Strain Name: |
FVB/N-Tg(tetORo1-lacZ)3Conk/J |
|---|---|
Stock Number: |
003315 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
FVB/N-TgN(TetORo1-lacZ)3Conk (Changed: 15-DEC-04
) |
|
FVB/N-TgN(TetORo1LacZ)3Conk (Changed: 15-DEC-04
) | |
| Genes & Alleles | OPRK1; lacZ; Tg(tetORo1-lacZ)3Conk; tetO; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Bruce Conklin, Gladstone Institutes Generation N?+5pN1 Appearance
albino
Related Genotype: Tyrc/TyrcStrain Description
Transgenic mice express both Ro1 (Receptor Activated Solely by a Synthetic Ligand (RASSL), opioid, #1) and lacZ under the regulation of tetracycline responsive elements (TRE; tetO)(see Strain Development for more information). When Ro1 is expressed in the heart (by crossing it with strain FVB.Cg-Tg(Myh6-tTA)6Smbf/J - Stock No. 003170), administration of the drug spiradoline produces a dramatic decrease in heart rate. Since Ro1 and lacZ are co-integrated and under tetracycline/doxycycline control, X-gal staining can be used to identify cells in which the tet-inducible system is working. Ro1 activates Gi-signaling in response to spiradoline, and can be used to study the effect of Gi-signaling in many tissues. In the heart, Ro1-mediated activation of Gi-signaling slows heart rate, but in other tissues it is predicted to control diverse physiological events, such as cell proliferation, secretion, and neurotransmission.Strain Development
Transgenic mice contain two co-integrated transgenes: tetO-Ro1 (Receptor Activated Solely by a Synthetic Ligand (RASSL) opioid, #1) and tetO-lacZ. Both target genes, Ro1 and lacZ, are under the control of tetracycline responsive elements (TRE; tetO). Ro1 is a prototype RASSL that is derived from a human Kappa Opioid Receptor (G protein-coupled receptor) that has been altered to reduce the activation by endogenous hormones but preserves activation by an exogenous drug, spiradoline, allowing experimental control of G protein signaling. An N-terminal FLAG epitope that is extra-cellularly expressed and that can be detected with the FLAG monoclonal antibody (Kodak) was also incorporated into the transgenic Ro1 protein. The tetO-lacZ and tetO-Ro1 transgenes were coinjected into FVB/N fertilized embryos. The two transgenes are co-integrated such that beta-galactosidase can be used as a marker for tetracycline-induced expression.
Gene & Allele Details
| Allele Symbol | Tg(tetORo1-lacZ)3Conk | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, Bruce Conklin | ||
| Mutation Made By | Bruce Conklin, Gladstone Institutes | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Inducible, conditional | ||
| Expressed Gene | OPRK1, opioid receptor, kappa 1, human | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | tetO, tet operator, | ||
| General Note | On an FVB/N genetic background, transgenic mice also carrying Tg(Myh6-tTA)6Smbf express Ro1 in the heart. Administration of the drug spiradoline results in a dramatic decrease of heart rate in these mice. X-gal staining of tissues from these mice revealsthe pattern of cells that the tet system is working in. Ro1 activates Gi-signaling in response to spiradoline. | ||
| Molecular Note | Two transgenes controlled by the tetO (responsive to tet-transactivator) promoter, one containing "Ro1" and the other containing lacZ, were co-integrated so that beta-galactosidase could be used as a marker for expression. Ro1 is a prototype RASSL (Receptor Activated Solely by a Synthetic Ligand) with an N-terminal FLAG epitope that is extra-cellularly expressed and can be detected with the FLAG monoclonal antibody. Ro1 (RASSL, opioid, #1) is derived from a Kappa Opioid Receptor (G protein-coupled receptor) that was altered to reduce the activation by endogenous hormones, but preserves activation by an exogenous drug, spiradoline, allowing experimental control of G protein signaling. [MGI Ref ID J:95090] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(LacZ) Generic
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Research Applications
This mouse can be used to support research in many areas including:
lacZ relatedResearch Tools
lacZ Expression
Cardiovascular Research (Tetop Tet System)
Neurobiology Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Responsive Strains)
Research Tools
lacZ Expression
References
Selected Reference(s)
Additional ReferencesRedfern CH; Coward P; Degtyarev MY; Lee EK; Kwa AT; Hennighausen L; Bujard H; Fishman GI; Conklin BR. 1999. Conditional expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice. Nat Biotechnol 17(2):165-9. [PubMed: 10052353] [MGI Ref ID J:95090]
Price and Supply Information
| Strain Name: | FVB/N-Tg(tetORo1-lacZ)3Conk/J |
| Stock Number: | 003315 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
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- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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