Strain Name:

B6;129S6-Lrp8tm1Her/J

Stock Number:

003524

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas

Appearance
black
Related Genotype: a/a

Description
Mutant mice have a targeted mutation of the apolipoprotein E receptor Lrp8 (also called Apoer2). Homozygotes are viable with no gross morphological abnormalities. Homozygous males have reduced fertility, while females are not affected. Using C-terminal specific antibodies, no endogenous protein is detected in the brains of homozygotes. Homozygous mice have a smaller and less foliated cerebellum with various hippocampus defects in granule cell positioning, cortical neuron migration, granule cell laminar organization, commissural fiber distribution, CA1 microtubule-associated protein 2 (MAP-2) distribution, and long term potentiation (LTP). Mutant mice show contextual fear conditioning deficits. These mice may be useful in studies of brain development, neuronal cytoarchitecture, Reelin signaling pathways, NMDA receptor activity, lipoprotein receptors, synaptic plasticity and learning, schizophrenia, and neurodegenerative disorders such as Alzheimer's disease.

Development
A targeting vector was designed to replace exons 17 and 18 of the endogenous gene with a pol2neo cassette. These two exons encode most of the membrane-spanning segment and a portion of the cytoplasmic tail. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Mutant mice were maintained on a mixed C57BL/6 and 129S6 background before arriving at The Jackson Laboratory.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Myocardial Infarction, Susceptibility to   (LRP8)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Lrp8tm1Her/Lrp8tm1Her

        involves: 129S6/SvEvTac * C57BL/6J
  • nervous system phenotype
  • abnormal lateral ventricle morphology
    • neuroblasts invade the lateral ventricle   (MGI Ref ID J:121846)
  • abnormal postnatal subventricular zone morphology
    • some neuroblasts accumulate in the subventricular zone   (MGI Ref ID J:121846)
  • abnormal rostral migratory stream morphology
    • rostral migratory streaming is only slightly disrupted with the accumulation of some neuroblasts in the subventricular zone   (MGI Ref ID J:121846)

Lrp8tm1Her/Lrp8tm1Her

        involves: 129S6/SvEvTac * C57BL/6
  • nervous system phenotype
  • abnormal cerebral cortex morphology
    • cortical layer abnormalities, with some laminar dispersion of upper layer neurons   (MGI Ref ID J:129338)
  • abnormal hippocampus pyramidal cell layer
    • mutants exhibit a modest split of the pyramidal layer in area CA1 and CA3   (MGI Ref ID J:129338)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Lrp8tm1Her/Lrp8tm1Her

        involves: 129S6/SvEvTac
  • reproductive system phenotype
  • abnormal sperm physiology
    • mutant spermatozoa from the cauda epididymidis fail to regulate the intracellular osmotic pressure ("cell volume decrease")   (MGI Ref ID J:84102)
    • as a result, sperm fail to counteract water influx when released into hypo-osmotic conditions, causing cell swelling and coiling of the sperm tail   (MGI Ref ID J:84102)
    • asthenozoospermia
      • the % of motile mutant sperm is reduced to half the wild-type control value   (MGI Ref ID J:84102)
      • although curvilinear velocity and the amplitude of lateral head displacement remain normal, all other sperm analysis parameters are significantly reduced indicating ineffective forward progression   (MGI Ref ID J:84102)
  • abnormal spermatogenesis
    • male homozygotes display abnormal sperm maturation in the proximal epididymis   (MGI Ref ID J:84102)
    • however, mutant testicular and epididymal structures exhibit normal development, and spermatozoa descending from the seminiferous tubules of the testis into the initial segment of the epididymis are morphologically normal   (MGI Ref ID J:84102)
    • abnormal sperm flagellum morphology
      • after exit from the initial epididymal segment, distinct tail abnormalities are increasingly observed during transit of sperm through the epididymal duct   (MGI Ref ID J:84102)
      • abnormal sperm midpiece morphology
        • some sperm mid-pieces show abnormal kinking and fraying of axonemal structures   (MGI Ref ID J:84102)
        • abnormal sperm mitochondrial sheath morphology
          • unlike wild-type, most mutant spermatozoa exhibit a highly irregular mitochondrial helix in mid-piece sections of the sperm tail   (MGI Ref ID J:84102)
      • coiled sperm flagellum
        • ~51% of mutant spermatozoa from the cauda epididymidis display coiling of the tail with a variable degree of bending   (MGI Ref ID J:84102)
        • ~49% show normal straight tail morphology   (MGI Ref ID J:84102)
      • hairpin sperm flagellum
        • 43% of mutant spermatozoa from the cauda epididymidis exhibit hairpin structures (180 degrees)   (MGI Ref ID J:84102)
        • hairpin morphology is completely reverted upon incubation in buffer containing mild detergents (0.1% Triton X-100), indicating cell volume dysregulation   (MGI Ref ID J:84102)
      • kinked sperm flagellum
        • 8% of mutant spermatozoa from the cauda epididymidis show slight angulations (<90 degrees)   (MGI Ref ID J:84102)
  • male infertility   (MGI Ref ID J:55691)
    • male infertility is associated with reduced expression of a sperm maturation protein (GPX4) in the epididymis   (MGI Ref ID J:84102)
  • behavior/neurological phenotype
  • abnormal contextual conditioning behavior
    • contextual fear-conditioned learning deficits   (MGI Ref ID J:55691)
  • abnormal spatial learning
    • homozygotes spent less time in the training quadrant and crossed the former platform position less often in probe trials compared to wild-type mice   (MGI Ref ID J:100988)
  • nervous system phenotype
  • abnormal Purkinje cell morphology
    • abnormal aggregates of Purkinje cells and cortical neurons   (MGI Ref ID J:55691)
  • abnormal brain development
    • cortical layering disrupted, with neurons packed into tight horizontal layers   (MGI Ref ID J:55691)
    • abnormal cerebellar foliation
      • less foliated than wild-type   (MGI Ref ID J:55691)
  • abnormal cerebral cortex morphology
    • at P21, markers for cortical layer 6 and subplate neurons have broadened expression, indicating that neurons are infiltrating layers closer to the cortical surface   (MGI Ref ID J:105699)
  • abnormal dentate gyrus morphology
    • neurons in the dentate gyrus were very loosely packed   (MGI Ref ID J:55691)
    • ectopic calbindin-positive cells are located mainly in the polymorph layer between the densely packed granule layers   (MGI Ref ID J:105699)
  • abnormal hippocampus layer morphology
    • ectopic calbindin-positive cells are located mainly in the polymorph layer between the densely packed granule layers   (MGI Ref ID J:105699)
  • abnormal neuronal migration
    • corticospinal neurons are mislocalized to layers 4 and 6 of cerebral cortex   (MGI Ref ID J:117956)
  • small cerebellum   (MGI Ref ID J:55691)
  • cellular phenotype
  • abnormal cell death
    • in mutants, corticospinal neurons are resistant to lesion-induced cell death whereas ~40% of wild-type CSN die after CSN deafferentation at 1 month of age; at 4 months, survival ratio is similar   (MGI Ref ID J:117956)
  • abnormal neuronal migration
    • corticospinal neurons are mislocalized to layers 4 and 6 of cerebral cortex   (MGI Ref ID J:117956)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Lrp8tm1Her related

Developmental Biology Research
Defects in Extracellular Matrix Molecules
Neurodevelopmental Defects

Neurobiology Research
Cerebellar Defects
Cortical Defects
Neurodevelopmental Defects
Receptor Defects
Tremor Defects

Research Tools
Neurobiology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Lrp8tm1Her
Allele Name targeted mutation 1, Joachim Herz
Allele Type Targeted (knock-out)
Common Name(s) ApoER2-; Lp8tm1Her;
Mutation Made ByDr. Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas
Strain of Origin129S6/SvEvTac
ES Cell Line NameSM1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Lrp8, low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
Chromosome 4
Gene Common Name(s) 4932703M08Rik; AA921429; AI848122; APOER2; HSZ75190; LRP-8; Lr8b; MCI1; RIKEN cDNA 4932703M08 gene; cDNA sequence AA921429; expressed sequence AI848122; low density lipoprotein receptor relative, 8 ligand binding repeats, brain;
Molecular Note A neomycin resistance cassette replaced exons 17 and 18, which encode most of the membrane spanning segment and part of the cytoplasmic tail. [MGI Ref ID J:55691]

Genotyping

Genotyping Information

Genotyping Protocols

Lrp8tm1Her, Melt Curve Analysis
Lrp8tm1Her, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Trommsdorff M; Gotthardt M; Hiesberger T; Shelton J; Stockinger W ; Nimpf J ; Hammer RE ; Richardson JA ; Herz J. 1999. Reeler/Disabled-like disruption of neuronal migration in knockout mice lacking the VLDL receptor and ApoE receptor 2. Cell 97(6):689-701. [PubMed: 10380922]  [MGI Ref ID J:55691]

Additional References

Drakew A; Deller T; Heimrich B; Gebhardt C; Del Turco D; Tielsch A; Forster E; Herz J; Frotscher M. 2002. Dentate granule cells in reeler mutants and VLDLR and ApoER2 knockout mice. Exp Neurol 176(1):12-24. [PubMed: 12093079]  [MGI Ref ID J:78001]

Gebhardt C; Del Turco D; Drakew A; Tielsch A; Herz J; Frotscher M; Deller T. 2002. Abnormal positioning of granule cells alters afferent fiber distribution in the mouse fascia dentata: Morphologic evidence from reeler, apolipoprotein E receptor 2-, and very low density lipoprotein receptor knockout mice. J Comp Neurol 445(3):278-92. [PubMed: 11920707]  [MGI Ref ID J:75080]

Weiss KH; Johanssen C; Tielsch A; Herz J; Deller T; Frotscher M; Forster E. 2003. Malformation of the radial glial scaffold in the dentate gyrus of reeler mice, scrambler mice, and ApoER2/VLDLR-deficient mice. J Comp Neurol 460(1):56-65. [PubMed: 12687696]  [MGI Ref ID J:83151]

Lrp8tm1Her related

Akopians AL; Babayan AH; Beffert U; Herz J; Basbaum AI; Phelps PE. 2008. Contribution of the Reelin signaling pathways to nociceptive processing. Eur J Neurosci 27(3):523-37. [PubMed: 18279306]  [MGI Ref ID J:132269]

Andersen OM; Yeung CH; Vorum H; Wellner M; Andreassen TK; Erdmann B; Mueller EC; Herz J; Otto A; Cooper TG; Willnow TE. 2003. Essential role of the apolipoprotein E receptor-2 in sperm development. J Biol Chem 278(26):23989-95. [PubMed: 12695510]  [MGI Ref ID J:84102]

Andrade N; Komnenovic V; Blake SM; Jossin Y; Howell B; Goffinet A; Schneider WJ; Nimpf J. 2007. ApoER2/VLDL receptor and Dab1 in the rostral migratory stream function in postnatal neuronal migration independently of Reelin. Proc Natl Acad Sci U S A 104(20):8508-13. [PubMed: 17494763]  [MGI Ref ID J:121846]

Assadi AH; Zhang G; Beffert U; McNeil RS; Renfro AL; Niu S; Quattrocchi CC; Antalffy BA; Sheldon M; Armstrong DD; Wynshaw-Boris A; Herz J; D'Arcangelo G; Clark GD. 2003. Interaction of reelin signaling and Lis1 in brain development. Nat Genet 35(3):270-6. [PubMed: 14578885]  [MGI Ref ID J:86398]

Barr AM; Fish KN; Markou A. 2007. The reelin receptors VLDLR and ApoER2 regulate sensorimotor gating in mice. Neuropharmacology 52(4):1114-23. [PubMed: 17261317]  [MGI Ref ID J:124495]

Beffert U; Durudas A; Weeber EJ; Stolt PC; Giehl KM; Sweatt JD; Hammer RE; Herz J. 2006. Functional dissection of Reelin signaling by site-directed disruption of Disabled-1 adaptor binding to apolipoprotein E receptor 2: distinct roles in development and synaptic plasticity. J Neurosci 26(7):2041-52. [PubMed: 16481437]  [MGI Ref ID J:105699]

Beffert U; Nematollah Farsian F; Masiulis I; Hammer RE; Yoon SO; Giehl KM; Herz J. 2006. ApoE receptor 2 controls neuronal survival in the adult brain. Curr Biol 16(24):2446-52. [PubMed: 17174920]  [MGI Ref ID J:117956]

Beffert U; Weeber EJ; Morfini G; Ko J; Brady ST; Tsai LH; Sweatt JD; Herz J. 2004. Reelin and cyclin-dependent kinase 5-dependent signals cooperate in regulating neuronal migration and synaptic transmission. J Neurosci 24(8):1897-906. [PubMed: 14985430]  [MGI Ref ID J:90122]

Blake SM; Strasser V; Andrade N; Duit S; Hofbauer R; Schneider WJ; Nimpf J. 2008. Thrombospondin-1 binds to ApoER2 and VLDL receptor and functions in postnatal neuronal migration. EMBO J 27(22):3069-80. [PubMed: 18946489]  [MGI Ref ID J:143787]

Burk RF; Hill KE; Olson GE; Weeber EJ; Motley AK; Winfrey VP; Austin LM. 2007. Deletion of apolipoprotein E receptor-2 in mice lowers brain selenium and causes severe neurological dysfunction and death when a low-selenium diet is fed. J Neurosci 27(23):6207-11. [PubMed: 17553992]  [MGI Ref ID J:121972]

Cariboni A; Rakic S; Liapi A; Maggi R; Goffinet A; Parnavelas JG. 2005. Reelin provides an inhibitory signal in the migration of gonadotropin-releasing hormone neurons. Development 132(21):4709-18. [PubMed: 16207762]  [MGI Ref ID J:102848]

Drakew A; Deller T; Heimrich B; Gebhardt C; Del Turco D; Tielsch A; Forster E; Herz J; Frotscher M. 2002. Dentate granule cells in reeler mutants and VLDLR and ApoER2 knockout mice. Exp Neurol 176(1):12-24. [PubMed: 12093079]  [MGI Ref ID J:78001]

Dumanis SB; Cha HJ; Song JM; Trotter JH; Spitzer M; Lee JY; Weeber EJ; Turner RS; Pak DT; Rebeck GW; Hoe HS. 2011. ApoE receptor 2 regulates synapse and dendritic spine formation. PLoS One 6(2):e17203. [PubMed: 21347244]  [MGI Ref ID J:171098]

Fish KN; Krucker T. 2008. Functional consequences of hippocampal neuronal ectopia in the apolipoprotein E receptor-2 knockout mouse. Neurobiol Dis 32(3):391-401. [PubMed: 18778775]  [MGI Ref ID J:142541]

Gebhardt C; Del Turco D; Drakew A; Tielsch A; Herz J; Frotscher M; Deller T. 2002. Abnormal positioning of granule cells alters afferent fiber distribution in the mouse fascia dentata: Morphologic evidence from reeler, apolipoprotein E receptor 2-, and very low density lipoprotein receptor knockout mice. J Comp Neurol 445(3):278-92. [PubMed: 11920707]  [MGI Ref ID J:75080]

Hack I; Hellwig S; Junghans D; Brunne B; Bock HH; Zhao S; Frotscher M. 2007. Divergent roles of ApoER2 and Vldlr in the migration of cortical neurons. Development 134(21):3883-91. [PubMed: 17913789]  [MGI Ref ID J:126335]

Hashimoto-Torii K; Torii M; Sarkisian MR; Bartley CM; Shen J; Radtke F; Gridley T; Sestan N; Rakic P. 2008. Interaction between Reelin and Notch signaling regulates neuronal migration in the cerebral cortex. Neuron 60(2):273-84. [PubMed: 18957219]  [MGI Ref ID J:144065]

Jossin Y; Gui L; Goffinet AM. 2007. Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons. J Neurosci 27(16):4243-52. [PubMed: 17442808]  [MGI Ref ID J:121108]

Kruger MT; Zhao S; Chai X; Brunne B; Bouche E; Bock HH; Frotscher M. 2010. Role for Reelin-induced cofilin phosphorylation in the assembly of sympathetic preganglionic neurons in the murine intermediolateral column. Eur J Neurosci 32(10):1611-7. [PubMed: 21039973]  [MGI Ref ID J:169495]

Larouche M; Beffert U; Herz J; Hawkes R. 2008. The reelin receptors apoer2 and vldlr coordinate the patterning of purkinje cell topography in the developing mouse cerebellum. PLoS ONE 3(2):e1653. [PubMed: 18301736]  [MGI Ref ID J:132885]

Leemhuis J; Bouche E; Frotscher M; Henle F; Hein L; Herz J; Meyer DK; Pichler M; Roth G; Schwan C; Bock HH. 2010. Reelin signals through apolipoprotein E receptor 2 and Cdc42 to increase growth cone motility and filopodia formation. J Neurosci 30(44):14759-72. [PubMed: 21048135]  [MGI Ref ID J:166701]

Masiulis I; Quill TA; Burk RF; Herz J. 2009. Differential functions of the Apoer2 intracellular domain in selenium uptake and cell signaling. Biol Chem 390(1):67-73. [PubMed: 19007311]  [MGI Ref ID J:153933]

Olson GE; Winfrey VP; Hill KE; Burk RF. 2008. Megalin mediates selenoprotein P uptake by kidney proximal tubule epithelial cells. J Biol Chem 283(11):6854-60. [PubMed: 18174160]  [MGI Ref ID J:133662]

Olson GE; Winfrey VP; Nagdas SK; Hill KE; Burk RF. 2007. Apolipoprotein E receptor-2 (ApoER2) mediates selenium uptake from selenoprotein P by the mouse testis. J Biol Chem 282(16):12290-7. [PubMed: 17314095]  [MGI Ref ID J:121140]

Petit-Turcotte C; Aumont N; Beffert U; Dea D; Herz J; Poirier J. 2005. The apoE receptor apoER2 is involved in the maintenance of efficient synaptic plasticity. Neurobiol Aging 26(2):195-206. [PubMed: 15582748]  [MGI Ref ID J:101963]

Quinn KL; Henriques M; Tabuchi A; Han B; Yang H; Cheng WE; Tole S; Yu H; Luo A; Charbonney E; Tullis E; Lazarus A; Robinson LA; Ni H; Peterson BR; Kuebler WM; Slutsky AS; Zhang H. 2011. Human neutrophil peptides mediate endothelial-monocyte interaction, foam cell formation, and platelet activation. Arterioscler Thromb Vasc Biol 31(9):2070-9. [PubMed: 21817096]  [MGI Ref ID J:191841]

Ramesh S; Morrell CN; Tarango C; Thomas GD; Yuhanna IS; Girardi G; Herz J; Urbanus RT; de Groot PG; Thorpe PE; Salmon JE; Shaul PW; Mineo C. 2011. Antiphospholipid antibodies promote leukocyte-endothelial cell adhesion and thrombosis in mice by antagonizing eNOS via beta2GPI and apoER2. J Clin Invest 121(1):120-31. [PubMed: 21123944]  [MGI Ref ID J:171855]

Romay-Penabad Z; Aguilar-Valenzuela R; Urbanus RT; Derksen RH; Pennings MT; Papalardo E; Shilagard T; Vargas G; Hwang Y; de Groot PG; Pierangeli SS. 2011. Apolipoprotein E receptor 2 is involved in the thrombotic complications in a murine model of the antiphospholipid syndrome. Blood 117(4):1408-14. [PubMed: 21119114]  [MGI Ref ID J:168586]

Rossel M; Loulier K; Feuillet C; Alonso S; Carroll P. 2005. Reelin signaling is necessary for a specific step in the migration of hindbrain efferent neurons. Development 132(6):1175-85. [PubMed: 15703280]  [MGI Ref ID J:97218]

Senturk A; Pfennig S; Weiss A; Burk K; Acker-Palmer A. 2011. Ephrin Bs are essential components of the Reelin pathway to regulate neuronal migration. Nature 472(7343):356-60. [PubMed: 21460838]  [MGI Ref ID J:171375]

Su J; Klemm MA; Josephson AM; Fox MA. 2013. Contributions of VLDLR and LRP8 in the establishment of retinogeniculate projections. Neural Dev 8:11. [PubMed: 23758727]  [MGI Ref ID J:199170]

Trotter JH; Klein M; Jinwal UK; Abisambra JF; Dickey CA; Tharkur J; Masiulis I; Ding J; Locke KG; Rickman CB; Birch DG; Weeber EJ; Herz J. 2011. ApoER2 Function in the Establishment and Maintenance of Retinal Synaptic Connectivity. J Neurosci 31(40):14413-14423. [PubMed: 21976526]  [MGI Ref ID J:177435]

Uchida T; Baba A; Perez-Martinez FJ; Hibi T; Miyata T; Luque JM; Nakajima K; Hattori M. 2009. Downregulation of functional Reelin receptors in projection neurons implies that primary Reelin action occurs at early/premigratory stages. J Neurosci 29(34):10653-62. [PubMed: 19710317]  [MGI Ref ID J:152314]

Weeber EJ; Beffert U; Jones C; Christian JM; Forster E; Sweatt JD; Herz J. 2002. Reelin and ApoE Receptors Cooperate to Enhance Hippocampal Synaptic Plasticity and Learning. J Biol Chem 277(42):39944-52. [PubMed: 12167620]  [MGI Ref ID J:79593]

Weiss KH; Johanssen C; Tielsch A; Herz J; Deller T; Frotscher M; Forster E. 2003. Malformation of the radial glial scaffold in the dentate gyrus of reeler mice, scrambler mice, and ApoER2/VLDLR-deficient mice. J Comp Neurol 460(1):56-65. [PubMed: 12687696]  [MGI Ref ID J:83151]

Zhang G; Assadi AH; McNeil RS; Beffert U; Wynshaw-Boris A; Herz J; Clark GD; D'Arcangelo G. 2007. The Pafah1b complex interacts with the Reelin receptor VLDLR. PLoS ONE 2(2):e252. [PubMed: 17330141]  [MGI Ref ID J:129338]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use


General Terms and Conditions


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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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