Strain Name:

B6;129S1-Il1raptm1Roml/J

Stock Number:

003284

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Availability:

Cryopreserved - Ready for recovery

Other products are available, see Purchasing Information for Cryopreserved Embryos

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Mark A. Labow,   Novartis Corporation

Description
Interleukin-1 receptor accessory protein (Il1rap)-null mice are viable and fertile. They exhibit loss of Il-1 receptor mediated signal transduction. Treatment of these mutant mice with IL-1 Alpha or IL-1 Beta results in no detectable increase in IL-6 levels in serum and fails to induce increased expression of E-selectin mRNA.

Control Information

  Control
   101045 B6129SF2/J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Il1raptm1Roml/Il1raptm1Roml

        involves: 129S1/Sv
  • immune system phenotype
  • decreased interleukin-6 secretion
    • failure of tissues to secrete Il-6 in response to Il1 injections as occurs in control mice   (MGI Ref ID J:68065)
    • mice otherwise normal   (MGI Ref ID J:68065)
  • integument phenotype
  • *normal* integument phenotype
    • mice have normal skin   (MGI Ref ID J:126327)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Il1raptm1Roml related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Il1raptm1Roml
Allele Name targeted mutation 1, Mark A Labow
Allele Type Targeted (knock-out)
Common Name(s) IL-1RAcP-;
Mutation Made ByDr. Mark Labow,   Novartis Corporation
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameW9.5/W95
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Il1rap, interleukin 1 receptor accessory protein
Chromosome 16
Gene Common Name(s) 6430709H04Rik; AI255955; AV239853; C3orf13; IL-1R AcP; IL-1RAcP; IL1R3; Il1racpb; RIKEN cDNA 6430709H04 gene; expressed sequence AI255955; expressed sequence AV239853;
Molecular Note A neomycin resistance cassette replaced a genomic fragment containing exons D1 and part of D2A. These exons encode the first Ig domain and part of the second Ig domain. Northern blot analysis revealed that a truncated mRNA was produced from this allele, but equilibrium binding studies demonstrated that no protein was present on the cell surface of primary mouse embryo fibroblasts (PMEFs) derived from homozygous mice. [MGI Ref ID J:68065]

Genotyping

Genotyping Information

Genotyping Protocols

Il1raptm1Roml, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cullinan EB; Kwee L; Nunes P; Shuster DJ; Ju G; McIntyre KW; Chizzonite RA; Labow MA. 1998. IL-1 receptor accessory protein is an essential component of the IL-1 receptor. J Immunol 161(10):5614-20. [PubMed: 9820540]  [MGI Ref ID J:68065]

Additional References

Il1raptm1Roml related

Andre R; Moggs JG; Kimber I; Rothwell NJ; Pinteaux E. 2006. Gene regulation by IL-1beta independent of IL-1R1 in the mouse brain. Glia 53(5):477-83. [PubMed: 16358337]  [MGI Ref ID J:156138]

Blumberg H; Dinh H; Trueblood ES; Pretorius J; Kugler D; Weng N; Kanaly ST; Towne JE; Willis CR; Kuechle MK; Sims JE; Peschon JJ. 2007. Opposing activities of two novel members of the IL-1 ligand family regulate skin inflammation. J Exp Med 204(11):2603-14. [PubMed: 17908936]  [MGI Ref ID J:126327]

Chackerian AA; Oldham ER; Murphy EE; Schmitz J; Pflanz S; Kastelein RA. 2007. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J Immunol 179(4):2551-5. [PubMed: 17675517]  [MGI Ref ID J:151287]

Huang H; Chen HW; Evankovich J; Yan W; Rosborough BR; Nace GW; Ding Q; Loughran P; Beer-Stolz D; Billiar TR; Esmon CT; Tsung A. 2013. Histones activate the NLRP3 inflammasome in Kupffer cells during sterile inflammatory liver injury. J Immunol 191(5):2665-79. [PubMed: 23904166]  [MGI Ref ID J:205815]

Liang Y; Seymour RE; Sundberg JP. 2011. Inhibition of NF-kappaB signaling retards eosinophilic dermatitis in SHARPIN-deficient mice. J Invest Dermatol 131(1):141-9. [PubMed: 20811394]  [MGI Ref ID J:182100]

Rankin AL; Mumm JB; Murphy E; Turner S; Yu N; McClanahan TK; Bourne PA; Pierce RH; Kastelein R; Pflanz S. 2010. IL-33 induces IL-13-dependent cutaneous fibrosis. J Immunol 184(3):1526-35. [PubMed: 20042577]  [MGI Ref ID J:159528]

Smith DE; Lipsky BP; Russell C; Ketchem RR; Kirchner J; Hensley K; Huang Y; Friedman WJ; Boissonneault V; Plante MM; Rivest S; Sims JE. 2009. A central nervous system-restricted isoform of the interleukin-1 receptor accessory protein modulates neuronal responses to interleukin-1. Immunity 30(6):817-31. [PubMed: 19481478]  [MGI Ref ID J:150078]

Thomas HE; Irawaty W; Darwiche R; Brodnicki TC; Santamaria P; Allison J; Kay TW. 2004. IL-1 Receptor Deficiency Slows Progression to Diabetes in the NOD Mouse. Diabetes 53(1):113-121. [PubMed: 14693705]  [MGI Ref ID J:87251]

Wolf G; Yirmiya R; Goshen I; Iverfeldt K; Holmlund L; Takeda K; Shavit Y. 2003. Impairment of interleukin-1 (IL-1) signaling reduces basal pain sensitivity in mice: genetic, pharmacological and developmental aspects. Pain 104(3):471-80. [PubMed: 12927619]  [MGI Ref ID J:118738]

Yoshida T; Shiroshima T; Lee SJ; Yasumura M; Uemura T; Chen X; Iwakura Y; Mishina M. 2012. Interleukin-1 receptor accessory protein organizes neuronal synaptogenesis as a cell adhesion molecule. J Neurosci 32(8):2588-600. [PubMed: 22357843]  [MGI Ref ID J:182360]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101045 B6129SF2/J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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