Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator T. Kumar,   University of Kansas

Appearance
white-bellied agouti
Related Genotype: A^w/?

black
Related Genotype: a/a

Description
Males carrying the targeted mutation are fertile, have small testes, reduced sperm number and sperm motility. Homozygous mutant females are infertile due to a preantral stage block in ovarian folliculogenesis. Heterozygotes are fertile.

Control Information

	Control
	Wild-type from the colony
	100903 B6129PF2/J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Fshb^tm1Zuk/Fshb^tm1Zuk

        either: 129S/SvEv or (involves: 129S7/SvEvBrd * C57BL/6)

• endocrine/exocrine gland phenotype
• absent corpus luteum (MGI Ref ID J:38099)
• absent mature ovarian follicles (MGI Ref ID J:38099)
• impaired ovarian folliculogenesis (MGI Ref ID J:38099)
  • blocked prior to antral follicle formation
• small ovary (MGI Ref ID J:38099)
• small seminiferous tubules (MGI Ref ID J:38099)
• small testis (MGI Ref ID J:38099)
  • progressive reduction in size beginning at 14 days of age
  • however, serum testosterone levels in males were similar to those of wild-type and the epididymides and seminal glands were of normal size
• homeostasis/metabolism phenotype
• decreased circulating progesterone level (MGI Ref ID J:38099)
  • 50% decrease relative to wild-type
• decreased follicle stimulating hormone level (MGI Ref ID J:38099)
  • FSH was undetected in the pituitary
• decreased circulating follicle stimulating hormone level (MGI Ref ID J:38099)
  • FSH was undetected in serum
• increased circulating luteinizing hormone level (MGI Ref ID J:38099)
  • elevated only in females
• reproductive system phenotype
• abnormal estrous cycle (MGI Ref ID J:38099)
• absent corpus luteum (MGI Ref ID J:38099)
• absent mature ovarian follicles (MGI Ref ID J:38099)
• asthenozoospermia (MGI Ref ID J:38099)
  • 40% reduction in the number of motile sperm relative to wild-type
• female infertility (MGI Ref ID J:38099)
  • females but not males are infertile
• impaired ovarian folliculogenesis (MGI Ref ID J:38099)
  • blocked prior to antral follicle formation
• oligozoospermia (MGI Ref ID J:38099)
  • 75% reduction of epididymal sperm count relative to wild-type
• small ovary (MGI Ref ID J:38099)
• small seminiferous tubules (MGI Ref ID J:38099)
• small testis (MGI Ref ID J:38099)
  • progressive reduction in size beginning at 14 days of age
  • however, serum testosterone levels in males were similar to those of wild-type and the epididymides and seminal glands were of normal size
• small uterus (MGI Ref ID J:38099)
  • markedly thinner than wild-type

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Fshb^tm1Zuk/Fshb^tm1Zuk

        Background Not Specified

• endocrine/exocrine gland phenotype
• abnormal Sertoli cell morphology (MGI Ref ID J:71932)
  • 30 to 39% decrease in the number of sertoli cells relative to wild-type
• decreased testis weight (MGI Ref ID J:71932)
  • 60% reduction in weight relative to wild-type
• reproductive system phenotype
• abnormal Sertoli cell morphology (MGI Ref ID J:71932)
  • 30 to 39% decrease in the number of sertoli cells relative to wild-type
• decreased male germ cell number (MGI Ref ID J:71932)
  • reductions in the number of spermatogonia and round spermatids
• decreased testis weight (MGI Ref ID J:71932)
  • 60% reduction in weight relative to wild-type

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Fshb^tm1Zuk related

Endocrine Deficiency Research
Gonad Defects
Hypothalamus/Pituitary Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
Endocrine Deficiencies Affecting Gonads
Fertility Defects (females only)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Fshbtm1Zuk
Allele Name		targeted mutation 1, Martin M Matzuk
Allele Type		Targeted (knock-out)
Common Name(s)		FSHbeta-; FSHbetaKO; fshbm1;
Mutation Made By		T. Kumar,   University of Kansas
Strain of Origin		129S7/SvEvBrd-Hprt1b-m2
ES Cell Line Name		AB2.1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1
Gene Symbol and Name		Fshb, follicle stimulating hormone beta
Chromosome		2
Gene Common Name(s)		Fshbeta;
Molecular Note		An HPRT minigene replaced a genomic fragment containing exons 1, 2 and part of 3. Northern blot analysis on RNA derived from pituitary of homozygous mice demonstrated that no detectable transcript was produced from thia allele. Radioimmunoasays on serum from homozygous mice confirmed that no functional protein was expressed from this allele. [MGI Ref ID J:38099]

Genotyping

Genotyping Information

Genotyping Protocols

Fshb^tm1Zuk, STD PCR, vers. 1
Human HPRT, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Kumar TR; Wang Y; Lu N; Matzuk MM. 1997. Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. Nat Genet 15(2):201-4. [PubMed: 9020850]  [MGI Ref ID J:38099]

Additional References

Fshb^tm1Zuk related

Abel MH; Huhtaniemi I; Pakarinen P; Kumar TR; Charlton HM. 2003. Age-related uterine and ovarian hypertrophy in FSH receptor knockout and FSHbeta subunit knockout mice. Reproduction 125(2):165-73. [PubMed: 12578530]  [MGI Ref ID J:81519]

Baker PJ; Pakarinen P; Huhtaniemi IT; Abel MH; Charlton HM; Kumar TR; O'Shaughnessy PJ. 2003. Failure of normal Leydig cell development in follicle-stimulating hormone (FSH) receptor-deficient mice, but not FSHbeta-deficient mice: role for constitutive FSH receptor activity. Endocrinology 144(1):138-45. [PubMed: 12488339]  [MGI Ref ID J:81408]

Bolden-Tiller OU; Chiarini-Garcia H; Poirier C; Alves-Freitas D; Weng CC; Shetty G; Meistrich ML. 2007. Genetic factors contributing to defective spermatogonial differentiation in juvenile spermatogonial depletion (Utp14bjsd) mice. Biol Reprod 77(2):237-46. [PubMed: 17475932]  [MGI Ref ID J:124196]

Burns KH; Owens GE; Ogbonna SC; Nilson JH; Matzuk MM. 2003. Expression profiling analyses of gonadotropin responses and tumor development in the absence of inhibins. Endocrinology 144(10):4492-507. [PubMed: 12959983]  [MGI Ref ID J:85767]

Burns KH; Yan C; Kumar TR; Matzuk MM. 2001. Analysis of ovarian gene expression in follicle-stimulating hormone beta knockout mice. Endocrinology 142(7):2742-51. [PubMed: 11415992]  [MGI Ref ID J:71936]

Choi Y; Rajkovic A. 2006. Genetics of early mammalian folliculogenesis. Cell Mol Life Sci 63(5):579-90. [PubMed: 16416028]  [MGI Ref ID J:108336]

Combelles CM; Carabatsos MJ; Kumar TR; Matzuk MM; Albertini DF. 2004. Hormonal control of somatic cell oocyte interactions during ovarian follicle development. Mol Reprod Dev 69(3):347-55. [PubMed: 15349847]  [MGI Ref ID J:95552]

Garcia-Campayo V; Boime I; Ma X; Daphna-Iken D; Kumar TR. 2005. A single-chain tetradomain glycoprotein hormone analog elicits multiple hormone activities in vivo. Biol Reprod 72(2):301-8. [PubMed: 15385421]  [MGI Ref ID J:115456]

Hirst RC; Abel MH; Wilkins V; Simpson C; Knight PG; Zhang FP; Huhtaniemi I; Kumar TR; Charlton HM. 2004. Influence of mutations affecting gonadotropin production or responsiveness on expression of inhibin subunit mRNA and protein in the mouse ovary. Reproduction 128(1):43-52. [PubMed: 15232063]  [MGI Ref ID J:91232]

Iqbal J; Sun L; Kumar TR; Blair HC; Zaidi M. 2006. Follicle-stimulating hormone stimulates TNF production from immune cells to enhance osteoblast and osteoclast formation. Proc Natl Acad Sci U S A 103(40):14925-30. [PubMed: 17003115]  [MGI Ref ID J:114693]

Johnston H; Baker PJ; Abel M; Charlton HM; Jackson G; Fleming L; Kumar TR; O'Shaughnessy PJ. 2004. Regulation of Sertoli cell number and activity by follicle-stimulating hormone and androgen during postnatal development in the mouse. Endocrinology 145(1):318-29. [PubMed: 14551232]  [MGI Ref ID J:87442]

Kumar TR; Palapattu G; Wang P; Woodruff TK; Boime I; Byrne MC ; Matzuk MM. 1999. Transgenic models to study gonadotropin function: the role of follicle-stimulating hormone in gonadal growth and tumorigenesis. Mol Endocrinol 13(6):851-65. [PubMed: 10379885]  [MGI Ref ID J:55802]

Kumar TR; Varani S; Wreford NG; Telfer NM; de Kretser DM; Matzuk MM. 2001. Male reproductive phenotypes in double mutant mice lacking both FSHbeta and activin receptor IIA. Endocrinology 142(8):3512-8. [PubMed: 11459797]  [MGI Ref ID J:108808]

Lukas-Croisier C; Lasala C; Nicaud J; Bedecarras P; Kumar TR; Dutertre M; Matzuk MM; Picard JY; Josso N; Rey R. 2003. Follicle-Stimulating Hormone Increases Testicular Anti-Mullerian Hormone (AMH) Production through Sertoli Cell Proliferation and a Nonclassical Cyclic Adenosine 5'-Monophosphate-Mediated Activation of the AMH Gene. Mol Endocrinol 17(4):550-61. [PubMed: 12554789]  [MGI Ref ID J:82678]

Sun L; Peng Y; Sharrow AC; Iqbal J; Zhang Z; Papachristou DJ; Zaidi S; Zhu LL; Yaroslavskiy BB; Zhou H; Zallone A; Sairam MR; Kumar TR; Bo W; Braun J; Cardoso-Landa L; Schaffler MB; Moonga BS; Blair HC; Zaidi M. 2006. FSH directly regulates bone mass. Cell 125(2):247-60. [PubMed: 16630814]  [MGI Ref ID J:115870]

Trousdale RK; Wolgemuth DJ. 2004. Bromodomain containing 2 (Brd2) is expressed in distinct patterns during ovarian folliculogenesis independent of FSH or GDF9 action. Mol Reprod Dev 68(3):261-8. [PubMed: 15112318]  [MGI Ref ID J:109752]

Wreford NG; Rajendra Kumar T; Matzuk MM; de Kretser DM. 2001. Analysis of the testicular phenotype of the follicle-stimulating hormone beta-subunit knockout and the activin type II receptor knockout mice by stereological analysis. Endocrinology 142(7):2916-20. [PubMed: 11416011]  [MGI Ref ID J:71932]

Zhou J; Kumar TR; Matzuk MM; Bondy C. 1997. Insulin-like growth factor I regulates gonadotropin responsiveness in the murine ovary. Mol Endocrinol 11(13):1924-33. [PubMed: 9415397]  [MGI Ref ID J:105227]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
	100903 B6129PF2/J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.