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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names FVB/N-Tg(ZP3A)7812Dean/J (Changed: 06-OCT-06 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+2p
Generation DefinitionsDonating Investigator Jurrien Dean, NIH
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
Tg(ZP3)7812Dean/?
involves: FVB/N
- reproductive system phenotype
- *normal* reproductive system phenotype
- litter sizes of transgenic female mice mated to wild type males are statistically no different from those of wild type females litter sizes of transgenic female mice mated to wild-type males are statistically no different from those of wild-type females (MGI Ref ID J:77459)
- although mouse sperm bind in vitro with similar avidity to cumulus-free ovulated oocytes from wild type and human ZP3 transgenic female mice, human sperm do not attach even transiently to either although mouse sperm bind in vitro with similar avidity to cumulus-free ovulated oocytes from wild-type and human ZP3 transgenic female mice, human sperm do not attach even transiently to either (MGI Ref ID J:77459)
This mouse can be used to support research in many areas including:
Reproductive Biology Research
Developmental Defects Affecting Gonads
Fertility Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(ZP3)7812Dean | ||
|---|---|---|---|
| Allele Name | transgene insertion 7812, Jurrien Dean | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TgN(HuZP3)3NIH; huZP3; | ||
| Mutation Made By | Jurrien Dean, NIH | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | ZP3, zona pellucida glycoprotein 3 (sperm receptor), human | ||
| Promoter | ZP3, zona pellucida glycoprotein 3 (sperm receptor), human | ||
| General Note | Three transgenic lines were established. This line and the line originally called TgN(HuZP3)2NIH each was estimated by Southern blot densitometry to carry 2 copies, and the line originally called TgN(HuZP3)1NIH to carry 4-6 copies of the transgene. Authors state that null-allele rescue results using the former two strains were identical. | ||
| Molecular Note | This transgene comprises 6 kb of 5' flanking DNA from the mouse zona pellucida 3 gene ligated to the 5' half of the human zona pellucida 3 gene, including exons and introns 1-4, followed by cDNA encoded by exons 5-8 of the human gene and the bovine growth hormone polyadenylation signal. This transgenic line was estimated by Southern blot densitometry to carry two copies of the transgene. Transgene expression was shown by RNase protection analysis of multiple tissues to be ovary-specific. In situ hybridization localized expression to the cytoplasm of growing oocytes, at a level similar to that of the co-expressed endogenous mouse gene. Species-specific antibodies detect lower levels of human and mouse ZP3 in zonae pellucidae of mature transgenic oocytes than in human or wild-type mouse zonae, respectively; the total (human + mouse) approximating that in normal mouse zonae. The molecular mass of the transgenic protein suggests it undergoes post-translational modification similarly to the native human protein. [MGI Ref ID J:77459] | ||
References
References provided by MGI
Selected Reference(s)
Rankin TL; Tong ZB; Castle PE; Lee E; Gore-Langton R; Nelson LM; Dean J. 1998. Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development 125(13):2415-24. [PubMed: 9609824] [MGI Ref ID J:77459]
Additional References
Tg(ZP3)7812Dean relatedRankin TL; Coleman JS; Epifano O; Hoodbhoy T; Turner SG; Castle PE; Lee E; Gore-Langton R; Dean J. 2003. Fertility and taxon-specific sperm binding persist after replacement of mouse sperm receptors with human homologs. Dev Cell 5(1):33-43. [PubMed: 12852850] [MGI Ref ID J:93482]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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