Description

Strain Information

Former Names B6;129S2-Srb1tm1Kri    (Changed: 15-DEC-04 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x +/+ sibling         (Female x Male)   14-SEP-11 Mating System +/+ sibling x Heterozygote         (Female x Male)   07-FEB-11 Species laboratory mouse Generation F29 (30-NOV-12) Generation Definitions   Donating Investigator Dr. Monty Krieger,   Massachusetts Institute of Technology

Description
The class B, type I scavenger receptor (Srb1 or Scarb1) is a cell surface HDL receptor that can recognize the apolipoproteins on the surface of the HDL particle. It plays a key role in determining the levels of plasma lipoprotein cholesterol (primarily HDL) and the accumulation of cholesterol stores in the adrenal gland.In this strain plasma cholesterol (primarily HDL) concentrations increase by 125% in homozygotes and 31% in heterozygotes, as compared to wild type controls. Also, cholesterol levels in adrenal tissue in homozygous and heterozygous mutants decrease by 72% and 42% respectively, relative to wild type controls. The plasma concentration of Apoa-I, the major protein in HDL, is unchanged in mutant animals, relative to wild type controls.Homozygous females are infertile; homozygous males are fertile. Please note that the donating investigator reports that the number of homozygotes resulting from a cross between heterozygotes is significantly lower than what the expected Mendelian ratio would predict.

Development
The strain was made with replacement of a portion of the first coding exon (including the initiator ATG and sequences encoding a putative membrane spanning region) with a neomycin resistance gene. The strain originated on a mixed B6,129 background and is currently on the same background.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Related Strains

Strains carrying   Scarb1^tm1Kri allele

004362

B6;129-Scarb1tm1Kri Apoetm1Unc/J

View Strains carrying   Scarb1^tm1Kri     (1 strain)

Strains carrying other alleles of Scarb1

005495

C57BL/6J-Scarb1Hlb398/J

View Strains carrying other alleles of Scarb1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Scarb1^tm1Kri/Scarb1⁺

        involves: 129S2/SvPas * C57BL/6

• homeostasis/metabolism phenotype
• abnormal lipid homeostasis   (MGI Ref ID J:44187)
• • abnormal cholesterol level   (MGI Ref ID J:44187)
  • • increased circulating cholesterol level
      • plasma cholesterol concentration increased by ~31%   (MGI Ref ID J:44187)
• endocrine/exocrine gland phenotype
• abnormal adrenal gland physiology
  • cholesterol content decreased by 42%   (MGI Ref ID J:44187)

Scarb1^tm1Kri/Scarb1^tm1Kri

        involves: 129S2/SvPas * C57BL/6

• mortality/aging
• partial neonatal lethality
  • the occasional offspring produced by homozygous mothers die soon after birth   (MGI Ref ID J:105226)
• homeostasis/metabolism phenotype
• abnormal lipid homeostasis   (MGI Ref ID J:44187)
• • abnormal cholesterol level   (MGI Ref ID J:44187)
  • • abnormal circulating cholesterol level
      • plasma total cholesterol is abnormally high   (MGI Ref ID J:73138)
      • most of the cholesterol is found as abnormally large particles   (MGI Ref ID J:73138)
    • • increased circulating cholesterol level
        • plasma concentrations are 125% of control values   (MGI Ref ID J:44187)
        • concentration increase is due to formation of large particles containing apolipoprotein A-1   (MGI Ref ID J:44187)
• endocrine/exocrine gland phenotype
• abnormal adrenal gland physiology
  • cholesterol content is 72% lower than normal   (MGI Ref ID J:44187)
• reproductive system phenotype
• abnormal oocyte morphology
  • some superovulated oocytes have an abnormal morphology   (MGI Ref ID J:105226)
• female infertility
  • only occasionally will a pup be born   (MGI Ref ID J:105226)
  • inactivation of the apolipoprotein A-1 gene or administration of the cholesterol-lowering drug probucol restored fertility   (MGI Ref ID J:105226)
  • inactivation of the apolipoprotein A-1 gene or administration of the cholesterol-lowering drug probucol restored fertility   (MGI Ref ID J:73138)
• hematopoietic system phenotype
• abnormal erythrocyte morphology
  • red blood cells with cytoplasmic irregularities   (MGI Ref ID J:75090)
  • mitochondria present in about 15% of red blood cells   (MGI Ref ID J:75090)
• • anemia
    • mild   (MGI Ref ID J:75090)
    • exacerbated on a high cholesterol diet   (MGI Ref ID J:75090)
  • macrocytosis   (MGI Ref ID J:75090)
  • poikilocytosis
    • sometimes   (MGI Ref ID J:75090)
• embryogenesis phenotype
• abnormal embryonic tissue morphology
  • the majority of harvested one cell zygotes had an abnormal, nonrefractile morphology   (MGI Ref ID J:105226)
• failure of zygotic cell division
  • in vitro development of zygotes arrests by the 2 cell stage   (MGI Ref ID J:105226)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Scarb1^tm1Kri/Scarb1^tm1Kri

        B6.129S2-Scarb1^tm1Kri

• reproductive system phenotype
• female infertility
  • only occasionally is a pup born   (MGI Ref ID J:73138)

Scarb1^tm1Kri/Scarb1^tm1Kri

        involves: 129S2/SvPas

• cardiovascular system phenotype
• abnormal vascular wound healing
  • reendothelialization is delayed compared to in wild-type mice following injury to the carotid artery   (MGI Ref ID J:118063)
  • mice exhibit a 44% larger area of non-reendothelialized carotid artery following injury compared to in wild-type mice   (MGI Ref ID J:118063)
• homeostasis/metabolism phenotype
• abnormal vascular wound healing
  • reendothelialization is delayed compared to in wild-type mice following injury to the carotid artery   (MGI Ref ID J:118063)
  • mice exhibit a 44% larger area of non-reendothelialized carotid artery following injury compared to in wild-type mice   (MGI Ref ID J:118063)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Scarb1^tm1Kri related

Cardiovascular Research
Hypercholesterolemia

Metabolism Research
Lipid Metabolism

Reproductive Biology Research
Fertility Defects
      females only

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Scarb1tm1Kri
Allele Name		targeted mutation 1, Monty Krieger
Allele Type		Targeted (knock-out)
Common Name(s)		SR-BI; SR-BI KO; SR-BI-; Sr-b1-; srb1-; srbI-;
Mutation Made By		Dr. Monty Krieger,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Scarb1, scavenger receptor class B, member 1
Chromosome		5
Gene Common Name(s)		AI120173; CD36L1; CLA-1; CLA1; D5Ertd460e; DNA segment, Chr 5, ERATO Doi 460, expressed; HDL QTL 1; HDLQTL6; Hdlq1; Hlb398; SR-B1; SR-BI; SRB1; SRBI; Srb1; expressed sequence AI120173; heart, lung and blood 398; scavenger receptor class B1;
Molecular Note		Replacement of the entire coding region of the first exon and an additional 554 bases of intron 1 with a neomycin cassette. [MGI Ref ID J:44187]

Genotyping

Genotyping Information

Genotyping Protocols

Scarb1^tm1Kri, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Rigotti A; Trigatti BL; Penman M; Rayburn H; Herz J; Krieger M. 1997. A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism. Proc Natl Acad Sci U S A 94(23):12610-5. [PubMed: 9356497]  [MGI Ref ID J:44187]

Additional References

Scarb1^tm1Kri related

Adorni MP; Zimetti F; Billheimer JT; Wang N; Rader DJ; Phillips MC; Rothblat GH. 2007. The roles of different pathways in the release of cholesterol from macrophages. J Lipid Res 48(11):2453-62. [PubMed: 17761631]  [MGI Ref ID J:127358]

Altmann SW; Davis HR; Yao X; Laverty M; Compton DS; Zhu L; Crona JH; Caplen MA; Hoos LM; Tetzloff G; Priestley T; Burnett DA; Strader CD; Graziano MP. 2002. The identification of intestinal scavenger receptor class B, type I (SR-BI) by expression cloning and its role in cholesterol absorption. Biochim Biophys Acta 1580(1):77-93. [PubMed: 11923102]  [MGI Ref ID J:75435]

Annema W; Dikkers A; Freark de Boer J; Gautier T; Rensen PC; Rader DJ; Tietge UJ. 2012. ApoE promotes hepatic selective uptake but not RCT due to increased ABCA1-mediated cholesterol efflux to plasma. J Lipid Res 53(5):929-40. [PubMed: 22383685]  [MGI Ref ID J:184916]

Baranova IN; Vishnyakova TG; Bocharov AV; Leelahavanichkul A; Kurlander R; Chen Z; Souza AC; Yuen PS; Star RA; Csako G; Patterson AP; Eggerman TL. 2012. Class B scavenger receptor types I and II and CD36 mediate bacterial recognition and proinflammatory signaling induced by Escherichia coli, lipopolysaccharide, and cytosolic chaperonin 60. J Immunol 188(3):1371-80. [PubMed: 22205027]  [MGI Ref ID J:180755]

Braun A; Trigatti BL; Post MJ; Sato K; Simons M; Edelberg JM; Rosenberg RD; Schrenzel M; Krieger M. 2002. Loss of SR-BI expression leads to the early onset of occlusive atherosclerotic coronary artery disease, spontaneous myocardial infarctions, severe cardiac dysfunction, and premature death in apolipoprotein E-deficient mice. Circ Res 90(3):270-6. [PubMed: 11861414]  [MGI Ref ID J:109751]

Braun A; Zhang S; Miettinen HE; Ebrahim S; Holm TM; Vasile E; Post MJ; Yoerger DM; Picard MH; Krieger JL; Andrews NC; Simons M; Krieger M. 2003. Probucol prevents early coronary heart disease and death in the high-density lipoprotein receptor SR-BI/apolipoprotein E double knockout mouse. Proc Natl Acad Sci U S A 100(12):7283-8. [PubMed: 12771386]  [MGI Ref ID J:83785]

Bravo I; Amigo L; Cohen DE; Nervi F; Rigotti A; Francone O; Zanlungo S. 2007. Role of plasma and liver cholesterol- and lipoprotein-metabolism determinants in LpX formation in the mouse. Biochim Biophys Acta 1770(6):979-88. [PubMed: 17399905]  [MGI Ref ID J:121757]

Brodeur MR; Luangrath V; Bourret G; Falstrault L; Brissette L. 2005. Physiological importance of SR-BI in the in vivo metabolism of human HDL and LDL in male and female mice. J Lipid Res 46(4):687-96. [PubMed: 15654132]  [MGI Ref ID J:98578]

Brundert M; Heeren J; Bahar-Bayansar M; Ewert A; Moore KJ; Rinninger F. 2006. Selective uptake of HDL cholesteryl esters and cholesterol efflux from mouse peritoneal macrophages independent of SR-BI. J Lipid Res 47(11):2408-21. [PubMed: 16926440]  [MGI Ref ID J:147399]

Cai L; Ji A; de Beer FC; Tannock LR; van der Westhuyzen DR. 2008. SR-BI protects against endotoxemia in mice through its roles in glucocorticoid production and hepatic clearance. J Clin Invest 118(1):364-75. [PubMed: 18064300]  [MGI Ref ID J:130849]

Cai L; Wang Z; Meyer JM; Ji A; van der Westhuyzen DR. 2012. Macrophage SR-BI regulates LPS-induced pro-inflammatory signaling in mice and isolated macrophages. J Lipid Res 53(8):1472-81. [PubMed: 22589557]  [MGI Ref ID J:186565]

Callow MJ; Dudoit S; Gong EL; Speed TP; Rubin EM. 2000. Microarray expression profiling identifies genes with altered expression in HDL-deficient mice. Genome Res 10(12):2022-9. [PubMed: 11116096]  [MGI Ref ID J:115384]

Chang EH; Rigotti A; Huerta PT. 2009. Age-related influence of the HDL receptor SR-BI on synaptic plasticity and cognition. Neurobiol Aging 30(3):407-19. [PubMed: 17719144]  [MGI Ref ID J:145814]

Cuchel M; Lund-Katz S; de la Llera-Moya M; Millar JS; Chang D; Fuki I; Rothblat GH; Phillips MC; Rader DJ. 2010. Pathways by which reconstituted high-density lipoprotein mobilizes free cholesterol from whole body and from macrophages. Arterioscler Thromb Vasc Biol 30(3):526-32. [PubMed: 20018934]  [MGI Ref ID J:172105]

Dole VS; Matuskova J; Vasile E; Yesilaltay A; Bergmeier W; Bernimoulin M; Wagner DD; Krieger M. 2008. Thrombocytopenia and platelet abnormalities in high-density lipoprotein receptor-deficient mice. Arterioscler Thromb Vasc Biol 28(6):1111-6. [PubMed: 18436807]  [MGI Ref ID J:149018]

Dorner M; Horwitz JA; Robbins JB; Barry WT; Feng Q; Mu K; Jones CT; Schoggins JW; Catanese MT; Burton DR; Law M; Rice CM; Ploss A. 2011. A genetically humanized mouse model for hepatitis C virus infection. Nature 474(7350):208-11. [PubMed: 21654804]  [MGI Ref ID J:173453]

Drover VA; Nguyen DV; Bastie CC; Darlington YF; Abumrad NA; Pessin JE; London E; Sahoo D; Phillips MC. 2008. CD36 mediates both cellular uptake of very long chain fatty acids and their intestinal absorption in mice. J Biol Chem 283(19):13108-15. [PubMed: 18332148]  [MGI Ref ID J:137082]

Feng H; Guo L; Wang D; Gao H; Hou G; Zheng Z; Ai J; Foreman O; Daugherty A; Li XA. 2011. Deficiency of scavenger receptor BI leads to impaired lymphocyte homeostasis and autoimmune disorders in mice. Arterioscler Thromb Vasc Biol 31(11):2543-51. [PubMed: 21836069]  [MGI Ref ID J:191836]

Guo L; Song Z; Li M; Wu Q; Wang D; Feng H; Bernard P; Daugherty A; Huang B; Li XA. 2009. Scavenger Receptor BI Protects against Septic Death through Its Role in Modulating Inflammatory Response. J Biol Chem 284(30):19826-34. [PubMed: 19491399]  [MGI Ref ID J:152734]

Harder C; Lau P; Meng A; Whitman SC; McPherson R. 2007. Cholesteryl ester transfer protein (CETP) expression protects against diet induced atherosclerosis in SR-BI deficient mice. Arterioscler Thromb Vasc Biol 27(4):858-64. [PubMed: 17272756]  [MGI Ref ID J:128221]

Hildebrand RB; Lammers B; Meurs I; Korporaal SJ; De Haan W; Zhao Y; Kruijt JK; Pratico D; Schimmel AW; Holleboom AG; Hoekstra M; Kuivenhoven JA; Van Berkel TJ; Rensen PC; Van Eck M. 2010. Restoration of high-density lipoprotein levels by cholesteryl ester transfer protein expression in scavenger receptor class B type I (SR-BI) knockout mice does not normalize pathologies associated with SR-BI deficiency. Arterioscler Thromb Vasc Biol 30(7):1439-45. [PubMed: 20431066]  [MGI Ref ID J:180862]

Hoekstra M; Meurs I; Koenders M; Out R; Hildebrand RB; Kruijt JK; Van Eck M; Van Berkel TJ. 2008. Absence of HDL cholesteryl ester uptake in mice via SR-BI impairs an adequate adrenal glucocorticoid-mediated stress response to fasting. J Lipid Res 49(4):738-45. [PubMed: 18204096]  [MGI Ref ID J:133584]

Hoekstra M; Ye D; Hildebrand RB; Zhao Y; Lammers B; Stitzinger M; Kuiper J; Van Berkel TJ; Van Eck M. 2009. Scavenger receptor class B type I-mediated uptake of serum cholesterol is essential for optimal adrenal glucocorticoid production. J Lipid Res 50(6):1039-46. [PubMed: 19179307]  [MGI Ref ID J:149859]

Holm TM; Braun A; Trigatti BL; Brugnara C; Sakamoto M; Krieger M; Andrews NC. 2002. Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI. Blood 99(5):1817-24. [PubMed: 11861300]  [MGI Ref ID J:75090]

Ji A; Wroblewski JM; Cai L; de Beer MC; Webb NR; van der Westhuyzen DR. 2012. Nascent HDL formation in hepatocytes and role of ABCA1, ABCG1, and SR-BI. J Lipid Res 53(3):446-55. [PubMed: 22190590]  [MGI Ref ID J:183126]

Jimenez LM; Binelli M; Bertolin K; Pelletier RM; Murphy BD. 2010. Scavenger receptor-B1 and luteal function in mice. J Lipid Res 51(8):2362-71. [PubMed: 20404351]  [MGI Ref ID J:162944]

Karackattu SL; Picard MH; Krieger M. 2005. Lymphocytes are not required for the rapid onset of coronary heart disease in scavenger receptor class B type I/apolipoprotein E double knockout mice. Arterioscler Thromb Vasc Biol 25(4):803-8. [PubMed: 15692099]  [MGI Ref ID J:110023]

Karackattu SL; Trigatti B; Krieger M. 2006. Hepatic lipase deficiency delays atherosclerosis, myocardial infarction, and cardiac dysfunction and extends lifespan in SR-BI/apolipoprotein E double knockout mice. Arterioscler Thromb Vasc Biol 26(3):548-54. [PubMed: 16397139]  [MGI Ref ID J:127979]

Kocher O; Yesilaltay A; Shen CH; Zhang S; Daniels K; Pal R; Chen J; Krieger M. 2008. Influence of PDZK1 on lipoprotein metabolism and atherosclerosis. Biochim Biophys Acta 1782(5):310-6. [PubMed: 18342019]  [MGI Ref ID J:136729]

Korporaal SJ; Meurs I; Hauer AD; Hildebrand RB; Hoekstra M; Cate HT; Pratico D; Akkerman JW; Van Berkel TJ; Kuiper J; Van Eck M. 2011. Deletion of the high-density lipoprotein receptor scavenger receptor BI in mice modulates thrombosis susceptibility and indirectly affects platelet function by elevation of plasma free cholesterol. Arterioscler Thromb Vasc Biol 31(1):34-42. [PubMed: 21051668]  [MGI Ref ID J:184188]

Lee JY; Badeau RM; Mulya A; Boudyguina E; Gebre AK; Smith TL; Parks JS. 2007. Functional LCAT deficiency in human apolipoprotein A-I transgenic, SR-BI knockout mice. J Lipid Res 48(5):1052-61. [PubMed: 17272829]  [MGI Ref ID J:121837]

Leelahavanichkul A; Bocharov AV; Kurlander R; Baranova IN; Vishnyakova TG; Souza AC; Hu X; Doi K; Vaisman B; Amar M; Sviridov D; Chen Z; Remaley AT; Csako G; Patterson AP; Yuen PS; Star RA; Eggerman TL. 2012. Class B Scavenger Receptor Types I and II and CD36 Targeting Improves Sepsis Survival and Acute Outcomes in Mice. J Immunol 188(6):2749-58. [PubMed: 22327076]  [MGI Ref ID J:181845]

Li XA; Guo L; Asmis R; Nikolova-Karakashian M; Smart EJ. 2006. Scavenger receptor BI prevents nitric oxide-induced cytotoxicity and endotoxin-induced death. Circ Res 98(7):e60-5. [PubMed: 16574909]  [MGI Ref ID J:121399]

Li XA; Guo L; Dressman JL; Asmis R; Smart EJ. 2005. A novel ligand-independent apoptotic pathway induced by scavenger receptor class B, type I and suppressed by endothelial nitric-oxide synthase and high density lipoprotein. J Biol Chem 280(19):19087-96. [PubMed: 15749707]  [MGI Ref ID J:99934]

Lindegaard ML; Wassif CA; Vaisman B; Amar M; Wasmuth EV; Shamburek R; Nielsen LB; Remaley AT; Porter FD. 2008. Characterization of placental cholesterol transport: ABCA1 is a potential target for in utero therapy of Smith-Lemli-Opitz syndrome. Hum Mol Genet 17(23):3806-13. [PubMed: 18775956]  [MGI Ref ID J:141147]

Luangrath V; Brodeur MR; Rhainds D; Brissette L. 2008. Mouse CD36 has opposite effects on LDL and oxidized LDL metabolism in vivo. Arterioscler Thromb Vasc Biol 28(7):1290-5. [PubMed: 18436808]  [MGI Ref ID J:159812]

Ma K; Forte T; Otvos JD; Chan L. 2005. Differential additive effects of endothelial lipase and scavenger receptor-class B type I on high-density lipoprotein metabolism in knockout mouse models. Arterioscler Thromb Vasc Biol 25(1):149-54. [PubMed: 15539616]  [MGI Ref ID J:109812]

Ma Y; Ashraf MZ; Podrez EA. 2010. Scavenger receptor BI modulates platelet reactivity and thrombosis in dyslipidemia. Blood 116(11):1932-41. [PubMed: 20508162]  [MGI Ref ID J:164537]

Mardones P; Quinones V; Amigo L; Moreno M; Miquel JF; Schwarz M; Miettinen HE; Trigatti B; Krieger M; VanPatten S; Cohen DE; Rigotti A. 2001. Hepatic cholesterol and bile acid metabolism and intestinal cholesterol absorption in scavenger receptor class B type I-deficient mice. J Lipid Res 42(2):170-80. [PubMed: 11181745]  [MGI Ref ID J:68352]

Mardones P; Strobel P; Miranda S; Leighton F; Quinones V; Amigo L; Rozowski J; Krieger M; Rigotti A. 2002. Alpha-tocopherol metabolism is abnormal in scavenger receptor class B type I (SR-BI)-deficient mice. J Nutr 132(3):443-9. [PubMed: 11880569]  [MGI Ref ID J:75363]

Meurs I; Hoekstra M; van Wanrooij EJ; Hildebrand RB; Kuiper J; Kuipers F; Hardeman MR; Van Berkel TJ; Van Eck M. 2005. HDL cholesterol levels are an important factor for determining the lifespan of erythrocytes. Exp Hematol 33(11):1309-19. [PubMed: 16263415]  [MGI Ref ID J:102414]

Miettinen HE; Rayburn H; Krieger M. 2001. Abnormal lipoprotein metabolism and reversible female infertility in HDL receptor (SR-BI)-deficient mice. J Clin Invest 108(11):1717-22. [PubMed: 11733567]  [MGI Ref ID J:73138]

Minahk C; Kim KW; Nelson R; Trigatti B; Lehner R; Vance DE. 2008. Conversion of low density lipoprotein-associated phosphatidylcholine to triacylglycerol by primary hepatocytes. J Biol Chem 283(10):6449-58. [PubMed: 18175806]  [MGI Ref ID J:133757]

Nakagawa-Toyama Y; Zhang S; Krieger M. 2012. Dietary manipulation and social isolation alter disease progression in a murine model of coronary heart disease. PLoS One 7(10):e47965. [PubMed: 23112879]  [MGI Ref ID J:192296]

Nguyen DV; Drover VA; Knopfel M; Dhanasekaran P; Hauser H; Phillips MC. 2009. Influence of class B scavenger receptors on cholesterol flux across the brush border membrane and intestinal absorption. J Lipid Res 50(11):2235-44. [PubMed: 19454765]  [MGI Ref ID J:154959]

Nijstad N; Wiersma H; Gautier T; van der Giet M; Maugeais C; Tietge UJ. 2009. Scavenger receptor BI-mediated selective uptake is required for the remodeling of high density lipoprotein by endothelial lipase. J Biol Chem 284(10):6093-100. [PubMed: 19136670]  [MGI Ref ID J:147892]

Out R; Hoekstra M; Spijkers JA; Kruijt JK; van Eck M; Bos IS; Twisk J; Van Berkel TJ. 2004. Scavenger receptor class B type I is solely responsible for the selective uptake of cholesteryl esters from HDL by the liver and the adrenals in mice. J Lipid Res 45(11):2088-95. [PubMed: 15314100]  [MGI Ref ID J:94153]

Out R; Kruijt JK; Rensen PC; Hildebrand RB; de Vos P; Van Eck M; Van Berkel TJ. 2004. Scavenger receptor BI plays a role in facilitating chylomicron metabolism. J Biol Chem 279(18):18401-6. [PubMed: 14970197]  [MGI Ref ID J:124241]

Provost AC; Vede L; Bigot K; Keller N; Tailleux A; Jais JP; Savoldelli M; Ameqrane I; Lacassagne E; Legeais JM; Staels B; Menasche M; Mallat Z; Behar-Cohen F; Abitbol M. 2009. Morphologic and electroretinographic phenotype of SR-BI knockout mice after a long-term atherogenic diet. Invest Ophthalmol Vis Sci 50(8):3931-42. [PubMed: 19420333]  [MGI Ref ID J:154505]

Santander NG; Contreras-Duarte S; Awad MF; Lizama C; Passalacqua I; Rigotti A; Busso D. 2013. Developmental abnormalities in mouse embryos lacking the HDL receptor SR-BI. Hum Mol Genet 22(6):1086-96. [PubMed: 23221804]  [MGI Ref ID J:193292]

Schafer G; Guler R; Murray G; Brombacher F; Brown GD. 2009. The role of scavenger receptor B1 in infection with Mycobacterium tuberculosis in a murine model. PLoS One 4(12):e8448. [PubMed: 20041149]  [MGI Ref ID J:155946]

Seetharam D; Mineo C; Gormley AK; Gibson LL; Vongpatanasin W; Chambliss KL; Hahner LD; Cummings ML; Kitchens RL; Marcel YL; Rader DJ; Shaul PW. 2006. High-density lipoprotein promotes endothelial cell migration and reendothelialization via scavenger receptor-B type I. Circ Res 98(1):63-72. [PubMed: 16339487]  [MGI Ref ID J:118063]

Tanigawa H; Billheimer JT; Tohyama J; Zhang Y; Rothblat G; Rader DJ. 2007. Expression of cholesteryl ester transfer protein in mice promotes macrophage reverse cholesterol transport. Circulation 116(11):1267-73. [PubMed: 17709636]  [MGI Ref ID J:139845]

Thanopoulou K; Fragkouli A; Stylianopoulou F; Georgopoulos S. 2010. Scavenger receptor class B type I (SR-BI) regulates perivascular macrophages and modifies amyloid pathology in an Alzheimer mouse model. Proc Natl Acad Sci U S A 107(48):20816-21. [PubMed: 21076037]  [MGI Ref ID J:167168]

Trigatti B; Rayburn H; Vinals M; Braun A; Miettinen H; Penman M; Hertz M; Schrenzel M; Amigo L; Rigotti A; Krieger M. 1999. Influence of the high density lipoprotein receptor SR-BI on reproductive and cardiovascular pathophysiology. Proc Natl Acad Sci U S A 96(16):9322-7. [PubMed: 10430941]  [MGI Ref ID J:105226]

Van Eck M; Bos IS; Hildebrand RB; Van Rij BT; Van Berkel TJ. 2004. Dual role for scavenger receptor class B, type I on bone marrow-derived cells in atherosclerotic lesion development. Am J Pathol 165(3):785-94. [PubMed: 15331403]  [MGI Ref ID J:92320]

Van Eck M; Hoekstra M; Hildebrand RB; Yaong Y; Stengel D; Kruijt JK; Sattler W; Tietge UJ; Ninio E; Van Berkel TJ; Pratico D. 2007. Increased oxidative stress in scavenger receptor BI knockout mice with dysfunctional HDL. Arterioscler Thromb Vasc Biol 27(11):2413-9. [PubMed: 17717299]  [MGI Ref ID J:134874]

Van Eck M; Hoekstra M; Out R; Bos IS; Kruijt JK; Hildebrand RB; Van Berkel TJ. 2008. Scavenger receptor BI facilitates the metabolism of VLDL lipoproteins in vivo. J Lipid Res 49(1):136-46. [PubMed: 17954936]  [MGI Ref ID J:130302]

Van Eck M; Twisk J; Hoekstra M; Van Rij BT; Van der Lans CA; Bos IS; Kruijt JK; Kuipers F; Van Berkel TJ. 2003. Differential effects of scavenger receptor BI deficiency on lipid metabolism in cells of the arterial wall and in the liver. J Biol Chem 278(26):23699-705. [PubMed: 12639961]  [MGI Ref ID J:84112]

Vishnyakova TG; Kurlander R; Bocharov AV; Baranova IN; Chen Z; Abu-Asab MS; Tsokos M; Malide D; Basso F; Remaley A; Csako G; Eggerman TL; Patterson AP. 2006. CLA-1 and its splicing variant CLA-2 mediate bacterial adhesion and cytosolic bacterial invasion in mammalian cells. Proc Natl Acad Sci U S A 103(45):16888-93. [PubMed: 17071747]  [MGI Ref ID J:117112]

Vrins CL; Ottenhoff R; van den Oever K; de Waart DR; Kruyt JK; Zhao Y; van Berkel TJ; Havekes LM; Aerts JM; van Eck M; Rensen PC; Groen AK. 2012. Trans-intestinal cholesterol efflux is not mediated through high density lipoprotein. J Lipid Res 53(10):2017-23. [PubMed: 22802462]  [MGI Ref ID J:188045]

Wang MD; Kiss RS; Franklin V; McBride HM; Whitman SC; Marcel YL. 2007. Different cellular traffic of LDL-cholesterol and acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways. J Lipid Res 48(3):633-45. [PubMed: 17148552]  [MGI Ref ID J:120283]

Wang X; Collins HL; Ranalletta M; Fuki IV; Billheimer JT; Rothblat GH; Tall AR; Rader DJ. 2007. Macrophage ABCA1 and ABCG1, but not SR-BI, promote macrophage reverse cholesterol transport in vivo. J Clin Invest 117(8):2216-24. [PubMed: 17657311]  [MGI Ref ID J:123959]

West XZ; Malinin NL; Merkulova AA; Tischenko M; Kerr BA; Borden EC; Podrez EA; Salomon RG; Byzova TV. 2010. Oxidative stress induces angiogenesis by activating TLR2 with novel endogenous ligands. Nature 467(7318):972-6. [PubMed: 20927103]  [MGI Ref ID J:165559]

Williams DL; Wong JS; Hamilton RL. 2002. SR-BI is required for microvillar channel formation and the localization of HDL particles to the surface of adrenocortical cells in vivo. J Lipid Res 43(4):544-9. [PubMed: 11907136]  [MGI Ref ID J:75696]

Xie C; Richardson JA; Turley SD; Dietschy JM. 2006. Cholesterol substrate pools and steroid hormone levels are normal in the face of mutational inactivation of NPC1 protein. J Lipid Res 47(5):953-63. [PubMed: 16461760]  [MGI Ref ID J:109000]

Yamamoto S; Tanigawa H; Li X; Komaru Y; Billheimer JT; Rader DJ. 2011. Pharmacologic suppression of hepatic ATP-binding cassette transporter 1 activity in mice reduces high-density lipoprotein cholesterol levels but promotes reverse cholesterol transport. Circulation 124(12):1382-90. [PubMed: 21859969]  [MGI Ref ID J:189383]

Yancey PG; Jerome WG; Yu H; Griffin EE; Cox BE; Babaev VR; Fazio S; Linton MF. 2007. Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI. J Lipid Res 48(5):1140-9. [PubMed: 17299204]  [MGI Ref ID J:121848]

Yesilaltay A; Kocher O; Pal R; Leiva A; Quinones V; Rigotti A; Krieger M. 2006. PDZK1 is required for maintaining hepatic scavenger receptor, class B, type I (SR-BI) steady state levels but not its surface localization or function. J Biol Chem 281(39):28975-80. [PubMed: 16867981]  [MGI Ref ID J:116925]

Yu H; Zhang W; Yancey PG; Koury MJ; Zhang Y; Fazio S; Linton MF. 2006. Macrophage apolipoprotein E reduces atherosclerosis and prevents premature death in apolipoprotein E and scavenger receptor-class BI double-knockout mice. Arterioscler Thromb Vasc Biol 26(1):150-6. [PubMed: 16269665]  [MGI Ref ID J:127962]

Yvan-Charvet L; Pagler TA; Wang N; Senokuchi T; Brundert M; Li H; Rinninger F; Tall AR. 2008. SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL. J Lipid Res 49(1):107-14. [PubMed: 17960026]  [MGI Ref ID J:130277]

Zhang S; Picard MH; Vasile E; Zhu Y; Raffai RL; Weisgraber KH; Krieger M. 2005. Diet-induced occlusive coronary atherosclerosis, myocardial infarction, cardiac dysfunction, and premature death in scavenger receptor class B type I-deficient, hypomorphic apolipoprotein ER61 mice. Circulation 111(25):3457-64. [PubMed: 15967843]  [MGI Ref ID J:114611]

Zhang W; Yancey PG; Su YR; Babaev VR; Zhang Y; Fazio S; Linton MF. 2003. Inactivation of macrophage scavenger receptor class B type I promotes atherosclerotic lesion development in apolipoprotein E-deficient mice. Circulation 108(18):2258-63. [PubMed: 14581413]  [MGI Ref ID J:103014]

Zhang Y; Da Silva JR; Reilly M; Billheimer JT; Rothblat GH; Rader DJ. 2005. Hepatic expression of scavenger receptor class B type I (SR-BI) is a positive regulator of macrophage reverse cholesterol transport in vivo. J Clin Invest 115(10):2870-4. [PubMed: 16200214]  [MGI Ref ID J:101524]

Zhang Y; Yin L; Anderson J; Ma H; Gonzalez FJ; Willson TM; Edwards PA. 2010. Identification of novel pathways that control farnesoid X receptor-mediated hypocholesterolemia. J Biol Chem 285(5):3035-43. [PubMed: 19996107]  [MGI Ref ID J:159763]

Zhao B; Song J; Ghosh S. 2008. Hepatic overexpression of cholesteryl ester hydrolase enhances cholesterol elimination and in vivo reverse cholesterol transport. J Lipid Res 49(10):2212-7. [PubMed: 18599737]  [MGI Ref ID J:142768]

Zhao Y; Pennings M; Hildebrand RB; Ye D; Calpe-Berdiel L; Out R; Kjerrulf M; Hurt-Camejo E; Groen AK; Hoekstra M; Jessup W; Chimini G; Van Berkel TJ; Van Eck M. 2010. Enhanced foam cell formation, atherosclerotic lesion development, and inflammation by combined deletion of ABCA1 and SR-BI in Bone marrow-derived cells in LDL receptor knockout mice on western-type diet. Circ Res 107(12):e20-31. [PubMed: 21071707]  [MGI Ref ID J:178508]

van der Veen JN; Lingrell S; Vance DE. 2012. The membrane lipid phosphatidylcholine is an unexpected source of triacylglycerol in the liver. J Biol Chem 287(28):23418-26. [PubMed: 22610093]  [MGI Ref ID J:188367]

van der Velde AE; Vrins CL; van den Oever K; Seemann I; Oude Elferink RP; van Eck M; Kuipers F; Groen AK. 2008. Regulation of direct transintestinal cholesterol excretion in mice. Am J Physiol Gastrointest Liver Physiol 295(1):G203-G208. [PubMed: 18511744]  [MGI Ref ID J:137834]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, heterozygous mice may be bred with wildtype mice from the colony. Homozygous females are infertile; homozygous males are fertile. Please note that the donating investigator reports that the number of homozygotes resulting from a cross between heterozygotes is significantly lower than what the expected Mendelian ratio would predict. The expected coat color from breeding: Black, Agouti.
Mating System	Heterozygote x +/+ sibling         (Female x Male)   14-SEP-11
	+/+ sibling x Heterozygote         (Female x Male)   07-FEB-11
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering Information
JAX^® Mice
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JAX^® Services
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

Contact information

General inquiries regarding Terms of Use

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.