Strain Name: |
C57BL/6-Tg(Zp3-cre)3Mrt/J |
|---|---|
Stock Number: |
003394 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
C57BL/6-TgN(Zp3-Cre)3Mrt (Changed: 15-DEC-04
) |
|
C57BL/6-TgN(Zp3-cre)3Mrt (Changed: 15-DEC-04
) | |
| Genes & Alleles | Zp3; cre; Tg(Zp3-cre)3Mrt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Gail Martin, University of California San Francisco Generation N16 (08-JAN-04) Strain Description
This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. Homozygotes females produce small litters (3.7 pups/litter; Genesis 26:110-112, 2000). An alternative transgenic strain that bears a similar transgenic construct and produces larger litters (~6 pups/litter) is available: C57BL/6-Tg(Zp3-cre)93Knw/J (Stock No. 003651).Strain Development
A CreZp3 construct was injected into FVB/N zygotes to produce the founder line. The strain originated on an FVB/N background. This strain is at N10 on a C57BL/6 background. Homozygotes are viable, but subfertile.
Gene & Allele Details
| Allele Symbol | Tg(Zp3-cre)3Mrt | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, Gail R Martin | ||
| Common Name(s) | Zp3-cre; delta-R; | ||
| Mutation Made By | Gail Martin, University of California San Francisco | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | prior to first meiotic division | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Zp3, zona pellucida glycoprotein 3, mouse, laboratory | ||
| General Note | Homozygous transgenic mice are viable and fertile. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a mouse zona pellucida 3 promoter. This promoter is active in oocytes. No expression was detected in somatic tissues. [MGI Ref ID J:50993] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | The strain originated on an FVB/N background. This strain is at N10 on a C57BL/6 background. Homozygotes are subfertile. Expected coat color from breeding:Black |
|---|
Related Strains
Strains carrying Tg(Zp3-cre)3Mrt allele
003377 FVB/N-Tg(Zp3-cre)3Mrt/J View Strains carrying Tg(Zp3-cre)3Mrt (1 strain)
004038 B6.129S4-Zp3tm1Dean/J 006888 C57BL/6-Tg(Zp3-cre)1Gwh/J 003651 C57BL/6-Tg(Zp3-cre)93Knw/J 006129 STOCK Tg(Zp3-EGFP)1Dean/J
Additional Web Information
Cre-lox or FLP-FRT Systems
JAX Notes, Summer 2001; 482. Cre Transgenic Strains for Conditional Mutagenesis.
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Reproductive Biology Research (Cre-lox System)
Reproductive Biology Research (oocyte)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesLewandoski M; Wassarman KM; Martin GR. 1997. Zp3-cre, a transgenic mouse line for the activation or inactivation of loxP-flanked target genes specifically in the female germ line. Curr Biol 7(2):148-51. [PubMed: 9016703] [MGI Ref ID J:50993]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(Zp3-cre)3Mrt/J |
| Stock Number: | 003394 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form