Go to JAX® Mice Query Form

Strain Name:

STOCK Tg(GFAP-TVA)5Hev/J

Stock Number:

003528

Availability:

Repository-Cryopreserved

View Pricing for USA, Canada and Mexico
View Pricing for International

General Terms and Conditions

Genes & Alleles   GFAP;   TVA;   Tg(GFAP-TVA)5Hev;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Wendy Hively,   NIH - NCI
GenerationF15+N1F3N4pN1

Strain Description
This strain expresses the avian cell surface receptor(TVA) for subgroup A avian leukosis viruses. The transgene is under the control of an astrocyte-specific promoter derived from the human glial fibrillary acidic protein (GFAP ) gene. The result is to render glial cells specifically susceptible to infection by viral vectors making possible the glia-specific viral transfer of genes.

Strain Development
The transgenic construct consists of the GFAP promoter, TVA gene (avian cell surface receptor)and a portion of the mouse protamine (Prm1)gene which supplies intronic sequence and a polyadenylation site.

Gene & Allele Details

Allele Symbol Tg(GFAP-TVA)5Hev
Allele Name transgene insertion 5, Harold E Varmus
Common Name(s) Gtv-a;
Mutation Made By Wendy Hively,   NIH - NCI
Strain of Originmixed
Expressed Gene TVA, subgroup A avian leukosis virus receptor, quail
Promoter GFAP, glial fibrillary acidic protein, human
General Note The construct was injected into embryos generated by crossing an FVB/N mouse to a (C57BL/6 x BALB/c)F1 mouse. Glial cells in transgenic mice are specifically susceptible to infection by replication-competent ALV splice acceptor (RCAS) retroviral vectors.
Molecular Note The transgene consists of the astrocyte-specific human glial fibrillary acidic protein (GFAP) promoter, TVA gene (avian cell surface receptor), and a portion of the mouse protamine (Prm1)gene which supplies intronic sequence and a polyadenylation site. The transgene expresses the quail TVA for subgroup A avian leukosis viruses. [MGI Ref ID J:69971]

Genotyping Protocols

Tg(GFAP-TVA)5Hev, Tg(NES-TVA)12Hev

Related Strains

View Strains carrying other alleles of GFAP     (8 strains)

Strains carrying other alleles of TVA
003529   STOCK Tg(NES-TVA)12Hev/J
View Strains carrying other alleles of TVA     (1 strain)

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cancer Research (Gliomas/Glioblastomas, induced)
Developmental Biology Research
Genetics Research (Tissue/Cell Markers: glial cells)
Neurobiology Research

References

Additional References

Price and Supply Information

Strain Name: STOCK Tg(GFAP-TVA)5Hev/J
Stock Number: 003528

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

*NO Shipping Destination selected!

 

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below  

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Go to JAX® Mice Query Form

(2.15)