Strain Name: |
C57BL/6-Tg(CAG-EGFP)1Osb/J |
|---|---|
Stock Number: |
003291 |
Availability: | Level 4 |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
C57BL/6-Tg(ACTB-EGFP)1Osb/J (Changed: 08-MAY-08
) |
|
C57BL/6-Tg(ACTbEGFP)1Osb/J (Changed: 15-DEC-04
) | |
| Genes & Alleles | ACTB; GFP; Tg(CAG-EGFP)1Osb; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Inbred x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Masaru Okabe, Osaka University Generation N?+20 (20-DEC-06) Appearance
black
Related Genotype: a/aStrain Description
This transgenic mouse line with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer makes all of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. Note that mice homozygous for this transgene die within the first two weeks following birth.Strain Development
The EcoRI sites included in the PCR primers were used to introduce the amplified EGFP cDNA into a pCAGGS expression vector containing the chicken beta-actin promoter and cytomegalovirus enhancer, beta-actin intron and bovine globin poly-adenylation signal. The entire insert with the promoter and coding sequence was excised with Bam-HI and SalI and gel-purified. This strain was generated in C57BL/6 mice.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(CAG-EGFP)1Osb | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Research Institute for Microbial Diseases, Osaka University | ||
| Common Name(s) | Tg(ACTB-EGFP)1Osb; Tg(ACTbEGFP)1Osb; TgN(ACTbEGFP)1Osb; TgN(beta-act-EFGP)01Osb; | ||
| Mutation Made By | Masaru Okabe, Osaka University | ||
| Strain of Origin | B6C3F2 | ||
| Site of Expression | All tissues, with the exception of erythrocytes and hair, appear green under excitation light. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | cDNA encoding enhanced green fluorescent protein (EGFP) was adjoined to the chicken beta actin promoter and cytomegalovirus enhancer. A bovine globin polyadenylation signal was also included in the construct. [MGI Ref ID J:87415] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | This strain is maintained by mating a hemizygous carrier with a C57BL/6J mouse. Mice homozygous for this transgene are perinatal lethals and die within the first two weeks following birth. Expected coat color from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (139 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
JAX Notes, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX Notes, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Animal Health Reports
Room Number AX1
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cardiovascular Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Reproductive Biology Research
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesOkabe M; Ikawa M; Kominami K; Nakanishi T; Nishimune Y. 1997. 'Green mice' as a source of ubiquitous green cells. FEBS Lett 407(3):313-9. [PubMed: 9175875] [MGI Ref ID J:87415]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(CAG-EGFP)1Osb/J |
| Stock Number: | 003291 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| 3-5 weeks | $195.70 | Hemizygous for Tg(CAG-EGFP)1Osb | Female | ||
| 6 weeks | $199.60 | Hemizygous for Tg(CAG-EGFP)1Osb | Female | ||
| 7 weeks | $203.50 | Hemizygous for Tg(CAG-EGFP)1Osb | Female | ||
| 8 weeks | $207.40 | Hemizygous for Tg(CAG-EGFP)1Osb | Female | ||
| 9 weeks | $211.30 | Hemizygous for Tg(CAG-EGFP)1Osb | Female | ||
| 10 weeks | $215.20 | Hemizygous for Tg(CAG-EGFP)1Osb | Female | ||
| 11 weeks | $219.10 | Hemizygous for Tg(CAG-EGFP)1Osb | Female | ||
| 12 weeks | $223.00 | Hemizygous for Tg(CAG-EGFP)1Osb | Female | ||
| 3-5 weeks | $195.70 | Hemizygous for Tg(CAG-EGFP)1Osb | Male | ||
| 6 weeks | $199.60 | Hemizygous for Tg(CAG-EGFP)1Osb | Male | ||
| 7 weeks | $203.50 | Hemizygous for Tg(CAG-EGFP)1Osb | Male | ||
| 8 weeks | $207.40 | Hemizygous for Tg(CAG-EGFP)1Osb | Male | ||
| 9 weeks | $211.30 | Hemizygous for Tg(CAG-EGFP)1Osb | Male | ||
| 10 weeks | $215.20 | Hemizygous for Tg(CAG-EGFP)1Osb | Male | ||
| 11 weeks | $219.10 | Hemizygous for Tg(CAG-EGFP)1Osb | Male | ||
| 12 weeks | $223.00 | Hemizygous for Tg(CAG-EGFP)1Osb | Male | ||
| Pair | $216.50 | C57BL/6J (000664) x Hemizygous for Tg(CAG-EGFP)1Osb | |||
| Pair | $215.10 | Hemizygous for Tg(CAG-EGFP)1Osb x C57BL/6J (000664) | |||
Supply Details
| Standard Supply | Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one-three months. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available. Strains that must be genotyped are not available until five to seven weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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