Description

Strain Information

Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse H2 Haplotype g7

Appearance
albino, pink eyed
Related Genotype: A/A Tyr^c/Tyr^c

Development
The Tg(B2M)55Hpl allele was generated in (CBA x C57BL/Lia)F2 fertilized eggs (Krimpenfort et al., 1987). In 1996 this allele was received at The Jackson Laboratory on a B10.D2 congenic background at N10F10. The Tg(B2M)55Hpl allele was then backcrossed onto NOD/Lt by David Serreze until N10 then intercrossed and made homozygous.

Control Information

	Control
	None Available
Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(B2M)55Hpl allele

003355	NOD.Cg-B2mtm1Unc Tg(B2M)55Hpl/Dvs
004548	NOD.Cg-B2mtm1Unc Tg(B2M)55Hpl Tg(HLA-A2.1)1Enge/DvsJ

View Strains carrying   Tg(B2M)55Hpl     (2 strains)

Strains carrying other alleles of B2M

003428	B6(Cg)-Tg(B2M)1Trg Tg(HLA-B)1Trg/Dcr
006611	NOD.129P2(B6)-B2mtm1Unc Tg(HLA-A/H2-D/B2M)1Dvs/DvsJ
006605	NOD.Cg-Prkdcscid Emv30b Tg(HLA-A/H2-D/B2M)1Dvs/DvsJ
004342	NOD.Cg-Tg(B2M)1Trg Tg(HLA-B)1Trg/Dvs
006604	NOD/ShiLtDvs-Tg(HLA-A/H2-D/B2M)1Dvs/J

View Strains carrying other alleles of B2M     (5 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(B2M)55Hpl
Allele Name		transgene insertion 55, Hidde Ploegh
Allele Type		Transgenic (random, expressed)
Strain of Origin		(CBA x C57BL/LiA)F2
Expressed Gene		B2M, beta-2-microglobulin, human
Molecular Note		A transgene containing the human B2M gene was shown to be expressed in spleen cells of mice carrying the transgene. [MGI Ref ID J:74416]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Krimpenfort P; Rudenko G; Hochstenbach F; Guessow D; Berns A; Ploegh H. 1987. Crosses of two independently derived transgenic mice demonstrate functional complementation of the genes encoding heavy (HLA-B27) and light (beta 2-microglobulin) chains of HLA class I antigens. EMBO J 6(6):1673-6. [PubMed: 3301331]  [MGI Ref ID J:74416]

Tg(B2M)55Hpl related

Banuelos SJ; Shultz LD; Greiner DL; Burzenski LM; Gott B; Lyons BL; Rossini AA; Appel MC. 2004. Rejection of human islets and human HLA-A2.1 transgenic mouse islets by alloreactive human lymphocytes in immunodeficient NOD-scid and NOD-Rag1(null)Prf1(null) mice. Clin Immunol 112(3):273-83. [PubMed: 15308121]  [MGI Ref ID J:91764]

Fletcher JM; Jordan MA; Snelgrove SL; Slattery RM; Dufour FD; Kyparissoudis K; Besra GS; Godfrey DI; Baxter AG. 2008. Congenic analysis of the NKT cell control gene Nkt2 implicates the peroxisomal protein Pxmp4. J Immunol 181(5):3400-12. [PubMed: 18714012]  [MGI Ref ID J:138945]

Hamilton-Williams EE; Serreze DV; Charlton B; Johnson EA; Marron MP; Mullbacher A; Slattery RM. 2001. Transgenic rescue implicates beta 2-microglobulin as a diabetes susceptibility gene in nonobese diabetic (NOD) mice. Proc Natl Acad Sci U S A 98(20):11533-8. [PubMed: 11572996]  [MGI Ref ID J:71837]

Kievits F; Ivanyi P; Krimpenfort P; Berns A; Ploegh HL. 1987. HLA-restricted recognition of viral antigens in HLA transgenic mice. Nature 329(6138):447-9. [PubMed: 2821399]  [MGI Ref ID J:128769]

Kievits F; Lokhorst W; Ivanyi P. 1990. Abnormal anti-viral immune response in mice is corrected in HLA-B27.2-transgenic mice. Eur J Immunol 20(5):1189-92. [PubMed: 1694133]  [MGI Ref ID J:128764]

Krimpenfort P; Rudenko G; Hochstenbach F; Guessow D; Berns A; Ploegh H. 1987. Crosses of two independently derived transgenic mice demonstrate functional complementation of the genes encoding heavy (HLA-B27) and light (beta 2-microglobulin) chains of HLA class I antigens. EMBO J 6(6):1673-6. [PubMed: 3301331]  [MGI Ref ID J:74416]

Marron MP; Graser RT; Chapman HD; Serreze DV. 2002. Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. Proc Natl Acad Sci U S A 99(21):13753-8. [PubMed: 12361980]  [MGI Ref ID J:109851]

Mezo AR; McDonnell KA; Hehir CA; Low SC; Palombella VJ; Stattel JM; Kamphaus GD; Fraley C; Zhang Y; Dumont JA; Bitonti AJ. 2008. Reduction of IgG in nonhuman primates by a peptide antagonist of the neonatal Fc receptor FcRn. Proc Natl Acad Sci U S A 105(7):2337-42. [PubMed: 18272495]  [MGI Ref ID J:132181]

Tishon A; LaFace DM; Lewicki H; van Binnendijk RS; Osterhaus A; Oldstone MB. 2000. Transgenic mice expressing human HLA and CD8 molecules generate HLA-restricted measles virus cytotoxic T lymphocytes of the same specificity as humans with natural measles virus infection. Virology 275(2):286-93. [PubMed: 10998329]  [MGI Ref ID J:127813]

Weinreich SS; Hoebe-Hewryk B; van der Horst AR; Boog CJP; Ivanyi P. 1997. The role of MHC class I heterodimer expression in mouse ankylosing enthesopathy. Immunogenetics 46(1):35-40. [PubMed: 9148786]  [MGI Ref ID J:41322]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

Control Information

	Control
	None Available
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.