Strain Name: |
B6;SJL-Tg(aP2-SREBF1c)9884Reh/J |
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Stock Number: |
003393 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Fabp4; SREBF1; Tg(aP2-SREBF1c)9884Reh; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Robert Hammer, U. of Texas, SW Medical Center at Dallas Generation N?+4F1N1p Strain Description
This transgenic mouse strain overexpresses human nuclear sterol regulatory element binding protein-1c in adipose tissue under the control of the adipocyte-specific aP2 promoter. The phenotype of transgenic mice resembles congenital generalized lipodystrophy (CGL), a rare autosomal recessive disorder in humans. CGL is characterized by an insufficiency of adipose tissue which is evident at birth and is accompanied by a severe insulin resistance leading to symptoms of type II diabetes mellitis, (hyperinsulinemia and hyperglycemia), and an enlarged fatty liver. Transgenic mice exhibit these symptoms showing defects in differentiation of white fat accompanied by an hypertrophy of brown fat that resembles immature white fat.Strain Development
A transgene was prepared encoding amino acids 1 - 436 of human SREBF1c driven by a 5.4 kb DNA fragment containing the adipose specific enhancer/promoter of the gene encoding aP2. The truncated SREBF1c lacks the membrane-attachment domain and enters the nucleus directly without a requirement for proteolysis. The Founder Line is 988-4, designated as 9884 as a "-" cannot be used in transgenic nomenclature.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(aP2-SREBF1c)9884Reh | ||
|---|---|---|---|
| Allele Name | transgene insertion 9884, Robert E Hammer | ||
| Common Name(s) | aP2-SREBP-1c436; | ||
| Mutation Made By | Robert Hammer, U. of Texas, SW Medical Center at Dallas | ||
| Strain of Origin | (C57BL/6J x SJL)F2 | ||
| Expressed Gene | SREBF1, sterol regulatory element binding transcription factor 1, human | ||
| Promoter | Fabp4, fatty acid binding protein 4, adipocyte, mouse, laboratory | ||
| General Note | This line was generated from founder number 988-4. | ||
| Molecular Note | Sequence encoding residues 1 through 436 of human nuclear sterol regulatory element binding protein-1c (SREBF1c) was adjoined to 5.4 kb of the mouse adipose-specific enhancer/promoter of aP2. SREBF1c sequence encoding the membrane-attachment domain was not included in the construct, permitting the expressed componen to enter the nucleus without a requirement for proteolysis. [MGI Ref ID J:50770] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Human Growth Hormone Polyadenylation Signal Sequence
Tg(SREBP1a)7343Reh
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
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Related Strains
Strains carrying other alleles of Fabp4
005069 B6.Cg-Tg(Fabp4-cre)1Rev/J 004100 FVB-Tg(AZIP/F)1Vsn/J View Strains carrying other alleles of Fabp4 (2 strains)
002840 B6SJL-Tg(SREBP1a)7343Reh/J
Research Applications
This mouse can be used to support research in many areas including:
SREBF1 relatedDiabetes and Obesity Research
Hyperglycemia
Hyperinsulinemia
Insulin Resistance
Type 2 Diabetes (NIDDM)
Internal/Organ Research
Adipose Defects
Liver Defects
Metabolism Research
Lipid Metabolism
References
Selected Reference(s)
Additional ReferencesShimomura I; Hammer RE; Richardson JA; Ikemoto S; Bashmakov Y; Goldstein JL; Brown MS. 1998. Insulin resistance and diabetes mellitus in transgenic mice expressing nuclear SREBP-1c in adipose tissue: model for congenital generalized lipodystrophy. Genes Dev 12(20):3182-94. [PubMed: 9784493] [MGI Ref ID J:50770]
Price and Supply Information
| Strain Name: | B6;SJL-Tg(aP2-SREBF1c)9884Reh/J |
| Stock Number: | 003393 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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