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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Breeding Considerations This strain is a good breeder. Species laboratory mouse Generation F13 (24-SEP-12)
Generation DefinitionsDonating Investigator Dr. Victor H. Engelhard, University of Virginia
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Related Genotype: a/aDescription
Homozygous mice carrying the Tg(HLA-A2.1)1Enge transgene express significant quantities of the human class I MHC Ag HLA-A2.1 on cells from the spleen, bone marrow and thymus. Expression of this human class I molecule did not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. These transgenic mice have been used to identify hepatitic C virus (HCV) peptides expressing a sequence for HLA-A2.1 binding that are actually recognized by human A2.1-restricted CTLs. Thus, this transgenic model is important for the study of HLA-restricted CTL determinants and in potential development of a vaccine against HCV.Development
Transgenic mice were constructed by microinjection of the 7-kb EcoRI fragment containing the full length HLA-A2.1 gene into fertilized eggs from C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(HLA-A2.1)1Enge allele
View Strains carrying Tg(HLA-A2.1)1Enge (5 strains)
Additional Web Information
Technical Bulletin - Monitoring the Expression of the HLA-A2.1 Transgene
Phenotype
Phenotype Information
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Major Histocompatibility Complex, Class I, A; HLA-A (HLA-A)
Severe Cutaneous Adverse Reaction, Susceptibility to (HLA-A)
assigned by genotype
Tg(HLA-A2.1)1Enge/0
involves: C57BL/6
- immune system phenotype
- abnormal immune tolerance
- transgenic animals are more tolerant to HLA-A2.1-expressing murine cells than control mice primed once with influenza virus; numbers of cytotoxic T lymphocyte precursors are 100-fold higher in primed C57BL/6 controls (MGI Ref ID J:52612)
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
HLA transgenics
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(HLA-A2.1)1Enge | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Victor H Engelhard | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Dr. Ai-Xuan Le, Stanford University | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human | ||
| Promoter | HLA-A2.1, major histocompatibility complex, class I, subtype A2.1, human | ||
| General Note | Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. | ||
| Molecular Note | The transgene contains the full length HLA-A2.1 gene. Expression of human class I MHC Ag HLA-A2.1 was detected on cells from homozygous transgenic spleen, bone marrow, and thymus. [MGI Ref ID J:52612] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(HLA-A/H2-D), QPCR
Tg(HLA-A2.1)1Enge, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Le AX; Bernhard EJ; Holterman MJ; Strub S; Parham P; Lacy E; Engelhard VH. 1989. Cytotoxic T cell responses in HLA-A2.1 transgenic mice. Recognition of HLA alloantigens and utilization of HLA-A2.1 as a restriction element. J Immunol 142(4):1366-71. [PubMed: 2464645] [MGI Ref ID J:52612]
Additional References
Newberg MH; Smith DH; Haertel SB; Vining DR; Lacy E; Engelhard VH. 1996. Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules. J Immunol 156(7):2473-80. [PubMed: 8786307] [MGI Ref ID J:31937]
Tg(HLA-A2.1)1Enge relatedBanuelos SJ; Shultz LD; Greiner DL; Burzenski LM; Gott B; Lyons BL; Rossini AA; Appel MC. 2004. Rejection of human islets and human HLA-A2.1 transgenic mouse islets by alloreactive human lymphocytes in immunodeficient NOD-scid and NOD-Rag1(null)Prf1(null) mice. Clin Immunol 112(3):273-83. [PubMed: 15308121] [MGI Ref ID J:91764]
Marron MP; Graser RT; Chapman HD; Serreze DV. 2002. Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. Proc Natl Acad Sci U S A 99(21):13753-8. [PubMed: 12361980] [MGI Ref ID J:109851]
Newberg MH; Smith DH; Haertel SB; Vining DR; Lacy E; Engelhard VH. 1996. Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules. J Immunol 156(7):2473-80. [PubMed: 8786307] [MGI Ref ID J:31937]
Shultz LD; Ishikawa F; Greiner DL. 2007. Humanized mice in translational biomedical research. Nat Rev Immunol 7(2):118-30. [PubMed: 17259968] [MGI Ref ID J:142414]
Strowig T; Gurer C; Ploss A; Liu YF; Arrey F; Sashihara J; Koo G; Rice CM; Young JW; Chadburn A; Cohen JI; Munz C. 2009. Priming of protective T cell responses against virus-induced tumors in mice with human immune system components. J Exp Med 206(6):1423-34. [PubMed: 19487422] [MGI Ref ID J:149509]
Whitfield-Larry F; Young EF; Talmage G; Fudge E; Azam A; Patel S; Largay J; Byrd W; Buse J; Calikoglu AS; Shultz LD; Frelinger JA. 2011. HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/gammac(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells. Diabetes 60(6):1726-33. [PubMed: 21521873] [MGI Ref ID J:177952]
Yang J; Qian J; Wezeman M; Wang S; Lin P; Wang M; Yaccoby S; Kwak LW; Barlogie B; Yi Q. 2006. Targeting beta2-microglobulin for induction of tumor apoptosis in human hematological malignancies. Cancer Cell 10(4):295-307. [PubMed: 17045207] [MGI Ref ID J:114975]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX1
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Breeding Considerations This strain is a good breeder.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Weeks of Age Price per mouse (US dollars $) Gender Genotypes Provided 3 weeks $96.20 Female or Male Homozygous for Tg(HLA-A2.1)1Enge 4 weeks $96.20 Female or Male Homozygous for Tg(HLA-A2.1)1Enge 5 weeks $96.20 Female or Male Homozygous for Tg(HLA-A2.1)1Enge 6 weeks $101.20 Female or Male Homozygous for Tg(HLA-A2.1)1Enge 7 weeks $106.20 Female or Male Homozygous for Tg(HLA-A2.1)1Enge 8 weeks $111.20 Female or Male Homozygous for Tg(HLA-A2.1)1Enge
Price per Pair (US dollars $) Pair Genotype $202.40 Homozygous for Tg(HLA-A2.1)1Enge x Homozygous for Tg(HLA-A2.1)1Enge Standard Supply
Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.
Supply Notes
- Pair Pricing: Price may vary depending on the age of the males and females available for shipment. The price displayed is for a male and female at six weeks of age.
- Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
| Pricing for International shipping destinations |
|
Live Mice
Weeks of Age Price per mouse (US dollars $) Gender Genotypes Provided 3 weeks $125.10 Female or Male Homozygous for Tg(HLA-A2.1)1Enge 4 weeks $125.10 Female or Male Homozygous for Tg(HLA-A2.1)1Enge 5 weeks $125.10 Female or Male Homozygous for Tg(HLA-A2.1)1Enge 6 weeks $131.60 Female or Male Homozygous for Tg(HLA-A2.1)1Enge 7 weeks $138.10 Female or Male Homozygous for Tg(HLA-A2.1)1Enge 8 weeks $144.60 Female or Male Homozygous for Tg(HLA-A2.1)1Enge
Price per Pair (US dollars $) Pair Genotype $263.20 Homozygous for Tg(HLA-A2.1)1Enge x Homozygous for Tg(HLA-A2.1)1Enge Standard Supply
Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.
Supply Notes
- Pair Pricing: Price may vary depending on the age of the males and females available for shipment. The price displayed is for a male and female at six weeks of age.
- Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
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Standard Supply
Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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