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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names FVB/N-TgN(TetORo1-lacZ)3Conk (Changed: 15-DEC-04 ) FVB/N-TgN(TetORo1LacZ)3Conk (Changed: 15-DEC-04 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Bruce Conklin, Gladstone Institutes Appearance
albino
Related Genotype: Tyrc/TyrcDescription
Transgenic mice express both Ro1 (Receptor Activated Solely by a Synthetic Ligand (RASSL), opioid, #1) and lacZ under the regulation of tetracycline responsive elements (TRE; tetO)(see Strain Development for more information). When Ro1 is expressed in the heart (by crossing it with strain FVB.Cg-Tg(Myh6-tTA)6Smbf/J - Stock No. 003170), administration of the drug spiradoline produces a dramatic decrease in heart rate. Since Ro1 and lacZ are co-integrated and under tetracycline/doxycycline control, X-gal staining can be used to identify cells in which the tet-inducible system is working. Ro1 activates Gi-signaling in response to spiradoline, and can be used to study the effect of Gi-signaling in many tissues. In the heart, Ro1-mediated activation of Gi-signaling slows heart rate, but in other tissues it is predicted to control diverse physiological events, such as cell proliferation, secretion, and neurotransmission.Development
Transgenic mice contain two co-integrated transgenes: tetO-Ro1 (Receptor Activated Solely by a Synthetic Ligand (RASSL) opioid, #1) and tetO-lacZ. Both target genes, Ro1 and lacZ, are under the control of tetracycline responsive elements (TRE; tetO). Ro1 is a prototype RASSL that is derived from a human Kappa Opioid Receptor (G protein-coupled receptor) that has been altered to reduce the activation by endogenous hormones but preserves activation by an exogenous drug, spiradoline, allowing experimental control of G protein signaling. An N-terminal FLAG epitope that is extra-cellularly expressed and that can be detected with the FLAG monoclonal antibody (Kodak) was also incorporated into the transgenic Ro1 protein. The tetO-lacZ and tetO-Ro1 transgenes were coinjected into FVB/N fertilized embryos. The two transgenes are co-integrated such that beta-galactosidase can be used as a marker for tetracycline-induced expression.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (245 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
lacZ Expression
Cardiovascular Research
Tetop Tet System
Neurobiology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(tetORo1-lacZ)3Conk | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, Bruce Conklin | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | TRE-lacZ; | ||
| Mutation Made By | Bruce Conklin, Gladstone Institutes | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Inducible, conditional | ||
| Expressed Gene | OPRK1, opioid receptor, kappa 1, human | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | tetO, tet operator, | ||
| General Note | On an FVB/N genetic background, transgenic mice also carrying Tg(Myh6-tTA)6Smbf express Ro1 in the heart. Administration of the drug spiradoline results in a dramatic decrease of heart rate in these mice. X-gal staining of tissues from these mice revealsthe pattern of cells that the tet system is working in. Ro1 activates Gi-signaling in response to spiradoline. | ||
| Molecular Note | Two transgenes controlled by the tetO (responsive to tet-transactivator) promoter, one containing "Ro1" and the other containing lacZ, were co-integrated so that beta-galactosidase could be used as a marker for expression. Ro1 is a prototype RASSL (Receptor Activated Solely by a Synthetic Ligand) with an N-terminal FLAG epitope that is extra-cellularly expressed and can be detected with the FLAG monoclonal antibody. Ro1 (RASSL, opioid, #1) is derived from a Kappa Opioid Receptor (G protein-coupled receptor) that was altered to reduce the activation by endogenous hormones, but preserves activation by an exogenous drug, spiradoline, allowing experimental control of G protein signaling. [MGI Ref ID J:95090] | ||
| Gene Symbol and Name | Tg(tetORo1-lacZ)3Conk, transgene insertion 3, Bruce Conklin | ||
| Chromosome | UN | ||
| Gene Common Name(s) | TRE-lacZ; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Redfern CH; Coward P; Degtyarev MY; Lee EK; Kwa AT; Hennighausen L; Bujard H; Fishman GI; Conklin BR. 1999. Conditional expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice. Nat Biotechnol 17(2):165-9. [PubMed: 10052353] [MGI Ref ID J:95090]
Additional References
Tg(tetORo1-lacZ)3Conk relatedBaker AJ; Redfern CH; Harwood MD; Simpson PC; Conklin BR. 2001. Abnormal contraction caused by expression of G(i)-coupled receptor in transgenic model of dilated cardiomyopathy. Am J Physiol Heart Circ Physiol 280(4):H1653-9. [PubMed: 11247776] [MGI Ref ID J:128598]
Carpenter B; Lin Y; Stoll S; Raffai RL; McCuskey R; Wang R. 2005. VEGF is crucial for the hepatic vascular development required for lipoprotein uptake. Development 132(14):3293-303. [PubMed: 15944181] [MGI Ref ID J:100427]
Chang MA; Horner JW; Conklin BR; DePinho RA; Bok D; Zack DJ. 2000. Tetracycline-inducible system for photoreceptor-specific gene expression. Invest Ophthalmol Vis Sci 41(13):4281-7. [PubMed: 11095627] [MGI Ref ID J:134762]
Chikama T; Hayashi Y; Liu CY; Terai N; Terai K; Kao CW; Wang L; Hayashi M; Nishida T; Sanford P; Doestchman T; Kao WW. 2005. Characterization of tetracycline-inducible bitransgenic Krt12rtTA/+/tet-O-LacZ mice. Invest Ophthalmol Vis Sci 46(6):1966-72. [PubMed: 15914610] [MGI Ref ID J:99414]
Dupays L; Kotecha S; Angst B; Mohun TJ. 2009. Tbx2 misexpression impairs deployment of second heart field derived progenitor cells to the arterial pole of the embryonic heart. Dev Biol 333(1):121-31. [PubMed: 19563797] [MGI Ref ID J:152254]
McCloskey DT; Turcato S; Wang GY; Turnbull L; Zhu BQ; Bambino T; Nguyen AP; Lovett DH; Nissenson RA; Karliner JS; Baker AJ. 2008. Expression of a Gi-coupled receptor in the heart causes impaired Ca2+ handling, myofilament injury, and dilated cardiomyopathy. Am J Physiol Heart Circ Physiol 294(1):H205-12. [PubMed: 17965283] [MGI Ref ID J:132451]
Peng J; Bencsik M; Louie A; Lu W; Millard S; Nguyen P; Burghardt A; Majumdar S; Wronski TJ; Halloran B; Conklin BR; Nissenson RA. 2008. Conditional expression of a gi-coupled receptor in osteoblasts results in trabecular osteopenia. Endocrinology 149(3):1329-37. [PubMed: 18048501] [MGI Ref ID J:131737]
Redfern CH; Degtyarev MY; Kwa AT; Salomonis N; Cotte N; Nanevicz T; Fidelman N; Desai K; Vranizan K; Lee EK; Coward P; Shah N; Warrington JA; Fishman GI; Bernstein D; Baker AJ; Conklin BR. 2000. Conditional expression of a Gi-coupled receptor causes ventricular conduction delay and a lethal cardiomyopathy. Proc Natl Acad Sci U S A 97(9):4826-31. [PubMed: 10781088] [MGI Ref ID J:107363]
Shigehara T; Zaragoza C; Kitiyakara C; Takahashi H; Lu H; Moeller M; Holzman LB; Kopp JB. 2003. Inducible podocyte-specific gene expression in transgenic mice. J Am Soc Nephrol 14(8):1998-2003. [PubMed: 12874453] [MGI Ref ID J:109826]
Sweger EJ; Casper KB; Scearce-Levie K; Conklin BR; McCarthy KD. 2007. Development of hydrocephalus in mice expressing the G(i)-coupled GPCR Ro1 RASSL receptor in astrocytes. J Neurosci 27(9):2309-17. [PubMed: 17329428] [MGI Ref ID J:119818]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
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| phone: | 207-288-6470 |
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