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| The β-galactosidase-neomycin phosphotransferase fusion gene (βgeo)-trapped reverse orientation splice acceptor βgeo line 26 (ROSA26) locus was modified by gene targeting such that βgeo is expressed only after Cre-mediated excision of loxP-flanked DNA sequences. These mice may prove useful in the study of cell fate and cell migration during embryogenesis through recombinase-activated tagging. | |||||||||||||||
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;129S4-Gt(ROSA)26Sortm1Sho (Changed: 15-DEC-04 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N3F8pN1
Generation DefinitionsDonating Investigator Stuart Orkin, Harvard Medical School Appearance
black
Related Genotype: a/aImportant Note
It has been the experience of The Jackson Laboratory that lacZ expression diminishes postnatally in these mice (Stock No. 003504). It is our recommendation that this strain be used for studying embryonic time points. For ubiquitous lacZ reporter activity through adulthood, we recommend Gt(ROSA)26Sortm1Sor mice on a C57BL/6 congenic background (Stock No. 003474), B6;129 genetic background (Stock No. 003309), 129 genetic background (Stock No. 003310), or FVB congenic background (Stock No. 009427).Description
Mice with the Gt(ROSA)26tm1Sho targeted mutation are similar to the B6;129-Gt(ROSA)26tm1Sor from Dr. Philippe Soriano, but reported in the literature to be an improved reporter strain for monitoring cre-mediated excisions (see Important Note). The β-galactosidase-neomycin phosphotransferase fusion gene (βgeo)-trapped reverse orientation splice acceptor βgeo line 26 (ROSA26) locus was modified by gene targeting such that βgeo is expressed only after Cre-mediated excision of loxP-flanked DNA sequences. βgeo from the excised ROSA26 allele is expressed ubiquitously in embryos and adult mice. When mating the reporter strain with cre-expressing transgenic mice, one can see that the loxP-flanked ROSA26 allele is accessible to cre during early embryogenesis, as well as in a specific hematopoietic lineage (T lymphocytes). These mice may prove useful in the study of cell fate and cell migration during embryogenesis through recombinase-activated tagging. Homozygotes are viable and fertile.Development
The investigator modified the ROSA26 gene trap locus to engineer a reporter mouse strain suitable for in vivo assay of the function of Cre recombinase, which might be delivered as a transgene, a virus, or by microinjection. It harbors a single-copy reporter gene, eliminating potential problems such as chromosome loss associated with Cre-mediated recombination of multicopy floxed alleles. 129S4/SvJaeSor derived ROSA26 (AK7) embryonic stem (ES) cells were used.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (245 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Research Tools
lacZ Expression
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Mutagenesis and Transgenesis: Cre-lox System: ubiquitously expressed lox-STOP-lox-LacZ transgene
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System: ubiquitously expressed lox-STOP-lox-LacZ transgene
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1Sho | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Stuart Orkin | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | R26-beta-galfl-STOP; R26R; R26Rosa-lox-Stop-lox-LacZ; R26RstoplacZ; ROSA26-loxP-stoploxP-beta-geo; ROSA26-stop-lacZ; ROSA26R-LacZ reporter; Rosa26fsLz; gtROSA; | ||
| Mutation Made By | Xiaohong Mao, Novartis | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | when crossed to a cre recombinase-expressing strain, lacZ expression is observed in the cre-expressing tissues; this is similar to Gt(ROSA)26Sortm1Sor but has one copy of the loxP-flanked reporter gene | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| General Note | Mice carrying this mutation are considered to be reporter strains, with successful Cre excision indicated by beta-gal expression in Cre-expressing tissues. | ||
| Molecular Note | A targeting vector was designed to insert floxed (flanked by loxP) phosphoglycerate kinase-1-cytosine deaminase/phosphoglycerate kinase-1-puromycin (PGK-CD/PGK-Puro) and stop sequences between the splicing acceptor (SA) and the beta-gal-neomycin phosphotransferase fusion gene (beta-geo) sequences of the proviral ROSA 26 locus. Correctly targeted, this resulted in ES cells that were Puro resistant but G418 susceptible, and did not express beta-gal. [MGI Ref ID J:64291] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sor MCA, Melt Curve Analysis
Gt(ROSA)26Sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Mao X; Fujiwara Y; Orkin SH. 1999. Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc Natl Acad Sci U S A 96(9):5037-42. [PubMed: 10220414] [MGI Ref ID J:64291]
Additional References
Egan LJ; Eckmann L; Greten FR; Chae S; Li ZW; Myhre GM; Robine S; Karin M; Kagnoff MF. 2004. IkappaB-kinasebeta-dependent NF-kappaB activation provides radioprotection to the intestinal epithelium. Proc Natl Acad Sci U S A 101(8):2452-7. [PubMed: 14983030] [MGI Ref ID J:88644]
Xin HB; Deng KY; Rishniw M; Ji G; Kotlikoff MI. 2002. Smooth muscle expression of Cre recombinase and eGFP in transgenic mice. Physiol Genomics 10(3):211-5. [PubMed: 12209023] [MGI Ref ID J:81358]
Gt(ROSA)26Sortm1Sho relatedBenhamouche S; Curto M; Saotome I; Gladden AB; Liu CH; Giovannini M; McClatchey AI. 2010. Nf2/Merlin controls progenitor homeostasis and tumorigenesis in the liver. Genes Dev 24(16):1718-30. [PubMed: 20675406] [MGI Ref ID J:163257]
Berger J; Eckert S; Scardigli R; Guillemot F; Gruss P; Stoykova A. 2004. E1-Ngn2/Cre is a new line for regional activation of Cre recombinase in the developing CNS. Genesis 40(4):195-9. [PubMed: 15593326] [MGI Ref ID J:95642]
Blaess S; Graus-Porta D; Belvindrah R; Radakovits R; Pons S; Littlewood-Evans A; Senften M; Guo H; Li Y; Miner JH; Reichardt LF; Muller U. 2004. Beta1-integrins are critical for cerebellar granule cell precursor proliferation. J Neurosci 24(13):3402-12. [PubMed: 15056720] [MGI Ref ID J:96910]
Bouldin CM; Gritli-Linde A; Ahn S; Harfe BD. 2010. Shh pathway activation is present and required within the vertebrate limb bud apical ectodermal ridge for normal autopod patterning. Proc Natl Acad Sci U S A 107(12):5489-94. [PubMed: 20212115] [MGI Ref ID J:158689]
Braren R; Hu H; Kim YH; Beggs HE; Reichardt LF; Wang R. 2006. Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation. J Cell Biol 172(1):151-62. [PubMed: 16391003] [MGI Ref ID J:104401]
Carlen M; Meletis K; Goritz C; Darsalia V; Evergren E; Tanigaki K; Amendola M; Barnabe-Heider F; Yeung MS; Naldini L; Honjo T; Kokaia Z; Shupliakov O; Cassidy RM; Lindvall O; Frisen J. 2009. Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke. Nat Neurosci 12(3):259-67. [PubMed: 19234458] [MGI Ref ID J:150542]
Casola S; Cattoretti G; Uyttersprot N; Koralov SB; Segal J; Hao Z; Waisman A; Egert A; Ghitza D; Rajewsky K. 2006. Tracking germinal center B cells expressing germ-line immunoglobulin gamma1 transcripts by conditional gene targeting. Proc Natl Acad Sci U S A 103(19):7396-401. [PubMed: 16651521] [MGI Ref ID J:109583]
Chen MJ; Yokomizo T; Zeigler BM; Dzierzak E; Speck NA. 2009. Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter. Nature 457(7231):887-91. [PubMed: 19129762] [MGI Ref ID J:145700]
Dy P; Smits P; Silvester A; Penzo-Mendez A; Dumitriu B; Han Y; de la Motte CA; Kingsley DM; Lefebvre V. 2010. Synovial joint morphogenesis requires the chondrogenic action of Sox5 and Sox6 in growth plate and articular cartilage. Dev Biol 341(2):346-59. [PubMed: 20206616] [MGI Ref ID J:160493]
Egawa T; Eberl G; Taniuchi I; Benlagha K; Geissmann F; Hennighausen L; Bendelac A; Littman DR. 2005. Genetic evidence supporting selection of the valpha14i NKT cell lineage from double-positive thymocyte precursors. Immunity 22(6):705-16. [PubMed: 15963785] [MGI Ref ID J:99111]
Feng W; Simoes-de-Souza F; Finger TE; Restrepo D; Williams T. 2009. Disorganized olfactory bulb lamination in mice deficient for transcription factor AP-2epsilon. Mol Cell Neurosci 42(3):161-71. [PubMed: 19580868] [MGI Ref ID J:154236]
Flier SN; Tanjore H; Kokkotou EG; Sugimoto H; Zeisberg M; Kalluri R. 2010. Identification of epithelial to mesenchymal transition as a novel source of fibroblasts in intestinal fibrosis. J Biol Chem 285(26):20202-12. [PubMed: 20363741] [MGI Ref ID J:164559]
Garrick D; Sharpe JA; Arkell R; Dobbie L; Smith AJ; Wood WG; Higgs DR; Gibbons RJ. 2006. Loss of Atrx affects trophoblast development and the pattern of X-inactivation in extraembryonic tissues. PLoS Genet 2(4):e58. [PubMed: 16628246] [MGI Ref ID J:115836]
Gong S; Doughty M; Harbaugh CR; Cummins A; Hatten ME; Heintz N; Gerfen CR. 2007. Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs. J Neurosci 27(37):9817-23. [PubMed: 17855595] [MGI Ref ID J:145110]
Graus-Porta D; Blaess S; Senften M; Littlewood-Evans A; Damsky C; Huang Z; Orban P; Klein R; Schittny JC; Muller U. 2001. Beta1-class integrins regulate the development of laminae and folia in the cerebral and cerebellar cortex. Neuron 31(3):367-79. [PubMed: 11516395] [MGI Ref ID J:71122]
Gurley SB; Riquier-Brison AD; Schnermann J; Sparks MA; Allen AM; Haase VH; Snouwaert JN; Le TH; McDonough AA; Koller BH; Coffman TM. 2011. AT1A angiotensin receptors in the renal proximal tubule regulate blood pressure. Cell Metab 13(4):469-75. [PubMed: 21459331] [MGI Ref ID J:172240]
Hafner M; Wenk J; Nenci A; Pasparakis M; Scharffetter-Kochanek K; Smyth N; Peters T; Kess D; Holtkotter O; Shephard P; Kudlow JE; Smola H; Haase I; Schippers A; Krieg T; Muller W. 2004. Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein. Genesis 38(4):176-81. [PubMed: 15083518] [MGI Ref ID J:89717]
Haldar M; Hedberg ML; Hockin MF; Capecchi MR. 2009. A CreER-based random induction strategy for modeling translocation-associated sarcomas in mice. Cancer Res 69(8):3657-64. [PubMed: 19351831] [MGI Ref ID J:147728]
Hayashi Y; Call MK; Liu CY; Hayashi M; Babcock G; Ohashi Y; Kao WW. 2010. Monoallelic expression of Krt12 gene during corneal-type epithelium differentiation of limbal stem cells. Invest Ophthalmol Vis Sci 51(9):4562-8. [PubMed: 20393120] [MGI Ref ID J:164088]
Huh SH; Ornitz DM. 2010. Beta-catenin deficiency causes DiGeorge syndrome-like phenotypes through regulation of Tbx1. Development 137(7):1137-47. [PubMed: 20215350] [MGI Ref ID J:158677]
Ichise T; Yoshida N; Ichise H. 2010. H-, N- and Kras cooperatively regulate lymphatic vessel growth by modulating VEGFR3 expression in lymphatic endothelial cells in mice. Development 137(6):1003-13. [PubMed: 20179099] [MGI Ref ID J:159024]
Ivaniutsin U; Chen Y; Mason JO; Price DJ; Pratt T. 2009. Adenomatous polyposis coli is required for early events in the normal growth and differentiation of the developing cerebral cortex. Neural Dev 4:3. [PubMed: 19149881] [MGI Ref ID J:160733]
Leneuve P; Colnot S; Hamard G; Francis F; Niwa-Kawakita M; Giovannini M; Holzenberger M. 2003. Cre-mediated germline mosaicism: a new transgenic mouse for the selective removal of residual markers from tri-lox conditional alleles. Nucleic Acids Res 31(5):e21. [PubMed: 12595570] [MGI Ref ID J:94551]
Li Z; Chen MJ; Stacy T; Speck NA. 2006. Runx1 function in hematopoiesis is required in cells that express Tek. Blood 107(1):106-10. [PubMed: 16174759] [MGI Ref ID J:125796]
Li Z; Tognon CE; Godinho FJ; Yasaitis L; Hock H; Herschkowitz JI; Lannon CL; Cho E; Kim SJ; Bronson RT; Perou CM; Sorensen PH; Orkin SH. 2007. ETV6-NTRK3 fusion oncogene initiates breast cancer from committed mammary progenitors via activation of AP1 complex. Cancer Cell 12(6):542-58. [PubMed: 18068631] [MGI Ref ID J:130323]
Lobsiger CS; Boillee S; McAlonis-Downes M; Khan AM; Feltri ML; Yamanaka K; Cleveland DW. 2009. Schwann cells expressing dismutase active mutant SOD1 unexpectedly slow disease progression in ALS mice. Proc Natl Acad Sci U S A 106(11):4465-70. [PubMed: 19251638] [MGI Ref ID J:146766]
Lowe LA; Yamada S; Kuehn MR. 2000. HoxB6-Cre transgenic mice express Cre recombinase in extra-embryonic mesoderm, in lateral plate and limb mesoderm and at the midbrain/hindbrain junction. Genesis 26(2):118-20. [PubMed: 10686603] [MGI Ref ID J:67901]
Lu TL; Chang JL; Liang CC; You LR; Chen CM. 2007. Tumor spectrum, tumor latency and tumor incidence of the pten-deficient mice. PLoS ONE 2(11):e1237. [PubMed: 18043744] [MGI Ref ID J:130367]
Ma Q; Zhou B; Pu WT. 2008. Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity. Dev Biol 323(1):98-104. [PubMed: 18775691] [MGI Ref ID J:141152]
Maska EL; Cserjesi P; Hua LL; Garstka ME; Brody HM; Morikawa Y. 2010. A Tlx2-Cre mouse line uncovers essential roles for Hand1 in extraembryonic and lateral mesoderm. Genesis :. [PubMed: 20506548] [MGI Ref ID J:160214]
Matsuoka T; Ahlberg PE; Kessaris N; Iannarelli P; Dennehy U; Richardson WD; McMahon AP; Koentges G. 2005. Neural crest origins of the neck and shoulder. Nature 436(7049):347-55. [PubMed: 16034409] [MGI Ref ID J:99989]
Miclea RL; Karperien M; Bosch CA; van der Horst G; van der Valk MA; Kobayashi T; Kronenberg HM; Rawadi G; Akcakaya P; Lowik CW; Fodde R; Wit JM; Robanus-Maandag EC. 2009. Adenomatous polyposis coli-mediated control of beta-catenin is essential for both chondrogenic and osteogenic differentiation of skeletal precursors. BMC Dev Biol 9:26. [PubMed: 19356224] [MGI Ref ID J:149225]
Morikawa Y; Cserjesi P. 2008. Cardiac neural crest expression of Hand2 regulates outflow and second heart field development. Circ Res 103(12):1422-9. [PubMed: 19008477] [MGI Ref ID J:155265]
Morris ZS; McClatchey AI. 2009. Aberrant epithelial morphology and persistent epidermal growth factor receptor signaling in a mouse model of renal carcinoma. Proc Natl Acad Sci U S A 106(24):9767-72. [PubMed: 19487675] [MGI Ref ID J:150071]
Nakajima M; Watanabe S; Okuyama S; Shen J; Furukawa Y. 2009. Restricted growth and insulin-like growth factor-1 deficiency in mice lacking presenilin-1 in the neural crest cell lineage. Int J Dev Neurosci 27(8):837-43. [PubMed: 19665542] [MGI Ref ID J:157272]
Narboux-Neme N; Pavone LM; Avallone L; Zhuang X; Gaspar P. 2008. Serotonin transporter transgenic (SERTcre) mouse line reveals developmental targets of serotonin specific reuptake inhibitors (SSRIs). Neuropharmacology 55(6):994-1005. [PubMed: 18789954] [MGI Ref ID J:142523]
Pan W; Jin Y; Stanger B; Kiernan AE. 2010. Notch signaling is required for the generation of hair cells and supporting cells in the mammalian inner ear. Proc Natl Acad Sci U S A 107(36):15798-803. [PubMed: 20733081] [MGI Ref ID J:164383]
Pasparakis M; Courtois G; Hafner M; Schmidt-Supprian M; Nenci A; Toksoy A; Krampert M; Goebeler M; Gillitzer R; Israel A; Krieg T; Rajewsky K; Haase I. 2002. TNF-mediated inflammatory skin disease in mice with epidermis-specific deletion of IKK2. Nature 417(6891):861-6. [PubMed: 12075355] [MGI Ref ID J:77233]
Peng X; Wu X; Druso JE; Wei H; Park AY; Kraus MS; Alcaraz A; Chen J; Chien S; Cerione RA; Guan JL. 2008. Cardiac developmental defects and eccentric right ventricular hypertrophy in cardiomyocyte focal adhesion kinase (FAK) conditional knockout mice. Proc Natl Acad Sci U S A 105(18):6638-43. [PubMed: 18448675] [MGI Ref ID J:134635]
Pratt T; Tian NM; Simpson TI; Mason JO; Price DJ. 2004. The winged helix transcription factor Foxg1 facilitates retinal ganglion cell axon crossing of the ventral midline in the mouse. Development 131(15):3773-84. [PubMed: 15240555] [MGI Ref ID J:92062]
Ramirez A; Page A; Gandarillas A; Zanet J; Pibre S; Vidal M; Tusell L; Genesca A; Whitaker DA; Melton DW; Jorcano JL. 2004. A keratin K5Cre transgenic line appropriate for tissue-specific or generalized Cre-mediated recombination. Genesis 39(1):52-7. [PubMed: 15124227] [MGI Ref ID J:89783]
Rivera-Feliciano J; Lee KH; Kong SW; Rajagopal S; Ma Q; Springer Z; Izumo S; Tabin CJ; Pu WT. 2006. Development of heart valves requires Gata4 expression in endothelial-derived cells. Development 133(18):3607-18. [PubMed: 16914500] [MGI Ref ID J:112458]
Schwander M; Leu M; Stumm M; Dorchies OM; Ruegg UT; Schittny J; Muller U. 2003. Beta1 integrins regulate myoblast fusion and sarcomere assembly. Dev Cell 4(5):673-85. [PubMed: 12737803] [MGI Ref ID J:129360]
Schwander M; Shirasaki R; Pfaff SL; Muller U. 2004. Beta1 integrins in muscle, but not in motor neurons, are required for skeletal muscle innervation. J Neurosci 24(37):8181-91. [PubMed: 15371519] [MGI Ref ID J:146417]
Stenman J; Toresson H; Campbell K. 2003. Identification of two distinct progenitor populations in the lateral ganglionic eminence: implications for striatal and olfactory bulb neurogenesis. J Neurosci 23(1):167-74. [PubMed: 12514213] [MGI Ref ID J:82693]
Stenman J; Yu RT; Evans RM; Campbell K. 2003. Tlx and Pax6 co-operate genetically to establish the pallio-subpallial boundary in the embryonic mouse telencephalon. Development 130(6):1113-22. [PubMed: 12571103] [MGI Ref ID J:81762]
Stenman JM; Rajagopal J; Carroll TJ; Ishibashi M; McMahon J; McMahon AP. 2008. Canonical Wnt signaling regulates organ-specific assembly and differentiation of CNS vasculature. Science 322(5905):1247-50. [PubMed: 19023080] [MGI Ref ID J:142352]
Vitelli F; Huynh T; Baldini A. 2009. Gain of function of Tbx1 affects pharyngeal and heart development in the mouse. Genesis 47(3):188-95. [PubMed: 19253341] [MGI Ref ID J:146886]
Wong SY; Reiter JF. 2011. From the Cover: Wounding mobilizes hair follicle stem cells to form tumors. Proc Natl Acad Sci U S A 108(10):4093-8. [PubMed: 21321207] [MGI Ref ID J:170482]
Wrobel CN; Mutch CA; Swaminathan S; Taketo MM; Chenn A. 2007. Persistent expression of stabilized beta-catenin delays maturation of radial glial cells into intermediate progenitors. Dev Biol 309(2):285-97. [PubMed: 17706960] [MGI Ref ID J:124867]
Yoshioka R; Shiraishi A; Kobayashi T; Morita S; Hayashi Y; Higashiyama S; Ohashi Y. 2010. Corneal epithelial wound healing impaired in keratinocyte-specific HB-EGF-deficient mice in vivo and in vitro. Invest Ophthalmol Vis Sci 51(11):5630-9. [PubMed: 20554614] [MGI Ref ID J:171398]
Zadelaar SM; Boesten LS; Pires NM; van Nieuwkoop A; Biessen EA; Jukema W; Havekes LM; van Vlijmen BJ; Willems van Dijk K. 2006. Local cre-mediated gene recombination in vascular smooth muscle cells in mice. Transgenic Res 15(1):31-6. [PubMed: 16475008] [MGI Ref ID J:106456]
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Zeisberg EM; Ma Q; Juraszek AL; Moses K; Schwartz RJ; Izumo S; Pu WT. 2005. Morphogenesis of the right ventricle requires myocardial expression of Gata4. J Clin Invest 115(6):1522-1531. [PubMed: 15902305] [MGI Ref ID J:99203]
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Zhang DJ; Wang Q; Wei J; Baimukanova G; Buchholz F; Stewart AF; Mao X; Killeen N. 2005. Selective expression of the Cre recombinase in late-stage thymocytes using the distal promoter of the Lck gene. J Immunol 174(11):6725-31. [PubMed: 15905512] [MGI Ref ID J:99042]
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Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Important Note
It has been the experience of The Jackson Laboratory that lacZ expression diminishes postnatally in these mice (Stock No. 003504). It is our recommendation that this strain be used for studying embryonic time points. For ubiquitous lacZ reporter activity through adulthood, we recommend Gt(ROSA)26Sortm1Sor mice on a C57BL/6 congenic background (Stock No. 003474), B6;129 genetic background (Stock No. 003309), 129 genetic background (Stock No. 003310), or FVB congenic background (Stock No. 009427).
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.