Strain Name: |
C57BL/6-Tg(LckNotch1)9Erob/J |
|---|---|
Stock Number: |
003332 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Lck; Notch1; Tg(LckNotch1)9Erob; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Ellen Robey, Univ of California, Berkeley Generation N?+3 Strain Description
C57BL/6-TgN(LckNotch1)9Erob mice are viable and fertile. They express an activated form of Notch under the control of the thymocyte specific Lck proximal promoter. Transgenic mice have a 10-fold increase in both absolute number and percentage of mature CD8 lineage thymocytes compared to non-transgenic littermates, with a corresponding decrease in CD4 lineage cells. Activated Notch does not affect the proliferative capacity or lifespan of immature CD4+CD8+ thymocytes, nor does it lead to inappropriate proliferation of mature thymocytes. Instead, activated Notch appears to create a bias towards CD8 T cell development while inhibiting CD4 T cell development. These mice are useful for studying the role of Notch in the CD4/CD8 lineage decision in thymocytes.
Gene & Allele Details
| Allele Symbol | Tg(LckNotch1)9Erob | ||
|---|---|---|---|
| Allele Name | transgene insertion 9, Ellen Robey | ||
| Mutation Made By | Ellen Robey, Univ of California, Berkeley | ||
| Strain of Origin | (C57BL/6 x CBA/J)F2 | ||
| Expressed Gene | Notch1, Notch gene homolog 1 (Drosophila), rat | ||
| Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory | ||
| General Note | Transgenic mice are viable and fertile. They have a 10-fold increase in both absolute number and percentage of mature CD8 lineage thymocytes compared to non-transgenic littermates, with a corresponding decrease in CD4 lineage cells. In these mice activated Notch does not affect the proliferative capacity or lifespan of immature CD4+CD8+ thymocytes, nor does it lead to inappropriate proliferation of mature thymocytes. Instead, activated Notch appears to create a bias towards CD8 T cell development while inhibiting CD4 T cell development. | ||
| Molecular Note | The transgene expresses an activated form of Notch under the control of the thymocyte specific Lck proximal promoter. [MGI Ref ID J:93005] | ||
Genotyping Protocols
Human Growth Hormone Polyadenylation Signal Sequence
Related Strains
Strains carrying other alleles of Lck
002817 B6.129S2-Lcktm1Mak/J 003802 B6.Cg-Tg(Lck-cre)548Jxm/J 002427 C3H/He-Tg(LCKprBCL2)36Sjk/J 002230 C57BL/6J-Tg(LckIl4)1315Dbl/J 005732 NOD.Cg-Tg(Lck-cre)548Jxm/AchJ View Strains carrying other alleles of Lck (5 strains)
References
Selected Reference(s)
Additional ReferencesRobey E; Chang D; Itano A; Cado D; Alexander H; Lans D; Weinmaster G; Salmon P. 1996. An activated form of Notch influences the choice between CD4 and CD8 T cell lineages. Cell 87(3):483-92. [PubMed: 8898201] [MGI Ref ID J:93005]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(LckNotch1)9Erob/J |
| Stock Number: | 003332 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
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