Strain Name: |
FVB/N-Tg(EIIa-cre)C5379Lmgd/J |
|---|---|
Stock Number: |
003314 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
FVB/N-TgN(EIIa-Cre)C5379Lmgd (Changed: 15-DEC-04
) |
| Genes & Alleles | cre; Ella; Tg(EIIa-cre)C5379Lmgd; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Heiner Westphal, National Institutes of Health Generation F?+F21 (08-JAN-08) Strain Description
This line carries a Cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre expression is thought to occur prior to implantation in the uterine wall. A mosaic pattern of expression is commonly observed. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny.Strain Development
The EIIa-Cre plasmid was constructed by replacing the human cytomegalovirus promoter in pBS185 with the adenovirus EIIa promoter from pEII-lacZ. cre activity present in zygotes of the EIIa-cre genotype was measured as follows. EIIa-cre/EIIa-cre homozygous animals were mated with +/alphaA-loxP-STOP-loxP-TAg heterozygous animals and the presence of lens tumors in double transgenic progeny, indicative of cre-mediated excision of STOP sequences and a concomitant activation of SV40 TAg, was scored. The strain originated on an FVB/N background. The strain is homozygous for the transgene.
Gene & Allele Details
| Allele Symbol | Tg(EIIa-cre)C5379Lmgd | ||
|---|---|---|---|
| Allele Name | transgene insertion C5379, Laboratory of Mammalian Genes and Development, Heiner Westphal | ||
| Common Name(s) |
E2a-Cre;
EII-cre;
EIIa Cre | ||
| Mutation Made By | Heiner Westphal, National Institutes of Health | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | widespread expression (including germline); mosaic pattern commonly observed | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ella, adenovirus, adenovirus | ||
| Molecular Note | The adenovirus EIIa promoter directs expression of Cre recombinase in preimplantation mouse embryos. This transgene expresses Cre recombinase in nearly all tissues. [MGI Ref ID J:137185] [MGI Ref ID J:70555] [MGI Ref ID J:97109] | ||
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Generic Cre
Generic Cre QPCR
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying Tg(EIIa-cre)C5379Lmgd allele
003724 B6.FVB-Tg(EIIa-cre)C5379Lmgd/J View Strains carrying Tg(EIIa-cre)C5379Lmgd (1 strain)
Additional Web Information
Cre-lox or FLP-FRT Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesLakso M; Pichel JG; Gorman JR; Sauer B; Okamoto Y; Lee E; Alt FW; Westphal H. 1996. Efficient in vivo manipulation of mouse genomic sequences at the zygote stage. Proc Natl Acad Sci U S A 93(12):5860-5. [PubMed: 8650183] [MGI Ref ID J:70555]
Price and Supply Information
| Strain Name: | FVB/N-Tg(EIIa-cre)C5379Lmgd/J |
| Stock Number: | 003314 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $110.40 | Homozygous for Tg(EIIa-cre)C5379Lmgd | |||
| Pair | $220.80 | Homozygous for Tg(EIIa-cre)C5379Lmgd x Homozygous for Tg(EIIa-cre)C5379Lmgd | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
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Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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