Strain Name:

C57BL/6-Tg(HLA-A2.1)1Enge/J

Stock Number:

003475

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Availability:

Level 4

Use Restrictions Apply, see Terms of Use
Homozygous mice carrying the Tg(HLA-A2.1)1Enge transgene express significant quantities of the human class I MHC Ag HLA-A2.1 on cells from the spleen, bone marrow and thymus. These transgenic mice have been used to identify hepatitic C virus (HCV) peptides expressing a sequence for HLA-A2.1 binding that are actually recognized by human A2.1-restricted CTLs. Thus, this transgenic model is important for the study of HLA-restricted CTL determinants and in potential development of a vaccine against HCV.

Description

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Breeding Considerations This strain is a good breeder.
Specieslaboratory mouse
GenerationF13 (24-SEP-12)
Generation Definitions
 
Donating InvestigatorDr. Victor H. Engelhard,   University of Virginia

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Appearance
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Related Genotype: a/a

Description
Homozygous mice carrying the Tg(HLA-A2.1)1Enge transgene express significant quantities of the human class I MHC Ag HLA-A2.1 on cells from the spleen, bone marrow and thymus. Expression of this human class I molecule did not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. These transgenic mice have been used to identify hepatitic C virus (HCV) peptides expressing a sequence for HLA-A2.1 binding that are actually recognized by human A2.1-restricted CTLs. Thus, this transgenic model is important for the study of HLA-restricted CTL determinants and in potential development of a vaccine against HCV.

Development
Transgenic mice were constructed by microinjection of the 7-kb EcoRI fragment containing the full length HLA-A2.1 gene into fertilized eggs from C57BL/6 mice.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying   Tg(HLA-A2.1)1Enge     (5 strains)

View Strains carrying other alleles of HLA-A     (7 strains)

View Strains carrying other alleles of HLA-A2.1     (6 strains)

Additional Web Information

Technical Bulletin - Monitoring the Expression of the HLA-A2.1 Transgene

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Severe Cutaneous Adverse Reaction, Susceptibility to   (HLA-A)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(HLA-A2.1)1Enge/0

        involves: C57BL/6
  • immune system phenotype
  • abnormal immune tolerance
    • transgenic animals are more tolerant to HLA-A2.1-expressing murine cells than control mice primed once with influenza virus; numbers of cytotoxic T lymphocyte precursors are 100-fold higher in primed C57BL/6 controls   (MGI Ref ID J:52612)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
HLA transgenics

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(HLA-A2.1)1Enge
Allele Name transgene insertion 1, Victor H Engelhard
Allele Type Transgenic (Inserted expressed sequence)
Mutation Made ByDr. Ai-Xuan Le,   Stanford University
Strain of OriginC57BL/6
Expressed Gene HLA-A, major histocompatibility complex, class I, A, human
Promoter HLA-A2.1, major histocompatibility complex, class I, subtype A2.1, human
General Note Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2.
Molecular Note The transgene contains the full length HLA-A2.1 gene. Expression of human class I MHC Ag HLA-A2.1 was detected on cells from homozygous transgenic spleen, bone marrow, and thymus. [MGI Ref ID J:52612]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(HLA-A/H2-D), QPCR
Tg(HLA-A2.1)1Enge, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Le AX; Bernhard EJ; Holterman MJ; Strub S; Parham P; Lacy E; Engelhard VH. 1989. Cytotoxic T cell responses in HLA-A2.1 transgenic mice. Recognition of HLA alloantigens and utilization of HLA-A2.1 as a restriction element. J Immunol 142(4):1366-71. [PubMed: 2464645]  [MGI Ref ID J:52612]

Additional References

Newberg MH; Smith DH; Haertel SB; Vining DR; Lacy E; Engelhard VH. 1996. Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules. J Immunol 156(7):2473-80. [PubMed: 8786307]  [MGI Ref ID J:31937]

Tg(HLA-A2.1)1Enge related

Banuelos SJ; Shultz LD; Greiner DL; Burzenski LM; Gott B; Lyons BL; Rossini AA; Appel MC. 2004. Rejection of human islets and human HLA-A2.1 transgenic mouse islets by alloreactive human lymphocytes in immunodeficient NOD-scid and NOD-Rag1(null)Prf1(null) mice. Clin Immunol 112(3):273-83. [PubMed: 15308121]  [MGI Ref ID J:91764]

Billerbeck E; Horwitz JA; Labitt RN; Donovan BM; Vega K; Budell WC; Koo GC; Rice CM; Ploss A. 2013. Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice. J Immunol 191(4):1753-64. [PubMed: 23833235]  [MGI Ref ID J:205706]

Marron MP; Graser RT; Chapman HD; Serreze DV. 2002. Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. Proc Natl Acad Sci U S A 99(21):13753-8. [PubMed: 12361980]  [MGI Ref ID J:109851]

Newberg MH; Smith DH; Haertel SB; Vining DR; Lacy E; Engelhard VH. 1996. Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules. J Immunol 156(7):2473-80. [PubMed: 8786307]  [MGI Ref ID J:31937]

Shultz LD; Ishikawa F; Greiner DL. 2007. Humanized mice in translational biomedical research. Nat Rev Immunol 7(2):118-30. [PubMed: 17259968]  [MGI Ref ID J:142414]

Strowig T; Gurer C; Ploss A; Liu YF; Arrey F; Sashihara J; Koo G; Rice CM; Young JW; Chadburn A; Cohen JI; Munz C. 2009. Priming of protective T cell responses against virus-induced tumors in mice with human immune system components. J Exp Med 206(6):1423-34. [PubMed: 19487422]  [MGI Ref ID J:149509]

Whitfield-Larry F; Young EF; Talmage G; Fudge E; Azam A; Patel S; Largay J; Byrd W; Buse J; Calikoglu AS; Shultz LD; Frelinger JA. 2011. HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/gammac(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells. Diabetes 60(6):1726-33. [PubMed: 21521873]  [MGI Ref ID J:177952]

Yang J; Qian J; Wezeman M; Wang S; Lin P; Wang M; Yaccoby S; Kwak LW; Barlogie B; Yi Q. 2006. Targeting beta2-microglobulin for induction of tumor apoptosis in human hematological malignancies. Cancer Cell 10(4):295-307. [PubMed: 17045207]  [MGI Ref ID J:114975]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX1

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Breeding Considerations This strain is a good breeder.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Weeks of AgePrice per mouse (US dollars $)GenderGenotypes Provided
3 weeks $108.20Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
4 weeks $108.20Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
5 weeks $108.20Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
6 weeks $113.65Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
7 weeks $119.10Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
8 weeks $124.55Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
Price per Pair (US dollars $)Pair Genotype
$227.30Homozygous for Tg(HLA-A2.1)1Enge x Homozygous for Tg(HLA-A2.1)1Enge  

Standard Supply

Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.

Supply Notes

  • Pair Pricing: Price may vary depending on the age of the males and females available for shipment. The price displayed is for a male and female at six weeks of age.
  • Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Weeks of AgePrice per mouse (US dollars $)GenderGenotypes Provided
3 weeks $140.70Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
4 weeks $140.70Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
5 weeks $140.70Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
6 weeks $147.80Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
7 weeks $154.90Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
8 weeks $162.00Female or MaleHomozygous for Tg(HLA-A2.1)1Enge  
Price per Pair (US dollars $)Pair Genotype
$295.50Homozygous for Tg(HLA-A2.1)1Enge x Homozygous for Tg(HLA-A2.1)1Enge  

Standard Supply

Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.

Supply Notes

  • Pair Pricing: Price may vary depending on the age of the males and females available for shipment. The price displayed is for a male and female at six weeks of age.
  • Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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