Strain Name: |
129-Tg(Prm-cre)58Og/J |
|---|---|
Stock Number: |
003328 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Prm1; cre; Tg(Prm-cre)58Og; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Stephen O'Gorman, Case Western Reserve University Generation N?F?+N2F19 (31-DEC-07) Strain Description
Mice homozygous for this PrmCre transgene are viable and fertile. Embryonic stem cells containing recombinase transgenes that are expressed in the male germ line, but not in other tissues or in the embryonic stem cells themselves, substantially simplify the production of subtle or conditional mutations in mice. This strain shows that transgenes comprised of the mouse protamine 1 promoter and the Cre recombinase coding sequence mediate the efficient recombination of a cre target transgene in the male germ line, but not in other tissues. This system can be used for reducing the time, effort, and resources required to produce homologously recombined alleles in mice that have been secondarily rearranged by site-specific recombinase.Strain Development
The PrmCre transgene was designed with the mouse protamine 1 promoter upstream of a modified Cre recombinase coding sequence (modified to contain a consensus translation start site, 11 codons for a human c-myc epitope, 7 codons for a minimal simian virus 40 nuclear localization signal, and a polyadenylation signal). This transgene was microinjected into pronuclei of fertilized 129/SvJae oocytes, and the resulting transgenic offspring were bred to 129/SvJae mice. Founder line 58 mice (with a single copy of the transgene) were backcrossed to 129Sv/Jae for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with 129S1/SvImJ (Stock No. 002448) for approximately two generations to establish the colony.
Gene & Allele Details
| Allele Symbol | Tg(Prm-cre)58Og | ||
|---|---|---|---|
| Allele Name | transgene insertion 58, Stephen O'Gorman | ||
| Common Name(s) | PrmCre; | ||
| Strain of Origin | 129S4/SvJae | ||
| Site of Expression | male germ line | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Prm1, protamine 1, mouse, laboratory | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of the mouse protamine (Prm1) promoter. The transgene directs expression of Cre recombinase in the male germ cells and not in other tissues. [MGI Ref ID J:117978] [MGI Ref ID J:67932] [MGI Ref ID J:98828] | ||
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred. Expected coat color from breeding is White Bellied Agouti. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Tg(Prm-cre)58Og allele
007252 B6Ei.129S4-Tg(Prm-cre)58Og/EiJ View Strains carrying Tg(Prm-cre)58Og (1 strain)
Additional Web Information
Cre-lox or FLP-FRT Systems
JAX Notes, Summer 2001; 482. Cre Transgenic Strains for Conditional Mutagenesis.
New 129 Nomenclature Bulletin
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesO'Gorman S; Dagenais NA; Qian M; Marchuk Y. 1997. Protamine-Cre recombinase transgenes efficiently recombine target sequences in the male germ line of mice, but not in embryonic stem cells. Proc Natl Acad Sci U S A 94(26):14602-7. [PubMed: 9405659] [MGI Ref ID J:67932]
O'Gorman S; Sidman RL. 1985. Degeneration of thalamic neurons in Purkinje cell degeneration mutant mice. I. Distribution of neuron loss. J Comp Neurol 234(3):277-97. [PubMed: 3988985] [MGI Ref ID J:7819]
Price and Supply Information
| Strain Name: | 129-Tg(Prm-cre)58Og/J |
| Stock Number: | 003328 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form