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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;D2-TgN(Sycp1-Cre)4Min (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?+3F2pN2p (26-FEB-06) Donating Investigator Minoo Rassoulzadegan, Universite de Nice Development
Transgenic mice were generated expressing a testicular Cre recombinase driven by promoter sequences derived from the gene encoding synaptonemal complex protein 1 (Sycp1), expressed at the leptotene to zygotene stage of male meiosis. The founder line for this strain is #4-30.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100006 B6D2F1/J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (162 strains)
Additional Web Information
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Reproductive Biology Research
Cre-lox System
male germ cells
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Sycp1-cre)4Min | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Minoo Rassoulzadegan | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | SYCP-Cre; Sycp1-Cre; | ||
| Mutation Made By | Minoo Rassoulzadegan, Universite de Nice | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 | ||
| Site of Expression | male germ cells; expressed at the leptotene-zygotene stage of male meiosis | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Sycp1, synaptonemal complex protein 1, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Sycp1-cre)4Min, transgene insertion 4, Minoo Rassoulzadegan | ||
| Chromosome | UN | ||
| Gene Common Name(s) | SYCP-Cre; Sycp1-Cre; | ||
| Driver Note | Sycp1 | ||
| General Note | The original founder number for this line was #4-30. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a mouse synaptonemal complex protein 1 promoter. This promoter is active at the leptotene to zygotene stage of male meiosis. [MGI Ref ID J:67933] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Vidal F; Sage J; Cuzin F; Rassoulzadegan M. 1998. Cre expression in primary spermatocytes: a tool for genetic engineering of the germ line. Mol Reprod Dev 51(3):274-80. [PubMed: 9771647] [MGI Ref ID J:67933]
Additional References
Tg(Sycp1-cre)4Min relatedBockamp E; Antunes C; Maringer M; Heck R; Presser K; Beilke S; Ohngemach S; Alt R; Cross M; Sprengel R; Hartwig U; Kaina B; Schmitt S; Eshkind L. 2006. Tetracycline-controlled transgenic targeting from the SCL locus directs conditional expression to erythrocytes, megakaryocytes, granulocytes, and c-kit-expressing lineage-negative hematopoietic cells. Blood 108(5):1533-41. [PubMed: 16675709] [MGI Ref ID J:138070]
Eferl R; Hoebertz A; Schilling AF; Rath M; Karreth F; Kenner L; Amling M; Wagner EF. 2004. The Fos-related antigen Fra-1 is an activator of bone matrix formation. EMBO J 23(14):2789-99. [PubMed: 15229648] [MGI Ref ID J:93053]
Fossat N; Chatelain G; Brun G; Lamonerie T. 2006. Temporal and spatial delineation of mouse Otx2 functions by conditional self-knockout. EMBO Rep 7(8):824-30. [PubMed: 16845372] [MGI Ref ID J:116888]
Galan-Caridad JM; Harel S; Arenzana TL; Hou ZE; Doetsch FK; Mirny LA; Reizis B. 2007. Zfx controls the self-renewal of embryonic and hematopoietic stem cells. Cell 129(2):345-57. [PubMed: 17448993] [MGI Ref ID J:149654]
Genoud N; Behrens A; Miele G; Robay D; Heppner FL; Freigang S; Aguzzi A. 2004. Disruption of Doppel prevents neurodegeneration in mice with extensive Prnp deletions. Proc Natl Acad Sci U S A 101(12):4198-203. [PubMed: 15007175] [MGI Ref ID J:89232]
Hendershot TJ; Liu H; Sarkar AA; Giovannucci DR; Clouthier DE; Abe M; Howard MJ. 2007. Expression of Hand2 is sufficient for neurogenesis and cell type-specific gene expression in the enteric nervous system. Dev Dyn 236(1):93-105. [PubMed: 17075884] [MGI Ref ID J:138032]
Herault Y; Kmita M; Sawaya CC; Duboule D. 2002. A nested deletion approach to generate Cre deleter mice with progressive Hox profiles. Int J Dev Biol 46(1 Spec No):185-91. [PubMed: 11902682] [MGI Ref ID J:82530]
Herault Y; Rassoulzadegan M; Cuzin F; Duboule D. 1998. Engineering chromosomes in mice through targeted meiotic recombination (TAMERE). Nat Genet 20(4):381-4. [PubMed: 9843213] [MGI Ref ID J:51289]
Kmita M; Tarchini B; Duboule D; Herault Y. 2002. Evolutionary conserved sequences are required for the insulation of the vertebrate Hoxd complex in neural cells. Development 129(23):5521-8. [PubMed: 12403721] [MGI Ref ID J:80084]
Quignodon L; Vincent S; Winter H; Samarut J; Flamant F. 2007. A Point Mutation in the Activation Function 2 Domain of Thyroid Hormone Receptor {alpha}1 Expressed after CRE-Mediated Recombination Partially Recapitulates Hypothyroidism. Mol Endocrinol 21(10):2350-2360. [PubMed: 17622582] [MGI Ref ID J:125353]
Rasoulpour RJ; Boekelheide K. 2007. NF-kappaB activation elicited by ionizing radiation is proapoptotic in testis. Biol Reprod 76(2):279-85. [PubMed: 17123945] [MGI Ref ID J:121120]
Rasoulpour RJ; Boekelheide K. 2006. The Sycp1-Cre transgenic mouse and male germ cell inhibition of NF-kappa b. J Androl 27(6):729-33. [PubMed: 16775250] [MGI Ref ID J:133761]
Souabni A; Cobaleda C; Schebesta M; Busslinger M. 2002. Pax5 promotes B lymphopoiesis and blocks T cell development by repressing Notch1. Immunity 17(6):781-93. [PubMed: 12479824] [MGI Ref ID J:80815]
Spitz F; Herkenne C; Morris MA; Duboule D. 2005. Inversion-induced disruption of the Hoxd cluster leads to the partition of regulatory landscapes. Nat Genet 37(8):889-93. [PubMed: 15995706] [MGI Ref ID J:100298]
Tsai MY; Yeh SD; Wang RS; Yeh S; Zhang C; Lin HY; Tzeng CR; Chang C. 2006. Differential effects of spermatogenesis and fertility in mice lacking androgen receptor in individual testis cells. Proc Natl Acad Sci U S A 103(50):18975-80. [PubMed: 17142319] [MGI Ref ID J:118270]
Zacchetti G; Duboule D; Zakany J. 2007. Hox gene function in vertebrate gut morphogenesis: the case of the caecum. Development 134(22):3967-3973. [PubMed: 17942481] [MGI Ref ID J:127046]
Zakany J; Kmita M; Duboule D. 2004. A dual role for Hox genes in limb anterior-posterior asymmetry. Science 304(5677):1669-72. [PubMed: 15192229] [MGI Ref ID J:91567]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry The strain is on a B6D2F1 background. Homozygotes are viable and fertile. The investigator maintains the strain by mating hemizygotes X B6D2F1. Expected coat color from breeding:Black and Dilute Brown Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 Cryopreserved Embryos $1600.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 Cryopreserved Embryos $2080.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100006 B6D2F1/J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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