Strain Name: |
B6;129S2-Scarb1tm1Kri/J |
|---|---|
Stock Number: |
003379 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6;129S2-Srb1tm1Kri (Changed: 15-DEC-04
) |
| Genes & Alleles | Scarb1; Scarb1tm1Kri; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Monty Krieger, Massachusetts Institute of Technology Generation F?+18 (08-JAN-08) Strain Description
The class B, type I scavenger receptor (Srb1 or Scarb1) is a cell surface HDL receptor that can recognize the apolipoproteins on the surface of the HDL particle. It plays a key role in determining the levels of plasma lipoprotein cholesterol (primarily HDL) and the accumulation of cholesterol stores in the adrenal gland.In this strain plasma cholesterol (primarily HDL) concentrations increase by 31% in heterozygotes and 125% in homozygotes, as compared to wild type controls. Also, cholesterol levels in adrenal tissue in heterozygous and homozygous mutants decrease by 42% and 72% respectively, relative to wild type controls. The plasma concentration of Apoa-I, the major protein in HDL, is unchanged in mutant animals, relative to wild type controls.Homozygous females are infertile; homozygous males are fertile. Please note that the donating investigator reports that the number of homozygotes resulting from a cross between heterozygotes is significantly lower than what the expected Mendelian ratio would predict.Strain Development
The strain was made with replacement of a portion of the first coding exon (including the initiator ATG and sequences encoding a putative membrane spanning region) with a neomycin resistance gene.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Scarb1tm1Kri | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Monty Krieger | ||
| Common Name(s) | SR-BI; SR-BI-; srb1-; srbI-; | ||
| Mutation Made By | Monty Krieger, Massachusetts Institute of Technology | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Scarb1, scavenger receptor class B, member 1 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | AI120173; CD36L1; CLA-1; CLA1; D5Ertd460e; DNA segment, Chr 5, ERATO Doi 460, expressed; MGC138242; SR-BI; SRB1; SRBI; Srb1; expressed sequence AI120173; scavenger receptor class B1; | ||
| Molecular Note | Replacement of the entire coding region of the first exon and an additional 554 bases of intron 1 with a neomycin cassette. [MGI Ref ID J:44187] | ||
Control Information
| Allele | Control | |
|---|---|---|
| Scarb1tm1Kri | Wild-type from the colony | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Scarb1tm1Kri
Colony Maintenance
| Breeding & Husbandry | The strain originated on a mixed B6,129 background and is currently on the same background. Homozygous females are infertile; homozygous males are fertile. Please note that the donating investigator reports that the number of homozygotes resulting from a cross between heterozygotes is significantly lower than what the expected Mendelian ratio would predict. Expected coat color from breeding:Black, Agouti |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Scarb1tm1Kri allele
004362 B6;129-Scarb1tm1Kri Apoetm1Unc/J View Strains carrying Scarb1tm1Kri (1 strain)
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:Scarb1tm1Kri related
Cardiovascular Research
Hypercholesterolemia
Metabolism Research
Lipid Metabolism
Reproductive Biology Research
Fertility Defects (females only)
References
Selected Reference(s)
Additional ReferencesRigotti A; Trigatti BL; Penman M; Rayburn H; Herz J; Krieger M. 1997. A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism. Proc Natl Acad Sci U S A 94(23):12610-5. [PubMed: 9356497] [MGI Ref ID J:44187]
Price and Supply Information
| Strain Name: | B6;129S2-Scarb1tm1Kri/J |
| Stock Number: | 003379 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form