Strain Name:

C57BL/6J-Tg(Amy1TAg)501Knw/J

Stock Number:

003446

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Terms of Use

Description

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
GenerationF?+2p

Description
C57BL/6-TgN(Amy1TAg)501Knw mice are viable and fertile. They express the SV40 tumor antigen under control of the liver promoter of the mouse alpha-amylase gene (Amy1a). This transgenic strain develops metastatic osteosarcomas at about 13 months of age. Note: This line, '501', contains the same trangene constuct as line '354' (Stock No. 003445). Divergent tumor development between these lines is reported to be a result of different transgene integration sites.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Amy1
000628   B6.CE-A Amy1b Amy2b/J
003445   C57BL/6J-Tg(Amy1TAg)354Knw/J
000187   LT.CAST-Amy1b/J
View Strains carrying other alleles of Amy1     (3 strains)

View Strains carrying other alleles of TAg     (15 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Tg(Amy1TAg)501Knw/0

        involves: C57BL/6
  • immune system phenotype
  • abnormal CD8-positive T cell physiology (MGI Ref ID J:84288)
    • T cells from transgenic mice exposed to T antigen respond inefficiently to subsequent immunization with T antigen in T cell transfer experiments
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence (Other Tissues/Organs: osteosarcoma)
Other (tumor metastasis)

TAg related

Apoptosis Research
Extracellular Modulators

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Amy1TAg)501Knw
Allele Name transgene insertion 501, Barbara Knowles
Allele Type Transgenic (random, expressed)
Common Name(s) p600T;
Strain of OriginC57BL/6J
Expressed Gene TAg, SV40 large T-antigen, SV40
Simian virus 40 T antigen (SV40Tag) is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer.
Promoter Amy1, amylase 1, salivary, mouse, laboratory
General Note Line 354 was also produced.

Transgenic mice on a C57BL/6J background are viable and fertile. They develop metastatic osteosarcomas at about 13 months of age. The difference in tumor development between these mice and transgenic mice carrying line 354is reported to be a result of different transgene integration sites.

Molecular Note The transgene encodes an alpha-amylase-SV40 T-antigen hybrid protein under control of the liver promoter of the mouse alpha-amylase 1, salivary, gene. [MGI Ref ID J:90326]

Genotyping

Genotyping Information

Genotyping Protocols

TAg, SV, STD PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Fox N; Crooke R; Hwang LH; Schibler U; Knowles BB; Solter D. 1989. Metastatic hibernomas in transgenic mice expressing an alpha-amylase-SV40 T antigen hybrid gene. Science 244(4903):460-3. [PubMed: 2785714]  [MGI Ref ID J:90326]

Additional References

Knowles BB; McCarrick J; Fox N; Solter D; Damjanov I. 1990. Osteosarcomas in transgenic mice expressing an alpha-amylase-SV40 T-antigen hybrid gene. Am J Pathol 137(2):259-62. [PubMed: 2386195]  [MGI Ref ID J:90730]

Marton I; Johnson SE; Damjanov I; Currier KS; Sundberg JP; Knowles BB. 2000. Expression and immune recognition of SV40 Tag in transgenic mice that develop metastatic osteosarcomas Transgenic Res 9(2):115-25. [PubMed: 10951695]  [MGI Ref ID J:63776]

Tg(Amy1TAg)501Knw related

Knowles BB; McCarrick J; Fox N; Solter D; Damjanov I. 1990. Osteosarcomas in transgenic mice expressing an alpha-amylase-SV40 T-antigen hybrid gene. Am J Pathol 137(2):259-62. [PubMed: 2386195]  [MGI Ref ID J:90730]

Marton I; Johnson SE; Damjanov I; Currier KS; Sundberg JP; Knowles BB. 2000. Expression and immune recognition of SV40 Tag in transgenic mice that develop metastatic osteosarcomas Transgenic Res 9(2):115-25. [PubMed: 10951695]  [MGI Ref ID J:63776]

Schell TD; Knowles BB; Tevethia SS. 2000. Sequential loss of cytotoxic T lymphocyte responses to simian virus 40 large T antigen epitopes in T antigen transgenic mice developing osteosarcomas. Cancer Res 60(11):3002-12. [PubMed: 10850449]  [MGI Ref ID J:62541]

Staveley-O'Carroll K; Schell TD; Jimenez M; Mylin LM; Tevethia MJ; Schoenberger SP; Tevethia SS. 2003. In vivo ligation of CD40 enhances priming against the endogenous tumor antigen and promotes CD8+ T cell effector function in SV40 T antigen transgenic mice. J Immunol 171(2):697-707. [PubMed: 12847236]  [MGI Ref ID J:84288]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


OncoMouse® requires a license from DuPont, see Licenses for Strains with OncoMouse® Technology.

Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

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