Strain Name:

B6.129-Blmhtm1Geh/J

Stock Number:

003509

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129X1 x 129S1 via R1 (+Kitl-SlJ) ES cell line
 
Donating InvestigatorDr. John S. Lazo,   University of Pittsburgh

Description
Bleomycin (BLM) is a clinically used glycopeptide anticancer agent. It is deaminated in vitro by BLMH. Blmh null mice have decreased viability and fertitility. Only about 65% of the expected number survive the neonatal period. Mice lacking Blmh exhibit variably penetrant tail dermatitis that resembles rodent ringtail. This resembles skin lesions in humans with pellagra, necrolytic migratory erythema, and acrodermatitis enteropathica. Null mice also are more sensitive to acute BLM lethality and develop pulmonary fibrosis following BLM treatment.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Alzheimer Disease; AD   (BLMH)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Blmhtm1Geh/Blmhtm1Geh

        either: (involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)
  • mortality/aging
  • increased sensitivity to xenobiotic induced morbidity/mortality
    • bleomycin doses that were without effect in wild-type mice elicited lethality (50 or 100 mg/kg) in homozygous null mice   (MGI Ref ID J:54585)
  • partial neonatal lethality
    • approximately 36% of homozygotes died as neonates; those surviving the neonatal period were fertile and appeared healthy   (MGI Ref ID J:54585)
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • the skin lesions were not caused by aggressive behavior: both male and female mutants were housed individually without resolution of the tail dermatitis   (MGI Ref ID J:54585)
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • homozygotes did not display angitis or vascular thrombosis   (MGI Ref ID J:54585)
  • growth/size/body phenotype
  • decreased body size
    • homozygotes frequently appeared smaller than wild-type or heterozygous littermates   (MGI Ref ID J:54585)
  • homeostasis/metabolism phenotype
  • increased physiological sensitivity to xenobiotic
    • homozygotes were hypersensitive to the deleterious effects of bleomycin   (MGI Ref ID J:54585)
    • bleomycin doses that were without effect in wild-type mice elicited either pulmonary fibrosis (25 mg/kg) in homozygous null mice   (MGI Ref ID J:54585)
  • increased sensitivity to xenobiotic induced morbidity/mortality
    • bleomycin doses that were without effect in wild-type mice elicited lethality (50 or 100 mg/kg) in homozygous null mice   (MGI Ref ID J:54585)
  • limbs/digits/tail phenotype
  • abnormal tail morphology
    • the gross tail lesions resembled rodent ringtail, and were frequently resolved by weaning   (MGI Ref ID J:54585)
  • immune system phenotype
  • dermatitis
    • dermatitis on the tail only   (MGI Ref ID J:54585)
    • mutants without dermatitis on the tail develop tail dermatitis when kept in a low-humidity atmosphere   (MGI Ref ID J:54585)
  • integument phenotype
  • dermatitis
    • dermatitis on the tail only   (MGI Ref ID J:54585)
    • mutants without dermatitis on the tail develop tail dermatitis when kept in a low-humidity atmosphere   (MGI Ref ID J:54585)
  • scaly skin
    • most newborns appeared normal except for a dermatopathy presenting initially as a mild ichthyosis with generalized scaling and sloughing of the skin over the entire body   (MGI Ref ID J:54585)
    • at ~7-10 days, the scaling became resolved except on the tail   (MGI Ref ID J:54585)
    • no abnormalities were observed in other tissues   (MGI Ref ID J:54585)
  • thick epidermis
    • similar to ringtail, the tail dermatitis included thickening of the epidermis (parakeratotic hyperkeratosis and acanthosis), areas of necrosis, and neutrophil infiltrate   (MGI Ref ID J:54585)
    • unlike ringtail, ballooning degeneration of the upper stratum spinosum and palor were observed in some mutant tails   (MGI Ref ID J:54585)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Blmhtm1Geh related

Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence
      Lymphomas
      Other Tissues/Organs
Toxicology

Dermatology Research
Skin and Hair Texture Defects

Developmental Biology Research
Skin and Hair Texture Defects

Endocrine Deficiency Research
Skin Defects

Immunology, Inflammation and Autoimmunity Research
Inflammation

Internal/Organ Research
Lung Defects

Neurobiology Research
Neurodegeneration

Research Tools
Cancer Research
Dermatology Research
Metabolism Research
Toxicology Research
      drug metabolism

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Blmhtm1Geh
Allele Name targeted mutation 1, Gregg E Homanics
Allele Type Targeted (Null/Knockout)
Common Name(s) Bh-; Blmhtm2Geh;
Mutation Made By Donald Schwartz,   Wayne State Univ School of Medicine
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Blmh, bleomycin hydrolase
Chromosome 11
Gene Common Name(s) AI035728; BH; BMH; MGC:36899; MGC:37104; MGC:39026; expressed sequence AI035728;
Molecular Note A genomic fragment containing exon 3 and flanking DNA was replaced with a neomycin selection cassette. The deleted sequences include the region encoding the catalytic cysteine of the protein. Northern blot analysis on total embryonic RNA derived from homozygous mice demonstrated that no transcript was produced from this allele. Western blot and enzymatic activity assays confirmed that no functional protein was made in homozygous mice. [MGI Ref ID J:54585]

Genotyping

Genotyping Information

Genotyping Protocols

Blmhtm1Geh, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Blmhtm1Geh related

Montoya SE; Thiels E; Card JP; Lazo JS. 2007. Astrogliosis and behavioral changes in mice lacking the neutral cysteine protease bleomycin hydrolase. Neuroscience 146(3):890-900. [PubMed: 17391860]  [MGI Ref ID J:122060]

Schwartz DR; Homanics GE; Hoyt DG; Klein E; Abernethy J; Lazo JS. 1999. The neutral cysteine protease bleomycin hydrolase is essential for epidermal integrity and bleomycin resistance. Proc Natl Acad Sci U S A 96(8):4680-5. [PubMed: 10200322]  [MGI Ref ID J:54585]

Towne CF; York IA; Watkin LB; Lazo JS; Rock KL. 2007. Analysis of the role of bleomycin hydrolase in antigen presentation and the generation of CD8 T cell responses. J Immunol 178(11):6923-30. [PubMed: 17513741]  [MGI Ref ID J:147841]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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