Strain Name: |
129S-Gt(ROSA)26Sortm1Sor/J |
|---|---|
Stock Number: |
003310 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Gt(ROSA)26Sor; Gt(ROSA)26Sortm1Sor; lacZ; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Generation N?+5p Appearance
white-bellied agouti
Related Genotype: Aw/AwStrain Description
Mice heterozygous or homozygous for the Gtrosa26tm1Sor targeted mutation may be used to test the tissue/cellular expression pattern of the cre transgene in any transgenic strain carrying cre under the regulation of a specific promoter. Cre expression results in the removal of a loxP-flanked DNA segment that prevents expression of a lacZ gene. When crossed with a cre transgenic strain, lacZ is expressed in cells/tissues where cre is expressed. The 129-Gtrosa26tm1Sor strain is particularly useful for this purpose because the ROSA26 promoter leads to generalized expression of lacZ during development or in the adult.Strain Development
The ROSA26 locus of a gene trap strain was targeted with a ROSA26 reporter (R26R) construct to generate a Cre reporter strain by homologous recombination in 129S4/SvJaeSor-derived AK7 ES cells. The R26R construct was created by subcloning the pROSA26-1 vector, and inserting a splice acceptor sequence (identical to the one used in the original gene trap allele), a neo expression cassette flanked by loxP sites, a lacZ gene and a polyadenylation sequence at a unique site approximately 300-bp 5' of the original gene trap integration site. Transcriptional read-through was prevented with the use of a triple polyadenylation sequence at the 3' end of the neo expression cassette. Upon arrival at The Jackson Laboratory, mutant mice were crossed to Stock No. 002448 (129S1/SvImJ) for more than 6 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Gt(ROSA)26Sortm1Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Philippe Soriano | ||
| Common Name(s) | Gt(ROSA)26Sor; Gtrosa26tm/Sor; Gtrosa26tm1Sor; R26LacZ; R26R; R26R-lacZ; R26RLacZ; ROSA26-LacZ; ROSA26-loxP; ROSA26fl; ROSA26R; Rosa26 cre reporter; Rosa26-LoxSTOPLox-LacZ; Rosa26-lacZ cre reporter; Rosa26stop-LacZ; Rosa26R-lacZ; Rosa26RLacZ; Rosa26RlacZ; Rosa26r-; RosaLoxP; loxP-Stop-loxP lacZ reporter; | ||
| Mutation Made By | Philippe Soriano, Fred Hutchinson Cancer Research Center | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | when crossed to a Cre recombinase-expressing strain, lacZ expression is observed in the cre-expressing tissues | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A targeting vector was designed from the original gene trap strain, ROSA beta-geo26, to include a splice acceptor sequence (SA), a neo expression cassette flanked by loxP sites, a lacZ gene, and a polyadenylation (bpA) sequence inserted at a unique Xba1 site approximately 300-bp 5' of the original gene-trap integration site. In addition, a triple polyadenylation sequence was added to the 3' end of the neo expression cassette to prevent transcriptional read-through. Presence of the floxed neo cassette prevents lacZ expression. When crossed with a Cre transgenic strain, lacZ is expressed in all cells/tissue where Cre is expressed. [MGI Ref ID J:64292] [MGI Ref ID J:92442] | ||
Control Information
| Allele | Control | |
|---|---|---|
| Gt(ROSA)26Sortm1Sor | Wild-type from the colony | |
| Gt(ROSA)26Sortm1Sor | 002448 129S1/SvImJ | (approximate) |
| Wildtype mice from the colony should be used as controls. 129S3/SvImJ mice (Stock No. 002448) may serve as an approximate control as the Gtrosa26tm1Sor targeted mutation is currently being backcrossed to this strain (currently N?+1). The background of the original imported strain was 129S4/SvJaeSor which is a match to the AK7 ES cell line used. | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gt(ROSA)26Sortm1Sor
Colony Maintenance
| Breeding & Husbandry | This maintaining a live colony, mutant mice are bred to JR# 002448 (129S1/SvImJ). Expected coat color from breeding:White Bellied Agouti |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
003474 B6.129S4-Gt(ROSA)26Sortm1Sor/J 003309 B6;129S4-Gt(ROSA)26Sortm1Sor/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
New 129 Nomenclature Bulletin
Research Applications
This mouse can be used to support research in many areas including:
lacZ relatedResearch Tools
lacZ Expression
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System: ubiquitously expressed lox-STOP-lox-LacZ transgene)
Genetics Research (Tissue/Cell Markers: Cre-lox System: ubiquitously expressed lox-STOP-lox-LacZ transgene)
Research Tools
lacZ Expression
References
Selected Reference(s)
Additional ReferencesSoriano P. 1999. Generalized lacZ expression with the ROSA26 Cre reporter strain [letter] Nat Genet 21(1):70-1. [PubMed: 9916792] [MGI Ref ID J:64292]
Price and Supply Information
| Strain Name: | 129S-Gt(ROSA)26Sortm1Sor/J |
| Stock Number: | 003310 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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