Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F+2p

Description
C57BL/6-TgN(Amy1TAg)354Knw mice are viable and fertile. They express the SV40 tumor antigen under control of the liver promoter of the mouse alpha-amylase gene (Amy-1^a). This transgenic strain develops malignant tumors of the brown adipose tissue at about 18 months of age. The tumors have high metastatic potential, with metastases commonly occurring in liver, lung, spleen, heart and adrenal glands. Note: This line, '354', contains the same trangene construct as line '501' (Stock No. 003446). Divergent tumor development between these lines is reported to be a result of different transgene integration sites.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Amy1

000628	B6.CE-A Amy1b Amy2a5b/J
003446	C57BL/6J-Tg(Amy1TAg)501Knw/J
000187	LT.CAST-Amy1b/J

View Strains carrying other alleles of Amy1     (3 strains)

Strains carrying other alleles of TAg

008215	(C57BL/6-Tg(TRAMP)8247Ng/J X FVB/NJ)F1/J
003382	B10.D2-Tg(C3-1-TAg)cJeg/J
008247	B6.Cg-Tg(Ela1-TAg*)289Mjt/J
003380	B6.FVB-Tg(C3-1-TAg)cJeg/J
003135	C57BL/6-Tg(TRAMP)8247Ng/J
003446	C57BL/6J-Tg(Amy1TAg)501Knw/J
003477	C57BL/6J-Tg(SV)419Bri/J
003476	C57BL/6J-Tg(SV)427Bri/J
002233	C57BL/6J-Tg(SV)7Bri/J
003189	C57BL/6J-Tg(WAPTAg)3Knw/J
003188	C57BL/6J-Tg(WapTAg)1Knw
005564	FVB(Cg)-Tg(Ins2-CALM1)26Ove Tg(Cryaa-TAg)1Ove/PneJ
003381	FVB-Tg(C3-1-TAg)cJeg/J
002380	NOD.Cg-Tg(Ins2-TAg)1Lt Prkdcscid/DvsJ
002033	NOD/ShiLt-Tg(RipTAg)1Lt/J

View Strains carrying other alleles of TAg     (15 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(Amy1TAg)354Knw/0

        C57BL/6J-Tg(Amy1TAg)354Knw

• tumorigenesis
• increased tumor incidence (MGI Ref ID J:90326)
  • 40% of mice develop hibernomas (brown adipose tissue tumors; in 10 of 12 mice) and hepatomas (in 2 of 12 mice) with onset at 15 months of age
  • foci of lipid-containing anaplastic cells are detected in numerous organs including liver, lung, spleen, heart, and adrenals
• hepatoma (MGI Ref ID J:90326)
  • in 2 of 12 mice with tumors

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Other Tissues/Organs: adipose tissue
Other
      tumor metastasis

TAg related

Apoptosis Research
Extracellular Modulators

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(Amy1TAg)354Knw
Allele Name		transgene insertion 354, Barbara Knowles
Allele Type		Transgenic (random, expressed)
Common Name(s)		354;
Strain of Origin		C57BL/6J
Expressed Gene		TAg, SV40 large T-antigen, SV40
		Simian virus 40 T antigen (SV40Tag) is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer.
Promoter		Amy1, amylase 1, salivary, mouse, laboratory
Molecular Note		The transgene encodes an amylase 1 (salivary)-SV40 T-antigen hybrid protein and is expressed in the liver. Transgene expression is controlled by the mouse amylase 1, salivary, gene (Amy1) liver isoform promoter. Line 501 was also produced. [MGI Ref ID J:90326]

Genotyping

Genotyping Information

Genotyping Protocols

TAg, SV, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Fox N; Crooke R; Hwang LH; Schibler U; Knowles BB; Solter D. 1989. Metastatic hibernomas in transgenic mice expressing an alpha-amylase-SV40 T antigen hybrid gene. Science 244(4903):460-3. [PubMed: 2785714]  [MGI Ref ID J:90326]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

Terms of Use

General Terms and Conditions

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Contact information

General inquiries

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fax:	207-288-6655

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