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Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator T. Kumar, University of Kansas Appearance
white-bellied agouti
Related Genotype: Aw/?
black
Related Genotype: a/aDescription
Males carrying the targeted mutation are fertile, have small testes, reduced sperm number and sperm motility. Homozygous mutant females are infertile due to a preantral stage block in ovarian folliculogenesis. Heterozygotes are fertile.
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 100903 B6129PF2/J | ||
| Considerations for Choosing Controls | ||
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
Fshbtm1Zuk/Fshbtm1Zuk
either: 129S/SvEv or (involves: 129S7/SvEvBrd * C57BL/6)
- endocrine/exocrine gland phenotype
- absent corpus luteum (MGI Ref ID J:38099)
- absent mature ovarian follicles (MGI Ref ID J:38099)
- impaired ovarian folliculogenesis (MGI Ref ID J:38099)
- blocked prior to antral follicle formation
- small ovary (MGI Ref ID J:38099)
- small seminiferous tubules (MGI Ref ID J:38099)
- small testis (MGI Ref ID J:38099)
- progressive reduction in size beginning at 14 days of age
- however, serum testosterone levels in males were similar to those of wild-type and the epididymides and seminal glands were of normal size
- homeostasis/metabolism phenotype
- decreased circulating progesterone level (MGI Ref ID J:38099)
- 50% decrease relative to wild-type
- decreased follicle stimulating hormone level (MGI Ref ID J:38099)
- FSH was undetected in the pituitary
- decreased circulating follicle stimulating hormone level (MGI Ref ID J:38099)
- FSH was undetected in serum
- increased circulating luteinizing hormone level (MGI Ref ID J:38099)
- elevated only in females
- reproductive system phenotype
- abnormal estrous cycle (MGI Ref ID J:38099)
- absent corpus luteum (MGI Ref ID J:38099)
- absent mature ovarian follicles (MGI Ref ID J:38099)
- asthenozoospermia (MGI Ref ID J:38099)
- 40% reduction in the number of motile sperm relative to wild-type
- female infertility (MGI Ref ID J:38099)
- females but not males are infertile
- impaired ovarian folliculogenesis (MGI Ref ID J:38099)
- blocked prior to antral follicle formation
- oligozoospermia (MGI Ref ID J:38099)
- 75% reduction of epididymal sperm count relative to wild-type
- small ovary (MGI Ref ID J:38099)
- small seminiferous tubules (MGI Ref ID J:38099)
- small testis (MGI Ref ID J:38099)
- progressive reduction in size beginning at 14 days of age
- however, serum testosterone levels in males were similar to those of wild-type and the epididymides and seminal glands were of normal size
- small uterus (MGI Ref ID J:38099)
- markedly thinner than wild-type
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Fshbtm1Zuk/Fshbtm1Zuk
Background Not Specified
- endocrine/exocrine gland phenotype
- abnormal Sertoli cell morphology (MGI Ref ID J:71932)
- 30 to 39% decrease in the number of sertoli cells relative to wild-type
- decreased testis weight (MGI Ref ID J:71932)
- 60% reduction in weight relative to wild-type
- reproductive system phenotype
- abnormal Sertoli cell morphology (MGI Ref ID J:71932)
- 30 to 39% decrease in the number of sertoli cells relative to wild-type
- decreased male germ cell number (MGI Ref ID J:71932)
- reductions in the number of spermatogonia and round spermatids
- decreased testis weight (MGI Ref ID J:71932)
- 60% reduction in weight relative to wild-type
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:Fshbtm1Zuk related
Endocrine Deficiency Research
Gonad Defects
Hypothalamus/Pituitary Defects
Reproductive Biology Research
Developmental Defects Affecting Gonads
Endocrine Deficiencies Affecting Gonads
Fertility Defects (females only)
| Allele Symbol | Fshbtm1Zuk | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Martin M Matzuk | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | FSHbeta-; FSHbetaKO; fshbm1; | ||
| Mutation Made By | T. Kumar, University of Kansas | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1b-m2 | ||
| ES Cell Line Name | AB2.1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1 | ||
| Gene Symbol and Name | Fshb, follicle stimulating hormone beta | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | Fshbeta; | ||
| Molecular Note | An HPRT minigene replaced a genomic fragment containing exons 1, 2 and part of 3. Northern blot analysis on RNA derived from pituitary of homozygous mice demonstrated that no detectable transcript was produced from thia allele. Radioimmunoasays on serum from homozygous mice confirmed that no functional protein was expressed from this allele. [MGI Ref ID J:38099] | ||
Genotyping Protocols
Fshbtm1Zuk, STD PCR, vers. 1
Human HPRT, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
Kumar TR; Wang Y; Lu N; Matzuk MM. 1997. Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. Nat Genet 15(2):201-4. [PubMed: 9020850] [MGI Ref ID J:38099]
Fshbtm1Zuk relatedAbel MH; Huhtaniemi I; Pakarinen P; Kumar TR; Charlton HM. 2003. Age-related uterine and ovarian hypertrophy in FSH receptor knockout and FSHbeta subunit knockout mice. Reproduction 125(2):165-73. [PubMed: 12578530] [MGI Ref ID J:81519]
Baker PJ; Pakarinen P; Huhtaniemi IT; Abel MH; Charlton HM; Kumar TR; O'Shaughnessy PJ. 2003. Failure of normal Leydig cell development in follicle-stimulating hormone (FSH) receptor-deficient mice, but not FSHbeta-deficient mice: role for constitutive FSH receptor activity. Endocrinology 144(1):138-45. [PubMed: 12488339] [MGI Ref ID J:81408]
Bolden-Tiller OU; Chiarini-Garcia H; Poirier C; Alves-Freitas D; Weng CC; Shetty G; Meistrich ML. 2007. Genetic factors contributing to defective spermatogonial differentiation in juvenile spermatogonial depletion (Utp14bjsd) mice. Biol Reprod 77(2):237-46. [PubMed: 17475932] [MGI Ref ID J:124196]
Burns KH; Owens GE; Ogbonna SC; Nilson JH; Matzuk MM. 2003. Expression profiling analyses of gonadotropin responses and tumor development in the absence of inhibins. Endocrinology 144(10):4492-507. [PubMed: 12959983] [MGI Ref ID J:85767]
Burns KH; Yan C; Kumar TR; Matzuk MM. 2001. Analysis of ovarian gene expression in follicle-stimulating hormone beta knockout mice. Endocrinology 142(7):2742-51. [PubMed: 11415992] [MGI Ref ID J:71936]
Choi Y; Rajkovic A. 2006. Genetics of early mammalian folliculogenesis. Cell Mol Life Sci 63(5):579-90. [PubMed: 16416028] [MGI Ref ID J:108336]
Combelles CM; Carabatsos MJ; Kumar TR; Matzuk MM; Albertini DF. 2004. Hormonal control of somatic cell oocyte interactions during ovarian follicle development. Mol Reprod Dev 69(3):347-55. [PubMed: 15349847] [MGI Ref ID J:95552]
Garcia-Campayo V; Boime I; Ma X; Daphna-Iken D; Kumar TR. 2005. A single-chain tetradomain glycoprotein hormone analog elicits multiple hormone activities in vivo. Biol Reprod 72(2):301-8. [PubMed: 15385421] [MGI Ref ID J:115456]
Hirst RC; Abel MH; Wilkins V; Simpson C; Knight PG; Zhang FP; Huhtaniemi I; Kumar TR; Charlton HM. 2004. Influence of mutations affecting gonadotropin production or responsiveness on expression of inhibin subunit mRNA and protein in the mouse ovary. Reproduction 128(1):43-52. [PubMed: 15232063] [MGI Ref ID J:91232]
Iqbal J; Sun L; Kumar TR; Blair HC; Zaidi M. 2006. Follicle-stimulating hormone stimulates TNF production from immune cells to enhance osteoblast and osteoclast formation. Proc Natl Acad Sci U S A 103(40):14925-30. [PubMed: 17003115] [MGI Ref ID J:114693]
Johnston H; Baker PJ; Abel M; Charlton HM; Jackson G; Fleming L; Kumar TR; O'Shaughnessy PJ. 2004. Regulation of Sertoli cell number and activity by follicle-stimulating hormone and androgen during postnatal development in the mouse. Endocrinology 145(1):318-29. [PubMed: 14551232] [MGI Ref ID J:87442]
Kumar TR; Palapattu G; Wang P; Woodruff TK; Boime I; Byrne MC ; Matzuk MM. 1999. Transgenic models to study gonadotropin function: the role of follicle-stimulating hormone in gonadal growth and tumorigenesis. Mol Endocrinol 13(6):851-65. [PubMed: 10379885] [MGI Ref ID J:55802]
Kumar TR; Varani S; Wreford NG; Telfer NM; de Kretser DM; Matzuk MM. 2001. Male reproductive phenotypes in double mutant mice lacking both FSHbeta and activin receptor IIA. Endocrinology 142(8):3512-8. [PubMed: 11459797] [MGI Ref ID J:108808]
Lukas-Croisier C; Lasala C; Nicaud J; Bedecarras P; Kumar TR; Dutertre M; Matzuk MM; Picard JY; Josso N; Rey R. 2003. Follicle-Stimulating Hormone Increases Testicular Anti-Mullerian Hormone (AMH) Production through Sertoli Cell Proliferation and a Nonclassical Cyclic Adenosine 5'-Monophosphate-Mediated Activation of the AMH Gene. Mol Endocrinol 17(4):550-61. [PubMed: 12554789] [MGI Ref ID J:82678]
Sun L; Peng Y; Sharrow AC; Iqbal J; Zhang Z; Papachristou DJ; Zaidi S; Zhu LL; Yaroslavskiy BB; Zhou H; Zallone A; Sairam MR; Kumar TR; Bo W; Braun J; Cardoso-Landa L; Schaffler MB; Moonga BS; Blair HC; Zaidi M. 2006. FSH directly regulates bone mass. Cell 125(2):247-60. [PubMed: 16630814] [MGI Ref ID J:115870]
Trousdale RK; Wolgemuth DJ. 2004. Bromodomain containing 2 (Brd2) is expressed in distinct patterns during ovarian folliculogenesis independent of FSH or GDF9 action. Mol Reprod Dev 68(3):261-8. [PubMed: 15112318] [MGI Ref ID J:109752]
Wreford NG; Rajendra Kumar T; Matzuk MM; de Kretser DM. 2001. Analysis of the testicular phenotype of the follicle-stimulating hormone beta-subunit knockout and the activin type II receptor knockout mice by stereological analysis. Endocrinology 142(7):2916-20. [PubMed: 11416011] [MGI Ref ID J:71932]
Zhou J; Kumar TR; Matzuk MM; Bondy C. 1997. Insulin-like growth factor I regulates gonadotropin responsiveness in the murine ovary. Mol Endocrinol 11(13):1924-33. [PubMed: 9415397] [MGI Ref ID J:105227]
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
| Pricing for USA, Canada and Mexico shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00 Cryopreserved Embryos Fee $1600.00
| Pricing for International shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00 Cryopreserved Embryos Fee $2080.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 100903 B6129PF2/J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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