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Strain Name:

B6;SJL-Tg(aP2-SREBF1c)9884Reh/J

Stock Number:

003393

Availability:

Repository-Cryopreserved


General Terms and Conditions

Genes & Alleles   Fabp4;   SREBF1;   Tg(aP2-SREBF1c)9884Reh;


Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Robert Hammer,   U. of Texas, SW Medical Center at Dallas
GenerationN?+4F1N1p

Strain Description
This transgenic mouse strain overexpresses human nuclear sterol regulatory element binding protein-1c in adipose tissue under the control of the adipocyte-specific aP2 promoter. The phenotype of transgenic mice resembles congenital generalized lipodystrophy (CGL), a rare autosomal recessive disorder in humans. CGL is characterized by an insufficiency of adipose tissue which is evident at birth and is accompanied by a severe insulin resistance leading to symptoms of type II diabetes mellitis, (hyperinsulinemia and hyperglycemia), and an enlarged fatty liver. Transgenic mice exhibit these symptoms showing defects in differentiation of white fat accompanied by an hypertrophy of brown fat that resembles immature white fat.

Strain Development
A transgene was prepared encoding amino acids 1 - 436 of human SREBF1c driven by a 5.4 kb DNA fragment containing the adipose specific enhancer/promoter of the gene encoding aP2. The truncated SREBF1c lacks the membrane-attachment domain and enters the nucleus directly without a requirement for proteolysis. The Founder Line is 988-4, designated as 9884 as a "-" cannot be used in transgenic nomenclature.

Related Disease (OMIM) Terms

Diabetes Mellitus, Noninsulin-Dependent; NIDDM
Lipodystrophy, Congenital Generalized, Type 2; CGL2
Mammalian Phenotype Terms assigned by genotype

Tg(aP2-SREBF1c)9884Reh/0

        involves: C57BL/6J * SJL
  • adipose tissue phenotype
  • *normal* adipose tissue phenotype (MGI Ref ID J:125992)
    • despite adipose tissue defects, adipogenesis is normal in embryonic fibroblasts
    • abnormal adipose tissue morphology (MGI Ref ID J:50770)
      • disordered differentiation of adipose tissue
      • hypertrophic brown fat containing fat-laden cells that resembled white fat
      • decreased white adipose tissue amount (MGI Ref ID J:50770)
  • homeostasis/metabolism phenotype
  • hyperglycemia (MGI Ref ID J:50770)
  • increased circulating insulin level (MGI Ref ID J:50770)
    • 60-fold increase in plasma insulin levels relative to control
  • increased circulating triglyceride level (MGI Ref ID J:50770)
  • insulin resistance (MGI Ref ID J:50770)
  • liver/biliary system phenotype
  • hepatic steatosis (MGI Ref ID J:50770)
    • evident from birth

Gene & Allele Details

Allele Symbol Tg(aP2-SREBF1c)9884Reh
Allele Name transgene insertion 9884, Robert E Hammer
Common Name(s) aP2-SREBP-1c436;
Mutation Made By Robert Hammer,   U. of Texas, SW Medical Center at Dallas
Strain of Origin(C57BL/6J x SJL)F2
Expressed Gene SREBF1, sterol regulatory element binding transcription factor 1, human
Promoter Fabp4, fatty acid binding protein 4, adipocyte, mouse, laboratory
General Note This line was generated from founder number 988-4.
Molecular Note Sequence encoding residues 1 through 436 of human nuclear sterol regulatory element binding protein-1c (SREBF1c) was adjoined to 5.4 kb of the mouse adipose-specific enhancer/promoter of aP2. SREBF1c sequence encoding the membrane-attachment domain was not included in the construct, permitting the expressed componen to enter the nucleus without a requirement for proteolysis. [MGI Ref ID J:50770]

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Genotyping Protocols

Human Growth Hormone Polyadenylation Signal Sequence
Tg(SREBP1a)7343Reh

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Fabp4
005069   B6.Cg-Tg(Fabp4-cre)1Rev/J
004100   FVB-Tg(AZIP/F)1Vsn/J
View Strains carrying other alleles of Fabp4     (2 strains)

Strains carrying other alleles of SREBF1
002840   B6SJL-Tg(SREBP1a)7343Reh/J
View Strains carrying other alleles of SREBF1     (1 strain)

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Hyperglycemia
Hyperinsulinemia
Insulin Resistance
Type 2 Diabetes (NIDDM)

Internal/Organ Research
Adipose Defects
Liver Defects

SREBF1 related

Metabolism Research
Lipid Metabolism

References

Selected Reference(s)

Shimomura I; Hammer RE; Richardson JA; Ikemoto S; Bashmakov Y; Goldstein JL; Brown MS. 1998. Insulin resistance and diabetes mellitus in transgenic mice expressing nuclear SREBP-1c in adipose tissue: model for congenital generalized lipodystrophy. Genes Dev 12(20):3182-94. [PubMed: 9784493]  [MGI Ref ID J:50770]

Additional References

Price and Supply Information

Strain Name: B6;SJL-Tg(aP2-SREBF1c)9884Reh/J
Stock Number: 003393

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryopreserved Embryos
This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page.
Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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