Strain Name: |
CByJ.129P2(B6)-Tcrdtm1Mom/Sz |
|---|---|
Stock Number: |
003448 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Strain Common Names | BALB/cByJ-Tcrd; |
| Genes & Alleles | Tcrd; Tcrdtm1Mom; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse H2 Haplotype d Strain Description
Mice homozygous for this targeted mutation are viable and fertile. Gamma delta T-cell receptor expression is deficient in all adult lymphoid and epithelial organs. There is normal development of the alpha beta T-cell lineage. Patterns of CD4+CD8- and CD4-CD8+ alpha beta T-cells are apparently normal. Mice do not develop inflammatory bowel disease.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
This targeted mutant strain was developed by Dr. Peter Mombaerts in the laboratory of Dr. Susumu Tonegawa at the Center for Cancer Research, Massachusetts Institute of Technology. A replacement targeting vector containing Pgk-neo was used which resulted in a 4 kb deletion encompassing most of the delta chain constant region coding sequences. This strain was generated in the laboratory of Dr. Leonard Shultz by backcrossing mice from B6.1292-Tcrdtm1Mom/J to BALB/cByJ inbred mice for a minimum of 9 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tcrdtm1Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Peter Mombaerts | ||
| Common Name(s) | C-delta-; TCRdelta KO; TCRdelta-; delta-; delta0; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129 | ||
| ES Cell Line Name | Other (see notes) | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Tcrd, T-cell receptor delta chain | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | TCRDV1; TRD; Tcr delta; Tcrdelta; | ||
| General Note | ES cell line = E14 (129P2/OlaHsd) or D3 (129S2/SvPas). | ||
| Molecular Note | A PGK-neo cassette was used to disrupt the constant gene segment (designated C-delta). Flow cytometry showed that cells isolated from the lymphoid or epithelial organs of homozygous mice did not react with an anti-pan Tcrd mAb. Immunoprecipitation showedan absence of expression on the surface of thymocytes isolated from homozygous mutant embryos. [MGI Ref ID J:3865] [MGI Ref ID J:91942] | ||
Genotyping Protocols
NEOTD (Generic Neo)
Related Strains
Strains carrying Tcrdtm1Mom allele
002122 B6.129P2-Tcrbtm1Mom Tcrdtm1Mom/J 002120 B6.129P2-Tcrdtm1Mom/J 002121 B6;129P-Tcrbtm1Mom Tcrdtm1Mom/J 002119 B6;129P2-Tcrdtm1Mom/J View Strains carrying Tcrdtm1Mom (4 strains)
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:Tcrdtm1Mom related
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation (Inflammatory bowel disease)
T Cell Receptor Signaling Defects
Research Tools
Immunology and Inflammation Research (T Cell Receptor Deficiency)
References
Selected Reference(s)
Additional ReferencesItohara S; Mombaerts P; Lafaille J; Iacomini J; Nelson A; Clarke AR; Hooper ML; Farr A; Tonegawa S. 1993. T cell receptor delta gene mutant mice: independent generation of alpha beta T cells and programmed rearrangements of gamma delta TCR genes. Cell 72(3):337-48. [PubMed: 8381716] [MGI Ref ID J:3865]
Price and Supply Information
| Strain Name: | CByJ.129P2(B6)-Tcrdtm1Mom/Sz |
| Stock Number: | 003448 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
General Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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