Description

Strain Information

Type Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation F191+D2N2 3

Appearance
dilute brown, elongated axial skeleton and digits
Related Genotype: a/a Tyrp1^b/Tyrp1^b Myo5a^d/Myo5a^d Npr3^lgj-2J/Npr3^lgj-2J

Description
Homozygous mutants are identifiable as young as six days of age by their elongated bodies and digits and a conical extension of the body at the base of the tail. Older mice are extremely thin and exhibit arachnodactyly, thoracic kyphosis and, often, kinks in the tail and/or sacrum. Upon necropsy, mice are found to lack normal body fat deposits. No significant craniofacial defects have been observed. Skeletal analysis demonstrated delayed endochondrial ossification, most obvious in the hind- and forepaws but affecting the entire skeleton. Retarded ossification was apparent in neonates and presumably occurs in utero.Both male and female Npr3^lgj-2J/Npr3^lgj-2Jmice are fertile and can breed.

An earlier mutation at this locus, Npr3^lgj, in the BALB/cJ inbred strain (stock number 003206) produces a similar phenotype. However, homozygous males of this stock number 003206 cannot reproduce because their more extreme spinal defect prevents them from mounting.

Development
The Npr3^lgj-2J mutation occurred spontaneously in 1996 in the DBA/2J inbred strain at The Jackson Laboratory. It was initially outcrossed once to a B6D2F1 female via ovarian transplant and subsequently has been maintained through backcross-intercross breeding. Thus, this strain does have a small amount of C57BL/6J in its genetic background but the mutant allele arose on a DBA/2J chromosomal segment. This mutation was originally named lgj^2J, longjohn-2J. When it was identified as a mutation of the Npr3 gene, it was renamed Npr3^lgj-2J.

Control Information

	Control
	000671 DBA/2J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Npr3

003506	B6;C-Npr3lgj/J
008188	C57BL/6J-Npr3lgj-3J/J
008254	NOD/ShiLtJ-Npr3lgj-4J/J
002812	STOCK Npr3stri/PasEiJ

View Strains carrying other alleles of Npr3     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Npr3^lgj-2J/Npr3^lgj-2J

        DBA/2J

• life span-post-weaning/aging
• *normal* life span-post-weaning/aging (MGI Ref ID J:57460)
• premature death (MGI Ref ID J:57460)
  • sudden death is common in adulthood
• skeleton phenotype
• abnormal axial skeleton morphology (MGI Ref ID J:57460)
  • easily distinguished from wild-type sibs as early as 6 days of age
• abnormal thoracic cage (MGI Ref ID J:57460)
• abnormal vertebrae morphology (MGI Ref ID J:57460)
• abnormal caudal vertebrae morphology (MGI Ref ID J:57460)
• abnormal sacral vertebrae morphology (MGI Ref ID J:57460)
  • sacral kinks are observed even in young mice
• kyphosis (MGI Ref ID J:57460)
  • thoracic involvement
• abnormal skeleton development (MGI Ref ID J:57460)
• abnormal skeleton extremities morphology (MGI Ref ID J:57460)
• limbs/digits/tail phenotype
• abnormal skeleton extremities morphology (MGI Ref ID J:57460)
• abnormal caudal vertebrae morphology (MGI Ref ID J:57460)
• arachnodactyly (MGI Ref ID J:57460)
• kinked tail (MGI Ref ID J:57460)
• adipose tissue phenotype
• abnormal adipose tissue morphology (MGI Ref ID J:57460)
• abnormal abdominal fat pad morphology (MGI Ref ID J:57460)
• abnormal adipose tissue distribution (MGI Ref ID J:57460)
• craniofacial phenotype
• *normal* craniofacial phenotype (MGI Ref ID J:57460)
  • no gross abnormalities in craniofacial structures

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Npr3^lgj-2J related

Cardiovascular Research
Hypertension

Developmental Biology Research
Skeletal Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Npr3lgj-2J
Allele Name		longjohn 2 Jackson
Allele Type		Spontaneous
Common Name(s)		lgj-2J;
Strain of Origin		DBA/2J
Gene Symbol and Name		Npr3, natriuretic peptide receptor 3
Chromosome		15
Gene Common Name(s)		ANP-CR; ANPRC; B430320C24Rik; C81222; GUCY2B; NPR-C; NPRC; Nppc receptor; RIKEN cDNA B430320C24 gene; expressed sequence C81222; lgj; longjohn; stri; strigosus;
General Note		Genbank: AF131863
Molecular Note		A C-to-T transition mutation at position 283 is introduces a premature stop codon in the encoded protein. [MGI Ref ID J:57460]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Npr3^lgj-2J related

Jaubert J; Jaubert F; Martin N; Washburn LL; Lee BK; Eicher EM; Guenet JL. 1999. Three new allelic mouse mutations that cause skeletal overgrowth involve the natriuretic peptide receptor C gene (Npr3). Proc Natl Acad Sci U S A 96(18):10278-83. [PubMed: 10468599]  [MGI Ref ID J:57460]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Mouse Mutant Resource collection.

Control Information

	Control
	000671 DBA/2J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.