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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;129S-H2dlAb1-Ea/J (Changed: 20-SEP-07 ) Type Deletion; Additional information on Mice with Chromosomal Aberrations. Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation F24 (08-JAN-08) Donating Investigator Christophe Benoist, Joslin Diabetes Center Appearance
white-bellied agouti
Related Genotype: Aw/?
black
Related Genotype: a/aDescription
Mice that are homozygous null for MHC class II genes H2-Ab1, H2-Aa, H2-Eb1, H2-Eb2, H2-Ea are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. MHC class II gene products (mRNA or protein) are not detected. A dramatic decrease is observed in the number of CD4 positive T cells in thymus, spleen and lymph nodes. This strain should serve as a suitable recipient of xenogenic Class II MHC transgenes allowing the engineering of mouse models of human MHC Class II-associated diseases.Development
This strain was developed in the laboratory of Dr. Christophe Benoist at IGBMC, Institut de Genetique et de Biologie Moleculaire et Cellulaire, Strasbourg. A 78.8 kb deletion disrupting Class II MHC genes was induced in 129S2/SvPas-derived H1 embryonic stem (ES) cells via Cre recombination. A hygro-resistance cassette was inserted at the deletion site. The deletion spans from the second exon of the H2-Ab1 gene the third exon of the H2-Ea gene. The H2-Aa, H2-Eb1 and H2-Eb2 genes are completely deleted. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were bred to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
Related Strains
Deletion
005535 B6.129S7-Del(11Cops3-Zfp179)1Jrl/J 005654 B6C3-Del(16Cbr1-ORF9)1Rhr/J 002802 C3.BLiA Pde6b+-Krd/J 004406 C3HeB/FeJ-Pou3f4del-J/J 002142 STOCK 11R30m/J 004711 STOCK Ednrbs-52Pub View Deletion (6 strains)
003584 B6.129S2-H2dlAb1-Ea/J
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
H2dlAb1-Ea/H2dlAb1-Ea
B6.129S2-H2dlAb1-Ea/J
- immune system phenotype
- increased susceptibility to bacterial infection (MGI Ref ID J:123934)
- mice are more susceptible to infection with a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE) than wild-type mice
- following infection with either a low or high dose of IOE, mice succumb to the infection at 11 to 15 days and 7 to 9 days, respectively, compared to wild-type mice, which succumb at 14 to 17 days and 8 to 12 days, respectively
- mice have higher burdens of Ehrlichia bacteria in all organs following infection than do wild-type mice
- life span-post-weaning/aging
- abnormal induced morbidity/mortality (MGI Ref ID J:123934)
- following infection with either a low or high dose of IOE, mice succumb to the infection at 11 to 15 days and 7 to 9 days, respectively, compared to wild-type mice, which succumb at 14 to 17 days and 8 to 12 days, respectively
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency (MHC class II deficient)
Lymphoid Tissue Defects
Internal/Organ Research
Lymphoid Tissue Defects
Research Tools
Immunology and Inflammation Research (MHC class II defects)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | H2dlAb1-Ea | ||
|---|---|---|---|
| Allele Name | targeted deletion, H2 complex | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) |
IIdelta;
MHC II KO;
MHC II<->;
MHC II<0>;
MHC II | ||
| Mutation Made By | Christophe Benoist, Joslin Diabetes Center | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | H1 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| Molecular Note | A 78.8 kb deletion disrupting Class II MHC genes was induced in 129S2/SvPas-derived H1 embryonic stem (ES) cells via Cre recombination. A hygro-resistance cassette was inserted at the deletion site. The deletion spans from the second exon of the H2-Ab1 gene the third exon of the H2-Ea gene. The H2-Aa, H2-Eb1 and H2-Eb2 genes are completely deleted. J: [MGI Ref ID J:57484] | ||
Genotyping
Genotyping Information
Genotyping Protocols
H2dlAb1-Ea, HRM, vers. 1
H2dlAb1-Ea, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Madsen L; Labrecque N; Engberg J; Dierich A; Svejgaard A; Benoist C; Mathis D; Fugger L. 1999. Mice lacking all conventional MHC class II genes. Proc Natl Acad Sci U S A 96(18):10338-43. [PubMed: 10468609] [MGI Ref ID J:57484]
Additional References
H2dlAb1-Ea relatedBienvenu B; Martin B; Auffray C; Cordier C; Becourt C; Lucas B. 2005. Peripheral CD8+CD25+ T lymphocytes from MHC class II-deficient mice exhibit regulatory activity. J Immunol 175(1):246-53. [PubMed: 15972655] [MGI Ref ID J:100582]
Binder CJ; Hartvigsen K; Chang MK; Miller M; Broide D; Palinski W; Curtiss LK; Corr M; Witztum JL. 2004. IL-5 links adaptive and natural immunity specific for epitopes of oxidized LDL and protects from atherosclerosis. J Clin Invest 114(3):427-37. [PubMed: 15286809] [MGI Ref ID J:118092]
Bitsaktsis C; Nandi B; Racine R; MacNamara KC; Winslow G. 2007. T-Cell-independent humoral immunity is sufficient for protection against fatal intracellular ehrlichia infection. Infect Immun 75(10):4933-41. [PubMed: 17664264] [MGI Ref ID J:125283]
Bosselut R; Feigenbaum L; Sharrow SO; Singer A. 2001. Strength of signaling by CD4 and CD8 coreceptor tails determines the number but not the lineage direction of positively selected thymocytes. Immunity 14(4):483-94. [PubMed: 11336693] [MGI Ref ID J:132432]
Cheng S; Smart M; Hanson J; David CS. 2003. Characterization of HLA DR2 and DQ8 transgenic mouse with a new engineered mouse class II deletion, which lacks all endogenous class II genes. J Autoimmun 21(3):195-9. [PubMed: 14599844] [MGI Ref ID J:86434]
Choi EY; Jung KC; Park HJ; Chung DH; Song JS; Yang SD; Simpson E; Park SH. 2005. Thymocyte-thymocyte interaction for efficient positive selection and maturation of CD4 T cells. Immunity 23(4):387-96. [PubMed: 16226504] [MGI Ref ID J:113276]
Feuillet V; Lucas B; Di Santo JP; Bismuth G; Trautmann A. 2005. Multiple survival signals are delivered by dendritic cells to naive CD4+ T cells. Eur J Immunol 35(9):2563-72. [PubMed: 16078277] [MGI Ref ID J:113487]
Ismail N; Crossley EC; Stevenson HL; Walker DH. 2007. Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in ehrlichia-induced immunopathology in murine ehrlichiosis. Infect Immun 75(9):4608-20. [PubMed: 17562770] [MGI Ref ID J:123934]
Kupfer TM; Crawford ML; Pham K; Gill RG. 2005. MHC-mismatched islet allografts are vulnerable to autoimmune recognition in vivo. J Immunol 175(4):2309-16. [PubMed: 16081800] [MGI Ref ID J:107508]
Logunova NN; Viret C; Pobezinsky LA; Miller SA; Kazansky DB; Sundberg JP; Chervonsky AV. 2005. Restricted MHC-peptide repertoire predisposes to autoimmunity. J Exp Med 202(1):73-84. [PubMed: 15998789] [MGI Ref ID J:100625]
Maehr R; Hang HC; Mintern JD; Kim YM; Cuvillier A; Nishimura M; Yamada K; Shirahama-Noda K; Hara-Nishimura I; Ploegh HL. 2005. Asparagine endopeptidase is not essential for class II MHC antigen presentation but is required for processing of cathepsin L in mice. J Immunol 174(11):7066-74. [PubMed: 15905550] [MGI Ref ID J:99005]
Maehr R; Kraus M; Ploegh HL. 2004. Mice deficient in invariant-chain and MHC class II exhibit a normal mature B2 cell compartment. Eur J Immunol 34(8):2230-6. [PubMed: 15259020] [MGI Ref ID J:91771]
Majewski M; Bose TO; Sille FC; Pollington AM; Fiebiger E; Boes M. 2007. Protein kinase C delta stimulates antigen presentation by Class II MHC in murine dendritic cells. Int Immunol 19(6):719-32. [PubMed: 17446207] [MGI Ref ID J:122307]
Mangalam A; Rodriguez M; David C. 2006. Role of MHC class II expressing CD4+ T cells in proteolipid protein91-110-induced EAE in HLA-DR3 transgenic mice. Eur J Immunol 36(12):3356-70. [PubMed: 17125142] [MGI Ref ID J:117090]
Martin B; Becourt C; Bienvenu B; Lucas B. 2006. Self-recognition is crucial for maintaining the peripheral CD4+ T-cell pool in a nonlymphopenic environment. Blood 108(1):270-7. [PubMed: 16527889] [MGI Ref ID J:135378]
Martin B; Bourgeois C; Dautigny N; Lucas B. 2003. On the role of MHC class II molecules in the survival and lymphopenia-induced proliferation of peripheral CD4+ T cells. Proc Natl Acad Sci U S A 100(10):6021-6. [PubMed: 12719530] [MGI Ref ID J:126900]
Meyer EH; Goya S; Akbari O; Berry GJ; Savage PB; Kronenberg M; Nakayama T; DeKruyff RH; Umetsu DT. 2006. Glycolipid activation of invariant T cell receptor+ NK T cells is sufficient to induce airway hyperreactivity independent of conventional CD4+ T cells. Proc Natl Acad Sci U S A 103(8):2782-7. [PubMed: 16478801] [MGI Ref ID J:107313]
Mingueneau M; Sansoni A; Gregoire C; Roncagalli R; Aguado E; Weiss A; Malissen M; Malissen B. 2008. The proline-rich sequence of CD3epsilon controls T cell antigen receptor expression on and signaling potency in preselection CD4+CD8+ thymocytes. Nat Immunol 9(5):522-32. [PubMed: 18408722] [MGI Ref ID J:134506]
Montecalvo A; Shufesky WJ; Stolz DB; Sullivan MG; Wang Z; Divito SJ; Papworth GD; Watkins SC; Robbins PD; Larregina AT; Morelli AE. 2008. Exosomes As a Short-Range Mechanism to Spread Alloantigen between Dendritic Cells during T Cell Allorecognition. J Immunol 180(5):3081-90. [PubMed: 18292531] [MGI Ref ID J:131530]
Murthy AK; Cong Y; Murphey C; Guentzel MN; Forsthuber TG; Zhong G; Arulanandam BP. 2006. Chlamydial protease-like activity factor induces protective immunity against genital chlamydial infection in transgenic mice that express the human HLA-DR4 allele. Infect Immun 74(12):6722-9. [PubMed: 17015458] [MGI Ref ID J:116949]
Nakamura T; Sonoda KH; Faunce DE; Gumperz J; Yamamura T; Miyake S; Stein-Streilein J. 2003. CD4+ NKT cells, but not conventional CD4+ T cells, are required to generate efferent CD8+ T regulatory cells following antigen inoculation in an immune-privileged site. J Immunol 171(3):1266-71. [PubMed: 12874214] [MGI Ref ID J:120214]
Northrop JK; Thomas RM; Wells AD; Shen H. 2006. Epigenetic remodeling of the IL-2 and IFN-gamma loci in memory CD8 T cells is influenced by CD4 T cells. J Immunol 177(2):1062-9. [PubMed: 16818762] [MGI Ref ID J:134945]
Poitrasson-Riviere M; Bienvenu B; Le Campion A; Becourt C; Martin B; Lucas B. 2008. Regulatory CD4+ T cells are crucial for preventing CD8+ T cell-mediated autoimmunity. J Immunol 180(11):7294-304. [PubMed: 18490729] [MGI Ref ID J:136309]
Purton JF; Tan JT; Rubinstein MP; Kim DM; Sprent J; Surh CD. 2007. Antiviral CD4+ memory T cells are IL-15 dependent. J Exp Med 204(4):951-61. [PubMed: 17420265] [MGI Ref ID J:125743]
Riddle DS; Miller PJ; Vincent BG; Kepler TB; Maile R; Frelinger JA; Collins EJ. 2008. Rescue of cytotoxic function in the CD8alpha knockout mouse by removal of MHC class II. Eur J Immunol 38(6):1511-21. [PubMed: 18465769] [MGI Ref ID J:136196]
Song A; Song J; Tang X; Croft M. 2007. Cooperation between CD4 and CD8 T cells for anti-tumor activity is enhanced by OX40 signals. Eur J Immunol 37(5):1224-32. [PubMed: 17429847] [MGI Ref ID J:123581]
Taneja V; Behrens M; Basal E; Sparks J; Griffiths MM; Luthra H; David CS. 2008. Delineating the role of the HLA-DR4 'shared epitope' in susceptibility versus resistance to develop arthritis. J Immunol 181(4):2869-77. [PubMed: 18684978] [MGI Ref ID J:140171]
Taneja V; Behrens M; Mangalam A; Griffiths MM; Luthra HS; David CS. 2007. New humanized HLA-DR4-transgenic mice that mimic the sex bias of rheumatoid arthritis. Arthritis Rheum 56(1):69-78. [PubMed: 17195209] [MGI Ref ID J:134137]
Tzelepis F; Persechini PM; Rodrigues MM. 2007. Modulation of CD4+ T cell-dependent specific cytotoxic CD8+ T cells differentiation and proliferation by the timing of increase in the pathogen load. PLoS ONE 2(4):e393. [PubMed: 17460760] [MGI Ref ID J:129273]
Voehringer D; Reese TA; Huang X; Shinkai K; Locksley RM. 2006. Type 2 immunity is controlled by IL-4/IL-13 expression in hematopoietic non-eosinophil cells of the innate immune system. J Exp Med 203(6):1435-46. [PubMed: 16702603] [MGI Ref ID J:124383]
Wang L; Wildt KF; Zhu J; Zhang X; Feigenbaum L; Tessarollo L; Paul WE; Fowlkes BJ; Bosselut R. 2008. Distinct functions for the transcription factors GATA-3 and ThPOK during intrathymic differentiation of CD4(+) T cells. Nat Immunol 9(10):1122-30. [PubMed: 18776904] [MGI Ref ID J:141145]
Wang Y; Kissenpfennig A; Mingueneau M; Richelme S; Perrin P; Chevrier S; Genton C; Lucas B; DiSanto JP; Acha-Orbea H; Malissen B; Malissen M. 2008. Th2 lymphoproliferative disorder of LatY136F mutant mice unfolds independently of TCR-MHC engagement and is insensitive to the action of Foxp3+ regulatory T cells. J Immunol 180(3):1565-75. [PubMed: 18209052] [MGI Ref ID J:131351]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain originated and is maintained on a B6,129S background. The investigator maintains the strain by mating homozygotes. Reproduction is fair. The strain is immunodeficient and sensitive to poor microbiological conditions. Pneumocystis can be a problem. Expected coat color:Black, White Bellied Agouti. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $107.60 Female or Male Homozygous for H2dlAb1-Ea *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $215.20 Homozygous for H2dlAb1-Ea x Homozygous for H2dlAb1-Ea
Additional Supply Details
| Supply Notes |
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|---|
| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $139.90 Female or Male Homozygous for H2dlAb1-Ea *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $279.80 Homozygous for H2dlAb1-Ea x Homozygous for H2dlAb1-Ea
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
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Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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