Strain Name: |
FVB.Cg-Tg(CAG-EGFP)B5Nagy/J |
|---|---|
Stock Number: |
003516 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
FVB.Cg-Tg(ACTB-EGFP)B5Nagy/J (Changed: 08-MAY-08
) |
|
FVB.Cg-Tg(GFPU)5Nagy/J (Changed: 08-NOV-06
) | |
| Genes & Alleles | ACTB; GFP; Tg(CAG-EGFP)B5Nagy; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N6F15N1F3 (08-JAN-08) Strain Description
This transgenic strain expresses an Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. Mice, and cells derived from them, are distinguished from wildtype on the basis of fluorescence. The transgene is expressed in all nucleated embryonic tissues. Cells and tissues with increased hemoglobin content exhibit reduced fluorescence as development progresses. In newborn and adult mice, the entire organ system expresses EGFP. Though widespread, expression levels vary between different organs. This strain can be used as a source of fluorescently marked cells or tissues.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing an Enhanced Green Fluorescent Protein gene (Clontech) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells containing the construct were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females for germline transmission. The transgenic offspring were intercrossed to generate homozygotes. Mice derived from founder line B5/EGFP were sent to The Jackson Laboratory in 1998 and used to establish the colony on a mixed genetic background (see Stock No. 003115). From there, some mice were then backcrossed for more than 5 generations to FVB/NJ mice to establish this congenic strain.
Gene & Allele Details
| Allele Symbol | Tg(CAG-EGFP)B5Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion B5, Andras Nagy | ||
| Common Name(s) | B5/EGFP; Efgp; Tg (GFPU) 5Nagy; Tg(ACTB-EGFP)B5Nagy; Tg(GFPU)5Nagy; TgN(GFPU)5Nagy; | ||
| Mutation Made By | Andras Nagy, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | The transgene is expressed in all nucleated embryonic tissues, Newborn and adult mice exhibit expression unbiquitously, though the level of expression varies in different organs. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | In transgenic newborn and adult mice, the entire organ system expresses GFP. While transgene expression is ubiquitous, expression levels vary between organs. Cells and tissues with increased hemoglobin content exhibit reduced fluorescence as development progresses. | ||
| Molecular Note | The transgene carries the Green Fluorescent Protein driven by chicken beta actin promoter and CMV intermediate early enhancer. The construct was electroporated into R1 ES cells, for which the strain of origin is (129X1/SvJ x 129S1/Sv)F1-Kitl+.Random integration occurred on an unidentified autosome. The transgene is expressed in all nucleated embryonic tissues. [MGI Ref ID J:92926] | ||
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Tg(GFP) QPCR
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
003115 STOCK Tg(CAG-EGFP)B5Nagy/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
JAX Notes, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX Notes, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedHematological Research
Hemoglobin Defects
Research Tools
Cardiovascular Research
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesHadjantonakis AK; Gertsenstein M; Ikawa M; Okabe M; Nagy A. 1998. Generating green fluorescent mice by germline transmission of green fluorescent ES cells. Mech Dev 76(1-2):79-90. [PubMed: 9867352] [MGI Ref ID J:92926]
Price and Supply Information
| Strain Name: | FVB.Cg-Tg(CAG-EGFP)B5Nagy/J |
| Stock Number: | 003516 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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