Description

Strain Information

Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Wendy Hively,   NIH - NCI

Important Note
It is very likely that this strain carries an allele for retinal degeneration.

Description
This strain expresses the avian cell surface receptor(TVA) for subgroup A avian leukosis viruses. The transgene is under the control of a glial progenitor-specific promoter derived from the human nestin (NES) gene. The result is to render glial progenitor cells specifically susceptible to infection by viral vectors making possible the glia-specific viral transfer of genes. Please note: Mice recovered from this cryopreserved colony may have retinal degeneration.

Control Information

	Control
	Noncarrier
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of TVA

003528

STOCK Tg(GFAP-TVA)5Hev/J

View Strains carrying other alleles of TVA     (1 strain)

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cancer Research (Gliomas/Glioblastomas, induced)
Developmental Biology Research
Genetics Research (Tissue/Cell Markers: glial cells)
Neurobiology Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(NES-TVA)12Hev
Allele Name		transgene insertion 12, Harold E Varmus
Allele Type		Transgenic (random, expressed)
Common Name(s)		N-tva; Ntv-1; Ntv-a;
Mutation Made By		Wendy Hively,   NIH - NCI
Strain of Origin		FVB/N
Expressed Gene		TVA, subgroup A avian leukosis virus receptor, quail
Promoter		NES, nestin, human
General Note		Glial progenitor cells in transgenic mice are specifically susceptible to infection by viral vectors.
Molecular Note		The transgene expresses TVA, the receptor for avian retrovirus ALV, subgroup A (ALV-A), from a quail cDNA under control of a modified human nestin promoter that includes the second-intron enhancer - which in adult mice directs expression specifically in early glial progenitor cells - and the herpes simplex virus thymidine kinase (HSV-TK) promoter. Cells presenting this receptor can be infected by replication-competent ALV splice acceptor (RCAS) vectors, permitting cell type specific expression of vector-borne genes. [MGI Ref ID J:52161]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(GFAP-TVA)5Hev, Tg(NES-TVA)12Hev, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Holland EC; Hively WP; Gallo V; Varmus HE. 1998. Modeling mutations in the G1 arrest pathway in human gliomas: overexpression of CDK4 but not loss of INK4a-ARF induces hyperploidy in cultured mouse astrocytes. Genes Dev 12(23):3644-9. [PubMed: 9851971]  [MGI Ref ID J:51558]

Additional References

Tg(NES-TVA)12Hev related

Browd SR; Kenney AM; Gottfried ON; Yoon JW; Walterhouse D; Pedone CA; Fults DW. 2006. N-myc can substitute for insulin-like growth factor signaling in a mouse model of sonic hedgehog-induced medulloblastoma. Cancer Res 66(5):2666-72. [PubMed: 16510586]  [MGI Ref ID J:106701]

Dai C; Celestino JC; Okada Y; Louis DN; Fuller GN; Holland EC. 2001. PDGF autocrine stimulation dedifferentiates cultured astrocytes and induces oligodendrogliomas and oligoastrocytomas from neural progenitors and astrocytes in vivo. Genes Dev 15(15):1913-25. [PubMed: 11485986]  [MGI Ref ID J:77287]

Dunlap SM; Celestino J; Wang H; Jiang R; Holland EC; Fuller GN; Zhang W. 2007. Insulin-like growth factor binding protein 2 promotes glioma development and progression. Proc Natl Acad Sci U S A 104(28):11736-41. [PubMed: 17606927]  [MGI Ref ID J:122969]

Ferletta M; Uhrbom L; Olofsson T; Ponten F; Westermark B. 2007. Sox10 has a broad expression pattern in gliomas and enhances platelet-derived growth factor-B--induced gliomagenesis. Mol Cancer Res 5(9):891-7. [PubMed: 17855658]  [MGI Ref ID J:129378]

Fults D; Pedone C; Dai C; Holland EC. 2002. MYC expression promotes the proliferation of neural progenitor cells in culture and in vivo. Neoplasia 4(1):32-9. [PubMed: 11922389]  [MGI Ref ID J:75757]

Holland EC; Celestino J; Dai C; Schaefer L; Sawaya RE; Fuller GN. 2000. Combined activation of Ras and Akt in neural progenitors induces glioblastoma formation in mice. Nat Genet 25(1):55-7. [PubMed: 10802656]  [MGI Ref ID J:61890]

Holland EC; Hively WP; DePinho RA; Varmus HE. 1998. A constitutively active epidermal growth factor receptor cooperates with disruption of G1 cell-cycle arrest pathways to induce glioma-like lesions in mice. Genes Dev 12(23):3675-85. [PubMed: 9851974]  [MGI Ref ID J:52161]

Holland EC; Varmus HE. 1998. Basic fibroblast growth factor induces cell migration and proliferation after glia-specific gene transfer in mice. Proc Natl Acad Sci U S A 95(3):1218-23. [PubMed: 9448312]  [MGI Ref ID J:69971]

Holmen SL; Williams BO. 2005. Essential role for Ras signaling in glioblastoma maintenance. Cancer Res 65(18):8250-5. [PubMed: 16166301]  [MGI Ref ID J:101615]

Hu X; Pandolfi PP; Li Y; Koutcher JA; Rosenblum M; Holland EC. 2005. mTOR promotes survival and astrocytic characteristics induced by Pten/AKT signaling in glioblastoma. Neoplasia 7(4):356-68. [PubMed: 15967113]  [MGI Ref ID J:100163]

Lassman AB; Dai C; Fuller GN; Vickers AJ; Holland EC. 2004. Overexpression of c-MYC promotes an undifferentiated phenotype in cultured astrocytes and allows elevated Ras and Akt signaling to induce gliomas from GFAP-expressing cells in mice. Neuron Glia Biol 1(2):157-163. [PubMed: 17047730]  [MGI Ref ID J:117003]

Lyustikman Y; Momota H; Pao W; Holland EC. 2008. Constitutive activation of Raf-1 induces glioma formation in mice. Neoplasia 10(5):501-10. [PubMed: 18472967]  [MGI Ref ID J:138670]

McConville P; Hambardzumyan D; Moody JB; Leopold WR; Kreger AR; Woolliscroft MJ; Rehemtulla A; Ross BD; Holland EC. 2007. Magnetic resonance imaging determination of tumor grade and early response to temozolomide in a genetically engineered mouse model of glioma. Clin Cancer Res 13(10):2897-904. [PubMed: 17504989]  [MGI Ref ID J:123859]

Shih AH; Dai C; Hu X; Rosenblum MK; Koutcher JA; Holland EC. 2004. Dose-dependent effects of platelet-derived growth factor-B on glial tumorigenesis. Cancer Res 64(14):4783-9. [PubMed: 15256447]  [MGI Ref ID J:91507]

Shih AH; Holland EC. 2006. Notch signaling enhances nestin expression in gliomas. Neoplasia 8(12):1072-82. [PubMed: 17217625]  [MGI Ref ID J:121511]

Tchougounova E; Kastemar M; Brasater D; Holland EC; Westermark B; Uhrbom L. 2007. Loss of Arf causes tumor progression of PDGFB-induced oligodendroglioma. Oncogene 26(43):6289-96. [PubMed: 17438529]  [MGI Ref ID J:125535]

Uhrbom L; Dai C; Celestino JC; Rosenblum MK; Fuller GN; Holland EC. 2002. Ink4a-Arf loss cooperates with KRas activation in astrocytes and neural progenitors to generate glioblastomas of various morphologies depending on activated Akt. Cancer Res 62(19):5551-8. [PubMed: 12359767]  [MGI Ref ID J:79689]

Uhrbom L; Kastemar M; Johansson FK; Westermark B; Holland EC. 2005. Cell type-specific tumor suppression by Ink4a and Arf in Kras-induced mouse gliomagenesis. Cancer Res 65(6):2065-9. [PubMed: 15781613]  [MGI Ref ID J:97154]

Weiss WA; Israel M; Cobbs C; Holland E; James CD; Louis DN; Marks C; McClatchey AI; Roberts T; Van Dyke T; Wetmore C; Chiu IM; Giovannini M; Guha A; Higgins RJ; Marino S; Radovanovic I; Reilly K; Aldape K. 2002. Neuropathology of genetically engineered mice: consensus report and recommendations from an international forum. Oncogene 21(49):7453-63. [PubMed: 12386807]  [MGI Ref ID J:79667]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Important Note	It is very likely that this strain carries an allele for retinal degeneration.

Control Information

	Control
	Noncarrier
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

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General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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