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| These B5/EGFP transgenic mice have expression of enhanced green fluorescent protein (EGFP) directed to widespread tissues by the CMV-IE enhancer/chicken β-actin/rabbit β-globin hybrid promoter, and may be useful as a source of fluorescently marked cells or tissues. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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Description
Strain Information
Former Names FVB.Cg-Tg(ACTB-EGFP)B5Nagy/J (Changed: 08-MAY-08 ) FVB.Cg-Tg(GFPU)5Nagy/J (Changed: 08-NOV-06 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N6+2F4 (22-JAN-09)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
These B5/EGFP transgenic mice have expression of enhanced green fluorescent protein (EGFP) directed to widespread tissues by the CMV-IE enhancer/chicken β-actin/rabbit β-globin hybrid promoter. The transgene is expressed in all nucleated embryonic tissues. Transgenic mice, and cells derived from them, are distinguished from wildtype on the basis of fluorescence. In newborn and adult mice, the entire organ system expresses EGFP. Though widespread, expression levels vary between different organs. In addition, cells and tissues with increased hemoglobin content exhibit reduced fluorescence as development progresses. These B5/EGFP transgenic mice may be useful as a source of fluorescently marked cells or tissues.Animals homozygous for B5/EGFP transgene display increased incidence of lymphoma compared to animals hemizygous for this allele. Lymphoma in homozygous breeders has been observed as early as four months of age.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We may modify the strain description if necessary as published results become available.
Development
The B5/EGFP transgenic mice have expression of enhanced green fluorescent protein (EGFP) directed to widespread tissues by the CMV-IE enhancer/chicken β-actin/rabbit β-globin hybrid promoter. A transgenic was designed with an enhanced green fluorescent protein (EGFP) gene (Clontech) under the control of the a CMV-IE enhancer/chicken β-actin/rabbit β-globin hybrid promoter. The transgene was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells containing the construct were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females for germline transmission. The transgenic offspring were intercrossed to generate homozygotes. Mice derived from founder line B5/EGFP (mixed ICR and 129 genetic background) were sent to The Jackson Laboratory Repository in 1998 as Stock No. 003115). Upon arrival, some mice were then backcrossed to FVB/NJ inbred mice (Stock No. 001800) for more than five generations to establish this congenic strain (Stock No. 003516).
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
003115 STOCK Tg(CAG-EGFP)B5Nagy/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedHematological Research
Hemoglobin Defects
Research Tools
Cardiovascular Research
Developmental Biology Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-EGFP)B5Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion B5, Andras Nagy | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | B5/EGFP; Efgp; Tg (GFPU) 5Nagy; Tg(ACTB-EGFP)B5Nagy; Tg(GFPU)5Nagy; TgN(GFPU)5Nagy; actin-GFP; beta-actin:GFP; | ||
| Mutation Made By | Dr. Andras Nagy, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | The transgene is expressed in all nucleated embryonic tissues, Newborn and adult mice exhibit expression unbiquitously, though the level of expression varies in different organs. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Gene Symbol and Name | Tg(CAG-EGFP)B5Nagy, transgene insertion B5, Andras Nagy | ||
| Chromosome | UN | ||
| Gene Common Name(s) | B5/EGFP; Efgp; Tg (GFPU) 5Nagy; Tg(ACTB-EGFP)B5Nagy; Tg(GFPU)5Nagy; TgN(GFPU)5Nagy; beta-actin:GFP; transgene insertion 5, Andras Nagy; | ||
| General Note | In transgenic newborn and adult mice, the entire organ system expresses GFP. While transgene expression is ubiquitous, expression levels vary between organs. Cells and tissues with increased hemoglobin content exhibit reduced fluorescence as development progresses. | ||
| Molecular Note | The construct contains an enhanced green fluorescent protein gene driven by the CMV intermediate early enhancer coupled to the chicken beta actin promoter. The transgene integrated occurred on an unidentified autosome, and is expressed in all nucleated embryonic tissues. [MGI Ref ID J:92926] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hadjantonakis AK; Gertsenstein M; Ikawa M; Okabe M; Nagy A. 1998. Generating green fluorescent mice by germline transmission of green fluorescent ES cells. Mech Dev 76(1-2):79-90. [PubMed: 9867352] [MGI Ref ID J:92926]
Additional References
Nguyen-Ba-Charvet KT; Picard-Riera N; Tessier-Lavigne M; Baron-Van Evercooren A; Sotelo C; Chedotal A. 2004. Multiple roles for slits in the control of cell migration in the rostral migratory stream. J Neurosci 24(6):1497-506. [PubMed: 14960623] [MGI Ref ID J:90079]
Tg(CAG-EGFP)B5Nagy relatedAhn GO; Brown JM. 2008. Matrix metalloproteinase-9 is required for tumor vasculogenesis but not for angiogenesis: role of bone marrow-derived myelomonocytic cells. Cancer Cell 13(3):193-205. [PubMed: 18328424] [MGI Ref ID J:132946]
Beverdam A; Svingen T; Bagheri-Fam S; McClive P; Sinclair AH; Harley VR; Koopman P. 2010. Protein tyrosine kinase 2 beta (PTK2B), but not focal adhesion kinase (FAK), is expressed in a sexually dimorphic pattern in developing mouse gonads. Dev Dyn :. [PubMed: 20737507] [MGI Ref ID J:164222]
Bialecka M; Wilson V; Deschamps J. 2010. Cdx mutant axial progenitor cells are rescued by grafting to a wild type environment. Dev Biol 347(1):228-34. [PubMed: 20816799] [MGI Ref ID J:165819]
Biechele S; Cox BJ; Rossant J. 2011. Porcupine homolog is required for canonical Wnt signaling and gastrulation in mouse embryos. Dev Biol 355(2):275-85. [PubMed: 21554866] [MGI Ref ID J:173623]
Bielle F; Marcos-Mondejar P; Leyva-Diaz E; Lokmane L; Mire E; Mailhes C; Keita M; Garcia N; Tessier-Lavigne M; Garel S; Lopez-Bendito G. 2011. Emergent growth cone responses to combinations of Slit1 and Netrin 1 in thalamocortical axon topography. Curr Biol 21(20):1748-55. [PubMed: 22000108] [MGI Ref ID J:178526]
Brennan J; Karl J; Capel B. 2002. Divergent Vascular Mechanisms Downstream of Sry Establish the Arterial System in the XY Gonad. Dev Biol 244(2):418-28. [PubMed: 11944948] [MGI Ref ID J:76133]
Cao YA; Bachmann MH; Beilhack A; Yang Y; Tanaka M; Swijnenburg RJ; Reeves R; Taylor-Edwards C; Schulz S; Doyle TC; Fathman CG; Robbins RC; Herzenberg LA; Negrin RS; Contag CH. 2005. Molecular imaging using labeled donor tissues reveals patterns of engraftment, rejection, and survival in transplantation. Transplantation 80(1):134-9. [PubMed: 16003245] [MGI Ref ID J:137739]
Cavel O; Shomron O; Shabtay A; Vital J; Trejo-Leider L; Weizman N; Krelin Y; Fong Y; Wong RJ; Amit M; Gil Z. 2012. Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor. Cancer Res 72(22):5733-43. [PubMed: 22971345] [MGI Ref ID J:193214]
Chapman HA; Li X; Alexander JP; Brumwell A; Lorizio W; Tan K; Sonnenberg A; Wei Y; Vu TH. 2011. Integrin alpha6beta4 identifies an adult distal lung epithelial population with regenerative potential in mice. J Clin Invest 121(7):2855-62. [PubMed: 21701069] [MGI Ref ID J:175915]
Cho JH; Mao CA; Klein WH. 2012. Adult mice transplanted with embryonic retinal progenitor cells: new approach for repairing damaged optic nerves. Mol Vis 18:2658-72. [PubMed: 23170059] [MGI Ref ID J:192318]
Choi J; Curtis SJ; Roy DM; Flesken-Nikitin A; Nikitin AY. 2010. Local mesenchymal stem/progenitor cells are a preferential target for initiation of adult soft tissue sarcomas associated with p53 and Rb deficiency. Am J Pathol 177(5):2645-58. [PubMed: 20864684] [MGI Ref ID J:166254]
Cox BJ; Vollmer M; Tamplin O; Lu M; Biechele S; Gertsenstein M; van Campenhout C; Floss T; Kuhn R; Wurst W; Lickert H; Rossant J. 2010. Phenotypic annotation of the mouse X chromosome. Genome Res 20(8):1154-64. [PubMed: 20548051] [MGI Ref ID J:163340]
DeFalco T; Takahashi S; Capel B. 2011. Two distinct origins for Leydig cell progenitors in the fetal testis. Dev Biol 352(1):14-26. [PubMed: 21255566] [MGI Ref ID J:171480]
Direkze NC; Jeffery R; Hodivala-Dilke K; Hunt T; Playford RJ; Elia G; Poulsom R; Wright NA; Alison MR. 2006. Bone marrow-derived stromal cells express lineage-related messenger RNA species. Cancer Res 66(3):1265-9. [PubMed: 16452177] [MGI Ref ID J:106685]
Dolgachev VA; Ullenbruch MR; Lukacs NW; Phan SH. 2009. Role of stem cell factor and bone marrow-derived fibroblasts in airway remodeling. Am J Pathol 174(2):390-400. [PubMed: 19147822] [MGI Ref ID J:144184]
Flames N; Long JE; Garratt AN; Fischer TM; Gassmann M; Birchmeier C; Lai C; Rubenstein JL; Marin O. 2004. Short- and long-range attraction of cortical GABAergic interneurons by neuregulin-1. Neuron 44(2):251-61. [PubMed: 15473965] [MGI Ref ID J:130617]
Galvao RP; Garcia-Verdugo JM; Alvarez-Buylla A. 2008. Brain-derived neurotrophic factor signaling does not stimulate subventricular zone neurogenesis in adult mice and rats. J Neurosci 28(50):13368-83. [PubMed: 19074010] [MGI Ref ID J:143055]
Gao D; Joshi N; Choi H; Ryu S; Hahn M; Catena R; Sadik H; Argani P; Wagner P; Vahdat LT; Port JL; Stiles B; Sukumar S; Altorki NK; Rafii S; Mittal V. 2012. Myeloid progenitor cells in the premetastatic lung promote metastases by inducing mesenchymal to epithelial transition. Cancer Res 72(6):1384-94. [PubMed: 22282653] [MGI Ref ID J:184945]
Grimaldi P; Parras C; Guillemot F; Rossi F; Wassef M. 2009. Origins and control of the differentiation of inhibitory interneurons and glia in the cerebellum. Dev Biol 328(2):422-33. [PubMed: 19217896] [MGI Ref ID J:149261]
Hadjantonakis AK; Nagy A. 2000. FACS for the isolation of individual cells from transgenic mice harboring a fluorescent protein reporter Genesis 27(3):95-8. [PubMed: 10951501] [MGI Ref ID J:64336]
Kinder SJ; Tsang TE; Wakamiya M; Sasaki H; Behringer RR; Nagy A; Tam PP. 2001. The organizer of the mouse gastrula is composed of a dynamic population of progenitor cells for the axial mesoderm. Development 128(18):3623-34. [PubMed: 11566865] [MGI Ref ID J:71995]
Ko DC; Milenkovic L; Beier SM; Manuel H; Buchanan J; Scott MP. 2005. Cell-autonomous death of cerebellar purkinje neurons with autophagy in niemann-pick type C disease. PLoS Genet 1(1):e7. [PubMed: 16103921] [MGI Ref ID J:100117]
Kruithof-de Julio M; Alvarez MJ; Galli A; Chu J; Price SM; Califano A; Shen MM. 2011. Regulation of extra-embryonic endoderm stem cell differentiation by Nodal and Cripto signaling. Development 138(18):3885-95. [PubMed: 21862554] [MGI Ref ID J:176246]
Lakhina V; Falnikar A; Bhatnagar L; Tole S. 2007. Early thalamocortical tract guidance and topographic sorting of thalamic projections requires LIM-homeodomain gene Lhx2. Dev Biol 306(2):703-13. [PubMed: 17493606] [MGI Ref ID J:122567]
Li L; Wang L; Li L; Wang Z; Ho Y; McDonald T; Holyoake TL; Chen W; Bhatia R. 2012. Activation of p53 by SIRT1 Inhibition Enhances Elimination of CML Leukemia Stem Cells in Combination with Imatinib. Cancer Cell 21(2):266-81. [PubMed: 22340598] [MGI Ref ID J:181458]
Li XM; Hu Z; Zafar AB; Jorgensen ML; Bungert J; Slayton W. 2010. Intrinsic and extrinsic effects of mafG deficiency on hematopoietic recovery following bone marrow transplant. Exp Hematol 38(12):1251-60. [PubMed: 20813153] [MGI Ref ID J:169721]
Lopez-Bendito G; Sanchez-Alcaniz JA; Pla R; Borrell V; Pico E; Valdeolmillos M; Marin O. 2008. Chemokine signaling controls intracortical migration and final distribution of GABAergic interneurons. J Neurosci 28(7):1613-24. [PubMed: 18272682] [MGI Ref ID J:132219]
Lu Y; Giver CR; Sharma A; Li JM; Darlak KA; Owens LM; Roback JD; Galipeau J; Waller EK. 2012. IFN-gamma and indoleamine 2,3-dioxygenase signaling between donor dendritic cells and T cells regulates graft versus host and graft versus leukemia activity. Blood 119(4):1075-85. [PubMed: 22130799] [MGI Ref ID J:181750]
Mire E; Mezzera C; Leyva-Diaz E; Paternain AV; Squarzoni P; Bluy L; Castillo-Paterna M; Lopez MJ; Peregrin S; Tessier-Lavigne M; Garel S; Galceran J; Lerma J; Lopez-Bendito G. 2012. Spontaneous activity regulates Robo1 transcription to mediate a switch in thalamocortical axon growth. Nat Neurosci 15(8):1134-43. [PubMed: 22772332] [MGI Ref ID J:190078]
Nair KS; Hanson SM; Mendez A; Gurevich EV; Kennedy MJ; Shestopalov VI; Vishnivetskiy SA; Chen J; Hurley JB; Gurevich VV; Slepak VZ. 2005. Light-dependent redistribution of arrestin in vertebrate rods is an energy-independent process governed by protein-protein interactions. Neuron 46(4):555-67. [PubMed: 15944125] [MGI Ref ID J:99855]
Nguyen-Ba-Charvet KT; Plump AS; Tessier-Lavigne M; Chedotal A. 2002. Slit1 and slit2 proteins control the development of the lateral olfactory tract. J Neurosci 22(13):5473-80. [PubMed: 12097499] [MGI Ref ID J:109226]
Nicol X; Voyatzis S; Muzerelle A; Narboux-Neme N; Sudhof TC; Miles R; Gaspar P. 2007. cAMP oscillations and retinal activity are permissive for ephrin signaling during the establishment of the retinotopic map. Nat Neurosci 10(3):340-7. [PubMed: 17259982] [MGI Ref ID J:120760]
Nieoullon V; Belvindrah R; Rougon G; Chazal G. 2005. mCD24 regulates proliferation of neuronal committed precursors in the subventricular zone. Mol Cell Neurosci 28(3):462-74. [PubMed: 15737737] [MGI Ref ID J:125133]
Niquille M; Garel S; Mann F; Hornung JP; Otsmane B; Chevalley S; Parras C; Guillemot F; Gaspar P; Yanagawa Y; Lebrand C. 2009. Transient neuronal populations are required to guide callosal axons: a role for semaphorin 3C. PLoS Biol 7(10):e1000230. [PubMed: 19859539] [MGI Ref ID J:154587]
O'Brien MJ; Pendola JK; Eppig JJ. 2003. A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence. Biol Reprod 68(5):1682-6. [PubMed: 12606400] [MGI Ref ID J:92927]
Pfister S; Jones VJ; Power M; Truisi GL; Khoo PL; Steiner KA; Kanai-Azuma M; Kanai Y; Tam PP; Loebel DA. 2011. Sox17-dependent gene expression and early heart and gut development in Sox17-deficient mouse embryos. Int J Dev Biol 55(1):45-58. [PubMed: 21305474] [MGI Ref ID J:170493]
Pla R; Borrell V; Flames N; Marin O. 2006. Layer acquisition by cortical GABAergic interneurons is independent of Reelin signaling. J Neurosci 26(26):6924-34. [PubMed: 16807322] [MGI Ref ID J:110037]
Ralston A; Rossant J. 2008. Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo. Dev Biol 313(2):614-29. [PubMed: 18067887] [MGI Ref ID J:130330]
Sandahl M; Hunter DM; Strunk KE; Earp HS; Cook RS. 2010. Epithelial cell-directed efferocytosis in the post-partum mammary gland is necessary for tissue homeostasis and future lactation. BMC Dev Biol 10:122. [PubMed: 21192804] [MGI Ref ID J:167986]
Somervaille TC; Cleary ML. 2006. Identification and characterization of leukemia stem cells in murine MLL-AF9 acute myeloid leukemia. Cancer Cell 10(4):257-68. [PubMed: 17045204] [MGI Ref ID J:115010]
Southwell DG; Paredes MF; Galvao RP; Jones DL; Froemke RC; Sebe JY; Alfaro-Cervello C; Tang Y; Garcia-Verdugo JM; Rubenstein JL; Baraban SC; Alvarez-Buylla A. 2012. Intrinsically determined cell death of developing cortical interneurons. Nature 491(7422):109-13. [PubMed: 23041929] [MGI Ref ID J:189225]
Tam PP; Khoo PL; Wong N; Tsang TE; Behringer RR. 2004. Regionalization of cell fates and cell movement in the endoderm of the mouse gastrula and the impact of loss of Lhx1(Lim1) function. Dev Biol 274(1):171-87. [PubMed: 15355796] [MGI Ref ID J:93592]
Templeton SP; Perlman S. 2008. Role of IFN-gamma responsiveness in CD8 T cell-mediated viral clearance and demyelination in coronavirus-infected mice. J Neuroimmunol 194(1-2):18-26. [PubMed: 18082272] [MGI Ref ID J:131907]
Ugarte G; Cappellari O; Perani L; Pistocchi A; Cossu G. 2012. Noggin recruits mesoderm progenitors from the dorsal aorta to a skeletal myogenic fate. Dev Biol 365(1):91-100. [PubMed: 22370001] [MGI Ref ID J:184923]
Vasiliev O; Rhodes SJ; Beebe DC. 2007. Identification and expression of Hop, an atypical homeobox gene expressed late in lens fiber cell terminal differentiation. Mol Vis 13:114-24. [PubMed: 17277742] [MGI Ref ID J:141592]
Vernochet C; Caucheteux SM; Kanellopoulos-Langevin C. 2007. Bi-directional cell trafficking between mother and fetus in mouse placenta. Placenta 28(7):639-49. [PubMed: 17116327] [MGI Ref ID J:130118]
Vintersten K; Monetti C; Gertsenstein M; Zhang P; Laszlo L; Biechele S; Nagy A. 2004. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis 40(4):241-6. [PubMed: 15593332] [MGI Ref ID J:109837]
Virard I; Coquillat D; Bancila M; Kaing S; Durbec P. 2006. Oligodendrocyte precursor cells generate pituicytes in vivo during neurohypophysis development. Glia 53(3):294-303. [PubMed: 16265670] [MGI Ref ID J:156148]
Voisin L; Saba-El-Leil MK; Julien C; Fremin C; Meloche S. 2010. Genetic demonstration of a redundant role of extracellular signal-regulated kinase 1 (ERK1) and ERK2 mitogen-activated protein kinases in promoting fibroblast proliferation. Mol Cell Biol 30(12):2918-32. [PubMed: 20368360] [MGI Ref ID J:162580]
Walz C; Jungling K; Lessmann V; Gottmann K. 2006. Presynaptic plasticity in an immature neocortical network requires NMDA receptor activation and BDNF release. J Neurophysiol 96(6):3512-6. [PubMed: 17110740] [MGI Ref ID J:135948]
Warr N; Siggers P; Bogani D; Brixey R; Pastorelli L; Yates L; Dean CH; Wells S; Satoh W; Shimono A; Greenfield A. 2009. Sfrp1 and Sfrp2 are required for normal male sexual development in mice. Dev Biol 326(2):273-84. [PubMed: 19100252] [MGI Ref ID J:147206]
Watson ED; Geary-Joo C; Hughes M; Cross JC. 2007. The Mrj co-chaperone mediates keratin turnover and prevents the formation of toxic inclusion bodies in trophoblast cells of the placenta. Development 134(9):1809-17. [PubMed: 17409114] [MGI Ref ID J:121264]
Xu Q; Cobos I; De La Cruz E; Rubenstein JL; Anderson SA. 2004. Origins of cortical interneuron subtypes. J Neurosci 24(11):2612-22. [PubMed: 15028753] [MGI Ref ID J:97012]
Zhang B; Ho YW; Huang Q; Maeda T; Lin A; Lee SU; Hair A; Holyoake TL; Huettner C; Bhatia R. 2012. Altered microenvironmental regulation of leukemic and normal stem cells in chronic myelogenous leukemia. Cancer Cell 21(4):577-92. [PubMed: 22516264] [MGI Ref ID J:189328]
Zhang B; Strauss AC; Chu S; Li M; Ho Y; Shiang KD; Snyder DS; Huettner CS; Shultz L; Holyoake T; Bhatia R. 2010. Effective targeting of quiescent chronic myelogenous leukemia stem cells by histone deacetylase inhibitors in combination with imatinib mesylate. Cancer Cell 17(5):427-42. [PubMed: 20478526] [MGI Ref ID J:160523]
Zhang W; Zhao J; Chen L; Urbanowicz MM; Nagasaki T. 2008. Abnormal epithelial homeostasis in the cornea of mice with a destrin deletion. Mol Vis 14:1929-39. [PubMed: 18958303] [MGI Ref ID J:144053]
Zhang Y; Daquinag A; Traktuev DO; Amaya-Manzanares F; Simmons PJ; March KL; Pasqualini R; Arap W; Kolonin MG. 2009. White adipose tissue cells are recruited by experimental tumors and promote cancer progression in mouse models. Cancer Res 69(12):5259-66. [PubMed: 19491274] [MGI Ref ID J:149658]
Zhao J; Mo V; Nagasaki T. 2009. Distribution of label-retaining cells in the limbal epithelium of a mouse eye. J Histochem Cytochem 57(2):177-85. [PubMed: 19001638] [MGI Ref ID J:146975]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Homozygous for Tg(CAG-EGFP)B5Nagy
Price per Pair (US dollars $) Pair Genotype $354.00 Homozygous for Tg(CAG-EGFP)B5Nagy x Homozygous for Tg(CAG-EGFP)B5Nagy Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Homozygous for Tg(CAG-EGFP)B5Nagy
Price per Pair (US dollars $) Pair Genotype $460.20 Homozygous for Tg(CAG-EGFP)B5Nagy x Homozygous for Tg(CAG-EGFP)B5Nagy Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.