Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+3 Generation Definitions   Donating Investigator Ian Dube,   Sunnybrook Health Sciences Centre

Description
This transgenic strain is a good tool for developing therapies for non-Hodgkins lymphoma. Heterozygous mice appear normal and healthy at birth but die in their second year of life. More than 85% of of these mice die from mature B cell lymphoma. No homozygous TLX1 mice were identified in offspring of heterozygous matings, suggesting that homozygotes are not viable.

Related Strains

Strains carrying other alleles of Igh-V

005020	NOD-Tg(Igh-6/Igh-V281)3Jwt/JwtJ
005019	NOD.Cg-Tg(Igh-6/Igh-V125)2Jwt/JwtJ
005309	NODCaj.Cg-Ighmtm1Cgn Tg(Igh-VB1-8/Igh-6)2Mjsk/FswJ
005306	NODCaj.Cg-Ighmtm1Cgn Tg(Igh-VB1-8/Igh-6m)1Mjsk/FswJ

View Strains carrying other alleles of Igh-V     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Tg(Igh-HOX11)11Idd/0

        CD-1

• mortality/aging
• premature death
  • mice die from lymphoma between 10-20 months of age with a mean around 14-15 months   (MGI Ref ID J:52782)
• tumorigenesis
• B cell derived lymphoma
  • B cell derived lymphoma develops in mice over 10 months of age   (MGI Ref ID J:52782)
  • the lymphoma consists of IgM⁺ and IgD⁺ B cells   (MGI Ref ID J:52782)
  • 88% of the mice have the spleen as the principle organ involved, with the lymphoma infiltrating and destroying the red pulp   (MGI Ref ID J:52782)
  • 25% percent of mice have enlarged thymuses with disruption of the general architecture and extensive replacement of the normal cortex and medulla with lymphoma cells   (MGI Ref ID J:52782)
  • disseminated lymphoma occurs in about 19% of mice with involvement of the kidneys, lungs, liver and pancreas   (MGI Ref ID J:52782)
• immune system phenotype
• abnormal immunoglobulin V(D)J recombination
  • clonal expansion of B cell lymphomas leads to a dominant or co-dominant V-D-J recombination instead of the diverse V-D-J recombinations observed in control mice   (MGI Ref ID J:52782)
• increased leukocyte cell number
  • Mac-1⁺ myeloid cell numbers are significantly increased in older mice with lymphoma   (MGI Ref ID J:52782)
  • increases in this cell population are noted in the bone marrow and lymph nodes of mice with lymphoma   (MGI Ref ID J:52782)
• myeloid hyperplasia
  • myeloid hyperplasia was observed in one 6 month old mouse   (MGI Ref ID J:52782)
  • myeloid hyperplasia occurs in 30% of mice by the time they are terminally ill with lymphoma with 6% of these having no lymphoid involvement of other tissues   (MGI Ref ID J:52782)
• spleen hyperplasia
  • one 3 week old mouse displayed minor spleen hyperplasia   (MGI Ref ID J:52782)
  • at 6 months of age, 3 of 4 mice had spleen hyperplasia with one of these mice having infiltration of the red pulp   (MGI Ref ID J:52782)
• hematopoietic system phenotype
• abnormal immunoglobulin V(D)J recombination
  • clonal expansion of B cell lymphomas leads to a dominant or co-dominant V-D-J recombination instead of the diverse V-D-J recombinations observed in control mice   (MGI Ref ID J:52782)
• increased leukocyte cell number
  • Mac-1⁺ myeloid cell numbers are significantly increased in older mice with lymphoma   (MGI Ref ID J:52782)
  • increases in this cell population are noted in the bone marrow and lymph nodes of mice with lymphoma   (MGI Ref ID J:52782)
• myeloid hyperplasia
  • myeloid hyperplasia was observed in one 6 month old mouse   (MGI Ref ID J:52782)
  • myeloid hyperplasia occurs in 30% of mice by the time they are terminally ill with lymphoma with 6% of these having no lymphoid involvement of other tissues   (MGI Ref ID J:52782)
• spleen hyperplasia
  • one 3 week old mouse displayed minor spleen hyperplasia   (MGI Ref ID J:52782)
  • at 6 months of age, 3 of 4 mice had spleen hyperplasia with one of these mice having infiltration of the red pulp   (MGI Ref ID J:52782)
• cellular phenotype
• abnormal immunoglobulin V(D)J recombination
  • clonal expansion of B cell lymphomas leads to a dominant or co-dominant V-D-J recombination instead of the diverse V-D-J recombinations observed in control mice   (MGI Ref ID J:52782)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Increased Tumor Incidence
      Lymphomas
      Lymphomas: B cell lymphomas

Cell Biology Research
Transcriptional Regulation

Developmental Biology Research
Embryonic Lethality (Homozygous)
Postnatal Lethality
      Homozygous

Hematological Research
Hematopoietic Defects

Research Tools
Immunology and Inflammation Research
      B cell lymphomas

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tg(Igh-HOX11)11Idd
Allele Name		transgene insertion 11, Ian Dube
Allele Type		Transgenic (random, expressed)
Common Name(s)		IgHmu-HOX11Tg;
Mutation Made By		Margaret Hough,   Sunnybrook/Women's College Health Sci Ct
Strain of Origin		CD-1
Expressed Gene		TLX1, T-cell leukemia homeobox 1, human
Promoter		Igh-V, immunoglobulin heavy chain variable region, mouse, laboratory
Molecular Note		Human HOX11 cDNA was placed under the control of the murine Igh-V region promoter fused to the IgH enhancer. Expression of HOX11 was detected in the kidney via Northern blot analysis and in bone marrow and speen tissue via RNAse protection. Founder mice carried between 2 and 4 copies of the transgenic construct. [MGI Ref ID J:52782]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Igh-HOX11)11Idd, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hough MR; Reis MD; Singaraja R; Bryce DM; Kamel-Reid S; Dardick I; Breitman ML; Dube ID. 1998. A model for spontaneous B-lineage lymphomas in IgHmu-HOX11 transgenic mice. Proc Natl Acad Sci U S A 95(23):13853-8. [PubMed: 9811890]  [MGI Ref ID J:52782]

Additional References

Rosic-Kablar S; Chan K; Reis MD; Dube ID; Hough MR. 2000. Induction of tolerance to immunogenic tumor antigens associated with lymphomagenesis in HOX11 transgenic mice. Proc Natl Acad Sci U S A 97(24):13300-5. [PubMed: 11069299]  [MGI Ref ID J:65884]

Tg(Igh-HOX11)11Idd related

Chen E; Kwon YT; Lim MS; Dube ID; Hough MR. 2006. Loss of Ubr1 promotes aneuploidy and accelerates B-cell lymphomagenesis in TLX1/HOX11-transgenic mice. Oncogene 25(42):5752-63. [PubMed: 16862188]  [MGI Ref ID J:147402]

Chen E; Lim MS; Rosic-Kablar S; Liu J; Jolicoeur P; Dube ID; Hough MR. 2006. Dysregulated expression of mitotic regulators is associated with B-cell lymphomagenesis in HOX11-transgenic mice. Oncogene 25(18):2575-87. [PubMed: 16407851]  [MGI Ref ID J:108780]

Rosic-Kablar S; Chan K; Reis MD; Dube ID; Hough MR. 2000. Induction of tolerance to immunogenic tumor antigens associated with lymphomagenesis in HOX11 transgenic mice. Proc Natl Acad Sci U S A 97(24):13300-5. [PubMed: 11069299]  [MGI Ref ID J:65884]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.
• Genomic DNA is available for this strain from the Mouse DNA Resource.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.