Strain Name: |
FVB/N-Tg(ACTB-cre)2Mrt/J |
|---|---|
Stock Number: |
003376 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
FVB/N-TgN(ACTB-Cre)2Mrt (Changed: 15-DEC-04
) |
| Genes & Alleles | ACTB; cre; Tg(ACTB-cre)2Mrt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Gail Martin, University of California San Francisco Strain Description
This Cre recombinase strain is under the human beta actin gene promoter. This strain expresses Cre recombinase in all cells of the embryo by the blastocyst stage of development. It is useful as a "deletor" mouse for virtually any genomic sequence flanked by loxP sites.
Gene & Allele Details
| Allele Symbol | Tg(ACTB-cre)2Mrt | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Gail R Martin | ||
| Common Name(s) | A-cre; Act-Cre; TgN(ACTB-cre)2Mrt; actin-cre; beta-actin-Cre; beta-actin::Cre; creactin; | ||
| Mutation Made By | Gail Martin, University of California San Francisco | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | all cells of the embryo by the blastocyst stage of development; widespread expression | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | ACTB, actin, beta, human | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a human beta-actin promoter, which is active in all embryonic cells at or before the blastocyst stage. [MGI Ref ID J:46833] | ||
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | The strain originated on and is currently on an FVB/N background. Homozygotes are viable and fertile. Expected coat color from breeding:Albino |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of ACTB
005703 B6.Cg-Tg(ACTFLPe)9205Dym/J 006055 B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J 003800 B6;SJL-Tg(ACTFLPe)9205Dym/J 005145 C57BL/6-Tg(CAG-OVA)916Jen/J 005863 C57BL/6J-Tg(ACTB-DDAH1)1Jpck/J 002981 DBA/2-Tg(xstpx-lacZ)36And/J 005438 STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J View Strains carrying other alleles of ACTB (7 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesLewandoski M; Meyers EN; Martin GR. 1997. Analysis of Fgf8 gene function in vertebrate development. Cold Spring Harb Symp Quant Biol 62:159-68. [PubMed: 9598348] [MGI Ref ID J:46833]
Price and Supply Information
| Strain Name: | FVB/N-Tg(ACTB-cre)2Mrt/J |
| Stock Number: | 003376 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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