Strain Name:

BALB/c-Il4ratm1Sz/J

Stock Number:

003514

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Homozygous Il4ra knockout mice show no overt phenotypic abnormalities, but exhibit a loss of IL4-mediated signal transduction.

Description

Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
GenerationF?+F26 (19-MAY-11)
Generation Definitions
 
Donating InvestigatorDr. Leonard D. Shultz,   The Jackson Laboratory

Appearance
albino
Related Genotype: A/A Tyrp1b/Tyrp1b Tyrc/Tyrc

Description
Homozygous Il4ra (interleukin 4 receptor, alpha) knockout mice show no overt phenotypic abnormalities, but exhibit a loss of IL4 signal transduction. This results in a diminished TH2 helper T cell response to helminthic parasite infections. These mutant mice provide a tool for analyzing the role of IL4/IL4RA in immunological pathways, including those that prime TH2 responses in infectious disease and other pathologies.

Development
Exons 7, 8, and 9 were replaced with a PGKneomycin resistance cassette inserted in reverse transcriptional orientation. The mutation was created through homologous recombination in BALB/cJ-derived BALB/c-I embryonic stem (ES) cells. Resultant chimeric males were bred to BALB/cJ females once before sibling matings were set up by the donating laboratory.

Control Information

  Control
   000651 BALB/cJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Il4ra
007680   C.129X1-Il4ratm1Tch/J
007746   C.129X1-Il4ratm2Tch/J
012709   C.129X1-Il4ratm3.1Tch/J
View Strains carrying other alleles of Il4ra     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Human Immunodeficiency Virus Type 1, Susceptibility to   (IL4R)
IgE Responsiveness, Atopic; IGER   (IL4R)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Il4ratm1Sz/Il4ratm1Sz

        BALB/c-Il4ratm1Sz/J
  • immune system phenotype
  • abnormal T-helper 2 physiology
    • mice are unable to mount an effective Th2 response to N. brasiliensis bacteria as determined by Th2 cyotkine production by T cells   (MGI Ref ID J:43656)
  • abnormal microglial cell morphology
    • microglial cells fail to express the cell marker Ym1 (Chi3l3)   (MGI Ref ID J:125605)
  • altered susceptibility to infection
    • mice immunized with recombinant vvG (vaccinia virus expressing the G protein of respiratory syncytial virus (RSV)) then challenged with RSV display lower percentages and total numbers of eosinophils in the bronchoalveolar lavage (BAL) compared to wild-type mice   (MGI Ref ID J:131994)
  • decreased IgE level
    • lower circulating levels of IgE in normal and bacterial infected mice   (MGI Ref ID J:43656)
  • decreased IgG1 level
    • lower circulating levels in normal and bacterial infected mice   (MGI Ref ID J:43656)
  • decreased interleukin-10 secretion
    • decrease production by T cells from mice infected with N. brasiliensis bacteria   (MGI Ref ID J:43656)
  • decreased interleukin-4 secretion
    • decrease production of IL-4 by T cells from mice infected with N. brasiliensis bacteria   (MGI Ref ID J:43656)
  • decreased interleukin-5 secretion
    • decrease production of IL-5 by T cells from mice infected with N. brasiliensis bacteria   (MGI Ref ID J:43656)
  • increased IgG2a level   (MGI Ref ID J:43656)
  • hematopoietic system phenotype
  • abnormal T-helper 2 physiology
    • mice are unable to mount an effective Th2 response to N. brasiliensis bacteria as determined by Th2 cyotkine production by T cells   (MGI Ref ID J:43656)
  • abnormal microglial cell morphology
    • microglial cells fail to express the cell marker Ym1 (Chi3l3)   (MGI Ref ID J:125605)
  • decreased IgE level
    • lower circulating levels of IgE in normal and bacterial infected mice   (MGI Ref ID J:43656)
  • decreased IgG1 level
    • lower circulating levels in normal and bacterial infected mice   (MGI Ref ID J:43656)
  • increased IgG2a level   (MGI Ref ID J:43656)
  • nervous system phenotype
  • abnormal microglial cell morphology
    • microglial cells fail to express the cell marker Ym1 (Chi3l3)   (MGI Ref ID J:125605)

Il4ratm1Sz/Il4ratm1Sz

        BALB/c-Il4ratm1Sz
  • immune system phenotype
  • increased IgG2a level
    • mice make increased levels of IgG2 in response to N. brasiliensis infection   (MGI Ref ID J:110429)
  • increased susceptibility to parasitic infection
    • 15 days after inoculation with N. brasiliensis, intestines contain large numbers of adult worms while controls have expelled the worms by this time point   (MGI Ref ID J:110429)
  • hematopoietic system phenotype
  • increased IgG2a level
    • mice make increased levels of IgG2 in response to N. brasiliensis infection   (MGI Ref ID J:110429)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Il4ratm1Sz related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Il4ratm1Sz
Allele Name targeted mutation 1, Leonard Shultz
Allele Type Targeted (Null/Knockout)
Common Name(s) IL-4R KO; IL-4R-; IL-4Ralpha-; Il-4ralphatm1Sz; Il4ratm1;
Mutation Made ByDr. Leonard Shultz,   The Jackson Laboratory
Strain of OriginBALB/cJ
ES Cell Line NameBALB/c-I
ES Cell Line StrainBALB/cJ
Gene Symbol and Name Il4ra, interleukin 4 receptor, alpha
Chromosome 7
Gene Common Name(s) CD124; IL-4 receptor alpha chain; IL-4RA; Il4r; interleukin 4 receptor;
Molecular Note A neomycin resistance cassette replaced exons 7 - 9 of the gene. [MGI Ref ID J:43656]

Genotyping

Genotyping Information

Genotyping Protocols

Il4ratm1Sz, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Il4ratm1Sz related

Akue AD; Lee JY; Jameson SC. 2012. Derivation and maintenance of virtual memory CD8 T cells. J Immunol 188(6):2516-23. [PubMed: 22308307]  [MGI Ref ID J:181867]

Atochina O; Da'dara AA; Walker M; Harn DA. 2008. The immunomodulatory glycan LNFPIII initiates alternative activation of murine macrophages in vivo. Immunology 125(1):111-21. [PubMed: 18373667]  [MGI Ref ID J:142776]

Auderset F; Schuster S; Fasnacht N; Coutaz M; Charmoy M; Koch U; Favre S; Wilson A; Trottein F; Alexander J; Luther SA; MacDonald HR; Radtke F; Tacchini-Cottier F. 2013. Notch signaling regulates follicular helper T cell differentiation. J Immunol 191(5):2344-50. [PubMed: 23918982]  [MGI Ref ID J:205797]

Bian K; Zhong M; Harari Y; Lai M; Weisbrodt N; Murad F. 2005. Helminth regulation of host IL-4Ralpha/Stat6 signaling: mechanism underlying NOS-2 inhibition by Trichinella spiralis. Proc Natl Acad Sci U S A 102(11):3936-41. [PubMed: 15741272]  [MGI Ref ID J:97167]

Castilow EM; Meyerholz DK; Varga SM. 2008. IL-13 is required for eosinophil entry into the lung during respiratory syncytial virus vaccine-enhanced disease. J Immunol 180(4):2376-84. [PubMed: 18250447]  [MGI Ref ID J:131994]

Chen F; Liu Z; Wu W; Rozo C; Bowdridge S; Millman A; Van Rooijen N; Urban JF Jr; Wynn TA; Gause WC. 2012. An essential role for T(H)2-type responses in limiting acute tissue damage during experimental helminth infection. Nat Med 18(2):260-6. [PubMed: 22245779]  [MGI Ref ID J:181182]

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DeNardo DG; Barreto JB; Andreu P; Vasquez L; Tawfik D; Kolhatkar N; Coussens LM. 2009. CD4(+) T cells regulate pulmonary metastasis of mammary carcinomas by enhancing protumor properties of macrophages. Cancer Cell 16(2):91-102. [PubMed: 19647220]  [MGI Ref ID J:151976]

Dickgreber N; Farrand KJ; van Panhuys N; Knight DA; McKee SJ; Chong ML; Miranda-Hernandez S; Baxter AG; Locksley RM; Le Gros G; Hermans IF. 2012. Immature murine NKT cells pass through a stage of developmentally programmed innate IL-4 secretion. J Leukoc Biol 92(5):999-1009. [PubMed: 22941735]  [MGI Ref ID J:190015]

Egawa M; Mukai K; Yoshikawa S; Iki M; Mukaida N; Kawano Y; Minegishi Y; Karasuyama H. 2013. Inflammatory Monocytes Recruited to Allergic Skin Acquire an Anti-inflammatory M2 Phenotype via Basophil-Derived Interleukin-4. Immunity 38(3):570-80. [PubMed: 23434060]  [MGI Ref ID J:194750]

Elgueta R; Sepulveda FE; Vilches F; Vargas L; Mora JR; Bono MR; Rosemblatt M. 2008. Imprinting of CCR9 on CD4 T cells requires IL-4 signaling on mesenteric lymph node dendritic cells. J Immunol 180(10):6501-7. [PubMed: 18453568]  [MGI Ref ID J:134963]

Finkelman FD; Morris SC; Orekhova T; Mori M; Donaldson D; Reiner SL; Reilly NL; Schopf L; Urban JF Jr. 2000. Stat6 regulation of in vivo IL-4 responses. J Immunol 164(5):2303-10. [PubMed: 10679064]  [MGI Ref ID J:112038]

Fish SC; Donaldson DD; Goldman SJ; Williams CM; Kasaian MT. 2005. IgE generation and mast cell effector function in mice deficient in IL-4 and IL-13. J Immunol 174(12):7716-24. [PubMed: 15944273]  [MGI Ref ID J:100883]

Forbes EE; Groschwitz K; Abonia JP; Brandt EB; Cohen E; Blanchard C; Ahrens R; Seidu L; McKenzie A; Strait R; Finkelman FD; Foster PS; Matthaei KI; Rothenberg ME; Hogan SP. 2008. IL-9- and mast cell-mediated intestinal permeability predisposes to oral antigen hypersensitivity. J Exp Med 205(4):897-913. [PubMed: 18378796]  [MGI Ref ID J:133972]

Gallina G; Dolcetti L; Serafini P; De Santo C; Marigo I; Colombo MP; Basso G; Brombacher F; Borrello I; Zanovello P; Bicciato S; Bronte V. 2006. Tumors induce a subset of inflammatory monocytes with immunosuppressive activity on CD8+ T cells. J Clin Invest 116(10):2777-90. [PubMed: 17016559]  [MGI Ref ID J:114986]

Gallo E; Katzman S; Villarino AV. 2012. IL-13-producing Th1 and Th17 cells characterize adaptive responses to both self and foreign antigens. Eur J Immunol 42(9):2322-8. [PubMed: 22684943]  [MGI Ref ID J:187947]

Gaupp S; Cannella B; Raine CS. 2008. Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines. Am J Pathol 173(1):119-29. [PubMed: 18535177]  [MGI Ref ID J:137384]

Greenblatt MB; Sargent JL; Farina G; Tsang K; Lafyatis R; Glimcher LH; Whitfield ML; Aliprantis AO. 2012. Interspecies comparison of human and murine scleroderma reveals IL-13 and CCL2 as disease subset-specific targets. Am J Pathol 180(3):1080-94. [PubMed: 22245215]  [MGI Ref ID J:181939]

Han H; Headley MB; Xu W; Comeau MR; Zhou B; Ziegler SF. 2013. Thymic stromal lymphopoietin amplifies the differentiation of alternatively activated macrophages. J Immunol 190(3):904-12. [PubMed: 23275605]  [MGI Ref ID J:193032]

Harris DP; Goodrich S; Mohrs K; Mohrs M; Lund FE. 2005. Cutting edge: the development of IL-4-producing B cells (B effector 2 cells) is controlled by IL-4, IL-4 receptor alpha, and Th2 cells. J Immunol 175(11):7103-7. [PubMed: 16301612]  [MGI Ref ID J:122200]

Hayashi N; Yoshimoto T; Izuhara K; Matsui K; Tanaka T; Nakanishi K. 2007. T helper 1 cells stimulated with ovalbumin and IL-18 induce airway hyperresponsiveness and lung fibrosis by IFN-{gamma} and IL-13 production. Proc Natl Acad Sci U S A 104(37):14765-70. [PubMed: 17766435]  [MGI Ref ID J:124973]

Herbert DR; Orekov T; Perkins C; Rothenberg ME; Finkelman FD. 2008. IL-4R{alpha} Expression by Bone Marrow-Derived Cells Is Necessary and Sufficient for Host Protection against Acute Schistosomiasis. J Immunol 180(7):4948-55. [PubMed: 18354220]  [MGI Ref ID J:133371]

Herbert DR; Orekov T; Roloson A; Ilies M; Perkins C; O'Brien W; Cederbaum S; Christianson DW; Zimmermann N; Rothenberg ME; Finkelman FD. 2010. Arginase I suppresses IL-12/IL-23p40-driven intestinal inflammation during acute schistosomiasis. J Immunol 184(11):6438-46. [PubMed: 20483789]  [MGI Ref ID J:161215]

Hill NJ; Stotland AB; Sarvetnick NE. 2007. Distinct regulation of autoreactive CD4 T cell expansion by interleukin-4 under conditions of lymphopenia. J Leukoc Biol 81(3):757-65. [PubMed: 17164429]  [MGI Ref ID J:118599]

Horsley V; Jansen KM; Mills ST; Pavlath GK. 2003. IL-4 acts as a myoblast recruitment factor during mammalian muscle growth. Cell 113(4):483-94. [PubMed: 12757709]  [MGI Ref ID J:107688]

Hsieh YJ; Fu CL; Hsieh MH. 2014. Helminth-induced interleukin-4 abrogates invariant natural killer T cell activation-associated clearance of bacterial infection. Infect Immun 82(5):2087-97. [PubMed: 24643536]  [MGI Ref ID J:209998]

Huang B; Pan PY; Li Q; Sato AI; Levy DE; Bromberg J; Divino CM; Chen SH. 2006. Gr-1+CD115+ immature myeloid suppressor cells mediate the development of tumor-induced T regulatory cells and T-cell anergy in tumor-bearing host. Cancer Res 66(2):1123-31. [PubMed: 16424049]  [MGI Ref ID J:106544]

Huang W; Huang F; Kannan AK; Hu J; August A. 2014. ITK tunes IL-4-induced development of innate memory CD8+ T cells in a gammadelta T and invariant NKT cell-independent manner. J Leukoc Biol 96(1):55-63. [PubMed: 24620029]  [MGI Ref ID J:212002]

Jaffar Z; Ferrini ME; Buford MC; Fitzgerald GA; Roberts K. 2007. Prostaglandin I2-IP signaling blocks allergic pulmonary inflammation by preventing recruitment of CD4+ Th2 cells into the airways in a mouse model of asthma. J Immunol 179(9):6193-203. [PubMed: 17947695]  [MGI Ref ID J:152991]

Jankovic D; Kullberg MC; Noben-Trauth N; Caspar P; Paul WE; Sher A. 2000. Single cell analysis reveals that IL-4 receptor/Stat6 signaling is not required for the in vivo or in vitro development of CD4+ lymphocytes with a Th2 cytokine profile. J Immunol 164(6):3047-55. [PubMed: 10706693]  [MGI Ref ID J:60908]

Jankovic D; Kullberg MC; Noben-Trauth N; Caspar P; Ward JM; Cheever AW; Paul WE; Sher A. 1999. Schistosome-infected IL-4 receptor knockout (KO) mice, in contrast to IL-4 KO mice, fail to develop granulomatous pathology while maintaining the same lymphokine expression profile. J Immunol 163(1):337-42. [PubMed: 10384133]  [MGI Ref ID J:55638]

Jessup HK; Brewer AW; Omori M; Rickel EA; Budelsky AL; Yoon BR; Ziegler SF; Comeau MR. 2008. Intradermal administration of thymic stromal lymphopoietin induces a T cell- and eosinophil-dependent systemic Th2 inflammatory response. J Immunol 181(6):4311-9. [PubMed: 18768889]  [MGI Ref ID J:139079]

Jones TG; Hallgren J; Humbles A; Burwell T; Finkelman FD; Alcaide P; Austen KF; Gurish MF. 2009. Antigen-induced increases in pulmonary mast cell progenitor numbers depend on IL-9 and CD1d-restricted NKT cells. J Immunol 183(8):5251-60. [PubMed: 19783672]  [MGI Ref ID J:153832]

Kelly-Welch AE; Melo ME; Smith E; Ford AQ; Haudenschild C; Noben-Trauth N; Keegan AD. 2004. Complex role of the IL-4 receptor alpha in a murine model of airway inflammation: expression of the IL-4 receptor alpha on nonlymphoid cells of bone marrow origin contributes to severity of inflammation. J Immunol 172(7):4545-55. [PubMed: 15034072]  [MGI Ref ID J:88718]

Ketavarapu JM; Rodriguez AR; Yu JJ; Cong Y; Murthy AK; Forsthuber TG; Guentzel MN; Klose KE; Berton MT; Arulanandam BP. 2008. Mast cells inhibit intramacrophage Francisella tularensis replication via contact and secreted products including IL-4. Proc Natl Acad Sci U S A 105(27):9313-8. [PubMed: 18591675]  [MGI Ref ID J:138191]

Khodoun M; Lewis CC; Yang JQ; Orekov T; Potter C; Wynn T; Mentink-Kane M; Hershey GK; Wills-Karp M; Finkelman FD. 2007. Differences in expression, affinity, and function of soluble (s)IL-4Ralpha and sIL-13Ralpha2 suggest opposite effects on allergic responses. J Immunol 179(10):6429-38. [PubMed: 17982031]  [MGI Ref ID J:153871]

Kim DH; Chang WS; Lee YS; Lee KA; Kim YK; Kwon BS; Kang CY. 2008. 4-1BB engagement costimulates NKT cell activation and exacerbates NKT cell ligand-induced airway hyperresponsiveness and inflammation. J Immunol 180(4):2062-8. [PubMed: 18250411]  [MGI Ref ID J:131924]

Knott ML; Matthaei KI; Foster PS; Dent LA. 2009. The roles of eotaxin and the STAT6 signalling pathway in eosinophil recruitment and host resistance to the nematodes Nippostrongylus brasiliensis and Heligmosomoides bakeri. Mol Immunol 46(13):2714-22. [PubMed: 19535141]  [MGI Ref ID J:151720]

Kohanbash G; McKaveney K; Sakaki M; Ueda R; Mintz AH; Amankulor N; Fujita M; Ohlfest JR; Okada H. 2013. GM-CSF promotes the immunosuppressive activity of glioma-infiltrating myeloid cells through interleukin-4 receptor-alpha. Cancer Res 73(21):6413-23. [PubMed: 24030977]  [MGI Ref ID J:205065]

Koller FL; Hwang DG; Dozier EA; Fingleton B. 2010. Epithelial interleukin-4 receptor expression promotes colon tumor growth. Carcinogenesis 31(6):1010-7. [PubMed: 20176658]  [MGI Ref ID J:160716]

Kosiewicz MM; Alard P; Liang S; Clark SL. 2004. Mechanisms of tolerance induced by transforming growth factor-beta-treated antigen-presenting cells: CD8 regulatory T cells inhibit the effector phase of the immune response in primed mice through a mechanism involving Fas ligand. Int Immunol 16(5):697-706. [PubMed: 15096489]  [MGI Ref ID J:89454]

Lee CG; Homer RJ; Cohn L; Link H; Jung S; Craft JE; Graham BS; Johnson TR; Elias JA. 2002. Transgenic overexpression of interleukin (IL)-10 in the lung causes mucus metaplasia, tissue inflammation, and airway remodeling via IL-13-dependent and -independent pathways. J Biol Chem 277(38):35466-74. [PubMed: 12107190]  [MGI Ref ID J:79116]

Lee YJ; Holzapfel KL; Zhu J; Jameson SC; Hogquist KA. 2013. Steady-state production of IL-4 modulates immunity in mouse strains and is determined by lineage diversity of iNKT cells. Nat Immunol 14(11):1146-54. [PubMed: 24097110]  [MGI Ref ID J:208662]

Liu Z; Liu Q; Hamed H; Anthony RM; Foster A; Finkelman FD; Urban JF Jr; Gause WC. 2005. IL-2 and autocrine IL-4 drive the in vivo development of antigen-specific Th2 T cells elicited by nematode parasites. J Immunol 174(4):2242-9. [PubMed: 15699158]  [MGI Ref ID J:96546]

Livraghi A; Grubb BR; Hudson EJ; Wilkinson KJ; Sheehan JK; Mall MA; O'Neal WK; Boucher RC; Randell SH. 2009. Airway and lung pathology due to mucosal surface dehydration in {beta}-epithelial Na+ channel-overexpressing mice: role of TNF-{alpha} and IL-4R{alpha} signaling, influence of neonatal development, and limited efficacy of glucocorticoid treatment. J Immunol 182(7):4357-67. [PubMed: 19299736]  [MGI Ref ID J:147194]

Maldonado RA; Soriano MA; Perdomo LC; Sigrist K; Irvine DJ; Decker T; Glimcher LH. 2009. Control of T helper cell differentiation through cytokine receptor inclusion in the immunological synapse. J Exp Med 206(4):877-92. [PubMed: 19349465]  [MGI Ref ID J:147862]

Masic A; Hurdayal R; Nieuwenhuizen NE; Brombacher F; Moll H. 2012. Dendritic cell-mediated vaccination relies on interleukin-4 receptor signaling to avoid tissue damage after Leishmania major infection of BALB/c mice. PLoS Negl Trop Dis 6(7):e1721. [PubMed: 22802978]  [MGI Ref ID J:192790]

Matsuda JL; Gapin L; Baron JL; Sidobre S; Stetson DB; Mohrs M; Locksley RM; Kronenberg M. 2003. Mouse V alpha 14i natural killer T cells are resistant to cytokine polarization in vivo. Proc Natl Acad Sci U S A 100(14):8395-400. [PubMed: 12829795]  [MGI Ref ID J:126209]

Mattes J; Yang M; Siqueira A; Clark K; MacKenzie J; McKenzie AN; Webb DC; Matthaei KI; Foster PS. 2001. IL-13 induces airways hyperreactivity independently of the IL-4R alpha chain in the allergic lung. J Immunol 167(3):1683-92. [PubMed: 11466392]  [MGI Ref ID J:120464]

McCaughtry TM; Etzensperger R; Alag A; Tai X; Kurtulus S; Park JH; Grinberg A; Love P; Feigenbaum L; Erman B; Singer A. 2012. Conditional deletion of cytokine receptor chains reveals that IL-7 and IL-15 specify CD8 cytotoxic lineage fate in the thymus. J Exp Med 209(12):2263-76. [PubMed: 23109710]  [MGI Ref ID J:190889]

McGaha T; Saito S; Phelps RG; Gordon R; Noben-Trauth N; Paul WE; Bona C. 2001. Lack of skin fibrosis in tight skin (TSK) mice with targeted mutation in the interleukin-4R alpha and transforming growth factor-beta genes. J Invest Dermatol 116(1):136-43. [PubMed: 11168809]  [MGI Ref ID J:68448]

McHedlidze T; Waldner M; Zopf S; Walker J; Rankin AL; Schuchmann M; Voehringer D; McKenzie AN; Neurath MF; Pflanz S; Wirtz S. 2013. Interleukin-33-dependent innate lymphoid cells mediate hepatic fibrosis. Immunity 39(2):357-71. [PubMed: 23954132]  [MGI Ref ID J:208227]

Mirmonsef P; Shelburne CP; Fitzhugh Yeatman C 2nd; Chong HJ; Ryan JJ. 1999. Inhibition of Kit expression by IL-4 and IL-10 in murine mast cells: role of STAT6 and phosphatidylinositol 3'-kinase. J Immunol 163(5):2530-9. [PubMed: 10452990]  [MGI Ref ID J:57096]

Mirosavljevic D; Quinn JM; Elliott J; Horwood NJ; Martin TJ; Gillespie MT. 2003. T-cells mediate an inhibitory effect of interleukin-4 on osteoclastogenesis. J Bone Miner Res 18(6):984-93. [PubMed: 12817750]  [MGI Ref ID J:111463]

Morris SC; Heidorn SM; Herbert DR; Perkins C; Hildeman DA; Khodoun MV; Finkelman FD. 2009. Endogenously produced IL-4 nonredundantly stimulates CD8+ T cell proliferation. J Immunol 182(3):1429-38. [PubMed: 19155490]  [MGI Ref ID J:144327]

Morrot A; Hafalla JC; Cockburn IA; Carvalho LH; Zavala F. 2005. IL-4 receptor expression on CD8+ T cells is required for the development of protective memory responses against liver stages of malaria parasites. J Exp Med 202(4):551-60. [PubMed: 16087712]  [MGI Ref ID J:100507]

Nagase H; Jones KM; Anderson CF; Noben-Trauth N. 2007. Despite increased CD4+Foxp3+ cells within the infection site, BALB/c IL-4 receptor-deficient mice reveal CD4+Foxp3-negative T cells as a source of IL-10 in Leishmania major susceptibility. J Immunol 179(4):2435-44. [PubMed: 17675505]  [MGI Ref ID J:151217]

Nemeth K; Keane-Myers A; Brown JM; Metcalfe DD; Gorham JD; Bundoc VG; Hodges MG; Jelinek I; Madala S; Karpati S; Mezey E. 2010. Bone marrow stromal cells use TGF-beta to suppress allergic responses in a mouse model of ragweed-induced asthma. Proc Natl Acad Sci U S A 107(12):5652-7. [PubMed: 20231466]  [MGI Ref ID J:158920]

Noben-Trauth N. 2000. Susceptibility to Leishmania major infection in the absence of IL-4. Immunol Lett 75(1):41-4. [PubMed: 11163865]  [MGI Ref ID J:66151]

Noben-Trauth N; Hu-Li J; Paul WE. 2000. Conventional, naive CD4+ T cells provide an initial source of IL-4 during Th2 differentiation. J Immunol 165(7):3620-5. [PubMed: 11034364]  [MGI Ref ID J:119587]

Noben-Trauth N; Lira R; Nagase H; Paul WE; Sacks DL. 2003. The relative contribution of IL-4 receptor signaling and IL-10 to susceptibility to Leishmania major. J Immunol 170(10):5152-8. [PubMed: 12734362]  [MGI Ref ID J:123822]

Noben-Trauth N; Paul WE; Sacks DL. 1999. IL-4- and IL-4 receptor-deficient BALB/c mice reveal differences in susceptibility to Leishmania major parasite substrains. J Immunol 162(10):6132-40. [PubMed: 10229856]  [MGI Ref ID J:55009]

Noben-Trauth N; Shultz LD; Brombacher F; Urban JF Jr; Gu H; Paul WE. 1997. An interleukin 4 (IL-4)-independent pathway for CD4+ T cell IL-4 production is revealed in IL-4 receptor-deficient mice. Proc Natl Acad Sci U S A 94(20):10838-43. [PubMed: 9380721]  [MGI Ref ID J:43656]

Okabe Y; Medzhitov R. 2014. Tissue-specific signals control reversible program of localization and functional polarization of macrophages. Cell 157(4):832-44. [PubMed: 24792964]  [MGI Ref ID J:214405]

Omori-Miyake M; Yamashita M; Tsunemi Y; Kawashima M; Yagi J. 2014. In Vitro Assessment of IL-4- or IL-13-Mediated Changes in the Structural Components of Keratinocytes in Mice and Humans. J Invest Dermatol 134(5):1342-50. [PubMed: 24280725]  [MGI Ref ID J:208078]

Ponomarev ED; Maresz K; Tan Y; Dittel BN. 2007. CNS-derived interleukin-4 is essential for the regulation of autoimmune inflammation and induces a state of alternative activation in microglial cells. J Neurosci 27(40):10714-21. [PubMed: 17913905]  [MGI Ref ID J:125605]

Potter MR; Noben-Trauth N; Weis JH; Teuscher C; Weis JJ. 2000. Interleukin-4 (IL-4) and IL-13 signaling pathways Do not regulate borrelia burgdorferi-induced arthritis in mice: IgG1 is not required for host control of tissue spirochetes Infect Immun 68(10):5603-9. [PubMed: 10992460]  [MGI Ref ID J:64838]

Radu DL; Noben-Trauth N; Hu-Li J; Paul WE; Bona CA. 2000. A targeted mutation in the IL-4Ralpha gene protects mice against autoimmune diabetes Proc Natl Acad Sci U S A 97(23):12700-4. [PubMed: 11050183]  [MGI Ref ID J:65807]

Rangachari M; Mauermann N; Marty RR; Dirnhofer S; Kurrer MO; Komnenovic V; Penninger JM; Eriksson U. 2006. T-bet negatively regulates autoimmune myocarditis by suppressing local production of interleukin 17. J Exp Med 203(8):2009-19. [PubMed: 16880257]  [MGI Ref ID J:124393]

Roth F; De La Fuente AC; Vella JL; Zoso A; Inverardi L; Serafini P. 2012. Aptamer-mediated blockade of IL4Ralpha triggers apoptosis of MDSCs and limits tumor progression. Cancer Res 72(6):1373-83. [PubMed: 22282665]  [MGI Ref ID J:184944]

Schneider D; Hong JY; Popova AP; Bowman ER; Linn MJ; McLean AM; Zhao Y; Sonstein J; Bentley JK; Weinberg JB; Lukacs NW; Curtis JL; Sajjan US; Hershenson MB. 2012. Neonatal rhinovirus infection induces mucous metaplasia and airways hyperresponsiveness. J Immunol 188(6):2894-904. [PubMed: 22331068]  [MGI Ref ID J:181841]

Sharkhuu T; Matthaei KI; Forbes E; Mahalingam S; Hogan SP; Hansbro PM; Foster PS. 2006. Mechanism of interleukin-25 (IL-17E)-induced pulmonary inflammation and airways hyper-reactivity. Clin Exp Allergy 36(12):1575-83. [PubMed: 17177681]  [MGI Ref ID J:135913]

Sharma A; Berga-Bolanos R; Sultana DA; Sen JM. 2013. IL-4 and IL-4 receptor expression is dispensable for the development and function of natural killer T cells. PLoS One 8(8):e71872. [PubMed: 23990998]  [MGI Ref ID J:205960]

Shirey KA; Cole LE; Keegan AD; Vogel SN. 2008. Francisella tularensis live vaccine strain induces macrophage alternative activation as a survival mechanism. J Immunol 181(6):4159-67. [PubMed: 18768873]  [MGI Ref ID J:139088]

Sinha P; Clements VK; Ostrand-Rosenberg S. 2005. Interleukin-13-regulated M2 macrophages in combination with myeloid suppressor cells block immune surveillance against metastasis. Cancer Res 65(24):11743-51. [PubMed: 16357187]  [MGI Ref ID J:104344]

Sintes J; Cuenca M; Romero X; Bastos R; Terhorst C; Angulo A; Engel P. 2013. Cutting Edge: Ly9 (CD229), a SLAM Family Receptor, Negatively Regulates the Development of Thymic Innate Memory-like CD8+ T and Invariant NKT Cells. J Immunol 190(1):21-6. [PubMed: 23225888]  [MGI Ref ID J:190814]

Spencer L; Shultz L; Rajan TV. 2001. Interleukin-4 receptor-stat6 signaling in murine infections with a tissue-dwelling nematode parasite. Infect Immun 69(12):7743-52. [PubMed: 11705956]  [MGI Ref ID J:73137]

Stephenson L; Johns MH; Woodward E; Mora AL; Boothby M. 2004. An IL-4R alpha allelic variant, I50, acts as a gain-of-function variant relative to V50 for Stat6, but not Th2 differentiation. J Immunol 173(7):4523-8. [PubMed: 15383584]  [MGI Ref ID J:93726]

Stephenson LM; Park DS; Mora AL; Goenka S; Boothby M. 2005. Sequence motifs in IL-4R alpha mediating cell-cycle progression of primary lymphocytes. J Immunol 175(8):5178-85. [PubMed: 16210622]  [MGI Ref ID J:119119]

Strait RT; Morris SC; Smiley K; Urban JF Jr; Finkelman FD. 2003. IL-4 exacerbates anaphylaxis. J Immunol 170(7):3835-42. [PubMed: 12646651]  [MGI Ref ID J:125442]

Swain SD; Han S; Harmsen A; Shampeny K; Harmsen AG. 2007. Pulmonary hypertension can be a sequela of prior pneumocystis pneumonia. Am J Pathol 171(3):790-9. [PubMed: 17640969]  [MGI Ref ID J:124302]

Swain SD; Meissner N; Han S; Harmsen A. 2011. Pneumocystis infection in an immunocompetent host can promote collateral sensitization to respiratory antigens. Infect Immun 79(5):1905-14. [PubMed: 21343358]  [MGI Ref ID J:171957]

Tachdjian R; Mathias C; Al Khatib S; Bryce PJ; Kim HS; Blaeser F; O'Connor BD; Rzymkiewicz D; Chen A; Holtzman MJ; Hershey GK; Garn H; Harb H; Renz H; Oettgen HC; Chatila TA. 2009. Pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma. J Exp Med 206(10):2191-204. [PubMed: 19770271]  [MGI Ref ID J:152376]

Terabe M; Khanna C; Bose S; Melchionda F; Mendoza A; Mackall CL; Helman LJ; Berzofsky JA. 2006. CD1d-restricted natural killer T cells can down-regulate tumor immunosurveillance independent of interleukin-4 receptor-signal transducer and activator of transcription 6 or transforming growth factor-beta. Cancer Res 66(7):3869-75. [PubMed: 16585215]  [MGI Ref ID J:108313]

Ueda N; Kuki H; Kamimura D; Sawa S; Seino K; Tashiro T; Fushuku K; Taniguchi M; Hirano T; Murakami M. 2006. CD1d-restricted NKT cell activation enhanced homeostatic proliferation of CD8+ T cells in a manner dependent on IL-4. Int Immunol 18(9):1397-404. [PubMed: 16914507]  [MGI Ref ID J:113387]

Urban JF Jr; Noben-Trauth N; Donaldson DD; Madden KB; Morris SC; Collins M; Finkelman FD. 1998. IL-13, IL-4Ralpha, and Stat6 are required for the expulsion of the gastrointestinal nematode parasite Nippostrongylus brasiliensis. Immunity 8(2):255-64. [PubMed: 9492006]  [MGI Ref ID J:110429]

Urban JF Jr; Noben-Trauth N; Schopf L; Madden KB; Finkelman FD. 2001. Cutting edge: IL-4 receptor expression by non-bone marrow-derived cells is required to expel gastrointestinal nematode parasites. J Immunol 167(11):6078-81. [PubMed: 11714764]  [MGI Ref ID J:119046]

Van den Bossche J; Bogaert P; van Hengel J; Guerin CJ; Berx G; Movahedi K; Van den Bergh R; Pereira-Fernandes A; Geuns JM; Pircher H; Dorny P; Grooten J; De Baetselier P; Van Ginderachter JA. 2009. Alternatively activated macrophages engage in homotypic and heterotypic interactions through IL-4 and polyamine-induced E-cadherin/catenin complexes. Blood 114(21):4664-74. [PubMed: 19726720]  [MGI Ref ID J:155503]

Vang KB; Yang J; Mahmud SA; Burchill MA; Vegoe AL; Farrar MA. 2008. IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell development. J Immunol 181(5):3285-90. [PubMed: 18714000]  [MGI Ref ID J:138950]

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Voehringer D; van Rooijen N; Locksley RM. 2007. Eosinophils develop in distinct stages and are recruited to peripheral sites by alternatively activated macrophages. J Leukoc Biol 81(6):1434-44. [PubMed: 17339609]  [MGI Ref ID J:122340]

Wang ML; Keilbaugh SA; Cash-Mason T; He XC; Li L; Wu GD. 2008. Immune-mediated signaling in intestinal goblet cells via PI3-kinase- and AKT-dependent pathways. Am J Physiol Gastrointest Liver Physiol 295(5):G1122-30. [PubMed: 18832447]  [MGI Ref ID J:143440]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Il4ratm1Sz  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Il4ratm1Sz x Homozygous for Il4ratm1Sz  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Il4ratm1Sz  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Il4ratm1Sz x Homozygous for Il4ratm1Sz  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000651 BALB/cJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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