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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;D2-TgN(Sycp1-Cre)4Min (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?+3F2pN2p (26-FEB-06)
Generation DefinitionsDonating Investigator Dr. Minoo Rassoulzadegan, Universite de Nice Development
Transgenic mice were generated expressing a testicular Cre recombinase driven by promoter sequences derived from the gene encoding synaptonemal complex protein 1 (Sycp1), expressed at the leptotene to zygotene stage of male meiosis. The founder line for this strain is #4-30.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100006 B6D2F1/J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (394 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Reproductive Biology Research
Cre-lox System
male germ cells
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Sycp1-cre)4Min | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Minoo Rassoulzadegan | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | SYCP-Cre; Sycp1-Cre; | ||
| Mutation Made By | Dr. Minoo Rassoulzadegan, Universite de Nice | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 | ||
| Site of Expression | male germ cells; expressed at the leptotene-zygotene stage of male meiosis | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Sycp1, synaptonemal complex protein 1, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Sycp1-cre)4Min, transgene insertion 4, Minoo Rassoulzadegan | ||
| Chromosome | UN | ||
| Gene Common Name(s) | SYCP-Cre; Sycp1-Cre; | ||
| Driver Note | Sycp1 | ||
| General Note | The original founder number for this line was #4-30. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a mouse synaptonemal complex protein 1 promoter. This promoter is active at the leptotene to zygotene stage of male meiosis. [MGI Ref ID J:67933] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Sycp1-cre)4Min, Separated PCR
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Vidal F; Sage J; Cuzin F; Rassoulzadegan M. 1998. Cre expression in primary spermatocytes: a tool for genetic engineering of the germ line. Mol Reprod Dev 51(3):274-80. [PubMed: 9771647] [MGI Ref ID J:67933]
Additional References
Tg(Sycp1-cre)4Min relatedBatista F; Lu L; Williams SA; Stanley P. 2012. Complex N-glycans are essential, but core 1 and 2 mucin O-glycans, O-fucose glycans, and NOTCH1 are dispensable, for mammalian spermatogenesis. Biol Reprod 86(6):179. [PubMed: 22492969] [MGI Ref ID J:185823]
Bockamp E; Antunes C; Maringer M; Heck R; Presser K; Beilke S; Ohngemach S; Alt R; Cross M; Sprengel R; Hartwig U; Kaina B; Schmitt S; Eshkind L. 2006. Tetracycline-controlled transgenic targeting from the SCL locus directs conditional expression to erythrocytes, megakaryocytes, granulocytes, and c-kit-expressing lineage-negative hematopoietic cells. Blood 108(5):1533-41. [PubMed: 16675709] [MGI Ref ID J:138070]
Chi MN; Auriol J; Jegou B; Kontoyiannis DL; Turner JM; de Rooij DG; Morello D. 2011. The RNA-binding protein ELAVL1/HuR is essential for mouse spermatogenesis, acting both at meiotic and postmeiotic stages. Mol Biol Cell 22(16):2875-2885. [PubMed: 21737689] [MGI Ref ID J:175695]
Chung SS; Cuzin F; Rassoulzadegan M; Wolgemuth DJ. 2004. Primary spermatocyte-specific Cre recombinase activity in transgenic mice. Transgenic Res 13(3):289-94. [PubMed: 15359605] [MGI Ref ID J:168137]
Eferl R; Hoebertz A; Schilling AF; Rath M; Karreth F; Kenner L; Amling M; Wagner EF. 2004. The Fos-related antigen Fra-1 is an activator of bone matrix formation. EMBO J 23(14):2789-99. [PubMed: 15229648] [MGI Ref ID J:93053]
Fossat N; Chatelain G; Brun G; Lamonerie T. 2006. Temporal and spatial delineation of mouse Otx2 functions by conditional self-knockout. EMBO Rep 7(8):824-30. [PubMed: 16845372] [MGI Ref ID J:116888]
Fumel B; Guerquin MJ; Livera G; Staub C; Magistrini M; Gauthier C; Flamant F; Guillou F; Fouchecourt S. 2012. Thyroid hormone limits postnatal Sertoli cell proliferation in vivo by activation of its alpha1 isoform receptor (TRalpha1) present in these cells and by regulation of Cdk4/JunD/c-myc mRNA levels in mice. Biol Reprod 87(1):16, 1-9. [PubMed: 22539677] [MGI Ref ID J:192440]
Galan-Caridad JM; Harel S; Arenzana TL; Hou ZE; Doetsch FK; Mirny LA; Reizis B. 2007. Zfx controls the self-renewal of embryonic and hematopoietic stem cells. Cell 129(2):345-57. [PubMed: 17448993] [MGI Ref ID J:149654]
Genoud N; Behrens A; Miele G; Robay D; Heppner FL; Freigang S; Aguzzi A. 2004. Disruption of Doppel prevents neurodegeneration in mice with extensive Prnp deletions. Proc Natl Acad Sci U S A 101(12):4198-203. [PubMed: 15007175] [MGI Ref ID J:89232]
Hendershot TJ; Liu H; Sarkar AA; Giovannucci DR; Clouthier DE; Abe M; Howard MJ. 2007. Expression of Hand2 is sufficient for neurogenesis and cell type-specific gene expression in the enteric nervous system. Dev Dyn 236(1):93-105. [PubMed: 17075884] [MGI Ref ID J:138032]
Herault Y; Kmita M; Sawaya CC; Duboule D. 2002. A nested deletion approach to generate Cre deleter mice with progressive Hox profiles. Int J Dev Biol 46(1 Spec No):185-91. [PubMed: 11902682] [MGI Ref ID J:82530]
Herault Y; Rassoulzadegan M; Cuzin F; Duboule D. 1998. Engineering chromosomes in mice through targeted meiotic recombination (TAMERE). Nat Genet 20(4):381-4. [PubMed: 9843213] [MGI Ref ID J:51289]
Kmita M; Tarchini B; Duboule D; Herault Y. 2002. Evolutionary conserved sequences are required for the insulation of the vertebrate Hoxd complex in neural cells. Development 129(23):5521-8. [PubMed: 12403721] [MGI Ref ID J:80084]
Lefebvre L; Mar L; Bogutz A; Oh-McGinnis R; Mandegar MA; Paderova J; Gertsenstein M; Squire JA; Nagy A. 2009. The interval between Ins2 and Ascl2 is dispensable for imprinting centre function in the murine Beckwith-Wiedemann region. Hum Mol Genet 18(22):4255-67. [PubMed: 19684026] [MGI Ref ID J:153719]
Quignodon L; Vincent S; Winter H; Samarut J; Flamant F. 2007. A Point Mutation in the Activation Function 2 Domain of Thyroid Hormone Receptor {alpha}1 Expressed after CRE-Mediated Recombination Partially Recapitulates Hypothyroidism. Mol Endocrinol 21(10):2350-2360. [PubMed: 17622582] [MGI Ref ID J:125353]
Rasoulpour RJ; Boekelheide K. 2007. NF-kappaB activation elicited by ionizing radiation is proapoptotic in testis. Biol Reprod 76(2):279-85. [PubMed: 17123945] [MGI Ref ID J:121120]
Rasoulpour RJ; Boekelheide K. 2006. The Sycp1-Cre transgenic mouse and male germ cell inhibition of NF-kappa b. J Androl 27(6):729-33. [PubMed: 16775250] [MGI Ref ID J:133761]
Souabni A; Cobaleda C; Schebesta M; Busslinger M. 2002. Pax5 promotes B lymphopoiesis and blocks T cell development by repressing Notch1. Immunity 17(6):781-93. [PubMed: 12479824] [MGI Ref ID J:80815]
Spitz F; Herkenne C; Morris MA; Duboule D. 2005. Inversion-induced disruption of the Hoxd cluster leads to the partition of regulatory landscapes. Nat Genet 37(8):889-93. [PubMed: 15995706] [MGI Ref ID J:100298]
Starck J; Weiss-Gayet M; Gonnet C; Guyot B; Vicat JM; Morle F. 2010. Inducible Fli-1 gene deletion in adult mice modifies several myeloid lineage commitment decisions and accelerates proliferation arrest and terminal erythrocytic differentiation. Blood 116(23):4795-805. [PubMed: 20733157] [MGI Ref ID J:167426]
Tsai MY; Yeh SD; Wang RS; Yeh S; Zhang C; Lin HY; Tzeng CR; Chang C. 2006. Differential effects of spermatogenesis and fertility in mice lacking androgen receptor in individual testis cells. Proc Natl Acad Sci U S A 103(50):18975-80. [PubMed: 17142319] [MGI Ref ID J:118270]
Vignier N; Schlossarek S; Fraysse B; Mearini G; Kramer E; Pointu H; Mougenot N; Guiard J; Reimer R; Hohenberg H; Schwartz K; Vernet M; Eschenhagen T; Carrier L. 2009. Nonsense-mediated mRNA decay and ubiquitin-proteasome system regulate cardiac myosin-binding protein C mutant levels in cardiomyopathic mice. Circ Res 105(3):239-48. [PubMed: 19590044] [MGI Ref ID J:164934]
Zacchetti G; Duboule D; Zakany J. 2007. Hox gene function in vertebrate gut morphogenesis: the case of the caecum. Development 134(22):3967-3973. [PubMed: 17942481] [MGI Ref ID J:127046]
Zakany J; Kmita M; Duboule D. 2004. A dual role for Hox genes in limb anterior-posterior asymmetry. Science 304(5677):1669-72. [PubMed: 15192229] [MGI Ref ID J:91567]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry The strain is on a B6D2F1 background. Homozygotes are viable and fertile. The investigator maintains the strain by mating hemizygotes X B6D2F1. Expected coat color from breeding:Black and Dilute Brown Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100006 B6D2F1/J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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