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- Disease & phenotype
- Genes & alleles
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;129S-H2dlAb1-Ea/J (Changed: 20-SEP-07 ) Type Deletion; Additional information on Mice with Chromosomal Aberrations. Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F11+N1p (28-MAR-04)
Generation DefinitionsDonating Investigator Christophe Benoist, Joslin Diabetes Center Appearance
white-bellied agouti
Related Genotype: Aw/?
black
Related Genotype: a/aDescription
Mice that are homozygous null for MHC class II genes H2-Ab1, H2-Aa, H2-Eb1, H2-Eb2, H2-Ea are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. MHC class II gene products (mRNA or protein) are not detected. A dramatic decrease is observed in the number of CD4 positive T cells in thymus, spleen and lymph nodes. This strain should serve as a suitable recipient of xenogenic Class II MHC transgenes allowing the engineering of mouse models of human MHC Class II-associated diseases.Development
This strain was developed in the laboratory of Dr. Christophe Benoist at IGBMC, Institut de Genetique et de Biologie Moleculaire et Cellulaire, Strasbourg. A 78.8 kb deletion disrupting Class II MHC genes was induced in 129S2/SvPas-derived H1 embryonic stem (ES) cells via Cre recombination. A hygro-resistance cassette was inserted at the deletion site. The deletion spans from the second exon of the H2-Ab1 gene the third exon of the H2-Ea gene. The H2-Aa, H2-Eb1 and H2-Eb2 genes are completely deleted. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were bred to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
Related Strains
Deletion
005535 B6.129S7-Del(11Cops3-Rnf112)1Jrl/J 005654 B6C3-Del(16Cbr1-ORF9)1Rhr/J 002802 C3.BLiA Pde6b+-Krd/J 004406 C3HeB/FeJ-Pou3f4del-J/J 002142 STOCK 11R30m/J 004711 STOCK Ednrbs-52Pub View Deletion (6 strains)
003584 B6.129S2-H2dlAb1-Ea/J
Phenotype
Phenotype Information
assigned by genotype
H2dlAb1-Ea/H2dlAb1-Ea
involves: 129S2/SvPas * C57BL/6
- immune system phenotype
- *normal* immune system phenotype
- stimulated dendritic cells produce normal amounts of IL-12 and TNF-alpha (MGI Ref ID J:157516)
- hematopoietic system phenotype
- abnormal T cell number (MGI Ref ID J:57484)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
H2dlAb1-Ea/H2dlAb1-Ea
B6.129S2-H2dlAb1-Ea/J
- mortality/aging
- increased sensitivity to induced morbidity/mortality
- following infection with either a low or high dose of IOE, mice succumb to the infection at 11 to 15 days and 7 to 9 days, respectively, compared to wild-type mice, which succumb at 14 to 17 days and 8 to 12 days, respectively (MGI Ref ID J:123934)
- immune system phenotype
- increased susceptibility to bacterial infection
- mice are more susceptible to infection with a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE) than wild-type mice (MGI Ref ID J:123934)
- following infection with either a low or high dose of IOE, mice succumb to the infection at 11 to 15 days and 7 to 9 days, respectively, compared to wild-type mice, which succumb at 14 to 17 days and 8 to 12 days, respectively (MGI Ref ID J:123934)
- mice have higher burdens of Ehrlichia bacteria in all organs following infection than do wild-type mice (MGI Ref ID J:123934)
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
MHC class II deficient
Lymphoid Tissue Defects
Internal/Organ Research
Lymphoid Tissue Defects
Research Tools
Immunology and Inflammation Research
MHC class II defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | H2dlAb1-Ea | ||
|---|---|---|---|
| Allele Name | targeted deletion, H2 complex | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | IIdelta; MHC II KO; MHC II-; MHC II0; MHC IIdelta; MHC class IIdelta; MHC-IIdelta; MHCII-; MHCIIdelta; | ||
| Mutation Made By | Christophe Benoist, Joslin Diabetes Center | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | H1 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| Molecular Note | A 78.8 kb deletion disrupting Class II MHC genes was induced in 129S2/SvPas-derived H1 embryonic stem (ES) cells via Cre recombination. A hygro-resistance cassette was inserted at the deletion site. The deletion spans from the second exon of the H2-Ab1 gene the third exon of the H2-Ea gene. The H2-Aa, H2-Eb1 and H2-Eb2 genes are completely deleted. [MGI Ref ID J:57484] | ||
Genotyping
Genotyping Information
Genotyping Protocols
H2dlAb1-Ea, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Madsen L; Labrecque N; Engberg J; Dierich A; Svejgaard A; Benoist C; Mathis D; Fugger L. 1999. Mice lacking all conventional MHC class II genes. Proc Natl Acad Sci U S A 96(18):10338-43. [PubMed: 10468609] [MGI Ref ID J:57484]
Additional References
H2dlAb1-Ea relatedAiba Y; Kometani K; Hamadate M; Moriyama S; Sakaue-Sawano A; Tomura M; Luche H; Fehling HJ; Casellas R; Kanagawa O; Miyawaki A; Kurosaki T. 2010. Preferential localization of IgG memory B cells adjacent to contracted germinal centers. Proc Natl Acad Sci U S A 107(27):12192-7. [PubMed: 20547847] [MGI Ref ID J:162089]
Alli R; Nguyen P; Boyd K; Sundberg JP; Geiger TL. 2012. A mouse model of clonal CD8+ T lymphocyte-mediated alopecia areata progressing to alopecia universalis. J Immunol 188(1):477-86. [PubMed: 22116824] [MGI Ref ID J:180590]
Aviszus K; Macleod MK; Kirchenbaum GA; Detanico TO; Heiser RA; St Clair JB; Guo W; Wysocki LJ. 2012. Antigen-specific suppression of humoral immunity by anergic Ars/A1 B cells. J Immunol 189(9):4275-83. [PubMed: 23008448] [MGI Ref ID J:190617]
Barrett NA; Rahman OM; Fernandez JM; Parsons MW; Xing W; Austen KF; Kanaoka Y. 2011. Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes. J Exp Med 208(3):593-604. [PubMed: 21357742] [MGI Ref ID J:176841]
Bienvenu B; Martin B; Auffray C; Cordier C; Becourt C; Lucas B. 2005. Peripheral CD8+CD25+ T lymphocytes from MHC class II-deficient mice exhibit regulatory activity. J Immunol 175(1):246-53. [PubMed: 15972655] [MGI Ref ID J:100582]
Binder CJ; Hartvigsen K; Chang MK; Miller M; Broide D; Palinski W; Curtiss LK; Corr M; Witztum JL. 2004. IL-5 links adaptive and natural immunity specific for epitopes of oxidized LDL and protects from atherosclerosis. J Clin Invest 114(3):427-37. [PubMed: 15286809] [MGI Ref ID J:118092]
Bitsaktsis C; Nandi B; Racine R; MacNamara KC; Winslow G. 2007. T-Cell-independent humoral immunity is sufficient for protection against fatal intracellular ehrlichia infection. Infect Immun 75(10):4933-41. [PubMed: 17664264] [MGI Ref ID J:125283]
Blache C; Adriouch S; Calbo S; Drouot L; Dulauroy S; Arnoult C; Le Corre S; Six A; Seman M; Boyer O. 2009. Cutting edge: CD4-independent development of functional FoxP3+ regulatory t cells. J Immunol 183(7):4182-6. [PubMed: 19767568] [MGI Ref ID J:152754]
Bold TD; Ernst JD. 2012. CD4+ T cell-dependent IFN-gamma production by CD8+ effector T cells in Mycobacterium tuberculosis infection. J Immunol 189(5):2530-6. [PubMed: 22837486] [MGI Ref ID J:189869]
Bosselut R; Feigenbaum L; Sharrow SO; Singer A. 2001. Strength of signaling by CD4 and CD8 coreceptor tails determines the number but not the lineage direction of positively selected thymocytes. Immunity 14(4):483-94. [PubMed: 11336693] [MGI Ref ID J:132432]
Cheng S; Smart M; Hanson J; David CS. 2003. Characterization of HLA DR2 and DQ8 transgenic mouse with a new engineered mouse class II deletion, which lacks all endogenous class II genes. J Autoimmun 21(3):195-9. [PubMed: 14599844] [MGI Ref ID J:86434]
Choi EY; Jung KC; Park HJ; Chung DH; Song JS; Yang SD; Simpson E; Park SH. 2005. Thymocyte-thymocyte interaction for efficient positive selection and maturation of CD4 T cells. Immunity 23(4):387-96. [PubMed: 16226504] [MGI Ref ID J:113276]
Choi YS; Kageyama R; Eto D; Escobar TC; Johnston RJ; Monticelli L; Lao C; Crotty S. 2011. ICOS Receptor Instructs T Follicular Helper Cell versus Effector Cell Differentiation via Induction of the Transcriptional Repressor Bcl6. Immunity 34(6):932-46. [PubMed: 21636296] [MGI Ref ID J:174012]
Conlon TM; Cole JL; Motallebzadeh R; Harper I; Callaghan CJ; Bolton EM; Bradley JA; Saeb-Parsy K; Pettigrew GJ. 2012. Unlinked memory helper responses promote long-lasting humoral alloimmunity. J Immunol 189(12):5703-12. [PubMed: 23162131] [MGI Ref ID J:190849]
Conlon TM; Saeb-Parsy K; Cole JL; Motallebzadeh R; Qureshi MS; Rehakova S; Negus MC; Callaghan CJ; Bolton EM; Bradley JA; Pettigrew GJ. 2012. Germinal center alloantibody responses are mediated exclusively by indirect-pathway CD4 T follicular helper cells. J Immunol 188(6):2643-52. [PubMed: 22323543] [MGI Ref ID J:181855]
Deenick EK; Chan A; Ma CS; Gatto D; Schwartzberg PL; Brink R; Tangye SG. 2010. Follicular helper T cell differentiation requires continuous antigen presentation that is independent of unique B cell signaling. Immunity 33(2):241-53. [PubMed: 20691615] [MGI Ref ID J:163920]
Do JS; Valujskikh A; Vignali DA; Fairchild RL; Min B. 2012. Unexpected role for MHC II-peptide complexes in shaping CD8 T-cell expansion and differentiation in vivo. Proc Natl Acad Sci U S A 109(31):12698-703. [PubMed: 22802622] [MGI Ref ID J:188517]
Do JS; Visperas A; Oh K; Stohlman SA; Min B. 2012. Memory CD4 T cells induce selective expression of IL-27 in CD8+ dendritic cells and regulate homeostatic naive T cell proliferation. J Immunol 188(1):230-7. [PubMed: 22116827] [MGI Ref ID J:180589]
Feuillet V; Lucas B; Di Santo JP; Bismuth G; Trautmann A. 2005. Multiple survival signals are delivered by dendritic cells to naive CD4+ T cells. Eur J Immunol 35(9):2563-72. [PubMed: 16078277] [MGI Ref ID J:113487]
Freeman ML; Burkum CE; Woodland DL; Sun R; Wu TT; Blackman MA. 2012. Importance of antibody in virus infection and vaccine-mediated protection by a latency-deficient recombinant murine gamma-herpesvirus-68. J Immunol 188(3):1049-56. [PubMed: 22198955] [MGI Ref ID J:180759]
Friese MA; Jakobsen KB; Friis L; Etzensperger R; Craner MJ; McMahon RM; Jensen LT; Huygelen V; Jones EY; Bell JI; Fugger L. 2008. Opposing effects of HLA class I molecules in tuning autoreactive CD8+ T cells in multiple sclerosis. Nat Med 14(11):1227-35. [PubMed: 18953350] [MGI Ref ID J:144083]
Getahun A; Smith MJ; Kogut I; van Dyk LF; Cambier JC. 2012. Retention of anergy and inhibition of antibody responses during acute gamma herpesvirus 68 infection. J Immunol 189(6):2965-74. [PubMed: 22904300] [MGI Ref ID J:189918]
Grover HS; Blanchard N; Gonzalez F; Chan S; Robey EA; Shastri N. 2012. The Toxoplasma gondii Peptide AS15 Elicits CD4 T Cells That Can Control Parasite Burden. Infect Immun 80(9):3279-88. [PubMed: 22778097] [MGI Ref ID J:187170]
Guimond M; Veenstra RG; Grindler DJ; Zhang H; Cui Y; Murphy RD; Kim SY; Na R; Hennighausen L; Kurtulus S; Erman B; Matzinger P; Merchant MS; Mackall CL. 2009. Interleukin 7 signaling in dendritic cells regulates the homeostatic proliferation and niche size of CD4+ T cells. Nat Immunol 10(2):149-57. [PubMed: 19136960] [MGI Ref ID J:144504]
Henri S; Poulin LF; Tamoutounour S; Ardouin L; Guilliams M; de Bovis B; Devilard E; Viret C; Azukizawa H; Kissenpfennig A; Malissen B. 2010. CD207+ CD103+ dermal dendritic cells cross-present keratinocyte-derived antigens irrespective of the presence of Langerhans cells. J Exp Med 207(1):189-206, S1-6. [PubMed: 20038600] [MGI Ref ID J:156820]
Houston EG Jr; Fink PJ. 2009. MHC drives TCR repertoire shaping, but not maturation, in recent thymic emigrants. J Immunol 183(11):7244-9. [PubMed: 19915060] [MGI Ref ID J:157387]
Iijima N; Mattei LM; Iwasaki A. 2011. Recruited inflammatory monocytes stimulate antiviral Th1 immunity in infected tissue. Proc Natl Acad Sci U S A 108(1):284-9. [PubMed: 21173243] [MGI Ref ID J:169008]
Ismail N; Crossley EC; Stevenson HL; Walker DH. 2007. Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in ehrlichia-induced immunopathology in murine ehrlichiosis. Infect Immun 75(9):4608-20. [PubMed: 17562770] [MGI Ref ID J:123934]
Jacobsen EA; Zellner KR; Colbert D; Lee NA; Lee JJ. 2011. Eosinophils regulate dendritic cells and Th2 pulmonary immune responses following allergen provocation. J Immunol 187(11):6059-68. [PubMed: 22048766] [MGI Ref ID J:179701]
Kang TW; Yevsa T; Woller N; Hoenicke L; Wuestefeld T; Dauch D; Hohmeyer A; Gereke M; Rudalska R; Potapova A; Iken M; Vucur M; Weiss S; Heikenwalder M; Khan S; Gil J; Bruder D; Manns M; Schirmacher P; Tacke F; Ott M; Luedde T; Longerich T; Kubicka S; Zender L. 2011. Senescence surveillance of pre-malignant hepatocytes limits liver cancer development. Nature 479(7374):547-51. [PubMed: 22080947] [MGI Ref ID J:179823]
Kastenmuller W; Gasteiger G; Subramanian N; Sparwasser T; Busch DH; Belkaid Y; Drexler I; Germain RN. 2011. Regulatory T cells selectively control CD8+ T cell effector pool size via IL-2 restriction. J Immunol 187(6):3186-97. [PubMed: 21849683] [MGI Ref ID J:179230]
Krebs P; Barnes MJ; Lampe K; Whitley K; Bahjat KS; Beutler B; Janssen E; Hoebe K. 2009. NK cell-mediated killing of target cells triggers robust antigen-specific T cell-mediated and humoral responses. Blood 113(26):6593-602. [PubMed: 19406986] [MGI Ref ID J:150150]
Kupfer TM; Crawford ML; Pham K; Gill RG. 2005. MHC-mismatched islet allografts are vulnerable to autoimmune recognition in vivo. J Immunol 175(4):2309-16. [PubMed: 16081800] [MGI Ref ID J:107508]
Larena M; Regner M; Lee E; Lobigs M. 2011. Pivotal role of antibody and subsidiary contribution of CD8+ T cells to recovery from infection in a murine model of Japanese encephalitis. J Virol :. [PubMed: 21450826] [MGI Ref ID J:171204]
Le Campion A; Gagnerault MC; Auffray C; Becourt C; Poitrasson-Riviere M; Lallemand E; Bienvenu B; Martin B; Lepault F; Lucas B. 2009. Lymphopenia-induced spontaneous T-cell proliferation as a cofactor for autoimmune disease development. Blood 114(9):1784-93. [PubMed: 19561321] [MGI Ref ID J:152255]
Le Campion A; Pommier A; Delpoux A; Stouvenel L; Auffray C; Martin B; Lucas B. 2012. IL-2 and IL-7 determine the homeostatic balance between the regulatory and conventional CD4+ T cell compartments during peripheral T cell reconstitution. J Immunol 189(7):3339-46. [PubMed: 22933631] [MGI Ref ID J:190347]
Lee YJ; Jeon YK; Kang BH; Chung DH; Park CG; Shin HY; Jung KC; Park SH. 2010. Generation of PLZF+ CD4+ T cells via MHC class II-dependent thymocyte-thymocyte interaction is a physiological process in humans. J Exp Med 207(1):237-46, S1-7. [PubMed: 20038602] [MGI Ref ID J:156545]
Lemessurier K; Hacker H; Tuomanen E; Redecke V. 2010. Inhibition of T Cells Provides Protection against Early Invasive Pneumococcal Disease. Infect Immun 78(12):5287-94. [PubMed: 20855509] [MGI Ref ID J:165973]
Liu X; Zhan Z; Li D; Xu L; Ma F; Zhang P; Yao H; Cao X. 2011. Intracellular MHC class II molecules promote TLR-triggered innate immune responses by maintaining activation of the kinase Btk. Nat Immunol 12(5):416-24. [PubMed: 21441935] [MGI Ref ID J:171920]
Logunova NN; Viret C; Pobezinsky LA; Miller SA; Kazansky DB; Sundberg JP; Chervonsky AV. 2005. Restricted MHC-peptide repertoire predisposes to autoimmunity. J Exp Med 202(1):73-84. [PubMed: 15998789] [MGI Ref ID J:100625]
London A; Itskovich E; Benhar I; Kalchenko V; Mack M; Jung S; Schwartz M. 2011. Neuroprotection and progenitor cell renewal in the injured adult murine retina requires healing monocyte-derived macrophages. J Exp Med 208(1):23-39. [PubMed: 21220455] [MGI Ref ID J:176855]
Maehr R; Hang HC; Mintern JD; Kim YM; Cuvillier A; Nishimura M; Yamada K; Shirahama-Noda K; Hara-Nishimura I; Ploegh HL. 2005. Asparagine endopeptidase is not essential for class II MHC antigen presentation but is required for processing of cathepsin L in mice. J Immunol 174(11):7066-74. [PubMed: 15905550] [MGI Ref ID J:99005]
Maehr R; Kraus M; Ploegh HL. 2004. Mice deficient in invariant-chain and MHC class II exhibit a normal mature B2 cell compartment. Eur J Immunol 34(8):2230-6. [PubMed: 15259020] [MGI Ref ID J:91771]
Majewski M; Bose TO; Sille FC; Pollington AM; Fiebiger E; Boes M. 2007. Protein kinase C delta stimulates antigen presentation by Class II MHC in murine dendritic cells. Int Immunol 19(6):719-32. [PubMed: 17446207] [MGI Ref ID J:122307]
Mangalam A; Rodriguez M; David C. 2006. Role of MHC class II expressing CD4+ T cells in proteolipid protein91-110-induced EAE in HLA-DR3 transgenic mice. Eur J Immunol 36(12):3356-70. [PubMed: 17125142] [MGI Ref ID J:117090]
Martin B; Becourt C; Bienvenu B; Lucas B. 2006. Self-recognition is crucial for maintaining the peripheral CD4+ T-cell pool in a nonlymphopenic environment. Blood 108(1):270-7. [PubMed: 16527889] [MGI Ref ID J:135378]
Martin B; Bourgeois C; Dautigny N; Lucas B. 2003. On the role of MHC class II molecules in the survival and lymphopenia-induced proliferation of peripheral CD4+ T cells. Proc Natl Acad Sci U S A 100(10):6021-6. [PubMed: 12719530] [MGI Ref ID J:126900]
Meyer EH; Goya S; Akbari O; Berry GJ; Savage PB; Kronenberg M; Nakayama T; DeKruyff RH; Umetsu DT. 2006. Glycolipid activation of invariant T cell receptor+ NK T cells is sufficient to induce airway hyperreactivity independent of conventional CD4+ T cells. Proc Natl Acad Sci U S A 103(8):2782-7. [PubMed: 16478801] [MGI Ref ID J:107313]
Mingueneau M; Roncagalli R; Gregoire C; Kissenpfennig A; Miazek A; Archambaud C; Wang Y; Perrin P; Bertosio E; Sansoni A; Richelme S; Locksley RM; Aguado E; Malissen M; Malissen B. 2009. Loss of the LAT adaptor converts antigen-responsive T cells into pathogenic effectors that function independently of the T cell receptor. Immunity 31(2):197-208. [PubMed: 19682930] [MGI Ref ID J:151840]
Mingueneau M; Sansoni A; Gregoire C; Roncagalli R; Aguado E; Weiss A; Malissen M; Malissen B. 2008. The proline-rich sequence of CD3epsilon controls T cell antigen receptor expression on and signaling potency in preselection CD4+CD8+ thymocytes. Nat Immunol 9(5):522-32. [PubMed: 18408722] [MGI Ref ID J:134506]
Montecalvo A; Shufesky WJ; Stolz DB; Sullivan MG; Wang Z; Divito SJ; Papworth GD; Watkins SC; Robbins PD; Larregina AT; Morelli AE. 2008. Exosomes As a Short-Range Mechanism to Spread Alloantigen between Dendritic Cells during T Cell Allorecognition. J Immunol 180(5):3081-90. [PubMed: 18292531] [MGI Ref ID J:131530]
Muller AJ; Filipe-Santos O; Eberl G; Aebischer T; Spath GF; Bousso P. 2012. CD4+ T cells rely on a cytokine gradient to control intracellular pathogens beyond sites of antigen presentation. Immunity 37(1):147-57. [PubMed: 22727490] [MGI Ref ID J:187413]
Murthy AK; Cong Y; Murphey C; Guentzel MN; Forsthuber TG; Zhong G; Arulanandam BP. 2006. Chlamydial protease-like activity factor induces protective immunity against genital chlamydial infection in transgenic mice that express the human HLA-DR4 allele. Infect Immun 74(12):6722-9. [PubMed: 17015458] [MGI Ref ID J:116949]
Nakamura T; Sonoda KH; Faunce DE; Gumperz J; Yamamura T; Miyake S; Stein-Streilein J. 2003. CD4+ NKT cells, but not conventional CD4+ T cells, are required to generate efferent CD8+ T regulatory cells following antigen inoculation in an immune-privileged site. J Immunol 171(3):1266-71. [PubMed: 12874214] [MGI Ref ID J:120214]
Northrop JK; Thomas RM; Wells AD; Shen H. 2006. Epigenetic remodeling of the IL-2 and IFN-gamma loci in memory CD8 T cells is influenced by CD4 T cells. J Immunol 177(2):1062-9. [PubMed: 16818762] [MGI Ref ID J:134945]
Ohmura-Hoshino M; Matsuki Y; Mito-Yoshida M; Goto E; Aoki-Kawasumi M; Nakayama M; Ohara O; Ishido S. 2009. Cutting edge: requirement of MARCH-I-mediated MHC II ubiquitination for the maintenance of conventional dendritic cells. J Immunol 183(11):6893-7. [PubMed: 19917682] [MGI Ref ID J:157516]
Park JH; Adoro S; Guinter T; Erman B; Alag AS; Catalfamo M; Kimura MY; Cui Y; Lucas PJ; Gress RE; Kubo M; Hennighausen L; Feigenbaum L; Singer A. 2010. Signaling by intrathymic cytokines, not T cell antigen receptors, specifies CD8 lineage choice and promotes the differentiation of cytotoxic-lineage T cells. Nat Immunol 11(3):257-64. [PubMed: 20118929] [MGI Ref ID J:158504]
Poitrasson-Riviere M; Bienvenu B; Le Campion A; Becourt C; Martin B; Lucas B. 2008. Regulatory CD4+ T cells are crucial for preventing CD8+ T cell-mediated autoimmunity. J Immunol 180(11):7294-304. [PubMed: 18490729] [MGI Ref ID J:136309]
Purton JF; Tan JT; Rubinstein MP; Kim DM; Sprent J; Surh CD. 2007. Antiviral CD4+ memory T cells are IL-15 dependent. J Exp Med 204(4):951-61. [PubMed: 17420265] [MGI Ref ID J:125743]
Qian Z; Latham KA; Whittington KB; Miller DC; Brand DD; Rosloniec EF. 2010. An autoantigen-specific, highly restricted T cell repertoire infiltrates the arthritic joints of mice in an HLA-DR1 humanized mouse model of autoimmune arthritis. J Immunol 185(1):110-8. [PubMed: 20511555] [MGI Ref ID J:161436]
Quezada SA; Simpson TR; Peggs KS; Merghoub T; Vider J; Fan X; Blasberg R; Yagita H; Muranski P; Antony PA; Restifo NP; Allison JP. 2010. Tumor-reactive CD4(+) T cells develop cytotoxic activity and eradicate large established melanoma after transfer into lymphopenic hosts. J Exp Med 207(3):637-50. [PubMed: 20156971] [MGI Ref ID J:158560]
Rafei M; Hardy MP; Williams P; Vanegas JR; Forner KA; Dulude G; Labrecque N; Galipeau J; Perreault C. 2011. Development and function of innate polyclonal TCRalphabeta+ CD8+ thymocytes. J Immunol 187(6):3133-44. [PubMed: 21844388] [MGI Ref ID J:179243]
Rajagopalan G; Polich G; Sen MM; Singh M; Epstein BE; Lytle AK; Rouse MS; Patel R; David CS. 2008. Evaluating the role of HLA-DQ polymorphisms on immune response to bacterial superantigens using transgenic mice. Tissue Antigens 71(2):135-45. [PubMed: 18086265] [MGI Ref ID J:148052]
Riddle DS; Miller PJ; Vincent BG; Kepler TB; Maile R; Frelinger JA; Collins EJ. 2008. Rescue of cytotoxic function in the CD8alpha knockout mouse by removal of MHC class II. Eur J Immunol 38(6):1511-21. [PubMed: 18465769] [MGI Ref ID J:136196]
Roman E; Shino H; Qin FX; Liu YJ. 2010. Cutting edge: Hematopoietic-derived APCs select regulatory T cells in thymus. J Immunol 185(7):3819-23. [PubMed: 20802149] [MGI Ref ID J:164214]
Salek-Ardakani S; Arens R; Flynn R; Sette A; Schoenberger SP; Croft M. 2009. Preferential use of B7.2 and not B7.1 in priming of vaccinia virus-specific CD8 T cells. J Immunol 182(5):2909-18. [PubMed: 19234186] [MGI Ref ID J:146248]
Sanapala S; Yu JJ; Murthy AK; Li W; Guentzel MN; Chambers JP; Klose KE; Arulanandam BP. 2012. Perforin- and granzyme-mediated cytotoxic effector functions are essential for protection against Francisella tularensis following vaccination by the defined F. tularensis subsp. novicida DeltafopC vaccine strain. Infect Immun 80(6):2177-85. [PubMed: 22493083] [MGI Ref ID J:186522]
Song A; Song J; Tang X; Croft M. 2007. Cooperation between CD4 and CD8 T cells for anti-tumor activity is enhanced by OX40 signals. Eur J Immunol 37(5):1224-32. [PubMed: 17429847] [MGI Ref ID J:123581]
Stephens GL; Andersson J; Shevach EM. 2007. Distinct subsets of FoxP3+ regulatory T cells participate in the control of immune responses. J Immunol 178(11):6901-11. [PubMed: 17513739] [MGI Ref ID J:147842]
Tamoutounour S; Henri S; Lelouard H; de Bovis B; de Haar C; van der Woude CJ; Woltman AM; Reyal Y; Bonnet D; Sichien D; Bain CC; Mowat AM; Reis E Sousa C; Poulin LF; Malissen B; Guilliams M. 2012. CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis. Eur J Immunol 42(12):3150-66. [PubMed: 22936024] [MGI Ref ID J:190343]
Taneja V; Behrens M; Basal E; Sparks J; Griffiths MM; Luthra H; David CS. 2008. Delineating the role of the HLA-DR4 'shared epitope' in susceptibility versus resistance to develop arthritis. J Immunol 181(4):2869-77. [PubMed: 18684978] [MGI Ref ID J:140171]
Taneja V; Behrens M; Mangalam A; Griffiths MM; Luthra HS; David CS. 2007. New humanized HLA-DR4-transgenic mice that mimic the sex bias of rheumatoid arthritis. Arthritis Rheum 56(1):69-78. [PubMed: 17195209] [MGI Ref ID J:134137]
Tikhonova AN; Van Laethem F; Hanada K; Lu J; Pobezinsky LA; Hong C; Guinter TI; Jeurling SK; Bernhardt G; Park JH; Yang JC; Sun PD; Singer A. 2012. alphabeta T Cell Receptors that Do Not Undergo Major Histocompatibility Complex-Specific Thymic Selection Possess Antibody-like Recognition Specificities. Immunity 36(1):79-91. [PubMed: 22209676] [MGI Ref ID J:180750]
Tzelepis F; Persechini PM; Rodrigues MM. 2007. Modulation of CD4+ T cell-dependent specific cytotoxic CD8+ T cells differentiation and proliferation by the timing of increase in the pathogen load. PLoS ONE 2(4):e393. [PubMed: 17460760] [MGI Ref ID J:129273]
Urbieta M; Barao I; Jones M; Jurecic R; Panoskaltsis-Mortari A; Blazar BR; Murphy WJ; Levy RB. 2010. Hematopoietic progenitor cell regulation by CD4+CD25+ T cells. Blood 115(23):4934-43. [PubMed: 20200356] [MGI Ref ID J:161563]
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Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry This strain originated and is maintained on a B6,129S background. The investigator maintains the strain by mating homozygotes. Reproduction is fair. The strain is immunodeficient and sensitive to poor microbiological conditions. Pneumocystis can be a problem. Expected coat color:Black, White Bellied Agouti. Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.