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Description
Strain Information
Former Names B6;129S4-Gt(ROSA)26Sortm1Sho (Changed: 15-DEC-04 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N4F7 (08-JAN-08) Donating Investigator Stuart Orkin, Harvard Medical School Appearance
black
Related Genotype: a/aDescription
Mice with the Gtrosa26tm1Sho targeted mutation are similar to the B6;129-Gtrosa26tm1Sor from Dr. Philippe Soriano, but reported in the literature to be an improved reporter strain for monitoring cre-mediated excisions. The B-galactosidase-neomycin phosphotransferase fusion gene (Bgeo)-trapped reverse orientation splice acceptor Bgeo line 26 (ROSA26) locus was modified by gene targeting such that Bgeo is expressed only after Cre-mediated excision of loxP-flanked DNA sequences. Bgeo from the excised ROSA26 allele is expressed ubiquitously in embryos and adult mice. When mating the reporter strain with cre-expressing transgenic mice, one can see that the loxP-flanked ROSA26 allele is accessible to cre during early embryogenesis, as well as in a specific hematopoietic lineage (T lymphocytes). These mice may prove useful in the study of cell fate and cell migration during embryogenesis through recombinase-activated tagging. Homozygotes are viable and fertile.Development
The investigator modified the ROSA26 gene trap locus to engineer a reporter mouse strain suitable for in vivo assay of the function of Cre, which might be delivered as a transgene, a virus, or by microinjection. It harbors a single-copy reporter gene, eliminating potential problems such as chromosome loss associated with Cre-mediated recombination of multicopy floxed alleles. 129S4/SvJaeSor derived ROSA26 (AK7) embryonic stem (ES) cells were used.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Bgeo relatedNeurobiology Research
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Research Tools
lacZ Expression
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Mutagenesis and Transgenesis: Cre-lox System: ubiquitously expressed lox-STOP-lox-LacZ transgene
Tissue/Cell Markers: Cre-lox System: ubiquitously expressed lox-STOP-lox-LacZ transgene
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1Sho | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Stuart Orkin | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | R26-beta-galfl-STOP; R26R; R26Rosa-lox-Stop-lox-LacZ; R26RstoplacZ; ROSA26-stop-lacZ; Rosa26fsLz; gtROSA; | ||
| Mutation Made By | Xiaohong Mao, Novartis | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | when crossed to a cre recombinase-expressing strain, lacZ expression is observed in the cre-expressing tissues; this is similar to Gt(ROSA)26Sortm1Sor but has one copy of the loxP-flanked reporter gene | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| General Note | Mice carrying this mutation are considered to be reporter strains, with successful Cre excision indicated by beta-gal expression in Cre-expressing tissues. | ||
| Molecular Note | A targeting vector was designed to insert floxed (flanked by loxP) phosphoglycerate kinase-1-cytosine deaminase/phosphoglycerate kinase-1-puromycin (PGK-CD/PGK-Puro) and stop sequences between the splicing acceptor (SA) and the beta-gal-neomycin phosphotransferase fusion gene (beta-geo) sequences of the proviral ROSA 26 locus. Correctly targeted, this resulted in ES cells that were Puro resistant but G418 susceptible, and did not express beta-gal. [MGI Ref ID J:64291] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sor MCA, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Mao X; Fujiwara Y; Orkin SH. 1999. Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc Natl Acad Sci U S A 96(9):5037-42. [PubMed: 10220414] [MGI Ref ID J:64291]
Additional References
Egan LJ; Eckmann L; Greten FR; Chae S; Li ZW; Myhre GM; Robine S; Karin M; Kagnoff MF. 2004. IkappaB-kinasebeta-dependent NF-kappaB activation provides radioprotection to the intestinal epithelium. Proc Natl Acad Sci U S A 101(8):2452-7. [PubMed: 14983030] [MGI Ref ID J:88644]
Xin HB; Deng KY; Rishniw M; Ji G; Kotlikoff MI. 2002. Smooth muscle expression of Cre recombinase and eGFP in transgenic mice. Physiol Genomics 10(3):211-5. [PubMed: 12209023] [MGI Ref ID J:81358]
Gt(ROSA)26Sortm1Sho relatedBerger J; Eckert S; Scardigli R; Guillemot F; Gruss P; Stoykova A. 2004. E1-Ngn2/Cre is a new line for regional activation of Cre recombinase in the developing CNS. Genesis 40(4):195-9. [PubMed: 15593326] [MGI Ref ID J:95642]
Blaess S; Graus-Porta D; Belvindrah R; Radakovits R; Pons S; Littlewood-Evans A; Senften M; Guo H; Li Y; Miner JH; Reichardt LF; Muller U. 2004. Beta1-integrins are critical for cerebellar granule cell precursor proliferation. J Neurosci 24(13):3402-12. [PubMed: 15056720] [MGI Ref ID J:96910]
Carlen M; Meletis K; Goritz C; Darsalia V; Evergren E; Tanigaki K; Amendola M; Barnabe-Heider F; Yeung MS; Naldini L; Honjo T; Kokaia Z; Shupliakov O; Cassidy RM; Lindvall O; Frisen J. 2009. Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke. Nat Neurosci 12(3):259-67. [PubMed: 19234458] [MGI Ref ID J:150542]
Casola S; Cattoretti G; Uyttersprot N; Koralov SB; Segal J; Hao Z; Waisman A; Egert A; Ghitza D; Rajewsky K. 2006. Tracking germinal center B cells expressing germ-line immunoglobulin gamma1 transcripts by conditional gene targeting. Proc Natl Acad Sci U S A 103(19):7396-401. [PubMed: 16651521] [MGI Ref ID J:109583]
Chen MJ; Yokomizo T; Zeigler BM; Dzierzak E; Speck NA. 2009. Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter. Nature 457(7231):887-91. [PubMed: 19129762] [MGI Ref ID J:145700]
Egawa T; Eberl G; Taniuchi I; Benlagha K; Geissmann F; Hennighausen L; Bendelac A; Littman DR. 2005. Genetic evidence supporting selection of the valpha14i NKT cell lineage from double-positive thymocyte precursors. Immunity 22(6):705-16. [PubMed: 15963785] [MGI Ref ID J:99111]
Garrick D; Sharpe JA; Arkell R; Dobbie L; Smith AJ; Wood WG; Higgs DR; Gibbons RJ. 2006. Loss of Atrx affects trophoblast development and the pattern of X-inactivation in extraembryonic tissues. PLoS Genet 2(4):e58. [PubMed: 16628246] [MGI Ref ID J:115836]
Graus-Porta D; Blaess S; Senften M; Littlewood-Evans A; Damsky C; Huang Z; Orban P; Klein R; Schittny JC; Muller U. 2001. Beta1-class integrins regulate the development of laminae and folia in the cerebral and cerebellar cortex. Neuron 31(3):367-79. [PubMed: 11516395] [MGI Ref ID J:71122]
Hafner M; Wenk J; Nenci A; Pasparakis M; Scharffetter-Kochanek K; Smyth N; Peters T; Kess D; Holtkotter O; Shephard P; Kudlow JE; Smola H; Haase I; Schippers A; Krieg T; Muller W. 2004. Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein. Genesis 38(4):176-81. [PubMed: 15083518] [MGI Ref ID J:89717]
Haldar M; Hedberg ML; Hockin MF; Capecchi MR. 2009. A CreER-based random induction strategy for modeling translocation-associated sarcomas in mice. Cancer Res 69(8):3657-64. [PubMed: 19351831] [MGI Ref ID J:147728]
Li Z; Chen MJ; Stacy T; Speck NA. 2006. Runx1 function in hematopoiesis is required in cells that express Tek. Blood 107(1):106-10. [PubMed: 16174759] [MGI Ref ID J:125796]
Lobsiger CS; Boillee S; McAlonis-Downes M; Khan AM; Feltri ML; Yamanaka K; Cleveland DW. 2009. Schwann cells expressing dismutase active mutant SOD1 unexpectedly slow disease progression in ALS mice. Proc Natl Acad Sci U S A 106(11):4465-70. [PubMed: 19251638] [MGI Ref ID J:146766]
Lu TL; Chang JL; Liang CC; You LR; Chen CM. 2007. Tumor spectrum, tumor latency and tumor incidence of the pten-deficient mice. PLoS ONE 2(11):e1237. [PubMed: 18043744] [MGI Ref ID J:130367]
Ma Q; Zhou B; Pu WT. 2008. Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity. Dev Biol 323(1):98-104. [PubMed: 18775691] [MGI Ref ID J:141152]
Miclea RL; Karperien M; Bosch CA; van der Horst G; van der Valk MA; Kobayashi T; Kronenberg HM; Rawadi G; Akcakaya P; Lowik CW; Fodde R; Wit JM; Robanus-Maandag EC. 2009. Adenomatous polyposis coli-mediated control of beta-catenin is essential for both chondrogenic and osteogenic differentiation of skeletal precursors. BMC Dev Biol 9:26. [PubMed: 19356224] [MGI Ref ID J:149225]
Morris ZS; McClatchey AI. 2009. Aberrant epithelial morphology and persistent epidermal growth factor receptor signaling in a mouse model of renal carcinoma. Proc Natl Acad Sci U S A 106(24):9767-72. [PubMed: 19487675] [MGI Ref ID J:150071]
Narboux-Neme N; Pavone LM; Avallone L; Zhuang X; Gaspar P. 2008. Serotonin transporter transgenic (SERTcre) mouse line reveals developmental targets of serotonin specific reuptake inhibitors (SSRIs). Neuropharmacology 55(6):994-1005. [PubMed: 18789954] [MGI Ref ID J:142523]
Peng X; Wu X; Druso JE; Wei H; Park AY; Kraus MS; Alcaraz A; Chen J; Chien S; Cerione RA; Guan JL. 2008. Cardiac developmental defects and eccentric right ventricular hypertrophy in cardiomyocyte focal adhesion kinase (FAK) conditional knockout mice. Proc Natl Acad Sci U S A 105(18):6638-43. [PubMed: 18448675] [MGI Ref ID J:134635]
Pratt T; Tian NM; Simpson TI; Mason JO; Price DJ. 2004. The winged helix transcription factor Foxg1 facilitates retinal ganglion cell axon crossing of the ventral midline in the mouse. Development 131(15):3773-84. [PubMed: 15240555] [MGI Ref ID J:92062]
Ramirez A; Page A; Gandarillas A; Zanet J; Pibre S; Vidal M; Tusell L; Genesca A; Whitaker DA; Melton DW; Jorcano JL. 2004. A keratin K5Cre transgenic line appropriate for tissue-specific or generalized Cre-mediated recombination. Genesis 39(1):52-7. [PubMed: 15124227] [MGI Ref ID J:89783]
Rivera-Feliciano J; Lee KH; Kong SW; Rajagopal S; Ma Q; Springer Z; Izumo S; Tabin CJ; Pu WT. 2006. Development of heart valves requires Gata4 expression in endothelial-derived cells. Development 133(18):3607-18. [PubMed: 16914500] [MGI Ref ID J:112458]
Schwander M; Leu M; Stumm M; Dorchies OM; Ruegg UT; Schittny J; Muller U. 2003. Beta1 integrins regulate myoblast fusion and sarcomere assembly. Dev Cell 4(5):673-85. [PubMed: 12737803] [MGI Ref ID J:129360]
Schwander M; Shirasaki R; Pfaff SL; Muller U. 2004. Beta1 integrins in muscle, but not in motor neurons, are required for skeletal muscle innervation. J Neurosci 24(37):8181-91. [PubMed: 15371519] [MGI Ref ID J:146417]
Scott MM; Wylie CJ; Lerch JK; Murphy R; Lobur K; Herlitze S; Jiang W; Conlon RA; Strowbridge BW; Deneris ES. 2005. A genetic approach to access serotonin neurons for in vivo and in vitro studies. Proc Natl Acad Sci U S A 102(45):16472-7. [PubMed: 16251278] [MGI Ref ID J:103436]
Sgaier SK; Millet S; Villanueva MP; Berenshteyn F; Song C; Joyner AL. 2005. Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping. Neuron 45(1):27-40. [PubMed: 15629700] [MGI Ref ID J:95823]
Stenman J; Toresson H; Campbell K. 2003. Identification of two distinct progenitor populations in the lateral ganglionic eminence: implications for striatal and olfactory bulb neurogenesis. J Neurosci 23(1):167-74. [PubMed: 12514213] [MGI Ref ID J:82693]
Stenman JM; Rajagopal J; Carroll TJ; Ishibashi M; McMahon J; McMahon AP. 2008. Canonical Wnt signaling regulates organ-specific assembly and differentiation of CNS vasculature. Science 322(5905):1247-50. [PubMed: 19023080] [MGI Ref ID J:142352]
Vitelli F; Huynh T; Baldini A. 2009. Gain of function of Tbx1 affects pharyngeal and heart development in the mouse. Genesis 47(3):188-95. [PubMed: 19253341] [MGI Ref ID J:146886]
Wrobel CN; Mutch CA; Swaminathan S; Taketo MM; Chenn A. 2007. Persistent expression of stabilized beta-catenin delays maturation of radial glial cells into intermediate progenitors. Dev Biol 309(2):285-97. [PubMed: 17706960] [MGI Ref ID J:124867]
Zadelaar SM; Boesten LS; Pires NM; van Nieuwkoop A; Biessen EA; Jukema W; Havekes LM; van Vlijmen BJ; Willems van Dijk K. 2006. Local cre-mediated gene recombination in vascular smooth muscle cells in mice. Transgenic Res 15(1):31-6. [PubMed: 16475008] [MGI Ref ID J:106456]
Zeisberg EM; Ma Q; Juraszek AL; Moses K; Schwartz RJ; Izumo S; Pu WT. 2005. Morphogenesis of the right ventricle requires myocardial expression of Gata4. J Clin Invest 115(6):1522-1531. [PubMed: 15902305] [MGI Ref ID J:99203]
Zeisberg EM; Potenta S; Xie L; Zeisberg M; Kalluri R. 2007. Discovery of endothelial to mesenchymal transition as a source for carcinoma-associated fibroblasts. Cancer Res 67(21):10123-8. [PubMed: 17974953] [MGI Ref ID J:127148]
Zhang DJ; Wang Q; Wei J; Baimukanova G; Buchholz F; Stewart AF; Mao X; Killeen N. 2005. Selective expression of the Cre recombinase in late-stage thymocytes using the distal promoter of the Lck gene. J Immunol 174(11):6725-31. [PubMed: 15905512] [MGI Ref ID J:99042]
Zhou B; Ma Q; Kong SW; Hu Y; Campbell PH; McGowan FX; Ackerman KG; Wu B; Zhou B; Tevosian SG; Pu WT. 2009. Fog2 is critical for cardiac function and maintenance of coronary vasculature in the adult mouse heart. J Clin Invest 119(6):1462-76. [PubMed: 19411759] [MGI Ref ID J:150452]
Zhou B; Ma Q; Rajagopal S; Wu SM; Domian I; Rivera-Feliciano J; Jiang D; von Gise A; Ikeda S; Chien KR; Pu WT. 2008. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454(7200):109-13. [PubMed: 18568026] [MGI Ref ID J:137426]
Zhuang X; Masson J; Gingrich JA; Rayport S; Hen R. 2005. Targeted gene expression in dopamine and serotonin neurons of the mouse brain. J Neurosci Methods 143(1):27-32. [PubMed: 15763133] [MGI Ref ID J:115426]
de Alboran IM; Baena E; Martinez-A C. 2004. c-Myc-deficient B lymphocytes are resistant to spontaneous and induced cell death. Cell Death Differ 11(1):61-8. [PubMed: 12970677] [MGI Ref ID J:115648]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently On Hold.
To register your interest in this strain go to the Strain Interest Form.
To request more information use the Customer Support Contact Form or call 1.800.422.6423 or 207.288.5845
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Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $157.20 Female or Male Homozygous for Gt(ROSA)26Sortm1Sho
Pairs /Price (US dollars $) Pair Genotype $314.40 Homozygous for Gt(ROSA)26Sortm1Sho x Homozygous for Gt(ROSA)26Sortm1Sho
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $204.40 Female or Male Homozygous for Gt(ROSA)26Sortm1Sho
Pairs /Price (US dollars $) Pair Genotype $408.80 Homozygous for Gt(ROSA)26Sortm1Sho x Homozygous for Gt(ROSA)26Sortm1Sho
Additional Supply Details
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Supply Details
| Standard Supply | This strain is currently on HOLD - Contact Customer Service for more information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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