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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names FVB/NJ-TgN(XGFAPLacZ)3Mes (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation +N1 Donating Investigator IMR Colony, The Jackson Laboratory Appearance
albino
Related Genotype: Tyrc/TyrcImportant Note
This strain is homozygous for the retinal degeneration allele Pde6brd1.Description
Mice carrying this transgene express beta galactosidase in the nuclei of astrocytes. The donating investigator reports that more recent studies on this particular line indicate low level expression of the lacZ reporter gene in many neuronal populations as well. The transgene integrated on the X chromosome and therefore undergoes X-inactivation. This strain may be useful for cell culture and transplantation studies.Development
This strain was developed in the laboratory of Dr. Albee Messing at the University of Wisconsin School of Veterinary Medicine. The construct contains base pairs -2163 to +47 relative to the transcriptional start site of human GFAP, and has the normal ATG start site at +15 converted to TTG by site-directed mutagenesis so that protein synthesis initiates within the lacZ coding region. The construct results in targeting of the enzyme to the nucleus. The transgene was injected into B6SJL/F2 mice and integrated onto the X chromosome.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (178 strains)
004632 B6.Cg-Tg(GFAP-APOE*2)14Hol Apoetm1Unc/J 004633 B6.Cg-Tg(GFAP-APOE*3)37Hol Apoetm1Unc/J 004631 B6.Cg-Tg(GFAP-APOE*4)1Hol Apoetm1Unc/J 005964 B6.Cg-Tg(GFAP-tTA)110Pop/J 004600 FVB-Tg(GFAP-cre)25Mes/J 003257 FVB/N-Tg(GFAPGFP)14Mes/J 006778 NOD/ShiLt-Tg(GFAP-Cd80)9Mdos/MdosJ 003528 STOCK Tg(GFAP-TVA)5Hev/J
004297 B6.CXB1-Pde6brd10/J 002802 C3.BLiA Pde6b+-Krd/J 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004766 C57BL/6J-Pde6brd1-2J/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
lacZ relatedNeurobiology Research
lacZ expression in neural tissue
Astrocyte Defects (astrocyte-specific marker)
Research Tools
lacZ Expression
Genetics Research (Tissue/Cell Markers: astrocyte-specific marker)
Neurobiology Research (astrocyte-specific marker)
Pde6brd1 relatedResearch Tools
lacZ Expression
Mouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(XGFAP-lacZ)3Mes | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, Albee Messing | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg(X;GFAP,lacZ)Mes3; Tg(XGFAPlacZ)3Mes; TgN(XGFAPLacZ)3Mes; line7-2; | ||
| Strain of Origin | C57BL/6J x SJL | ||
| Site of Expression | Beta-galactosidase is expressed in the nuclei of astrocytes. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | GFAP, glial fibrillary acidic protein, human | ||
| General Note | Mice carrying this transgene express beta galactosidase in the nuclei of astrocytes. Transgenic mice exhibit upregulation of GFAP gene activity similar to that which follows injury to the brain. The transgene inserted on the X Chromosome and appears to undergo the usual random inactivation that achieves gene dosage compensation in females. The brains of hemizygous females stain uniformly rather than displaying mosaic patches, suggesting that astrocytes intermingle following their formation. | ||
| Molecular Note | The transgene contains a bacterial lacZ gene linked to a 2.2 kilobase 5'-flanking sequence derived from the human glial fibrillary acidic protein gene. The human GFAP promoter sequence has been shownto direct astrocyte-specific transcription in culturedcells. [MGI Ref ID J:92866] | ||
| Allele Symbol | Pde6brd1 | ||
| Allele Name | retinal degeneration 1 | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | rd; rd-1; rd1; rodless retina; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Brenner M; Kisseberth WC; Su Y; Besnard F; Messing A. 1994. GFAP promoter directs astrocyte-specific expression in transgenic mice. J Neurosci 14(3 Pt 1):1030-7. [PubMed: 8120611] [MGI Ref ID J:92866]
Additional References
Shu T; Richards LJ. 2001. Cortical axon guidance by the glial wedge during the development of the corpus callosum. J Neurosci 21(8):2749-58. [PubMed: 11306627] [MGI Ref ID J:68842]
Tg(XGFAP-lacZ)3Mes relatedRio C; Dikkes P; Liberman MC; Corfas G. 2002. Glial fibrillary acidic protein expression and promoter activity in the inner ear of developing and adult mice. J Comp Neurol 442(2):156-62. [PubMed: 11754168] [MGI Ref ID J:130558]
Shu T; Richards LJ. 2001. Cortical axon guidance by the glial wedge during the development of the corpus callosum. J Neurosci 21(8):2749-58. [PubMed: 11306627] [MGI Ref ID J:68842]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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| Important Note | This strain is homozygous for the retinal degeneration allele Pde6brd1. |
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| fax: | 207-288-6655 |
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