Strain Name:

C57BL/6-Tg(Cd8aCd8b)3Erob/J

Stock Number:

003331

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating InvestigatorDr. Ellen Robey,   Univ of California, Berkeley

Description
Homozygous mice constitutively express CD8.1 under the control of the T cell-specific CD2 promoter. The mice are viable and fertile. CD8.1 differs from the CD8.2 allele expressed in the C57BL/6 mouse strain, allowing differentiation between the endogenous protein and the transgene product using monoclonal antibodies. This transgenic line was created to study the mechanisms of T cell selection and lineage commitment in the thymus.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of CD2     (6 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(Cd8aCd8b)3Erob/0

        involves: C57BL/6 * DBA/2
  • immune system phenotype
  • abnormal CD8-positive T cell differentiation
    • transgenic expression of the CD8 complex leads to almost all peripheral T cells expressing CD8 either alone as CD8+ T cells or as double positive CD4+CD8+ T cells   (MGI Ref ID J:93004)
    • endogenous expression of CD8 genes does not occur in CD4+CD8+ T cells but does occur in single positive CD8+ T cells   (MGI Ref ID J:93004)
  • abnormal double-positive T cell morphology
    • the single-positive CD4 T cell population in the periphery is replaced by a CD4+CD8+ population   (MGI Ref ID J:93004)
    • the double-positive population in the thymus is greatly enhanced   (MGI Ref ID J:93004)
  • decreased CD4-positive T cell number
    • the single positive CD4 T cell population in the thymus and in the periphery is virtually absent due to transgenic expression of CD8   (MGI Ref ID J:93004)
  • decreased double-negative T cell number
    • the double negative population in the thymus is severely reduced due to expression of the transgenic CD8 complex   (MGI Ref ID J:93004)
  • increased CD8-positive T cell number
    • almost all peripheral T cells express CD8 with over half also expressing CD4   (MGI Ref ID J:93004)
  • hematopoietic system phenotype
  • abnormal CD8-positive T cell differentiation
    • transgenic expression of the CD8 complex leads to almost all peripheral T cells expressing CD8 either alone as CD8+ T cells or as double positive CD4+CD8+ T cells   (MGI Ref ID J:93004)
    • endogenous expression of CD8 genes does not occur in CD4+CD8+ T cells but does occur in single positive CD8+ T cells   (MGI Ref ID J:93004)
  • abnormal double-positive T cell morphology
    • the single-positive CD4 T cell population in the periphery is replaced by a CD4+CD8+ population   (MGI Ref ID J:93004)
    • the double-positive population in the thymus is greatly enhanced   (MGI Ref ID J:93004)
  • decreased CD4-positive T cell number
    • the single positive CD4 T cell population in the thymus and in the periphery is virtually absent due to transgenic expression of CD8   (MGI Ref ID J:93004)
  • decreased double-negative T cell number
    • the double negative population in the thymus is severely reduced due to expression of the transgenic CD8 complex   (MGI Ref ID J:93004)
  • increased CD8-positive T cell number
    • almost all peripheral T cells express CD8 with over half also expressing CD4   (MGI Ref ID J:93004)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Cd8aCd8b)3Erob
Allele Name transgene insertion 3, Ellen Robey
Allele Type Transgenic (Inserted expressed sequence)
Mutation Made ByDr. Ellen Robey,   Univ of California, Berkeley
Strain of Origin(C57BL/6 x DBA/2)F2
Expressed Gene Cd8a, CD8a molecule, rat
Promoter CD2, CD2 molecule, human
Molecular Note The murine Cd8a and Cd8b1 coding sequences were placed under the control of the T cell-specific CD2 promoter. Six copies of the transgene incorporated into the genome. The resulting heterodimer complex expressed was the "CD8.1" variant that differs from the "CD8.2" variant complex normally expressed in C57BL/6 mice. Transgenic expression was detected on almost all thymocytes and T cells as determined by flow cytometry using antibodies that can distinguish the two CD8 variants. [MGI Ref ID J:93004]
 
 

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Robey EA; Fowlkes BJ; Gordon JW; Kioussis D; von Boehmer H; Ramsdell F; Axel R. 1991. Thymic selection in CD8 transgenic mice supports an instructive model for commitment to a CD4 or CD8 lineage. Cell 64(1):99-107. [PubMed: 1898873]  [MGI Ref ID J:93004]

Additional References

Robey E; Itano A; Fanslow WC; Fowlkes BJ. 1994. Constitutive CD8 expression allows inefficient maturation of CD4+ helper T cells in class II major histocompatibility complex mutant mice. J Exp Med 179(6):1997-2004. [PubMed: 7515104]  [MGI Ref ID J:19021]

Tg(Cd8aCd8b)3Erob related

Ali M; Weinreich M; Balcaitis S; Cooper CJ; Fink PJ. 2003. Differential regulation of peripheral CD4+ T cell tolerance induced by deletion and TCR revision. J Immunol 171(11):6290-6. [PubMed: 14634147]  [MGI Ref ID J:132828]

Engel I; Hammond K; Sullivan BA; He X; Taniuchi I; Kappes D; Kronenberg M. 2010. Co-receptor choice by V alpha14i NKT cells is driven by Th-POK expression rather than avoidance of CD8-mediated negative selection. J Exp Med 207(5):1015-29. [PubMed: 20404101]  [MGI Ref ID J:161237]

Golde WT; Kappel KJ; Dequesne G; Feron C; Plainchamp D; Capiau C; Lobet Y. 1994. Tick transmission of Borrelia burgdorferi to inbred strains of mice induces an antibody response to P39 but not to outer surface protein A [published erratum appears in Infect Immun 1995 Mar;63(3):1146] Infect Immun 62(6):2625-7. [PubMed: 8188388]  [MGI Ref ID J:19024]

Robey E; Itano A; Fanslow WC; Fowlkes BJ. 1994. Constitutive CD8 expression allows inefficient maturation of CD4+ helper T cells in class II major histocompatibility complex mutant mice. J Exp Med 179(6):1997-2004. [PubMed: 7515104]  [MGI Ref ID J:19021]

Wack A; Coles M; Norton T; Hostert A; Kioussis D. 2000. Early onset of CD8 transgene expression inhibits the transition from DN3 to DP thymocytes. J Immunol 165(3):1236-42. [PubMed: 10903721]  [MGI Ref ID J:120481]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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