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- Description
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- Genes & alleles
- Genotyping
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Description
Strain Information
Former Names B6;129S4-Gt(ROSA)26Sortm1Sho (Changed: 15-DEC-04 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N4F7 (08-JAN-08) Donating Investigator Stuart Orkin, Harvard Medical School Appearance
black
Related Genotype: a/aDescription
Mice with the Gtrosa26tm1Sho targeted mutation are similar to the B6;129-Gtrosa26tm1Sor from Dr. Philippe Soriano, but reported in the literature to be an improved reporter strain for monitoring cre-mediated excisions. The B-galactosidase-neomycin phosphotransferase fusion gene (Bgeo)-trapped reverse orientation splice acceptor Bgeo line 26 (ROSA26) locus was modified by gene targeting such that Bgeo is expressed only after Cre-mediated excision of loxP-flanked DNA sequences. Bgeo from the excised ROSA26 allele is expressed ubiquitously in embryos and adult mice. When mating the reporter strain with cre-expressing transgenic mice, one can see that the loxP-flanked ROSA26 allele is accessible to cre during early embryogenesis, as well as in a specific hematopoietic lineage (T lymphocytes). These mice may prove useful in the study of cell fate and cell migration during embryogenesis through recombinase-activated tagging. Homozygotes are viable and fertile.Development
The investigator modified the ROSA26 gene trap locus to engineer a reporter mouse strain suitable for in vivo assay of the function of Cre, which might be delivered as a transgene, a virus, or by microinjection. It harbors a single-copy reporter gene, eliminating potential problems such as chromosome loss associated with Cre-mediated recombination of multicopy floxed alleles. 129S4/SvJaeSor derived ROSA26 (AK7) embryonic stem (ES) cells were used.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (178 strains)
Additional Web Information
Cre-lox Systems
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Bgeo relatedNeurobiology Research
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Research Tools
lacZ Expression
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System: ubiquitously expressed lox-STOP-lox-LacZ transgene)
Genetics Research (Tissue/Cell Markers: Cre-lox System: ubiquitously expressed lox-STOP-lox-LacZ transgene)
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1Sho | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Stuart Orkin | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | R26-beta-galfl-STOP; R26R; R26Rosa-lox-Stop-lox-LacZ; R26RstoplacZ; ROSA26-stop-lacZ; Rosa26fsLz; gtROSA; | ||
| Mutation Made By | Xiaohong Mao, Novartis | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | when crossed to a cre recombinase-expressing strain, lacZ expression is observed in the cre-expressing tissues; this is similar to Gt(ROSA)26Sortm1Sor but has one copy of the loxP-flanked reporter gene | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| General Note | Mice carrying this mutation are considered to be reporter strains, with successful Cre excision indicated by beta-gal expression in Cre-expressing tissues. | ||
| Molecular Note | A targeting vector was designed to insert floxed (flanked by loxP) phosphoglycerate kinase-1-cytosine deaminase/phosphoglycerate kinase-1-puromycin (PGK-CD/PGK-Puro) and stop sequences between the splicing acceptor (SA) and the beta-gal-neomycin phosphotransferase fusion gene (beta-geo) sequences of the proviral ROSA 26 locus. Correctly targeted, this resulted in ES cells that were Puro resistant but G418 susceptible, and did not express beta-gal. [MGI Ref ID J:64291] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sor, Gt(ROSA)26Sortm1Sho, MCA, vers. 2
Gt(ROSA)26Sor, Gt(ROSA)26Sortm1Sho, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Mao X; Fujiwara Y; Orkin SH. 1999. Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc Natl Acad Sci U S A 96(9):5037-42. [PubMed: 10220414] [MGI Ref ID J:64291]
Additional References
Egan LJ; Eckmann L; Greten FR; Chae S; Li ZW; Myhre GM; Robine S; Karin M; Kagnoff MF. 2004. IkappaB-kinasebeta-dependent NF-kappaB activation provides radioprotection to the intestinal epithelium. Proc Natl Acad Sci U S A 101(8):2452-7. [PubMed: 14983030] [MGI Ref ID J:88644]
Xin HB; Deng KY; Rishniw M; Ji G; Kotlikoff MI. 2002. Smooth muscle expression of Cre recombinase and eGFP in transgenic mice. Physiol Genomics 10(3):211-5. [PubMed: 12209023] [MGI Ref ID J:81358]
Gt(ROSA)26Sortm1Sho relatedBerger J; Eckert S; Scardigli R; Guillemot F; Gruss P; Stoykova A. 2004. E1-Ngn2/Cre is a new line for regional activation of Cre recombinase in the developing CNS. Genesis 40(4):195-9. [PubMed: 15593326] [MGI Ref ID J:95642]
Casola S; Cattoretti G; Uyttersprot N; Koralov SB; Segal J; Hao Z; Waisman A; Egert A; Ghitza D; Rajewsky K. 2006. Tracking germinal center B cells expressing germ-line immunoglobulin gamma1 transcripts by conditional gene targeting. Proc Natl Acad Sci U S A 103(19):7396-401. [PubMed: 16651521] [MGI Ref ID J:109583]
Egawa T; Eberl G; Taniuchi I; Benlagha K; Geissmann F; Hennighausen L; Bendelac A; Littman DR. 2005. Genetic evidence supporting selection of the valpha14i NKT cell lineage from double-positive thymocyte precursors. Immunity 22(6):705-16. [PubMed: 15963785] [MGI Ref ID J:99111]
Garrick D; Sharpe JA; Arkell R; Dobbie L; Smith AJ; Wood WG; Higgs DR; Gibbons RJ. 2006. Loss of Atrx affects trophoblast development and the pattern of X-inactivation in extraembryonic tissues. PLoS Genet 2(4):e58. [PubMed: 16628246] [MGI Ref ID J:115836]
Hafner M; Wenk J; Nenci A; Pasparakis M; Scharffetter-Kochanek K; Smyth N; Peters T; Kess D; Holtkotter O; Shephard P; Kudlow JE; Smola H; Haase I; Schippers A; Krieg T; Muller W. 2004. Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein. Genesis 38(4):176-81. [PubMed: 15083518] [MGI Ref ID J:89717]
Li Z; Chen MJ; Stacy T; Speck NA. 2006. Runx1 function in hematopoiesis is required in cells that express Tek. Blood 107(1):106-10. [PubMed: 16174759] [MGI Ref ID J:125796]
Lu TL; Chang JL; Liang CC; You LR; Chen CM. 2007. Tumor spectrum, tumor latency and tumor incidence of the pten-deficient mice. PLoS ONE 2(11):e1237. [PubMed: 18043744] [MGI Ref ID J:130367]
Peng X; Wu X; Druso JE; Wei H; Park AY; Kraus MS; Alcaraz A; Chen J; Chien S; Cerione RA; Guan JL. 2008. Cardiac developmental defects and eccentric right ventricular hypertrophy in cardiomyocyte focal adhesion kinase (FAK) conditional knockout mice. Proc Natl Acad Sci U S A 105(18):6638-43. [PubMed: 18448675] [MGI Ref ID J:134635]
Pratt T; Tian NM; Simpson TI; Mason JO; Price DJ. 2004. The winged helix transcription factor Foxg1 facilitates retinal ganglion cell axon crossing of the ventral midline in the mouse. Development 131(15):3773-84. [PubMed: 15240555] [MGI Ref ID J:92062]
Ramirez A; Page A; Gandarillas A; Zanet J; Pibre S; Vidal M; Tusell L; Genesca A; Whitaker DA; Melton DW; Jorcano JL. 2004. A keratin K5Cre transgenic line appropriate for tissue-specific or generalized Cre-mediated recombination. Genesis 39(1):52-7. [PubMed: 15124227] [MGI Ref ID J:89783]
Rivera-Feliciano J; Lee KH; Kong SW; Rajagopal S; Ma Q; Springer Z; Izumo S; Tabin CJ; Pu WT. 2006. Development of heart valves requires Gata4 expression in endothelial-derived cells. Development 133(18):3607-18. [PubMed: 16914500] [MGI Ref ID J:112458]
Schwander M; Leu M; Stumm M; Dorchies OM; Ruegg UT; Schittny J; Muller U. 2003. Beta1 integrins regulate myoblast fusion and sarcomere assembly. Dev Cell 4(5):673-85. [PubMed: 12737803] [MGI Ref ID J:129360]
Scott MM; Wylie CJ; Lerch JK; Murphy R; Lobur K; Herlitze S; Jiang W; Conlon RA; Strowbridge BW; Deneris ES. 2005. A genetic approach to access serotonin neurons for in vivo and in vitro studies. Proc Natl Acad Sci U S A 102(45):16472-7. [PubMed: 16251278] [MGI Ref ID J:103436]
Sgaier SK; Millet S; Villanueva MP; Berenshteyn F; Song C; Joyner AL. 2005. Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping. Neuron 45(1):27-40. [PubMed: 15629700] [MGI Ref ID J:95823]
Stenman J; Toresson H; Campbell K. 2003. Identification of two distinct progenitor populations in the lateral ganglionic eminence: implications for striatal and olfactory bulb neurogenesis. J Neurosci 23(1):167-74. [PubMed: 12514213] [MGI Ref ID J:82693]
Wrobel CN; Mutch CA; Swaminathan S; Taketo MM; Chenn A. 2007. Persistent expression of stabilized beta-catenin delays maturation of radial glial cells into intermediate progenitors. Dev Biol 309(2):285-97. [PubMed: 17706960] [MGI Ref ID J:124867]
Zadelaar SM; Boesten LS; Pires NM; van Nieuwkoop A; Biessen EA; Jukema W; Havekes LM; van Vlijmen BJ; Willems van Dijk K. 2006. Local cre-mediated gene recombination in vascular smooth muscle cells in mice. Transgenic Res 15(1):31-6. [PubMed: 16475008] [MGI Ref ID J:106456]
Zeisberg EM; Ma Q; Juraszek AL; Moses K; Schwartz RJ; Izumo S; Pu WT. 2005. Morphogenesis of the right ventricle requires myocardial expression of Gata4. J Clin Invest 115(6):1522-1531. [PubMed: 15902305] [MGI Ref ID J:99203]
Zeisberg EM; Potenta S; Xie L; Zeisberg M; Kalluri R. 2007. Discovery of endothelial to mesenchymal transition as a source for carcinoma-associated fibroblasts. Cancer Res 67(21):10123-8. [PubMed: 17974953] [MGI Ref ID J:127148]
Zhang DJ; Wang Q; Wei J; Baimukanova G; Buchholz F; Stewart AF; Mao X; Killeen N. 2005. Selective expression of the Cre recombinase in late-stage thymocytes using the distal promoter of the Lck gene. J Immunol 174(11):6725-31. [PubMed: 15905512] [MGI Ref ID J:99042]
Zhou B; Ma Q; Rajagopal S; Wu SM; Domian I; Rivera-Feliciano J; Jiang D; von Gise A; Ikeda S; Chien KR; Pu WT. 2008. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454(7200):109-13. [PubMed: 18568026] [MGI Ref ID J:137426]
Zhuang X; Masson J; Gingrich JA; Rayport S; Hen R. 2005. Targeted gene expression in dopamine and serotonin neurons of the mouse brain. J Neurosci Methods 143(1):27-32. [PubMed: 15763133] [MGI Ref ID J:115426]
de Alboran IM; Baena E; Martinez-A C. 2004. c-Myc-deficient B lymphocytes are resistant to spontaneous and induced cell death. Cell Death Differ 11(1):61-8. [PubMed: 12970677] [MGI Ref ID J:115648]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $152.60 Female or Male Homozygous for Gt(ROSA)26Sortm1Sho *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $305.20 Homozygous for Gt(ROSA)26Sortm1Sho x Homozygous for Gt(ROSA)26Sortm1Sho
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $198.40 Female or Male Homozygous for Gt(ROSA)26Sortm1Sho *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $396.80 Homozygous for Gt(ROSA)26Sortm1Sho x Homozygous for Gt(ROSA)26Sortm1Sho
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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