J:38099)
• small seminiferous tubules   (MGI Ref ID J:38099)
• small testis
  • progressive reduction in size beginning at 14 days of age   (MGI Ref ID J:38099)
  • however, serum testosterone levels in males were similar to those of wild-type and the epididymides and seminal glands were of normal size   (MGI Ref ID J:38099)
• homeostasis/metabolism phenotype
• decreased circulating progesterone level
  • 50% decrease relative to wild-type   (MGI Ref ID J:38099)
• decreased follicle stimulating hormone level
  • FSH was undetected in the pituitary   (MGI Ref ID J:38099)
• • decreased circulating follicle stimulating hormone level
    • FSH was undetected in serum   (MGI Ref ID J:38099)
• increased circulating luteinizing hormone level
  • elevated only in females   (MGI Ref ID J:38099)
• reproductive system phenotype
• abnormal estrous cycle   (MGI Ref ID J:38099)
• absent corpus luteum   (MGI Ref ID J:38099)
• absent mature ovarian follicles   (MGI Ref ID J:38099)
• asthenozoospermia
  • 40% reduction in the number of motile sperm relative to wild-type   (MGI Ref ID J:38099)
• female infertility
  • females but not males are infertile   (MGI Ref ID J:38099)
• impaired ovarian folliculogenesis
  • blocked prior to antral follicle formation   (MGI Ref ID J:38099)
• oligozoospermia
  • 75% reduction of epididymal sperm count relative to wild-type   (MGI Ref ID J:38099)
• small ovary   (MGI Ref ID J:38099)
• small seminiferous tubules   (MGI Ref ID J:38099)
• small testis
  • progressive reduction in size beginning at 14 days of age   (MGI Ref ID J:38099)
  • however, serum testosterone levels in males were similar to those of wild-type and the epididymides and seminal glands were of normal size   (MGI Ref ID J:38099)
• small uterus
  • markedly thinner than wild-type   (MGI Ref ID J:38099)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Fshb^tm1Zuk/Fshb^tm1Zuk

        involves: 129S7/SvEvBrd

• endocrine/exocrine gland phenotype
• abnormal Sertoli cell morphology
  • 30% decrease in the number of sertoli cells relative to wild-type   (MGI Ref ID J:71932)
• decreased testis weight
  • 60% reduction in weight relative to wild-type   (MGI Ref ID J:71932)
• reproductive system phenotype
• abnormal Sertoli cell morphology
  • 30% decrease in the number of sertoli cells relative to wild-type   (MGI Ref ID J:71932)
• abnormal spermatogenesis
  • lower Sertoli cell to round spermatid ratio   (MGI Ref ID J:71932)
  • decrease in the ratios of spermatogonia and early stages of the first meiotic prophase (preleptotene, leptotene, and zygotene) to the later stages of meiotic prophase (pachytene and diplotene) and of pachytene/diplotene spermatocyte to round round spermatids, indicating impaired spermatogenesis   (MGI Ref ID J:71932)
• • abnormal spermatid morphology
    • 60% reduction in round spermatids   (MGI Ref ID J:71932)
• abnormal spermatogonia morphology
  • 46% reduction in spermatogonia   (MGI Ref ID J:71932)
• decreased male germ cell number
  • decline in germ cell numbers that becomes more marked as spermatogenesis progresses; 46% reduction in A and I spermatogonia and a 60% reduction in round spermatids   (MGI Ref ID J:71932)
• decreased testis weight
  • 60% reduction in weight relative to wild-type   (MGI Ref ID J:71932)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Fshb^tm1Zuk related

Endocrine Deficiency Research
Gonad Defects
Hypothalamus/Pituitary Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
Endocrine Deficiencies Affecting Gonads
Fertility Defects
      females only

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Fshbtm1Zuk
Allele Name		targeted mutation 1, Martin M Matzuk
Allele Type		Targeted (knock-out)
Common Name(s)		FSHbeta-; FSHbetaKO; fshbm1;
Mutation Made By		Dr. T. Kumar,   University of Kansas
Strain of Origin		129S7/SvEvBrd-Hprt
ES Cell Line Name		AB2.1
ES Cell Line Strain		129S7/SvEvBrd-Hprt
Gene Symbol and Name		Fshb, follicle stimulating hormone beta
Chromosome		2
Gene Common Name(s)		FSH; Fshbeta;
Molecular Note		An HPRT minigene replaced a genomic fragment containing exons 1, 2 and part of 3. Northern blot analysis on RNA derived from pituitary of homozygous mice demonstrated that no detectable transcript was produced from thia allele. Radioimmunoasays on serum from homozygous mice confirmed that no functional protein was expressed from this allele. [MGI Ref ID J:38099]

Genotyping

Genotyping Information

Genotyping Protocols

Fshb^tm1Zuk, Standard PCR
Human HPRT Melt Curve Analysis, Melt Curve Analysis
Human HPRT, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kumar TR; Wang Y; Lu N; Matzuk MM. 1997. Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. Nat Genet 15(2):201-4. [PubMed: 9020850]  [MGI Ref ID J:38099]

Additional References

Fshb^tm1Zuk related

Abel MH; Huhtaniemi I; Pakarinen P; Kumar TR; Charlton HM. 2003. Age-related uterine and ovarian hypertrophy in FSH receptor knockout and FSHbeta subunit knockout mice. Reproduction 125(2):165-73. [PubMed: 12578530]  [MGI Ref ID J:81519]

Baker PJ; Pakarinen P; Huhtaniemi IT; Abel MH; Charlton HM; Kumar TR; O'Shaughnessy PJ. 2003. Failure of normal Leydig cell development in follicle-stimulating hormone (FSH) receptor-deficient mice, but not FSHbeta-deficient mice: role for constitutive FSH receptor activity. Endocrinology 144(1):138-45. [PubMed: 12488339]  [MGI Ref ID J:81408]

Bolden-Tiller OU; Chiarini-Garcia H; Poirier C; Alves-Freitas D; Weng CC; Shetty G; Meistrich ML. 2007. Genetic factors contributing to defective spermatogonial differentiation in juvenile spermatogonial depletion (Utp14bjsd) mice. Biol Reprod 77(2):237-46. [PubMed: 17475932]  [MGI Ref ID J:124196]

Burns KH; Owens GE; Ogbonna SC; Nilson JH; Matzuk MM. 2003. Expression profiling analyses of gonadotropin responses and tumor development in the absence of inhibins. Endocrinology 144(10):4492-507. [PubMed: 12959983]  [MGI Ref ID J:85767]

Burns KH; Yan C; Kumar TR; Matzuk MM. 2001. Analysis of ovarian gene expression in follicle-stimulating hormone beta knockout mice. Endocrinology 142(7):2742-51. [PubMed: 11415992]  [MGI Ref ID J:71936]

Choi Y; Rajkovic A. 2006. Genetics of early mammalian folliculogenesis. Cell Mol Life Sci 63(5):579-90. [PubMed: 16416028]  [MGI Ref ID J:108336]

Combelles CM; Carabatsos MJ; Kumar TR; Matzuk MM; Albertini DF. 2004. Hormonal control of somatic cell oocyte interactions during ovarian follicle development. Mol Reprod Dev 69(3):347-55. [PubMed: 15349847]  [MGI Ref ID J:95552]

Demeestere I; Streiff AK; Suzuki J; Al-Khabouri S; Mahrous E; Tan SL; Clarke HJ. 2012. Follicle-stimulating hormone accelerates mouse oocyte development in vivo. Biol Reprod 87(1):3, 1-11. [PubMed: 22517620]  [MGI Ref ID J:192381]

Garcia-Campayo V; Boime I; Ma X; Daphna-Iken D; Kumar TR. 2005. A single-chain tetradomain glycoprotein hormone analog elicits multiple hormone activities in vivo. Biol Reprod 72(2):301-8. [PubMed: 15385421]  [MGI Ref ID J:115456]

Hirst RC; Abel MH; Wilkins V; Simpson C; Knight PG; Zhang FP; Huhtaniemi I; Kumar TR; Charlton HM. 2004. Influence of mutations affecting gonadotropin production or responsiveness on expression of inhibin subunit mRNA and protein in the mouse ovary. Reproduction 128(1):43-52. [PubMed: 15232063]  [MGI Ref ID J:91232]

Iqbal J; Sun L; Kumar TR; Blair HC; Zaidi M. 2006. Follicle-stimulating hormone stimulates TNF production from immune cells to enhance osteoblast and osteoclast formation. Proc Natl Acad Sci U S A 103(40):14925-30. [PubMed: 17003115]  [MGI Ref ID J:114693]

Johnston H; Baker PJ; Abel M; Charlton HM; Jackson G; Fleming L; Kumar TR; O'Shaughnessy PJ. 2004. Regulation of Sertoli cell number and activity by follicle-stimulating hormone and androgen during postnatal development in the mouse. Endocrinology 145(1):318-29. [PubMed: 14551232]  [MGI Ref ID J:87442]

Kumar TR; Palapattu G; Wang P; Woodruff TK; Boime I; Byrne MC ; Matzuk MM. 1999. Transgenic models to study gonadotropin function: the role of follicle-stimulating hormone in gonadal growth and tumorigenesis. Mol Endocrinol 13(6):851-65. [PubMed: 10379885]  [MGI Ref ID J:55802]

Kumar TR; Varani S; Wreford NG; Telfer NM; de Kretser DM; Matzuk MM. 2001. Male reproductive phenotypes in double mutant mice lacking both FSHbeta and activin receptor IIA. Endocrinology 142(8):3512-8. [PubMed: 11459797]  [MGI Ref ID J:108808]

Lukas-Croisier C; Lasala C; Nicaud J; Bedecarras P; Kumar TR; Dutertre M; Matzuk MM; Picard JY; Josso N; Rey R. 2003. Follicle-Stimulating Hormone Increases Testicular Anti-Mullerian Hormone (AMH) Production through Sertoli Cell Proliferation and a Nonclassical Cyclic Adenosine 5'-Monophosphate-Mediated Activation of the AMH Gene. Mol Endocrinol 17(4):550-61. [PubMed: 12554789]  [MGI Ref ID J:82678]

Sun L; Peng Y; Sharrow AC; Iqbal J; Zhang Z; Papachristou DJ; Zaidi S; Zhu LL; Yaroslavskiy BB; Zhou H; Zallone A; Sairam MR; Kumar TR; Bo W; Braun J; Cardoso-Landa L; Schaffler MB; Moonga BS; Blair HC; Zaidi M. 2006. FSH directly regulates bone mass. Cell 125(2):247-60. [PubMed: 16630814]  [MGI Ref ID J:115870]

Trousdale RK; Wolgemuth DJ. 2004. Bromodomain containing 2 (Brd2) is expressed in distinct patterns during ovarian folliculogenesis independent of FSH or GDF9 action. Mol Reprod Dev 68(3):261-8. [PubMed: 15112318]  [MGI Ref ID J:109752]

Wreford NG; Rajendra Kumar T; Matzuk MM; de Kretser DM. 2001. Analysis of the testicular phenotype of the follicle-stimulating hormone beta-subunit knockout and the activin type II receptor knockout mice by stereological analysis. Endocrinology 142(7):2916-20. [PubMed: 11416011]  [MGI Ref ID J:71932]

Zhou J; Kumar TR; Matzuk MM; Bondy C. 1997. Insulin-like growth factor I regulates gonadotropin responsiveness in the murine ovary. Mol Endocrinol 11(13):1924-33. [PubMed: 9415397]  [MGI Ref ID J:105227]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
	100903 B6129PF2/J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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JAX^® Mice
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Terms of Use

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General Terms and Conditions

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