Strain Name:

NOD.Cg-Tg(B2M)55Hpl/Dvs

Stock Number:

003356

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Availability:

Cryopreserved - Ready for recovery

Other products are available, see Purchasing Information for Cryopreserved Embryos

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
H2 Haplotypeg7

Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/Tyrc

Development
The Tg(B2M)55Hpl allele was generated in (CBA x C57BL/Lia)F2 fertilized eggs (Krimpenfort et al., 1987). In 1996 this allele was received at The Jackson Laboratory on a B10.D2 congenic background at N10F10. The Tg(B2M)55Hpl allele was then backcrossed onto NOD/Lt by David Serreze until N10 then intercrossed and made homozygous.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying   Tg(B2M)55Hpl     (3 strains)

View Strains carrying other alleles of B2M     (8 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Hypoproteinemia, Hypercatabolic   (B2M)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(B2M)55Hpl
Allele Name transgene insertion 55, Hidde Ploegh
Allele Type Transgenic (random, expressed)
Common Name(s) (B2M)55HplTG;
Strain of Origin(CBA x C57BL/LiA)F2
Expressed Gene B2M, beta-2-microglobulin, human
Promoter B2M, beta-2-microglobulin, human
Molecular Note A transgene containing the human B2M gene was shown to be expressed in spleen cells of mice carrying the transgene. [MGI Ref ID J:74416]
 

Genotyping

Genotyping Information

Genotyping Protocols

(B2M)55Hpl, QPCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Krimpenfort P; Rudenko G; Hochstenbach F; Guessow D; Berns A; Ploegh H. 1987. Crosses of two independently derived transgenic mice demonstrate functional complementation of the genes encoding heavy (HLA-B27) and light (beta 2-microglobulin) chains of HLA class I antigens. EMBO J 6(6):1673-6. [PubMed: 3301331]  [MGI Ref ID J:74416]

Tg(B2M)55Hpl related

Antal Z; Baker JC; Smith C; Jarchum I; Babad J; Mukherjee G; Yang Y; Sidney J; Sette A; Santamaria P; DiLorenzo TP. 2012. Beyond HLA-A*0201: new HLA-transgenic nonobese diabetic mouse models of type 1 diabetes identify the insulin C-peptide as a rich source of CD8+ T cell epitopes. J Immunol 188(11):5766-75. [PubMed: 22539795]  [MGI Ref ID J:188404]

Banuelos SJ; Shultz LD; Greiner DL; Burzenski LM; Gott B; Lyons BL; Rossini AA; Appel MC. 2004. Rejection of human islets and human HLA-A2.1 transgenic mouse islets by alloreactive human lymphocytes in immunodeficient NOD-scid and NOD-Rag1(null)Prf1(null) mice. Clin Immunol 112(3):273-83. [PubMed: 15308121]  [MGI Ref ID J:91764]

Fletcher JM; Jordan MA; Snelgrove SL; Slattery RM; Dufour FD; Kyparissoudis K; Besra GS; Godfrey DI; Baxter AG. 2008. Congenic analysis of the NKT cell control gene Nkt2 implicates the peroxisomal protein Pxmp4. J Immunol 181(5):3400-12. [PubMed: 18714012]  [MGI Ref ID J:138945]

Hamilton-Williams EE; Serreze DV; Charlton B; Johnson EA; Marron MP; Mullbacher A; Slattery RM. 2001. Transgenic rescue implicates beta 2-microglobulin as a diabetes susceptibility gene in nonobese diabetic (NOD) mice. Proc Natl Acad Sci U S A 98(20):11533-8. [PubMed: 11572996]  [MGI Ref ID J:71837]

Kievits F; Ivanyi P; Krimpenfort P; Berns A; Ploegh HL. 1987. HLA-restricted recognition of viral antigens in HLA transgenic mice. Nature 329(6138):447-9. [PubMed: 2821399]  [MGI Ref ID J:128769]

Kievits F; Lokhorst W; Ivanyi P. 1990. Abnormal anti-viral immune response in mice is corrected in HLA-B27.2-transgenic mice. Eur J Immunol 20(5):1189-92. [PubMed: 1694133]  [MGI Ref ID J:128764]

Krimpenfort P; Rudenko G; Hochstenbach F; Guessow D; Berns A; Ploegh H. 1987. Crosses of two independently derived transgenic mice demonstrate functional complementation of the genes encoding heavy (HLA-B27) and light (beta 2-microglobulin) chains of HLA class I antigens. EMBO J 6(6):1673-6. [PubMed: 3301331]  [MGI Ref ID J:74416]

Marron MP; Graser RT; Chapman HD; Serreze DV. 2002. Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. Proc Natl Acad Sci U S A 99(21):13753-8. [PubMed: 12361980]  [MGI Ref ID J:109851]

McPhee CG; Sproule TJ; Shin DM; Bubier JA; Schott WH; Steinbuck MP; Avenesyan L; Morse HC 3rd; Roopenian DC. 2011. MHC class I family proteins retard systemic lupus erythematosus autoimmunity and B cell lymphomagenesis. J Immunol 187(9):4695-704. [PubMed: 21964024]  [MGI Ref ID J:179430]

Mezo AR; McDonnell KA; Hehir CA; Low SC; Palombella VJ; Stattel JM; Kamphaus GD; Fraley C; Zhang Y; Dumont JA; Bitonti AJ. 2008. Reduction of IgG in nonhuman primates by a peptide antagonist of the neonatal Fc receptor FcRn. Proc Natl Acad Sci U S A 105(7):2337-42. [PubMed: 18272495]  [MGI Ref ID J:132181]

Tishon A; LaFace DM; Lewicki H; van Binnendijk RS; Osterhaus A; Oldstone MB. 2000. Transgenic mice expressing human HLA and CD8 molecules generate HLA-restricted measles virus cytotoxic T lymphocytes of the same specificity as humans with natural measles virus infection. Virology 275(2):286-93. [PubMed: 10998329]  [MGI Ref ID J:127813]

Weinreich SS; Hoebe-Hewryk B; van der Horst AR; Boog CJP; Ivanyi P. 1997. The role of MHC class I heterodimer expression in mouse ankylosing enthesopathy. Immunogenetics 46(1):35-40. [PubMed: 9148786]  [MGI Ref ID J:41322]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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