Strain Name:

C57BL/6-Tg(HLA-A2.1)1Enge/J

Stock Number:

003475

Availability:

Level 4

Use Restrictions Apply, see Terms of Use

Description

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
GenerationN2F28 (20-DEC-06)
 
Donating Investigator Victor Engelhard,   University of Virginia

Appearance
black
Related Genotype: a/a

Description
Homozygous mice carrying the Tg(HLA-A2.1)1Enge transgene express significant quantities of the human class I MHC Ag HLA-A2.1 on cells from the spleen, bone marrow and thymus. Expression of this human class I molecule did not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. These transgenic mice have been used to identify hepatitic C virus (HCV) peptides expressing a sequence for HLA-A2.1 binding that are actually recognized by human A2.1-restricted CTLs. Thus, this transgenic model is important for the study of HLA-restricted CTL determinants and in potential development of a vaccine against HCV. *Note: copy number may be variable. Hemizygotes are tested for expression prior to distribution.

Development
Transgenic mice were constructed by microinjection of the 7-kb EcoRI fragment containing the full length HLA-A2.1 gene into fertilized eggs from C57BL/6 mice.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying   Tg(HLA-A2.1)1Enge     (4 strains)

View Strains carrying other alleles of HLA-A     (5 strains)

Additional Web Information

Genetic Quality Control Annual Report
Technical Bulletin - Monitoring the Expression of the HLA-A2.1 Transgene

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Tg(HLA-A2.1)1Enge/0

        involves: C57BL/6
  • immune system phenotype
  • abnormal immune tolerance (MGI Ref ID J:52612)
    • transgenic animals are more tolerant to HLA-A2.1-expressing murine cells than control mice primed once with influenza virus; numbers of cytotoxic T lymphocyte precursors are 100-fold higher in primed C57BL/6 controls
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

HLA-A related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Vaccine Development

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(HLA-A2.1)1Enge
Allele Name transgene insertion 1, Victor H Engelhard
Allele Type Transgenic (random, expressed)
Mutation Made By Ai-Xuan Le,   Stanford University
Strain of OriginC57BL/6
Expressed Gene HLA-A, major histocompatibility complex, class I, A, human
General Note Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2.
Molecular Note The transgene contains the full length HLA-A2.1 gene. Expression of human class I MHC Ag HLA-A2.1 was detected on cells from homozygous transgenic spleen, bone marrow, and thymus. [MGI Ref ID J:52612]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(HLA-A2.1)1Enge, QPCR, vers. 2
Tg(HLA-A2.1)1Enge, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Le AX; Bernhard EJ; Holterman MJ; Strub S; Parham P; Lacy E; Engelhard VH. 1989. Cytotoxic T cell responses in HLA-A2.1 transgenic mice. Recognition of HLA alloantigens and utilization of HLA-A2.1 as a restriction element. J Immunol 142(4):1366-71. [PubMed: 2464645]  [MGI Ref ID J:52612]

Additional References

Newberg MH; Smith DH; Haertel SB; Vining DR; Lacy E; Engelhard VH. 1996. Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules. J Immunol 156(7):2473-80. [PubMed: 8786307]  [MGI Ref ID J:31937]

Tg(HLA-A2.1)1Enge related

Banuelos SJ; Shultz LD; Greiner DL; Burzenski LM; Gott B; Lyons BL; Rossini AA; Appel MC. 2004. Rejection of human islets and human HLA-A2.1 transgenic mouse islets by alloreactive human lymphocytes in immunodeficient NOD-scid and NOD-Rag1(null)Prf1(null) mice. Clin Immunol 112(3):273-83. [PubMed: 15308121]  [MGI Ref ID J:91764]

Marron MP; Graser RT; Chapman HD; Serreze DV. 2002. Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. Proc Natl Acad Sci U S A 99(21):13753-8. [PubMed: 12361980]  [MGI Ref ID J:109851]

Newberg MH; Smith DH; Haertel SB; Vining DR; Lacy E; Engelhard VH. 1996. Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules. J Immunol 156(7):2473-80. [PubMed: 8786307]  [MGI Ref ID J:31937]

Yang J; Qian J; Wezeman M; Wang S; Lin P; Wang M; Yaccoby S; Kwak LW; Barlogie B; Yi Q. 2006. Targeting beta2-microglobulin for induction of tumor apoptosis in human hematological malignancies. Cancer Cell 10(4):295-307. [PubMed: 17045207]  [MGI Ref ID J:114975]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX8

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*GenderGenotypes Provided
3-5 weeks $66.80Female or MaleHomozygous for Tg(HLA-A2.1)1Enge
6 weeks $70.70Female or MaleHomozygous for Tg(HLA-A2.1)1Enge
7 weeks $74.60Female or MaleHomozygous for Tg(HLA-A2.1)1Enge
8 weeks $78.50Female or MaleHomozygous for Tg(HLA-A2.1)1Enge
Pairs /Price*Pair Genotype
$141.40Homozygous for Tg(HLA-A2.1)1Enge x Homozygous for Tg(HLA-A2.1)1Enge
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*GenderGenotypes Provided
3-5 weeks $86.90Female or MaleHomozygous for Tg(HLA-A2.1)1Enge
6 weeks $92.00Female or MaleHomozygous for Tg(HLA-A2.1)1Enge
7 weeks $97.10Female or MaleHomozygous for Tg(HLA-A2.1)1Enge
8 weeks $102.20Female or MaleHomozygous for Tg(HLA-A2.1)1Enge
Pairs /Price*Pair Genotype
$183.90Homozygous for Tg(HLA-A2.1)1Enge x Homozygous for Tg(HLA-A2.1)1Enge
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Supply Details

Standard SupplyLevel 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.
Supply Notes
  • Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
  • Strains that must be genotyped are not available until five to seven weeks of age.
  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use

Terms of Use


General Terms and Conditions


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Contact information

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fax:207-288-6655

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