Strain Name:

STOCK Tg(GFAP-TVA)5Hev/J

Stock Number:

003528

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating InvestigatorDr. Wendy Hively,   NIH - NCI

Description
This strain expresses the avian cell surface receptor(TVA) for subgroup A avian leukosis viruses. The transgene is under the control of an astrocyte-specific promoter derived from the human glial fibrillary acidic protein (GFAP ) gene. The result is to render glial cells specifically susceptible to infection by viral vectors making possible the glia-specific viral transfer of genes.

Development
The transgenic construct consists of the GFAP promoter, TVA gene (avian cell surface receptor)and a portion of the mouse protamine (Prm1)gene which supplies intronic sequence and a polyadenylation site.

Related Strains

View Strains carrying other alleles of GFAP     (20 strains)

View Strains carrying other alleles of TVA     (7 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(GFAP-TVA)5Hev/0

        involves: BALB/c * C57BL/6 * FVB/N
  • nervous system phenotype
  • abnormal glial cell physiology
    • in mice transfected with replication-competent ALV splice acceptor viral vector expressing bFGF glial cells exhibit increased proliferation and migration compared to similarly treated wild-type cells   (MGI Ref ID J:69971)
  • tumorigenesis
  • increased glioma incidence
    • 5 gliomas are discovered in 31 mice transfected with replication-competent ALV splice acceptor viral vector expressing PDGFB   (MGI Ref ID J:125535)
    • increased oligodendroglioma incidence
      • 5 grade II (similar to human oligodendroglioma)   (MGI Ref ID J:125535)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cancer Research
      Gliomas/Glioblastomas, induced
Developmental Biology Research
Genetics Research
      Tissue/Cell Markers
      Tissue/Cell Markers: glial cells
Neurobiology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(GFAP-TVA)5Hev
Allele Name transgene insertion 5, Harold E Varmus
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) Gtv-a;
Mutation Made ByDr. Wendy Hively,   NIH - NCI
Strain of Originmixed
Expressed Gene TVA, subgroup A avian leukosis virus receptor, quail
Promoter GFAP, glial fibrillary acidic protein, human
General Note The construct was injected into embryos generated by crossing an FVB/N mouse to a (C57BL/6 x BALB/c)F1 mouse. Glial cells in transgenic mice are specifically susceptible to infection by replication-competent ALV splice acceptor (RCAS) retroviral vectors.
Molecular Note The transgene consists of the astrocyte-specific human glial fibrillary acidic protein (GFAP) promoter, TVA gene (avian cell surface receptor), and a portion of the mouse protamine (Prm1) gene which supplies intronic sequence and a polyadenylation site. The transgene expresses the quail TVA for subgroup A avian leukosis viruses. [MGI Ref ID J:69971]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(GFAP-TVA)5Hev, Tg(NES-TVA)12Hev, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Tg(GFAP-TVA)5Hev related

Dai C; Celestino JC; Okada Y; Louis DN; Fuller GN; Holland EC. 2001. PDGF autocrine stimulation dedifferentiates cultured astrocytes and induces oligodendrogliomas and oligoastrocytomas from neural progenitors and astrocytes in vivo. Genes Dev 15(15):1913-25. [PubMed: 11485986]  [MGI Ref ID J:77287]

Deshpande A; Bergami M; Ghanem A; Conzelmann KK; Lepier A; Gotz M; Berninger B. 2013. Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult dentate gyrus and olfactory bulb. Proc Natl Acad Sci U S A 110(12):E1152-61. [PubMed: 23487772]  [MGI Ref ID J:194243]

Ding BS; James D; Iyer R; Falciatori I; Hambardzumyan D; Wang S; Butler JM; Rabbany SY; Hormigo A. 2013. Prominin 1/CD133 endothelium sustains growth of proneural glioma. PLoS One 8(4):e62150. [PubMed: 23637986]  [MGI Ref ID J:200555]

Holland EC. 2000. A mouse model for glioma: biology, pathology, and therapeutic opportunities Toxicol Pathol 28(1):171-7. [PubMed: 10669005]  [MGI Ref ID J:60497]

Holland EC; Celestino J; Dai C; Schaefer L; Sawaya RE; Fuller GN. 2000. Combined activation of Ras and Akt in neural progenitors induces glioblastoma formation in mice. Nat Genet 25(1):55-7. [PubMed: 10802656]  [MGI Ref ID J:61890]

Holland EC; Hively WP; DePinho RA; Varmus HE. 1998. A constitutively active epidermal growth factor receptor cooperates with disruption of G1 cell-cycle arrest pathways to induce glioma-like lesions in mice. Genes Dev 12(23):3675-85. [PubMed: 9851974]  [MGI Ref ID J:52161]

Holland EC; Hively WP; Gallo V; Varmus HE. 1998. Modeling mutations in the G1 arrest pathway in human gliomas: overexpression of CDK4 but not loss of INK4a-ARF induces hyperploidy in cultured mouse astrocytes. Genes Dev 12(23):3644-9. [PubMed: 9851971]  [MGI Ref ID J:51558]

Holland EC; Li Y; Celestino J; Dai C; Schaefer L; Sawaya RA; Fuller GN. 2000. Astrocytes give rise to oligodendrogliomas and astrocytomas after gene transfer of polyoma virus middle T antigen in vivo. Am J Pathol 157(3):1031-7. [PubMed: 10980141]  [MGI Ref ID J:64389]

Holland EC; Varmus HE. 1998. Basic fibroblast growth factor induces cell migration and proliferation after glia-specific gene transfer in mice. Proc Natl Acad Sci U S A 95(3):1218-23. [PubMed: 9448312]  [MGI Ref ID J:69971]

Hu X; Pandolfi PP; Li Y; Koutcher JA; Rosenblum M; Holland EC. 2005. mTOR promotes survival and astrocytic characteristics induced by Pten/AKT signaling in glioblastoma. Neoplasia 7(4):356-68. [PubMed: 15967113]  [MGI Ref ID J:100163]

Kermer V; Ritter M; Albuquerque B; Leib C; Stanke M; Zimmermann H. 2010. Knockdown of tissue nonspecific alkaline phosphatase impairs neural stem cell proliferation and differentiation. Neurosci Lett 485(3):208-11. [PubMed: 20849921]  [MGI Ref ID J:167252]

Lassman AB; Dai C; Fuller GN; Vickers AJ; Holland EC. 2004. Overexpression of c-MYC promotes an undifferentiated phenotype in cultured astrocytes and allows elevated Ras and Akt signaling to induce gliomas from GFAP-expressing cells in mice. Neuron Glia Biol 1(2):157-163. [PubMed: 17047730]  [MGI Ref ID J:117003]

Motori E; Puyal J; Toni N; Ghanem A; Angeloni C; Malaguti M; Cantelli-Forti G; Berninger B; Conzelmann KK; Gotz M; Winklhofer KF; Hrelia S; Bergami M. 2013. Inflammation-induced alteration of astrocyte mitochondrial dynamics requires autophagy for mitochondrial network maintenance. Cell Metab 18(6):844-59. [PubMed: 24315370]  [MGI Ref ID J:206138]

Polajeva J; Sjosten AM; Lager N; Kastemar M; Waern I; Alafuzoff I; Smits A; Westermark B; Pejler G; Uhrbom L; Tchougounova E. 2011. Mast cell accumulation in glioblastoma with a potential role for stem cell factor and chemokine CXCL12. PLoS One 6(9):e25222. [PubMed: 21949886]  [MGI Ref ID J:177670]

Tchougounova E; Jiang Y; Brasater D; Lindberg N; Kastemar M; Asplund A; Westermark B; Uhrbom L. 2009. Sox5 can suppress platelet-derived growth factor B-induced glioma development in Ink4a-deficient mice through induction of acute cellular senescence. Oncogene 28(12):1537-48. [PubMed: 19219070]  [MGI Ref ID J:147585]

Tchougounova E; Kastemar M; Brasater D; Holland EC; Westermark B; Uhrbom L. 2007. Loss of Arf causes tumor progression of PDGFB-induced oligodendroglioma. Oncogene 26(43):6289-96. [PubMed: 17438529]  [MGI Ref ID J:125535]

Uhrbom L; Dai C; Celestino JC; Rosenblum MK; Fuller GN; Holland EC. 2002. Ink4a-Arf loss cooperates with KRas activation in astrocytes and neural progenitors to generate glioblastomas of various morphologies depending on activated Akt. Cancer Res 62(19):5551-8. [PubMed: 12359767]  [MGI Ref ID J:79689]

Uhrbom L; Kastemar M; Johansson FK; Westermark B; Holland EC. 2005. Cell type-specific tumor suppression by Ink4a and Arf in Kras-induced mouse gliomagenesis. Cancer Res 65(6):2065-9. [PubMed: 15781613]  [MGI Ref ID J:97154]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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