Strain Name: |
C57BL/6-Tg(LIPC)6784His/J |
|---|---|
Stock Number: |
003285 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | APOE; LIPC; Tg(LIPC)6784His; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Joshua Schultz, Tularik Pharmaceutical Co. Generation F?+2 Strain Description
These transgenic mice overproduce a range of human hepatic lipase activities (4-23 fold increase) and help to understand the role of hepatic lipase in lipoprotein metabolism. A 5-fold increase in heparin releasable hepatic lipase activity is accompanied by moderate decreases in plasma total and high density lipoprotein (HDL) cholesterol and phospholipid (PL) but no significant change in triglyceride (TG), whereas a 23-fold increase in hepatic lipase activity causes a more significant decrease in plasma total and HDL cholesterol, PL and TG, and a substantial decrease in lipoprotein lipids amongst IDL, LDL and HDL fractions. High levels of hepatic lipase activity diminish the plasma concentration of apoA-I, A-II and apoE. Increased hepatic lipase activity is associated with a progressive decrease in the levels and an increase in the density of LpAI and LpB48 particles. There is an increased rate of disappearance of 125I-labeled human HDL from the plasma of the transgenic mice and substantially decreased apoB100-containing low density lipoproteins (LDL) compared to controls.
Gene & Allele Details
| Allele Symbol | Tg(LIPC)6784His | ||
|---|---|---|---|
| Allele Name | transgene insertion 6784, Joshua R Schultz | ||
| Mutation Made By | Joshua Schultz, Tularik Pharmaceutical Co. | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | LIPC, lipase, hepatic, human | ||
| Promoter | APOE, apolipoprotein E, human | ||
| Molecular Note | The transgene contains human hepatic lipase (LIPC) controlled by the human APOE promoter. [MGI Ref ID J:48486] | ||
Genotyping Protocols
Tg(LIPC)6784His
Related Strains
Strains carrying other alleles of APOE
004632 B6.Cg-Tg(GFAP-APOE*2)14Hol Apoetm1Unc/J 004631 B6.Cg-Tg(GFAP-APOE*4)1Hol Apoetm1Unc/J 008408 B6;D2-Tg(APOE-NPC1L1)20Lqyu/J 007744 C57BL/6-Tg(APOE-DGAT2)24Far/J View Strains carrying other alleles of APOE (4 strains)
Research Applications
This mouse can be used to support research in many areas including:
APOE relatedCardiovascular Research
Hypocholesterolemia
Hypotriglyceridemia
Metabolism Research
Lipid Metabolism
Mouse/Human Gene Homologs
Alzheimer's
References
Selected Reference(s)
Braschi S; Couture N; Gambarotta A; Gauthier BR; Coffill CR; Sparks DL; Maeda N; Schultz JR. 1998. Hepatic lipase affects both HDL and ApoB-containing lipoprotein levels in the mouse. Biochim Biophys Acta 1392(2-3):276-90. [PubMed: 9630674] [MGI Ref ID J:48486]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(LIPC)6784His/J |
| Stock Number: | 003285 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form