Description

Strain Information

Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation F?+3   Donating Investigator Ian Dube,   Sunnybrook Health Sciences Centre

Description
This transgenic strain is a good tool for developing therapies for non-Hodgkins lymphoma. Heterozygous mice appear normal and healthy at birth but die in their second year of life. More than 85% of of these mice die from mature B cell lymphoma. No homozygous TLX1 mice were identified in offspring of heterozygous matings, suggesting that homozygotes are not viable.

Related Strains

Strains carrying other alleles of Igh

007594	B6.129P2-Ptrpca Ightm1Mnz/J
008332	C.129S1-Ightm1Janz/J
002595	C57BL/6-Tg(IghelMD4)4Ccg/J
007775	CBy.129P2(B6)-Ightm1Mnz/J
006345	NOD.B6-Tg(IghelMD4)4Ccg/DvsJ
006608	NOD.Cg-Igh-6tm1Cgn Tg(IghelMD4)4Ccg/DvsJ

View Strains carrying other alleles of Igh     (6 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Increased Tumor Incidence (Lymphomas: B cell lymphomas)

Cell Biology Research
Transcriptional Regulation

Developmental Biology Research
Embryonic Lethality (Homozygous)
Postnatal Mortality (Homozygous)

Hematological Research
Hematopoietic Defects

Research Tools
Immunology and Inflammation Research (B cell lymphomas)

Igh related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(Igh-HOX11)11Idd
Allele Name		transgene insertion 11, Ian Dube
Allele Type		Transgenic (random, expressed)
Strain of Origin		CD-1
Expressed Gene		TLX1, T-cell leukemia homeobox 1, human
Promoter		Igh, immunoglobulin heavy chain complex, mouse, laboratory
General Note		Hemizygous mice appear normal and healthy at birth but die in their second year of life. More than 85% of of these mice die from mature B cell lymphoma. No homozygous TLX1 mice were identified in offspring of heterozygous matings, suggesting that homozygotes are not viable.
Molecular Note		Human HOX11 cDNA was placed under the control of the murine Igh-V region promoter fused to the IgH enhancer. Expression of HOX11 was detected in the kidney via Northern blot analysis and in bone marrow and speen tissue via RNAse protection. Founder mice carried between 2 and 4 copies of the transgenic construct. [MGI Ref ID J:52782]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Igh-HOX11)11Idd, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Hough MR; Reis MD; Singaraja R; Bryce DM; Kamel-Reid S; Dardick I; Breitman ML; Dube ID. 1998. A model for spontaneous B-lineage lymphomas in IgHmu-HOX11 transgenic mice. Proc Natl Acad Sci U S A 95(23):13853-8. [PubMed: 9811890]  [MGI Ref ID J:52782]

Additional References

Rosic-Kablar S; Chan K; Reis MD; Dube ID; Hough MR. 2000. Induction of tolerance to immunogenic tumor antigens associated with lymphomagenesis in HOX11 transgenic mice. Proc Natl Acad Sci U S A 97(24):13300-5. [PubMed: 11069299]  [MGI Ref ID J:65884]

Tg(Igh-HOX11)11Idd related

Chen E; Lim MS; Rosic-Kablar S; Liu J; Jolicoeur P; Dube ID; Hough MR. 2006. Dysregulated expression of mitotic regulators is associated with B-cell lymphomagenesis in HOX11-transgenic mice. Oncogene 25(18):2575-87. [PubMed: 16407851]  [MGI Ref ID J:108780]

Rosic-Kablar S; Chan K; Reis MD; Dube ID; Hough MR. 2000. Induction of tolerance to immunogenic tumor antigens associated with lymphomagenesis in HOX11 transgenic mice. Proc Natl Acad Sci U S A 97(24):13300-5. [PubMed: 11069299]  [MGI Ref ID J:65884]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

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