Strain Name:

C57BL/6-Tg(LIPC)6784His/J

Stock Number:

003285

Availability:

Repository-Cryopreserved

Description

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
GenerationF?+2
 
Donating Investigator Joshua Schultz,   Tularik Pharmaceutical Co.

Description
These transgenic mice overproduce a range of human hepatic lipase activities (4-23 fold increase) and help to understand the role of hepatic lipase in lipoprotein metabolism. A 5-fold increase in heparin releasable hepatic lipase activity is accompanied by moderate decreases in plasma total and high density lipoprotein (HDL) cholesterol and phospholipid (PL) but no significant change in triglyceride (TG), whereas a 23-fold increase in hepatic lipase activity causes a more significant decrease in plasma total and HDL cholesterol, PL and TG, and a substantial decrease in lipoprotein lipids amongst IDL, LDL and HDL fractions. High levels of hepatic lipase activity diminish the plasma concentration of apoA-I, A-II and apoE. Increased hepatic lipase activity is associated with a progressive decrease in the levels and an increase in the density of LpAI and LpB48 particles. There is an increased rate of disappearance of 125I-labeled human HDL from the plasma of the transgenic mice and substantially decreased apoB100-containing low density lipoproteins (LDL) compared to controls.

Related Strains

View Strains carrying other alleles of APOE     (4 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Hypocholesterolemia
Hypotriglyceridemia

Metabolism Research
Lipid Metabolism

APOE related

Mouse/Human Gene Homologs
Alzheimer's

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(LIPC)6784His
Allele Name transgene insertion 6784, Joshua R Schultz
Allele Type Transgenic (random, expressed)
Mutation Made By Joshua Schultz,   Tularik Pharmaceutical Co.
Strain of OriginC57BL/6
Expressed Gene LIPC, lipase, hepatic, human
Promoter APOE, apolipoprotein E, human
Molecular Note The transgene contains human hepatic lipase (LIPC) controlled by the human APOE promoter. [MGI Ref ID J:48486]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(LIPC)6784His, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Braschi S; Couture N; Gambarotta A; Gauthier BR; Coffill CR; Sparks DL; Maeda N; Schultz JR. 1998. Hepatic lipase affects both HDL and ApoB-containing lipoprotein levels in the mouse. Biochim Biophys Acta 1392(2-3):276-90. [PubMed: 9630674]  [MGI Ref ID J:48486]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.

General Terms and Conditions


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Terms of Use


General Terms and Conditions


Contact information

General inquiries

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