Strain Name:

B6.129S7-Itgb2tm2Bay/J

Stock Number:

003329

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S2 via AB1 ES cell
 
Donating InvestigatorDr. Arthur Beaudet,   Baylor College of Medicine

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Itgb2tm2Bay targeted mutation are viable and fertile, though not good breeders. This mutation differs from the Itgb2tm1Bay mutation (Stock No. 2128) in that it is a null mutation for the Itgb2 subunit of leukocyte integrins. Mutant mice develop chronic dermatitis with extensive facial and submandibular erosions. They also exhibit elevated neutrophil counts, increased immunoglobulin levels, lymphadenopathy, splenomegaly, and abundant plasma cells in skin, lymph nodes, gut and kidney. A severe defect in T cell proliferation can be found in mutant mice when T cell receptors are stimulated either by staphylococcal enterotoxin A or by major histocompatibility complex alloantigens. The phenotype of the mutant mice resembles the phenotype for the human disorder Leukocyte Adhesion Deficiency Type I (LADI).

Control Information

  Control
   See control note: Wildtype mice from the colony or C57BL/6J mice (Stock No. 000664) may be used as controls.
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Itgb2tm2Bay allele
016897   NOD.129S7(B6)-Itgb2tm2Bay/CgkJ
View Strains carrying   Itgb2tm2Bay     (1 strain)

Strains carrying other alleles of Itgb2
018648   B6.129S6(Cg)-Itgb2tm1.1Mskim/J
002128   B6.129S7-Itgb2tm1Bay/J
View Strains carrying other alleles of Itgb2     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Leukocyte Adhesion Deficiency, Type I; LAD
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Itgb2tm2Bay/Itgb2tm2Bay

        involves: 129S7/SvEvBrd * C57BL/6J
  • mortality/aging
  • partial perinatal lethality
    • 10 - 40% of newborns died perinatally   (MGI Ref ID J:48542)
  • immune system phenotype
  • abnormal immune system cell morphology   (MGI Ref ID J:48542)
    • abnormal T cell proliferation
      • impaired T cell receptor (TCR)-dependent T cell proliferation   (MGI Ref ID J:48542)
    • abnormal neutrophil morphology
      • neutrophils showed hypersegmentation and appendix formation of the nuclei   (MGI Ref ID J:48542)
      • increased neutrophil cell number
        • increased leukocyte counts were largely due to elevated numbers of circulating neutrophils   (MGI Ref ID J:48542)
    • increased leukocyte cell number
      • numbers of circulating leukocytes were increased up to six-fold   (MGI Ref ID J:48542)
      • increased lymphocyte cell number   (MGI Ref ID J:48542)
      • increased monocyte cell number   (MGI Ref ID J:48542)
      • increased neutrophil cell number
        • increased leukocyte counts were largely due to elevated numbers of circulating neutrophils   (MGI Ref ID J:48542)
    • myeloid hyperplasia   (MGI Ref ID J:48542)
  • abnormal leukocyte physiology   (MGI Ref ID J:48542)
    • abnormal T cell proliferation
      • impaired T cell receptor (TCR)-dependent T cell proliferation   (MGI Ref ID J:48542)
    • abnormal neutrophil physiology
      • impaired neutrophil extravasation into the interstitial tissue   (MGI Ref ID J:48542)
      • stimulated neutrophils did not adhere to KLH-coated glass and observed no increase in cell adhesion and no decrease in the number of rolling cells upon fMLP stimulation   (MGI Ref ID J:48542)
    • increased immunoglobulin level   (MGI Ref ID J:48542)
      • increased IgA level
        • modestly increased at 2.5-3 months of age   (MGI Ref ID J:48542)
      • increased IgG level
        • serum total IgG, IgG1, IgG2a, and IgG2b concentrations were increased 10-15-fold at 2.5-3 months of age and IgG3 was modestly increased   (MGI Ref ID J:48542)
      • increased IgM level
        • modestly increased at 2.5-3 months of age   (MGI Ref ID J:48542)
  • abnormal lymph organ size   (MGI Ref ID J:48542)
    • enlarged lymph nodes
      • reactive lymphadenopathy with an accumulation of plasma cells   (MGI Ref ID J:48542)
    • enlarged spleen
      • due to a myeloid hyperplasia within the red pulp with weight increased by 2.5-fold   (MGI Ref ID J:48542)
  • increased circulating interleukin-3 level
    • serum level of IL-3 was increased by more than 20-fold   (MGI Ref ID J:48542)
  • increased circulating interleukin-6 level
    • serum level of IL-6 was increased by more than 20-fold   (MGI Ref ID J:48542)
  • increased inflammatory response
    • occasional inflammatory lesions were observed in the heart, salivary gland, liver, and intestine   (MGI Ref ID J:48542)
    • conjunctivitis   (MGI Ref ID J:48542)
    • dermatitis
      • surviving mice developed extended facial and submandibular ulcerative dermatitis at about 3 months of age, with the dermal inflammatory cell infiltrate consisting of eosinophils, lymphocytes, histiocytes, and occasional perivascular plasma cells, and fewer neutrophils than expected   (MGI Ref ID J:48542)
  • increased susceptibility to bacterial infection
    • increased susceptibility to death from S.pneumoniae infection, with no survival by day 10 of infection   (MGI Ref ID J:48542)
  • vision/eye phenotype
  • conjunctivitis   (MGI Ref ID J:48542)
  • hematopoietic system phenotype
  • abnormal leukocyte physiology   (MGI Ref ID J:48542)
    • abnormal T cell proliferation
      • impaired T cell receptor (TCR)-dependent T cell proliferation   (MGI Ref ID J:48542)
    • abnormal neutrophil physiology
      • impaired neutrophil extravasation into the interstitial tissue   (MGI Ref ID J:48542)
      • stimulated neutrophils did not adhere to KLH-coated glass and observed no increase in cell adhesion and no decrease in the number of rolling cells upon fMLP stimulation   (MGI Ref ID J:48542)
    • increased immunoglobulin level   (MGI Ref ID J:48542)
      • increased IgA level
        • modestly increased at 2.5-3 months of age   (MGI Ref ID J:48542)
      • increased IgG level
        • serum total IgG, IgG1, IgG2a, and IgG2b concentrations were increased 10-15-fold at 2.5-3 months of age and IgG3 was modestly increased   (MGI Ref ID J:48542)
      • increased IgM level
        • modestly increased at 2.5-3 months of age   (MGI Ref ID J:48542)
  • abnormal neutrophil morphology
    • neutrophils showed hypersegmentation and appendix formation of the nuclei   (MGI Ref ID J:48542)
    • increased neutrophil cell number
      • increased leukocyte counts were largely due to elevated numbers of circulating neutrophils   (MGI Ref ID J:48542)
  • enlarged spleen
    • due to a myeloid hyperplasia within the red pulp with weight increased by 2.5-fold   (MGI Ref ID J:48542)
  • increased bone marrow cell number   (MGI Ref ID J:48542)
  • increased leukocyte cell number
    • numbers of circulating leukocytes were increased up to six-fold   (MGI Ref ID J:48542)
    • increased lymphocyte cell number   (MGI Ref ID J:48542)
    • increased monocyte cell number   (MGI Ref ID J:48542)
    • increased neutrophil cell number
      • increased leukocyte counts were largely due to elevated numbers of circulating neutrophils   (MGI Ref ID J:48542)
  • myeloid hyperplasia   (MGI Ref ID J:48542)
  • homeostasis/metabolism phenotype
  • increased circulating interleukin-3 level
    • serum level of IL-3 was increased by more than 20-fold   (MGI Ref ID J:48542)
  • increased circulating interleukin-6 level
    • serum level of IL-6 was increased by more than 20-fold   (MGI Ref ID J:48542)
  • integument phenotype
  • alopecia
    • surviving mice developed facial alopecia   (MGI Ref ID J:48542)
  • dermatitis
    • surviving mice developed extended facial and submandibular ulcerative dermatitis at about 3 months of age, with the dermal inflammatory cell infiltrate consisting of eosinophils, lymphocytes, histiocytes, and occasional perivascular plasma cells, and fewer neutrophils than expected   (MGI Ref ID J:48542)
  • epidermal hyperplasia   (MGI Ref ID J:48542)
  • reddish skin
    • surviving mice developed reddening of the skin   (MGI Ref ID J:48542)

Itgb2tm2Bay/Itgb2tm2Bay

        involves: 129S7/SvEvBrd
  • immune system phenotype
  • decreased T cell proliferation
    • reduced staphylococcal enterotoxin A (SEA)-induced T cell proliferation in splenocytes compared to wild-type and was even more decreased than in homozygous Itgal mutant mice   (MGI Ref ID J:90954)
  • hematopoietic system phenotype
  • decreased T cell proliferation
    • reduced staphylococcal enterotoxin A (SEA)-induced T cell proliferation in splenocytes compared to wild-type and was even more decreased than in homozygous Itgal mutant mice   (MGI Ref ID J:90954)

Itgb2tm2Bay/Itgb2tm2Bay

        involves: 129S7/SvEvBrd * PL/J
  • integument phenotype
  • *normal* integument phenotype
    • do not develop chronic inflammatory skin disease   (MGI Ref ID J:84126)

Itgb2tm2Bay/Itgb2tm2Bay

        involves: 129S7/SvEvBrd * C57BL/6
  • immune system phenotype
  • decreased acute inflammation
    • in response to croton oil application to the ear in young mice (7-14 weeks), inflammation is reduced   (MGI Ref ID J:112286)
  • impaired neutrophil recruitment
    • defect in acute neutrophil emigration in older mice is observed   (MGI Ref ID J:112286)
  • increased neutrophil cell number
    • circulating neutrophils are increased in mutants and this increases with age of mice   (MGI Ref ID J:112286)
  • hematopoietic system phenotype
  • impaired neutrophil recruitment
    • defect in acute neutrophil emigration in older mice is observed   (MGI Ref ID J:112286)
  • increased neutrophil cell number
    • circulating neutrophils are increased in mutants and this increases with age of mice   (MGI Ref ID J:112286)
  • integument phenotype
  • spontaneous skin ulceration
    • mice develop spontaneous skin lesions characterized by mononuclear leukocytes   (MGI Ref ID J:112286)

Itgb2tm2Bay/Itgb2tm2Bay

        PL.129S7-Itgb2tm2Bay
  • immune system phenotype
  • decreased susceptibility to type IV hypersensitivity reaction
    • oxazolone treatment does not result in induction of allergic contact dermatitis as in wild-type mice and only a slight edema without inflammatory cells is seen or increase in T cells   (MGI Ref ID J:109598)
  • integument phenotype
  • *normal* integument phenotype
    • mice do not develop psoriasiform dermatitis during a 2 year observation period   (MGI Ref ID J:109598)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Itgb2tm2Bay related

Cell Biology Research
Defects in Cell Adhesion Molecules

Dermatology Research
Skin and Hair Texture Defects

Developmental Biology Research
Defects in Cell Adhesion Molecules
Defects in Extracellular Matrix Molecules

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Itgb2tm2Bay
Allele Name targeted mutation 2, Baylor College of Medicine
Allele Type Targeted (knock-out)
Common Name(s) CD18 null; CD18-; Itgb2-; beta2-;
Mutation Made ByDr. Arthur Beaudet,   Baylor College of Medicine
Strain of Origin129S7/SvEvBrd-Hprt<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name Itgb2, integrin beta 2
Chromosome 10
Gene Common Name(s) 2E6; AI528527; CD18; CD18 antigen; Cd18; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7; Mac-1 beta; expressed sequence AI528527;
General Note Trans heterozgous mice (Itgb2tm1Bay / Itgb2tm2Bay) on a mixed background that includes the strain PL/J and trans heterozygous mice on a congenic PL/J background display Phenotypic Similarity to Human Syndrome: Psoriasis. However, mice on backgrounds that do not include PL/J do not display psoriasis. (J:32126, J:84126)
Molecular Note A neomycin cassette disrupted the 5' boundary of exon 3. No protein was synthesized from this allele. [MGI Ref ID J:48542]

Genotyping

Genotyping Information

Genotyping Protocols

Itgb2tm2Bay, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Scharffetter-Kochanek K; Lu H; Norman K; van Nood N; Munoz F; Grabbe S; McArthur M; Lorenzo I; Kaplan S; Ley K; Smith CW; Montgomery CA; Rich S; Beaudet AL. 1998. Spontaneous skin ulceration and defective T cell function in CD18 null mice. J Exp Med 188(1):119-31. [PubMed: 9653089]  [MGI Ref ID J:48542]

Additional References

Wilson RW; Ballantyne CM; Smith CW; Montgomery C; Bradley A; O'Brien WE; Beaudet AL. 1993. Gene targeting yields a CD18-mutant mouse for study of inflammation. J Immunol 151(3):1571-8. [PubMed: 8101543]  [MGI Ref ID J:13599]

Itgb2tm2Bay related

Anderson KE; Boyle KB; Davidson K; Chessa TA; Kulkarni S; Jarvis GE; Sindrilaru A; Scharffetter-Kochanek K; Rausch O; Stephens LR; Hawkins PT. 2008. CD18-dependent activation of the neutrophil NADPH oxidase during phagocytosis of Escherichia coli or Staphylococcus aureus is regulated by class III but not class I or II PI3Ks. Blood 112(13):5202-11. [PubMed: 18755982]  [MGI Ref ID J:143800]

Balkow S; Heinz S; Schmidbauer P; Kolanus W; Holzmann B; Grabbe S; Laschinger M. 2010. LFA-1 activity state on dendritic cells regulates contact duration with T cells and promotes T-cell priming. Blood 116(11):1885-94. [PubMed: 20530790]  [MGI Ref ID J:164518]

Banerjee ER; Jiang Y; Henderson WR Jr; Scott LM; Papayannopoulou T. 2007. Alpha4 and beta2 integrins have nonredundant roles for asthma development, but for optimal allergen sensitization only alpha4 is critical. Exp Hematol 35(4):605-17. [PubMed: 17379071]  [MGI Ref ID J:123141]

Barlow SC; Collins RG; Ball NJ; Weaver CT; Schoeb TR; Bullard DC. 2003. Psoriasiform Dermatitis Susceptibility in Itgb2(tm1Bay) PL/J Mice Requires Low-Level CD18 Expression and at Least Two Additional Loci for Progression to Severe Disease. Am J Pathol 163(1):197-202. [PubMed: 12819024]  [MGI Ref ID J:84126]

Barlow SC; Langston W; Matthews KM; Chidlow JH Jr; Kevil CG. 2004. CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes. Am J Pathol 165(6):1849-52. [PubMed: 15579429]  [MGI Ref ID J:94971]

Berberich S; Dahne S; Schippers A; Peters T; Muller W; Kremmer E; Forster R; Pabst O. 2008. Differential molecular and anatomical basis for B cell migration into the peritoneal cavity and omental milky spots. J Immunol 180(4):2196-203. [PubMed: 18250426]  [MGI Ref ID J:132002]

Bonig H; Priestley GV; Oehler V; Papayannopoulou T. 2007. Hematopoietic progenitor cells (HPC) from mobilized peripheral blood display enhanced migration and marrow homing compared to steady-state bone marrow HPC. Exp Hematol 35(2):326-34. [PubMed: 17258081]  [MGI Ref ID J:123189]

Borjesson DL; Simon SI; Hodzic E; DeCock HE; Ballantyne CM; Barthold SW. 2003. Roles of neutrophil beta 2 integrins in kinetics of bacteremia, extravasation, and tick acquisition of Anaplasma phagocytophila in mice. Blood 101(8):3257-64. [PubMed: 12480703]  [MGI Ref ID J:82941]

Bowden RA; Ding ZM; Donnachie EM; Petersen TK; Michael LH; Ballantyne CM; Burns AR. 2002. Role of alpha4 integrin and VCAM-1 in CD18-independent neutrophil migration across mouse cardiac endothelium. Circ Res 90(5):562-9. [PubMed: 11909820]  [MGI Ref ID J:109755]

Boyle KB; Gyori D; Sindrilaru A; Scharffetter-Kochanek K; Taylor PR; Mocsai A; Stephens LR; Hawkins PT. 2011. Class IA Phosphoinositide 3-Kinase {beta} and {delta} Regulate Neutrophil Oxidase Activation in Response to Aspergillus fumigatus Hyphae. J Immunol 186(5):2978-89. [PubMed: 21257963]  [MGI Ref ID J:169412]

Carbo C; Duerschmied D; Goerge T; Hattori H; Sakai J; Cifuni SM; White GC 2nd; Chrzanowska-Wodnicka M; Luo HR; Wagner DD. 2010. Integrin-independent role of CalDAG-GEFI in neutrophil chemotaxis. J Leukoc Biol 88(2):313-9. [PubMed: 20413728]  [MGI Ref ID J:163946]

Chavakis E; Aicher A; Heeschen C; Sasaki K; Kaiser R; El Makhfi N; Urbich C; Peters T; Scharffetter-Kochanek K; Zeiher AM; Chavakis T; Dimmeler S. 2005. Role of beta2-integrins for homing and neovascularization capacity of endothelial progenitor cells. J Exp Med 201(1):63-72. [PubMed: 15623573]  [MGI Ref ID J:95265]

Chiriac MT; Roesler J; Sindrilaru A; Scharffetter-Kochanek K; Zillikens D; Sitaru C. 2007. NADPH oxidase is required for neutrophil-dependent autoantibody-induced tissue damage. J Pathol 212(1):56-65. [PubMed: 17380558]  [MGI Ref ID J:122096]

Coxon A; Cullere X; Knight S; Sethi S; Wakelin MW; Stavrakis G; Luscinskas FW; Mayadas TN. 2001. Fc gamma RIII mediates neutrophil recruitment to immune complexes. a mechanism for neutrophil accumulation in immune-mediated inflammation. Immunity 14(6):693-704. [PubMed: 11420040]  [MGI Ref ID J:78297]

Ding ZM; Babensee JE; Simon SI; Lu H; Perrard JL; Bullard DC; Dai XY; Bromley SK; Dustin ML; Entman ML; Smith CW; Ballantyne CM. 1999. Relative contribution of LFA-1 and Mac-1 to neutrophil adhesion and migration. J Immunol 163(9):5029-38. [PubMed: 10528208]  [MGI Ref ID J:83226]

Dorn T; Kuhn U; Bungartz G; Stiller S; Bauer M; Ellwart J; Peters T; Scharffetter-Kochanek K; Semmrich M; Laschinger M; Holzmann B; Klinkert WE; Straten PT; Kollgaard T; Sixt M; Brakebusch C. 2007. RhoH is important for positive thymocyte selection and T-cell receptor signaling. Blood 109(6):2346-55. [PubMed: 17119112]  [MGI Ref ID J:143161]

Dunne JL; Ballantyne CM; Beaudet AL; Ley K. 2002. Control of leukocyte rolling velocity in TNF-alpha-induced inflammation by LFA-1 and Mac-1. Blood 99(1):336-41. [PubMed: 11756189]  [MGI Ref ID J:73662]

Forlow SB; Foley PL; Ley K. 2002. Severely reduced neutrophil adhesion and impaired host defense against fecal and commensal bacteria in CD18-/-P-selectin-/- double null mice. FASEB J 16(12):1488-96. [PubMed: 12374771]  [MGI Ref ID J:120011]

Forlow SB; White EJ; Barlow SC; Feldman SH; Lu H; Bagby GJ; Beaudet AL; Bullard DC; Ley K. 2000. Severe inflammatory defect and reduced viability in CD18 and E-selectin double-mutant mice J Clin Invest 106(12):1457-66. [PubMed: 11120753]  [MGI Ref ID J:66426]

Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587]  [MGI Ref ID J:131154]

Glawe JD; Patrick DR; Huang M; Sharp CD; Barlow SC; Kevil CG. 2009. Genetic deficiency of Itgb2 or ItgaL prevents autoimmune diabetes through distinctly different mechanisms in NOD/LtJ mice. Diabetes 58(6):1292-301. [PubMed: 19223596]  [MGI Ref ID J:154351]

Goebel S; Huang M; Davis WC; Jennings M; Siahaan TJ; Alexander JS; Kevil CG. 2006. VEGF-A stimulation of leukocyte adhesion to colonic microvascular endothelium: implications for inflammatory bowel disease. Am J Physiol Gastrointest Liver Physiol 290(4):G648-54. [PubMed: 16293653]  [MGI Ref ID J:109115]

Gomez JC; Doerschuk CM. 2010. The role of CD18 in the production and release of neutrophils from the bone marrow. Lab Invest 90(4):599-610. [PubMed: 20142805]  [MGI Ref ID J:158612]

Gomez JC; Soltys J; Okano K; Dinauer MC; Doerschuk CM. 2008. The role of Rac2 in regulating neutrophil production in the bone marrow and circulating neutrophil counts. Am J Pathol 173(2):507-17. [PubMed: 18583316]  [MGI Ref ID J:138295]

Gorina R; Lyck R; Vestweber D; Engelhardt B. 2014. beta2 integrin-mediated crawling on endothelial ICAM-1 and ICAM-2 is a prerequisite for transcellular neutrophil diapedesis across the inflamed blood-brain barrier. J Immunol 192(1):324-37. [PubMed: 24259506]  [MGI Ref ID J:207099]

Grabbe S; Varga G; Beissert S; Steinert M; Pendl G; Seeliger S; Bloch W; Peters T; Schwarz T; Sunderkotter C; Scharffetter-Kochanek K. 2002. Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest 109(2):183-92. [PubMed: 11805130]  [MGI Ref ID J:74339]

Ho-Tin-Noe B; Carbo C; Demers M; Cifuni SM; Goerge T; Wagner DD. 2009. Innate immune cells induce hemorrhage in tumors during thrombocytopenia. Am J Pathol 175(4):1699-708. [PubMed: 19729481]  [MGI Ref ID J:153057]

Horie Y; Wolf R; Anderson DC; Granger DN. 1997. Hepatic leukostasis and hypoxic stress in adhesion molecule-deficient mice after gut ischemia/reperfusion. J Clin Invest 99(4):781-8. [PubMed: 9045883]  [MGI Ref ID J:39161]

Jakus Z; Simon E; Frommhold D; Sperandio M; Mocsai A. 2009. Critical role of phospholipase Cgamma2 in integrin and Fc receptor-mediated neutrophil functions and the effector phase of autoimmune arthritis. J Exp Med 206(3):577-93. [PubMed: 19273622]  [MGI Ref ID J:147080]

Jung U; Norman KE; Scharffetter-Kochanek K; Beaudet AL; Ley K. 1998. Transit time of leukocytes rolling through venules controls cytokine-induced inflammatory cell recruitment in vivo. J Clin Invest 102(8):1526-33. [PubMed: 9788965]  [MGI Ref ID J:115240]

Kandula S; Abraham C. 2004. LFA-1 on CD4+ T cells is required for optimal antigen-dependent activation in vivo. J Immunol 173(7):4443-51. [PubMed: 15383575]  [MGI Ref ID J:93734]

Kempf T; Zarbock A; Widera C; Butz S; Stadtmann A; Rossaint J; Bolomini-Vittori M; Korf-Klingebiel M; Napp LC; Hansen B; Kanwischer A; Bavendiek U; Beutel G; Hapke M; Sauer MG; Laudanna C; Hogg N; Vestweber D; Wollert KC. 2011. GDF-15 is an inhibitor of leukocyte integrin activation required for survival after myocardial infarction in mice. Nat Med 17(5):581-8. [PubMed: 21516086]  [MGI Ref ID J:171576]

Kess D; Peters T; Zamek J; Wickenhauser C; Tawadros S; Loser K; Varga G; Grabbe S; Nischt R; Sunderkotter C; Muller W; Krieg T; Scharffetter-Kochanek K. 2003. CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J Immunol 171(11):5697-706. [PubMed: 14634077]  [MGI Ref ID J:109598]

Kevil CG; Hicks MJ; He X; Zhang J; Ballantyne CM; Raman C; Schoeb TR; Bullard DC. 2004. Loss of LFA-1, but not Mac-1, protects MRL/MpJ-Fas(lpr) mice from autoimmune disease. Am J Pathol 165(2):609-16. [PubMed: 15277234]  [MGI Ref ID J:91523]

Kunkel EJ; Dunne JL; Ley K. 2000. Leukocyte arrest during cytokine-dependent inflammation in vivo. J Immunol 164(6):3301-8. [PubMed: 10706723]  [MGI Ref ID J:112234]

Lammermann T; Bader BL; Monkley SJ; Worbs T; Wedlich-Soldner R; Hirsch K; Keller M; Forster R; Critchley DR; Fassler R; Sixt M. 2008. Rapid leukocyte migration by integrin-independent flowing and squeezing. Nature 453(7191):51-5. [PubMed: 18451854]  [MGI Ref ID J:134784]

Lee SH; Prince JE; Rais M; Kheradmand F; Shardonofsky F; Lu H; Beaudet AL; Smith CW; Soong L; Corry DB. 2003. Differential requirement for CD18 in T-helper effector homing. Nat Med 9(10):1281-6. [PubMed: 14502280]  [MGI Ref ID J:99598]

Lefevre L; Lugo-Villarino G; Meunier E; Valentin A; Olagnier D; Authier H; Duval C; Dardenne C; Bernad J; Lemesre JL; Auwerx J; Neyrolles O; Pipy B; Coste A. 2013. The C-type lectin receptors dectin-1, MR, and SIGNR3 contribute both positively and negatively to the macrophage response to Leishmania infantum. Immunity 38(5):1038-49. [PubMed: 23684988]  [MGI Ref ID J:203147]

Li Y; Muruve DA; Collins RG; Lee SS; Kubes P. 2002. The role of selectins and integrins in adenovirus vector-induced neutrophil recruitment to the liver. Eur J Immunol 32(12):3443-52. [PubMed: 12442326]  [MGI Ref ID J:80852]

Li Z; Burns AR; Smith CW. 2006. Lymphocyte function-associated antigen-1-dependent inhibition of corneal wound healing. Am J Pathol 169(5):1590-600. [PubMed: 17071583]  [MGI Ref ID J:114562]

Li Z; Burns AR; Smith CW. 2006. Two waves of neutrophil emigration in response to corneal epithelial abrasion: distinct adhesion molecule requirements. Invest Ophthalmol Vis Sci 47(5):1947-55. [PubMed: 16639002]  [MGI Ref ID J:108504]

Li Z; Rumbaut RE; Burns AR; Smith CW. 2006. Platelet response to corneal abrasion is necessary for acute inflammation and efficient re-epithelialization. Invest Ophthalmol Vis Sci 47(11):4794-802. [PubMed: 17065490]  [MGI Ref ID J:123092]

Liang Y; Liu C; Djeu JY; Zhong B; Peters T; Scharffetter-Kochanek K; Anasetti C; Yu XZ. 2008. Beta2 integrins separate graft-versus-host disease and graft-versus-leukemia effects. Blood 111(2):954-62. [PubMed: 17928532]  [MGI Ref ID J:130082]

Lo CG; Xu Y; Proia RL; Cyster JG. 2005. Cyclical modulation of sphingosine-1-phosphate receptor 1 surface expression during lymphocyte recirculation and relationship to lymphoid organ transit. J Exp Med 201(2):291-301. [PubMed: 15657295]  [MGI Ref ID J:95700]

Marski M; Ye AL; Abraham C. 2007. CD18 is required for intestinal T cell responses at multiple immune checkpoints. J Immunol 178(4):2104-12. [PubMed: 17277114]  [MGI Ref ID J:143996]

Miura Y; Miura M; Gronthos S; Allen MR; Cao C; Uveges TE; Bi Y; Ehirchiou D; Kortesidis A; Shi S; Zhang L. 2005. Defective osteogenesis of the stromal stem cells predisposes CD18-null mice to osteoporosis. Proc Natl Acad Sci U S A 102(39):14022-7. [PubMed: 16172402]  [MGI Ref ID J:101407]

Mizgerd JP; Bullard DC; Hicks MJ; Beaudet AL; Doerschuk CM. 1999. Chronic inflammatory disease alters adhesion molecule requirements for acute neutrophil emigration in mouse skin. J Immunol 162(9):5444-8. [PubMed: 10228023]  [MGI Ref ID J:112286]

Mizgerd JP; Horwitz BH; Quillen HC; Scott ML; Doerschuk CM. 1999. Effects of CD18 deficiency on the emigration of murine neutrophils during pneumonia. J Immunol 163(2):995-9. [PubMed: 10395697]  [MGI Ref ID J:56153]

Mizgerd JP; Kubo H; Kutkoski GJ; Bhagwan SD; Scharffetter-Kochanek K; Beaudet AL; Doerschuk CM. 1997. Neutrophil emigration in the skin, lungs, and peritoneum: different requirements for CD11/CD18 revealed by CD18-deficient mice. J Exp Med 186(8):1357-64. [PubMed: 9334375]  [MGI Ref ID J:93674]

Mocsai A; Abram CL; Jakus Z; Hu Y; Lanier LL; Lowell CA. 2006. Integrin signaling in neutrophils and macrophages uses adaptors containing immunoreceptor tyrosine-based activation motifs. Nat Immunol 7(12):1326-33. [PubMed: 17086186]  [MGI Ref ID J:116120]

Mueller H; Stadtmann A; Van Aken H; Hirsch E; Wang D; Ley K; Zarbock A. 2010. Tyrosine kinase Btk regulates E-selectin-mediated integrin activation and neutrophil recruitment by controlling phospholipase C (PLC) gamma2 and PI3Kgamma pathways. Blood 115(15):3118-27. [PubMed: 20167705]  [MGI Ref ID J:160787]

Nakazawa T; Hisatomi T; Nakazawa C; Noda K; Maruyama K; She H; Matsubara A; Miyahara S; Nakao S; Yin Y; Benowitz L; Hafezi-Moghadam A; Miller JW. 2007. Monocyte chemoattractant protein 1 mediates retinal detachment-induced photoreceptor apoptosis. Proc Natl Acad Sci U S A 104(7):2425-30. [PubMed: 17284607]  [MGI Ref ID J:119752]

Nemeth T; Futosi K; Hably C; Brouns MR; Jakob SM; Kovacs M; Kertesz Z; Walzog B; Settleman J; Mocsai A. 2010. Neutrophil Functions and Autoimmune Arthritis in the Absence of p190RhoGAP: Generation and Analysis of a Novel Null Mutation in Mice. J Immunol 185(5):3064-75. [PubMed: 20675588]  [MGI Ref ID J:162994]

Oreshkova T; Wang H; Seier AM; Sindrilaru A; Varga G; Grabbe S; Scharffetter-Kochanek K; Peters T. 2009. Beta(2) integrin deficiency yields unconventional double-negative T cells distinct from mature classical natural killer T cells in mice. Immunology 128(2):271-86. [PubMed: 19740384]  [MGI Ref ID J:162288]

Ostanin DV; Furr KL; Pavlick KP; Gray L; Kevil CG; Shukla D; D'Souza D; Hoffman JM; Grisham MB. 2007. T cell-associated CD18 but not CD62L, ICAM-1, or PSGL-1 is required for the induction of chronic colitis. Am J Physiol Gastrointest Liver Physiol 292(6):G1706-14. [PubMed: 17332469]  [MGI Ref ID J:123696]

Pabst O; Peters T; Czeloth N; Bernhardt G; Scharffetter-Kochanek K; Forster R. 2005. Cutting edge: egress of newly generated plasma cells from peripheral lymph nodes depends on beta 2 integrin. J Immunol 174(12):7492-5. [PubMed: 15944247]  [MGI Ref ID J:100795]

Papayannopoulou T; Priestley GV; Nakamoto B; Zafiropoulos V; Scott LM. 2001. Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins. Blood 98(8):2403-11. [PubMed: 11588037]  [MGI Ref ID J:115624]

Papayannopoulou T; Priestley GV; Nakamoto B; Zafiropoulos V; Scott LM; Harlan JM. 2001. Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice. Blood 97(5):1282-8. [PubMed: 11222371]  [MGI Ref ID J:67767]

Peters T; Bloch W; Wickenhauser C; Tawadros S; Oreshkova T; Kess D; Krieg T; Muller W; Scharffetter-Kochanek K. 2006. Terminal B cell differentiation is skewed by deregulated interleukin-6 secretion in beta2 integrin-deficient mice. J Leukoc Biol 80(3):599-607. [PubMed: 16844762]  [MGI Ref ID J:112667]

Peters T; Sindrilaru A; Hinz B; Hinrichs R; Menke A; Al-Azzeh EA; Holzwarth K; Oreshkova T; Wang H; Kess D; Walzog B; Sulyok S; Sunderkotter C; Friedrich W; Wlaschek M; Krieg T; Scharffetter-Kochanek K. 2005. Wound-healing defect of CD18(-/-) mice due to a decrease in TGF-beta1 and myofibroblast differentiation. EMBO J 24(19):3400-10. [PubMed: 16148944]  [MGI Ref ID J:126204]

Petrescu MS; Larry CL; Bowden RA; Williams GW; Gagen D; Li Z; Smith CW; Burns AR. 2007. Neutrophil interactions with keratocytes during corneal epithelial wound healing: a role for CD18 integrins. Invest Ophthalmol Vis Sci 48(11):5023-9. [PubMed: 17962453]  [MGI Ref ID J:127159]

Piguet PF; Vesin C; Rochat A. 2001. Beta2 integrin modulates platelet caspase activation and life span in mice. Eur J Cell Biol 80(2):171-7. [PubMed: 11302522]  [MGI Ref ID J:102596]

Planck SR; Han YB; Park JM; O'Rourke L; Gutierrez-Ramos JC; Rosenbaum JT. 1998. The effect of genetic deficiency of adhesion molecules on the course of endotoxin-induced uveitis. Curr Eye Res 17(9):941-6. [PubMed: 9746442]  [MGI Ref ID J:114206]

Quast T; Tappertzhofen B; Schild C; Grell J; Czeloth N; Forster R; Alon R; Fraemohs L; Dreck K; Weber C; Lammermann T; Sixt M; Kolanus W. 2009. Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells. Blood 113(23):5801-10. [PubMed: 19346499]  [MGI Ref ID J:149500]

Ren B; McCrory MA; Pass C; Bullard DC; Ballantyne CM; Xu Y; Briles DE; Szalai AJ. 2004. The virulence function of Streptococcus pneumoniae surface protein A involves inhibition of complement activation and impairment of complement receptor-mediated protection. J Immunol 173(12):7506-12. [PubMed: 15585877]  [MGI Ref ID J:94855]

Rosetti F; Tsuboi N; Chen K; Nishi H; Ernandez T; Sethi S; Croce K; Stavrakis G; Alcocer-Varela J; Gomez-Martin D; van Rooijen N; Kyttaris VC; Lichtman AH; Tsokos GC; Mayadas TN. 2012. Human lupus serum induces neutrophil-mediated organ damage in mice that is enabled by Mac-1 deficiency. J Immunol 189(7):3714-23. [PubMed: 22933624]  [MGI Ref ID J:190352]

Saylor CA; Dadachova E; Casadevall A. 2010. Murine IgG1 and IgG3 isotype switch variants promote phagocytosis of Cryptococcus neoformans through different receptors. J Immunol 184(1):336-43. [PubMed: 19949107]  [MGI Ref ID J:159001]

Schmidt S; Nakchbandi I; Ruppert R; Kawelke N; Hess MW; Pfaller K; Jurdic P; Fassler R; Moser M. 2011. Kindlin-3-mediated signaling from multiple integrin classes is required for osteoclast-mediated bone resorption. J Cell Biol 192(5):883-97. [PubMed: 21357746]  [MGI Ref ID J:170685]

Schumann K; Lammermann T; Bruckner M; Legler DF; Polleux J; Spatz JP; Schuler G; Forster R; Lutz MB; Sorokin L; Sixt M. 2010. Immobilized chemokine fields and soluble chemokine gradients cooperatively shape migration patterns of dendritic cells. Immunity 32(5):703-13. [PubMed: 20471289]  [MGI Ref ID J:160695]

Schymeinsky J; Gerstl R; Mannigel I; Niedung K; Frommhold D; Panthel K; Heesemann J; Sixt M; Quast T; Kolanus W; Mocsai A; Wienands J; Sperandio M; Walzog B. 2009. A fundamental role of mAbp1 in neutrophils: impact on beta(2) integrin-mediated phagocytosis and adhesion in vivo. Blood 114(19):4209-20. [PubMed: 19638624]  [MGI Ref ID J:154208]

Schymeinsky J; Sindrilaru A; Frommhold D; Sperandio M; Gerstl R; Then C; Mocsai A; Scharffetter-Kochanek K; Walzog B. 2006. The Vav binding site of the non-receptor tyrosine kinase Syk at Tyr 348 is critical for beta2 integrin (CD11/CD18)-mediated neutrophil migration. Blood 108(12):3919-27. [PubMed: 16882714]  [MGI Ref ID J:140446]

Shi S; Blumenthal A; Hickey CM; Gandotra S; Levy D; Ehrt S. 2005. Expression of many immunologically important genes in Mycobacterium tuberculosis-infected macrophages is independent of both TLR2 and TLR4 but dependent on IFN-alphabeta receptor and STAT1. J Immunol 175(5):3318-28. [PubMed: 16116224]  [MGI Ref ID J:113220]

Sindrilaru A; Seeliger S; Ehrchen JM; Peters T; Roth J; Scharffetter-Kochanek K; Sunderkotter CH. 2007. Site of blood vessel damage and relevance of CD18 in a murine model of immune complex-mediated vasculitis. J Invest Dermatol 127(2):447-54. [PubMed: 17008881]  [MGI Ref ID J:117633]

Smith E; Stark MA; Zarbock A; Burcin TL; Bruce AC; Vaswani D; Foley P; Ley K. 2008. IL-17A inhibits the expansion of IL-17A-producing T cells in mice through 'short-loop' inhibition via IL-17 receptor. J Immunol 181(2):1357-64. [PubMed: 18606690]  [MGI Ref ID J:137660]

Smith E; von Vietinghoff S; Stark MA; Zarbock A; Sanders JM; Duley A; Rivera-Nieves J; Bender TP; Ley K. 2009. T-lineage cells require the thymus but not VDJ recombination to produce IL-17A and regulate granulopoiesis in vivo. J Immunol 183(9):5685-93. [PubMed: 19843951]  [MGI Ref ID J:156790]

Soloviev DA; Jawhara S; Fonzi WA. 2011. Regulation of Innate Immune Response to Candida albicans Infections by {alpha}M{beta}2-Pra1p Interaction. Infect Immun 79(4):1546-58. [PubMed: 21245270]  [MGI Ref ID J:170622]

Stark MA; Huo Y; Burcin TL; Morris MA; Olson TS; Ley K. 2005. Phagocytosis of Apoptotic Neutrophils Regulates Granulopoiesis via IL-23 and IL-17. Immunity 22(3):285-94. [PubMed: 15780986]  [MGI Ref ID J:97026]

Takanaga H; Mackenzie B; Suzuki Y; Hediger MA. 2005. Identification of mammalian proline transporter SIT1 (SLC6A20) with characteristics of classical system imino. J Biol Chem 280(10):8974-84. [PubMed: 15632147]  [MGI Ref ID J:97793]

Ulyanova T; Priestley GV; Banerjee ER; Papayannopoulou T. 2007. Unique and redundant roles of alpha4 and beta2 integrins in kinetics of recruitment of lymphoid vs myeloid cell subsets to the inflamed peritoneum revealed by studies of genetically deficient mice. Exp Hematol 35(8):1256-65. [PubMed: 17553614]  [MGI Ref ID J:126483]

Vachon E; Martin R; Kwok V; Cherepanov V; Chow CW; Doerschuk CM; Plumb J; Grinstein S; Downey GP. 2007. CD44-mediated phagocytosis induces inside-out activation of complement receptor-3 in murine macrophages. Blood 110(13):4492-502. [PubMed: 17827392]  [MGI Ref ID J:149095]

Varga G; Nippe N; Balkow S; Peters T; Wild MK; Seeliger S; Beissert S; Krummen M; Roth J; Sunderkotter C; Grabbe S. 2010. LFA-1 contributes to signal I of T-cell activation and to the production of T(h)1 cytokines. J Invest Dermatol 130(4):1005-12. [PubMed: 20072134]  [MGI Ref ID J:159935]

Wang JX; Bair AM; King SL; Shnayder R; Huang YF; Shieh CC; Soberman RJ; Fuhlbrigge RC; Nigrovic PA. 2012. Ly6G ligation blocks recruitment of neutrophils via a beta2-integrin-dependent mechanism. Blood 120(7):1489-98. [PubMed: 22661700]  [MGI Ref ID J:189105]

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Weinmann P; Scharffetter-Kochanek K; Forlow SB; Peters T; Walzog B. 2003. A role for apoptosis in the control of neutrophil homeostasis in the circulation: insights from CD18-deficient mice. Blood 101(2):739-46. [PubMed: 12393639]  [MGI Ref ID J:115556]

Wethmar K; Helmus Y; Luhn K; Jones C; Laskowska A; Varga G; Grabbe S; Lyck R; Engelhardt B; Bixel MG; Butz S; Loser K; Beissert S; Ipe U; Vestweber D; Wild MK. 2006. Migration of immature mouse DC across resting endothelium is mediated by ICAM-2 but independent of beta2-integrins and murine DC-SIGN homologues. Eur J Immunol 36(10):2781-94. [PubMed: 16981228]  [MGI Ref ID J:118093]

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Health & husbandry

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Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

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Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   See control note: Wildtype mice from the colony or C57BL/6J mice (Stock No. 000664) may be used as controls.
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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