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Strain Name:

B6;129S2-Scarb1tm1Kri/J

Stock Number:

003379

Availability:

Repository- Live


General Terms and Conditions

Former Name      B6;129S2-Srb1tm1Kri    (Changed: 15-DEC-04 )
Genes & Alleles   Scarb1;   Scarb1tm1Kri;


Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Targeted Mutation
Mating System+/+ sibling x Heterozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Monty Krieger,   Massachusetts Institute of Technology
GenerationF?+18 (08-JAN-08)

Strain Description
The class B, type I scavenger receptor (Srb1 or Scarb1) is a cell surface HDL receptor that can recognize the apolipoproteins on the surface of the HDL particle. It plays a key role in determining the levels of plasma lipoprotein cholesterol (primarily HDL) and the accumulation of cholesterol stores in the adrenal gland.In this strain plasma cholesterol (primarily HDL) concentrations increase by 31% in heterozygotes and 125% in homozygotes, as compared to wild type controls. Also, cholesterol levels in adrenal tissue in heterozygous and homozygous mutants decrease by 42% and 72% respectively, relative to wild type controls. The plasma concentration of Apoa-I, the major protein in HDL, is unchanged in mutant animals, relative to wild type controls.Homozygous females are infertile; homozygous males are fertile. Please note that the donating investigator reports that the number of homozygotes resulting from a cross between heterozygotes is significantly lower than what the expected Mendelian ratio would predict.

Strain Development
The strain was made with replacement of a portion of the first coding exon (including the initiator ATG and sequences encoding a putative membrane spanning region) with a neomycin resistance gene.

Mammalian Phenotype Terms assigned by genotype

Scarb1tm1Kri/Scarb1+

        involves: 129S2/SvPas * C57BL/6
  • homeostasis/metabolism phenotype
  • abnormal lipid homeostasis (MGI Ref ID J:44187)
    • abnormal cholesterol level (MGI Ref ID J:44187)
      • increased circulating cholesterol level (MGI Ref ID J:44187)
        • plasma cholesterol concentration increased by ~31%
  • endocrine/exocrine gland phenotype
  • abnormal adrenal gland physiology (MGI Ref ID J:44187)
    • cholesterol content decreased by 42%

Scarb1tm1Kri/Scarb1tm1Kri

        involves: 129S2/SvPas * C57BL/6
  • homeostasis/metabolism phenotype
  • abnormal lipid homeostasis (MGI Ref ID J:44187)
    • abnormal cholesterol level (MGI Ref ID J:44187)
      • abnormal circulating cholesterol level (MGI Ref ID J:73138)
        • plasma total cholesterol is abnormally high
        • most of the cholesterol is found as abnormally large particles
        • increased circulating cholesterol level (MGI Ref ID J:44187)
          • plasma concentrations are 125% of control values
          • concentration increase is due to formation of large particles containing apolipoprotein A-1
  • endocrine/exocrine gland phenotype
  • abnormal adrenal gland physiology (MGI Ref ID J:44187)
    • cholesterol content is 72% lower than normal
  • lethality-prenatal/perinatal
  • perinatal lethality (MGI Ref ID J:105226)
    • the occasional offspring produced by homozygous mothers die soon after birth
  • prenatal lethality (MGI Ref ID J:105226)
    • the majority of harvested one cell embryos had abnormal,nonrefractile morphology
    • in vitro development of embryos arrests by the 2 cell stage
  • reproductive system phenotype
  • abnormal oocyte morphology (MGI Ref ID J:105226)
    • some superovulated oocytes have an abnormal morphology
  • female infertility (MGI Ref ID J:105226)
    • only occasionally will a pup be born
    • inactivation of the apolipoprotein A-1 gene or administration of the cholesterol-lowering drug probucol restored fertility
    • inactivation of the apolipoprotein A-1 gene or administration of the cholesterol-lowering drug probucol restored fertility
  • hematopoietic system phenotype
  • abnormal erythrocyte morphology (MGI Ref ID J:75090)
    • red blood cells with cytoplasmic irregularities
    • mitochondria present in about 15% of red blood cells
    • macrocytosis (MGI Ref ID J:75090)
    • poikilocytosis (MGI Ref ID J:75090)
      • sometimes
  • anemia (MGI Ref ID J:75090)
    • mild
    • exacerbated on a high cholesterol diet

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Scarb1tm1Kri/Scarb1tm1Kri

        B6.129S2-Scarb1tm1Kri
  • reproductive system phenotype
  • female infertility (MGI Ref ID J:73138)
    • only occasionally is a pup born

Scarb1tm1Kri/Scarb1tm1Kri

        involves: 129S2/SvPas
  • cardiovascular system phenotype
  • abnormal blood vessel healing (MGI Ref ID J:118063)
    • reendothelialization is delayed compared to in wild type mice following injury to the carotid artery
    • mice exhibit a 44% larger area of non-reendothelialized carotid artery following injury compared to in wild type mice
  • homeostasis/metabolism phenotype
  • abnormal blood vessel healing (MGI Ref ID J:118063)
    • reendothelialization is delayed compared to in wild type mice following injury to the carotid artery
    • mice exhibit a 44% larger area of non-reendothelialized carotid artery following injury compared to in wild type mice

Gene & Allele Details

Allele Symbol Scarb1tm1Kri
Allele Name targeted mutation 1, Monty Krieger
Common Name(s) SR-BI; SR-BI-; srb1-; srbI-;
Mutation Made By Monty Krieger,   Massachusetts Institute of Technology
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Scarb1, scavenger receptor class B, member 1
Chromosome 5
Gene Common Name(s) AI120173; CD36L1; CLA-1; CLA1; D5Ertd460e; DNA segment, Chr 5, ERATO Doi 460, expressed; MGC138242; SR-BI; SRB1; SRBI; Srb1; expressed sequence AI120173; scavenger receptor class B1;
Molecular Note Replacement of the entire coding region of the first exon and an additional 554 bases of intron 1 with a neomycin cassette. [MGI Ref ID J:44187]

Control Information

  Allele   Control
 Scarb1tm1Kri  Wild-type from the colony
 
  Considerations for Choosing Controls

Genotyping Protocols

Scarb1tm1Kri

Colony Maintenance

Breeding & HusbandryThe strain originated on a mixed B6,129 background and is currently on the same background. Homozygous females are infertile; homozygous males are fertile. Please note that the donating investigator reports that the number of homozygotes resulting from a cross between heterozygotes is significantly lower than what the expected Mendelian ratio would predict. Expected coat color from breeding:Black, Agouti
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Scarb1tm1Kri allele
004362   B6;129-Scarb1tm1Kri Apoetm1Unc/J
View Strains carrying   Scarb1tm1Kri     (1 strain)

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Scarb1tm1Kri related

Cardiovascular Research
Hypercholesterolemia

Metabolism Research
Lipid Metabolism

Reproductive Biology Research
Fertility Defects (females only)

References

Selected Reference(s)

Rigotti A; Trigatti BL; Penman M; Rayburn H; Herz J; Krieger M. 1997. A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism. Proc Natl Acad Sci U S A 94(23):12610-5. [PubMed: 9356497]  [MGI Ref ID J:44187]

Additional References

Price and Supply Information

Strain Name: B6;129S2-Scarb1tm1Kri/J
Stock Number: 003379

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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